• 한국식품영양학회지
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• 제24권4호
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• pp.720-724
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• 2011

녹차 농축액을 탄닌 가수분해 효소를 이용하여 가수 분해한 후 기존 녹차 농축액과 비교하여 이화학 및 관능적 검사를 실시하였다. pH, 항산화 능력은 효소분해 전후 유의적인 차이를 나타내지 않았으나, 효소 분해 후 탁도는 유의적으로 더욱 맑아졌다. 녹차 탄닌의 주성분인 카테킨류는 탄닌분해 효소에 의해 분해되어 전체적으로 감소하는 경향을 나타내었다. 갈레이트형 카테킨 성분인 에피갈로카테킨 갈레이트(EGCG)와 에피카테킨 갈레이트(ECG)는 EGC와 EC 그리고 gallic acid로 효소 분해되어 효소처리 녹차 농축액에서는 EGCG와 ECG가 측정되지 않았다. 떫은맛의 관능검사는 효소분해 후 유의적으로 떫은맛의 감소가 발생하였다. 따라서 탄닌분해 효소를 이용하여 녹차 추출물을 분해하면 녹차액은 맑아지지만, 기존 녹차 추출물의 문제점인 떫은맛은 감소되어 다양한 식품에 응용할 수 있는 것으로 나타났다.

• 동아시아식생활학회지
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• 제21권6호
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• pp.882-887
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• 2011

민들레 뿌리를 이용한 기능성 약주 개발의 기초 자료로 활용하고자 민들레 뿌리를 각각 0, 5, 10, 15%로 첨가하여 민들레 약주(기본적특성: pH 4.0~4.2, 산도4.6~4.8%, 알코올14.92~15.78%, 민들레 뿌리 5% 첨가한 약주를 가장 선호함)의 총 플라보노이드와 총 폴리페놀 함량 및 DPPH 라디칼 소거 활성을 이용한 항산화력을 측정하였으며, 활성 산소종 및 유리기에 전자를 공여하는 환원력을 조사하였다. 총 플라보노이드와 총 폴리페놀 함량은 민들레 뿌리 분말 첨가 비율이 증가할수록 높아졌다. 또한, DPPH 라디칼 소거 활성과 환원력에서도 민들레 뿌리 가루의 첨가비율이 높을수록 증가하였다. 이상의 결과로 보아 민들레 뿌리 가루를 첨가한 약주는 높은 생리활성물질과 항산화 활성을 가지고 있어 기능성 약주로서 개발이 가능하리라 판단된다.

• Asian Pacific Journal of Cancer Prevention
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• 제13권11호
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• pp.5843-5848
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• 2012

Aim: Developing antitumor drugs from natural products is receiving increasing interest worldwide due to limitations and side effects of therapy strategies for the second leading cause of disease related mortality, cancer. Methods: The antiproliferative activity of a methanolic extract from the aerial parts of Marrubium persicum extract was assessed with the MCF-7 breast cancer cell line using the MTT test for cell viability and cytotoxicity indices. In addition, antioxidant properties of the extract were evaluated by measuring its ability to scavenge free DPPH radicals. Moreover, the total phenolic and flavonoid content of the extract was determined based on Folin-Ciocalteu and colorimetric aluminum chloride methods. Results: The findings of the study for the antiproliferative activity of the methanolic extract of M. persicum showed that growth of MCF-7 cells was inhibited by the extract in a dose and time dependent manner, where a gradual increase of cytotoxicity effect has been achieved setting out on 200 ${\mu}g/mL$ concentration of the plant extract. The antioxidant assay revealed that the extract was a strong scavenger of DPPH radicals with an $RC_{50}$ value of 52 ${\mu}g/mL$. The total phenolic and flavonoids content of the plant extract was 409.3 mg gallic acid equivalent and 168.9 mg quercetin equivalent per 100g of dry plant material. Conclusion: Overall, M. persicum possesses potential antiproliferative and antioxidant activities on the malignant MCF-7 cell line that could be attributed to the high content of phenolics and flavonoids, and therefore warrants further exploration.

• 농업과학연구
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• 제45권3호
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• pp.499-508
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• 2018

To use mountain ginseng adventitious roots (MGAR), which is superior in functionality compared to ginseng, and to mass produce raw materials as a functional cosmetic material, the antioxidant and cosmeceutical activities of the extracts of MGAR at different ethanol concentrations (0, 20, 40, 60 and 80%) were compared and analyzed. At first, different ethanol concentrations were compared to determine the best solvent for the extraction of phenolic compounds from MGAR. An 80% ethanol extract with 10.07 mg of gallic acid equivalents/g sample and 0.94 mg of quercetin equivalents/g sample was the best solvent in the extraction of phenolic compounds. However, the 60% ethanol extract had the highest antioxidant activity, evident by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, Trolox equivalent antioxidant capacity (35.01 mM Trolox/g sample) and ferric reducing antioxidant power (460.94 mM $FeSO_4/g$ sample). However, the inhibitory activities of the 80% ethanol extract from the MGAR against tyrosinase and elastase were higher than those of the other extracts. The results of this study show that the extract of MGAR can be used as an easily accessible source of natural antioxidant and as a possible cosmetic ingredient in the cosmeceutical industry. However, the components responsible for the antioxidant and cosmeceutical activities of the MGAR extracts are currently unclear. Therefore, it is suggested that further studies should be carried out to isolate and identify the antioxidant and cosmeceutical components in MGAR.

• Journal of Microbiology and Biotechnology
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• 제30권3호
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• pp.359-367
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• 2020

Neurodegenerative disorders in the elderly are characterized by gradual loss of memory and cognitive function. Oxidative stress caused by reactive oxygen species is associated with progressive neuronal cell damage and death in Alzheimer's disease, one of the most common neurodegenerative disorders. An edible brown seaweed, Ecklonia cava, contains a variety of biologically active compounds such as phlorotannins. In this study, we comparatively evaluated the total phenolic content, antioxidant capacity, and neuroprotective effects of the phlorotannin-rich extract from E. cava (PEEC). The total phenolic content of PEEC and dieckol was 810.8 mg gallic acid equivalents (GAE)/g and 996.6 mg GAE/g, respectively. Antioxidant capacity of PEEC was 1,233.8 mg vitamin C equivalents (VCE)/g and 392.1 mg VCE/g determined using ABTS and DPPH assays, respectively, while those of dieckol were 2,238.4 mg VCE/g and 817.7 mg VCE/g. High-performance liquid chromatography results revealed 48.08 ± 0.67 mg dieckol/g of PEEC. PEEC had neuroprotective effects in pheochromocytoma (PC-12) and human neuroblastoma (SH-SY5Y) cells against H₂O₂- and AAPH-induced oxidative damage, partly due to reduced intracellular oxidative stress. PEEC treatment inhibited acetylcholinesterase and butyrylcholinesterase in a dose-dependent manner. Taken together, these findings suggest that PEEC is a good source of antioxidants and neuroprotective materials.

• 한국식품영양과학회지
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• 제39권9호
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• pp.1243-1248
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• 2010

본 연구는 마전자(Strychnos nux-vomica) 추출물의 항산화 활성 및 $\alpha$-glucosidase 저해활성을 측정하였다. 먼저, 마전자를 각각의 용매로 추출하여 페놀 함량을 알아본 결과 100 g당 SNM에서 71.43 mg을 함유하고 있으며, SNE에서는 24.51 mg, SNA에서는 11.17 mg을 함유하고 있었다. DPPH 라디칼 소거능은 SNM, SNE가 1 mg/mL의 농도에서 50% 정도의 활성을 나타내 항산화 효과가 있다는 것을 확인할 수 있었다. 또한 $200\;{\mu}M$ $H_2O_2$에 의한 DNA 손상에 대해 마전자 추출물의 보호 효과를 확인할 수 있었다. SNM의 $\alpha$-glucosidase 저해활성 측정 시 SNM 1 mg/mL의 농도에서 12.8%의 억제 활성을 나타내었고, SNM 처리시간을 증가시킬 경우 그 활성도가 유의적으로 증가하는 것을 보였다. 이상의 결과로 마전자 추출물의 항산화 활성 및 SNM의 $\alpha$-glucosidase 억제 활성을 확인할 수 있었다.

• 한국산림과학회지
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• 제102권4호
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• pp.566-570
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• 2013

정금나무의 기능성 품종을 육성하기 위하여 정금나무 선발 5개체의 열매에 대한 항산화 활성을 측정하였으며, 항산화 성분인 총 페놀 함량을 측정하였다. 총 페놀 함량은 gallic acid를 기준물질로 하여 분석한 결과 15.8~19.9 mg/g 범위로 전체 평균 17.9 mg/g을 나타냈으며, DPPH free radical 소거활성은 400 ppm의 농도에서 69.1~91.4% 범위로 전체평균 84.2%를 나타내었다. 환원력은 0.56~0.96 범위로 전체 평균 0.79를 나타내었다. 상관분석 결과 DPPH free radical 소거활성과 환원력에서 상관계수가 0.969로 매우 높은 정의 상관관계를 나타내었다.

• 원예과학기술지
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• 제33권2호
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• pp.196-201
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• 2015

Lysophosphatidylethanolamine (LPE) affects the quality of flowers, fruits, and other horticultural products. Studies have provided evidence that LPE can accelerate ripening of fruits and prolong shelf-life at the same time. In this study, the influence of LPE on anthocyanin accumulation and phytochemical characteristics of sweet cherry was investigated. LPE ($10mg{\cdot}L^{-1}$) was applied to a commercial sweet cherry c.v. '0900 Ziraat' orchard two and four weeks before harvest for two treatment years (2011 and 2012). Preharvest applications of LPE resulted in significant improvement in both pomological and phytochemical attributes at harvest. LPE treatment led to a 17% increase in fruit weight and a 6% increase in soluble solid content when averaged over two experimental years. Fruit phytochemical content and antioxidant capacity were increased significantly. The average total phenolic content of LPE-treated fruits for the two years was $703{\mu}g$ gallic acid equivalent (GAE)/g fresh weight (g FW) compared to $569{\mu}g$ GAE/g FW in the untreated control. Fruits treated with LPE had a 27% and 16% more anthocyanin than the control fruits in 2011 and 2012. Antioxidant capacity of fruits, as measured by TEAC (Trolox equivalent antioxidant capacity) assay, was 12.5 and $11.4{\mu}mol$ TE/g FW in LPE-treated and untreated control fruits, respectively, when averaged over two experimental years. Our results suggest that preharvest application of LPE may have the potential to increase anthocyanin accumulation, improve fruit quality and enhance phytochemical characteristics of sweet cherries.

• 한국약용작물학회지
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• 제14권2호
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• pp.77-81
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• 2006

As a result of cytotoxic compounds against cancer cell lines from natural sources, senven compounds were isolated from the leaf and twig of Acer okamotoanum Nakai. The compounds (1-7) were identified as ethyl gallate (1), methyl gallate (2), gallic acid (3), trans $resveratrol-3-O-{\beta}-D-glucopyranoside$ (4), acertannin (5), nikoenoside (6), and fraxin (7) by physicochemical and spectroscopic data (including mp, UV, IR, MS, $^1H-NMR,\;^{13}C-NMR$, DEPT, and HMBC) in comparison with those of published papers. All the compounds were tested for their cytotoxic activity against L1210, HL-60, K562, and B16F10 cancer cell lines in vitro by MTT assay method. Compounds 1-3 and 5 showed cytotoxic activity against all tested cell lines with $IC_{50}$ values ranged from 12.5 to $72.2\;{\mu}M$. Of the compounds, methyl gallate (2) exhibited the most potent cytotoxic activity against L1210, HL-60, K562, and B16F10 tumor cells with $IC_{50}$ values of 12.5, 48.3, 22.8, and $22.8\;{\mu}M$, respectively. Other compounds did not show any cytotoxic activity against four cancer cell lines.

• 한국식품영양과학회지
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• 제40권10호
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• pp.1391-1396
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• 2011

어성초(H. cordata Thunb) 건조 분말(0.05 g)을 10 mL의 증류수와 함께 스테인리스 용기에 넣고 밀봉한 후 여러 온도(50, 100, 200, $300^{\circ}C$)에서 10, 30, 60분간 추출하였다. 추출온도와 시간은 어성초 추출물의 중요 성분, 항산화 활성, acetylcholinesterase 저해 활성에 크게 영향을 미쳤다. $300^{\circ}C$에서 30분간 처리한 어성초 추출물에서 가장 높은 페놀 함량($33.34{\pm}0.96\;{\mu}g$ gallic acid equivalents/mL), quercetin 함량($15.75{\pm}0.20\;{\mu}g/mL$), DPPH 라디칼 소거능($44.07{\pm}2.65%$), acetylcholinesterase 저해 활성($36.53{\pm}1.14%$)이 나타났다. 플라보노이드 함량은 $100^{\circ}C$ 10분 추출물에서 $2.55{\mu}g$ quercetin equivalents/mL로 가장 높게 측정되었다. $100^{\circ}C$ 이상의 고온에서 밀폐된 증류수는 높은 압력에서 아임계수 형태로 존재하는데 이러한 아임계수는 고품질의 어성초 추출물을 제조하는데 매우 유용하였다.