Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
권호 표지

Immunostimulating Activity by Protoplast Fusants between Ganoderma Iucidum and Lentinus edodes

영지와 표고의 융합체의 면역활성 증강작용

  • Moon, Chul (College of Pharmacy, Seoul National University) ;
  • Hyun, Jin-Won (Department of Pharmacology, College of Medicine, Seoul National University) ;
  • Kim, Ha-Won (Department of Life Science, University of Seoul) ;
  • Shim, Mi-Ja (Department of Life Science, University of Seoul) ;
  • Kim, Byong-Kak (Department of Pharmacology, College of Medicine, Seoul National University)
  • 문철 (서울대학교 약학대학 종합약학연구소) ;
  • 현진원 (서울대학교 의과대학 약리학교실) ;
  • 김하원 (서울시립대학교 생명과학과) ;
  • 심미자 (서울시립대학교 생명과학과) ;
  • 김병각 (서울대학교 약학대학 종합약학연구소)
  • Published : 2000.06.01
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Abstract

On the inter-order protoplast fusants of Lentinus edodes and Ganoderma lucidum was the antitumor activity test performed and the fusant P22 was selected. The hot water extract of the cultured mycelia of P22 were purified by DEAE-cellulose chromatographya and the resulting purified fraction was designated as P22A. It was found to be a proteoglycan whose molecular weight was 47 kDa. When examined for immunopotentiation activity, P22A increased the number of colony forming unit in the bone marrow stem cells to 3-folds. It also potentiated the secretion of nitric oxide in activated macrophages to 2-folds. In humoral immune response, it increased the activities of the alkaline phosphatase in differentiated B cells to 1.6-folds and the number of plaque forming cells to 1.8-folds. In cellular immune response, it restored the depressed response of delayed type hypersensitivity in tumor bearing mice to normal level. These results suggest that P22A have potential to restore the decreased immune activity of the tumor bearing mice to normal level.

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