• Korean Journal of Plant Resources
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• v.22 no.6
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• pp.528-534
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• 2009

The study of germination and seedling growth characteristics of Neem under different soil and environment conditions was undertaken. The seed germination started 8 days after sowing in commercial bed soil, whereas, delayed germination was observed in sandy-loam (15 days) and sandy (19 days) soil. The highest germination (73.33%) was observed in commercial bed soil in green house, whereas, the lowest germination was observed in sandy soil (16.67%) and sandy-loam soil (8.33%). The seeds in the open field (sandy soil) also showed poor (10%) germination. The mean number of germination seed/day (GD) and seed germination vigor rate (GV) both were highest in the commercial bed soil with 0.733% and 16.67% respectively in the green house, whereas sandy and sandy-loam soil in green house and open field (sandy soil) all showed much lower GD and GV values. The seedling characteristics of nursery revealed that the seedling grown in the growth chamber in commercial bed soil was significantly higher in all the parameters comparing to others grown in green house and open field. The growth was nearly 7 fold in the chamber compared to that of the green house nursery observed in three months old seedlings. Likewise, HPLC analysis revealed that the green house grown seedling contain higher quantity of pigments compare to the chamber grown seedlings. Among the soils used the commercial soil alone or in combination with sandy and sandy-loam soil in the ratio of 2:1:1 respectively with the temperature of $27{\pm}2^{\circ}C$ showed better for Neem nursery preparation.

• Journal of Acupuncture Research
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• v.36 no.2
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• pp.72-79
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• 2019

Background: The aim of this study was to review clinical efficacy of acupuncture treatment of mild cognitive impairment. Methods: Randomized controlled trials that performed acupuncture treatment for mild cognitive impairment were retrieved from 6 online databases (PubMed, Cochrane Library, EMBASE, CNKI, NDSL, OASIS) on September $30^{th}$, 2018. Studies were selected according to inclusion and exclusion criteria, and were reviewed by Risk of Bias assessment. Results: In total, 21 studies were included in this review. All studies were Chinese (19 studies published in Chinese and 2 in English). The sample size, 50 to 100, and the number of treatment times, 20 to 30, were the largest range in all studies. The most treatments performed was 30. The longest treatment period was 56 days, which accounted for 33% of the studies. The most frequently used evaluation index was the Mini Mental State Examination followed by the Montreal Cognitive Assessment, each used 17 times and 15 times, respectively. The most frequently used acupoints were GV20, EX-HN1, GB20, and GV24, which accounted for 47% of total number of acupoints used. In 48% of the studies, needle retention time was 30 minutes. Western medicine treatment was the most common control group. Most studies reported that the intervention group was statistically significantly different to the control group. Conclusion: These results suggest that acupuncture for mild cognitive impairment was effective. However, it is difficult to confirm this conclusion because the quality of most of these studies were of low quality.

• The Journal of Korean Medicine
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• v.44 no.3
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• pp.87-101
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• 2023

Objectives: Many acupuncture points have been suggested for the treatment of Bell's palsy, but information on which acupuncture points are more important in treatment is not provided. This study was conducted to investigate the importance of acupuncture points currently used clinically in Bell's palsy. Methods: By reviewing the full text of 11 books that recorded acupuncture prescriptions from the Qin Dynasty to the Qing Dynasty, the frequency of use of acupuncture points, their meridians, and their location were investigated. Results: The average number of acupuncture points used for local, adjacent and distal points selection was 10.5, 2, 4 respectively. The number of acupuncture points increased from the Qin Dynasty to the Ming Dynasty 《Bojaebang》 and then decreased, but the proportion of local points has been still high. From the Ming Dynasty, ST4, ST6, GV26, and GV24 were mainly have been used as the local points. Except for the GB12, the use of the rest of the acupuncture points gradually decreased in the adjacent points. In the distal acupuncture points, it was summarized to point of lung and large intestine channel until the Ming Dynasty, and LI4 was mainly used in the Qing Dynasty. Yangming meridian has been most often used at the local and distal area except for the adjacent area. Conclusions: In the treatment of facial paralysis, the lower part of the face had the highest proportion among local acupuncture points, and the Yangming had the highest proportion by meridian.

• Journal of Haehwa Medicine
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• v.16 no.1
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• pp.17-29
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• 2007

Objectives : The purpose of this study is to search acupuncture & moxibustion treatment on hemafecia. Methods : We search thirty-seven oriental medical literatures related to hemafecia and arrange the articles according to the etiological cause, pathogenesis, acupuncture & moxibustion treatment. Results : 1. Hemafecia is mainly caused by intrinsic factors such as inadequate diet, habitual drinking, indiscreet sex and others. 2. Hemafecia is mainly due to the intestinal febrile state, but sometimes due to the infirmity of whole body energy including digestive system. 3. Governor Vessel Meridian, Bladder Meridian, Spleen Meridian and Stomach Meridian are frequently used for acupuncture & moxibustion treatment on hemafecia. 4. B57, GV1 are most frequently used for acupuncture & moxibustion treatment on hemafecia. 5. CV12, CV6, S36 are most frequently used for moxibustion treatment on hemafecia. 6. Acupuncture points are mainly distributed throughout the low back, lower abdomen and lower limb.

• Proceedings of the KSR Conference
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• 2005.05a
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• pp.350-355
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• 2005

The running stability and safety of a railway vehicle depends on the design characteristics and the contact condition between wheel and rail. In this paper, numerical simulations using ANSYS and ADAMS were done on the basis of the experimental observations. The results show that 0.6 Hz of the tail car motion is due to the natural mode of car combination of the KTX. The effects of the conicity of wheel and the lateral stiffness of the secondary suspension on the running stability were analyzed numerically using ADAMS/RAIL. The results also show 0.6 Hz as like the experimental observations. And the adoption of the wheel of GV40(${\lambda}=0.025$) brought the sway motion at the tail cars, but XP55(${\lambda}=0.055$) did not when the secondary lateral stiffness of the KTX was greater than 0.3 MN/m.

• Journal of Embryo Transfer
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• v.7 no.1
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• pp.41-47
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• 1992

1. In vitro system, LR and FSR accelerated and facilitated meiotic progression, and LH selectively improved cytoplasmic maturation which is required to promote the formation of a male pronucleus. 2. Caffeine (2mM) in the fetilization medium was required not only for inducing zona penetrating ability of boar also for developing to the male pronucleus of the penetrat- ing spermatozoa in vitro. 3. The germinal vesicle (GV)stage was observed for the first 17.6 hr;germinal vesicle break-down (GVBD)stage between 17.6~26.4 hr ;metaphase I (M-I)from 26.4 - 30. 9hr;anaphase I(A-I)ranged from 30. 9~33.4hr;telophase I(T-I) at 33.4~34.4hr; and metaphase II(M-II) at 34.4-48hr. 4. The addition of 10%(v /v) pig follicular fluid (pFF) to maturation media significantly increased the rate of nuclear maturation of pig oocytes (p<0.01), whereas the rate of nuclear maturation of pig oocytes among three different media did not differ. 5. The presence of a primary culture of POEC promotes in vitro development of early cleavage stage pig embryos.

• Journal of Acupuncture Research
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• v.18 no.6
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• pp.135-140
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• 2001

Objective : To investigate the effects of Acupuncture therapy on the migraineur by way of the change of the Cerebral blood flow. Methods : 6 migraineurs were inserted in acupoints on GV16, G20, S8 during 2 weeks. and, Mean Velocity (MV) and Pulsatility Index (PI) of MCA, PCA, ACA, BA were measured before and after treatments of 2 weeks, respectively. Results : MV of MCA of the migraineurs was decreased very much by 13.3%, and PI was decreased much by 10.4%. However, MV and PI of BA changed little. Conclusion : It is observed that Acupuncture therapy reduces pains of migraineur through decreasing Cerebral blood flow like MCA.

• Journal of Acupuncture Research
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• v.18 no.6
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• pp.225-231
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• 2001

Objective : Modern acupuncture research often utilizes the methodology of animal experiment where animal acupoints(AA) are stimulated and the results observed. There hasn't been any consensus on the AA locations yet, but it is integral for the locations to be defined in detail for any animal experiment. Methods : The review of 24 original articles published in the Journal of Korean Acupuncture and Moxibustion Society for recent one year which utilized animal experiment methodology revealed that AA are located primarily by the method of transposition from human acupoints. Only a few of those AA were defined in detail. ST-36, CV-12, GB-34, BL-23 were among the most frequently used AA. Results & Conculsion : Transpositional Ⅲ acupoint system of mouse is proposed, which includes 3 points of LU meridian, 4 of LI, 10 of ST, 8 of SP, 3 of HT, 4 of SI, 26 of BL, 2 of KI, 4 of PC, 3 of TE, I1 of GB, 6 of LR, 8 of CV and 19 of GV.

• Journal of Animal Science and Technology
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• v.46 no.1
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• pp.69-76
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• 2004

This study was performed to investigate the effect of satllower(Carthamus tinctorius L.) seed yoghurt on osteoporosis. Sham-operated rats(control) were fed basal diet for 6 weeks. Ovariectomized rats were divided into 6 different experimental groups including basal diet group(G I), yoghurt diet group(G II) and 1% to 4% safflower seed yoghurt diet group(GIII to GVI). and fed with the experimental diet for 6 weeks, respectively. The feed intake, feed efficiency ratio and body weight gains of rats fed with the experimental diet for 6 weeks were analyzed, respectively, and the weight. length, strength, mineral content and density in left femur of the animals were determined, respectively. Also, the right femur tissues were observed by Scanning Electron Microscope(SEM). The level of feed intake was not different among all experimental groups. The feed efficiency ratio and body weight gain were high in safflower seed yoghurt groups, and GV and GVI appeared high specially. Density, strength, ash content and minerals content in left femur were similar between control and G I, and increased with increasing the amount of satllower seed in yoghurt. On the other hand, weight and length in left femur showed no difference among all experimental groups. From the results of SEM observation of right femur tissue, GVI showed high healing effects. From the results of this experiment the healing effects of satllower seed yoghurt diet group were higher than those of control and G I. Among the treatments, GVI showed the highest healing effects on the osteoporosis in ovariectomized rats.

• Journal of Embryo Transfer
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• v.17 no.1
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• pp.55-59
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• 2002

The objective of this study is to determine the developmental competence of in vitro matured bovine oocytes after intracytoplasmic sperm injection(ICSI) with frozen-thawed epididymal spermatozoa. The ovaries were obtained from slaughtered Korean native cows. Oocytes matured in vitro for 24 hrs were fertilized by ICSI with frozen-thawed epididymal spermatozoa. After ICSI, a group of oocytes was activated with 7% ethanol fur 5 min, and the other group was not activated. The oocytes were cultured in TCM-199 medium containing hormones and 10% FCS for 24~30 hrs in a incubator with 5% $CO_2$ in air at 38.5$^{\circ}C$. The percentage of oocytes reaching M II after 24 hrs and 30 hrs of incubation were significantly higher(p<0.05) after culture with TCM-199 media(80.0% and 88.3%) than M I(8.3% and 6.7%). The rate of cleavaged embryos to blastocyst obtained by ICSI treated activation oocytes was significantly higher(p<0.05) than that of nonactivation oocytes(22/46, 47.8% vs 10/39, 25.6%). The rates of embryos development to blastocyst obtained by ICSI treated sperm of flesh, epididymal and frozen-thawed epididymal were 24/45(53.3%), 15/40(37.5%), 11/43(25.6%), respectively and these values of fresh sperm injection were higher than frozen-thawed epididymal sperm. We also concluded that embryos can be produced with ICSI of in vitro matured oocytes by ICSI using frozen-thawed epididymal semen.