• 한국철도학회논문집
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• 제7권3호
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• pp.259-263
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• 2004

The running safety of a railway vehicle depends on the design parameters and contact condition between wheel and rail. In this study, the effect of the conicity of wheel tread is analyzed using ADAMS/RAIL software on running situation. Modal analysis shows in 0.6 Hz natural frequency of lateral mode in fully arranged the KTX cars. The excessive vibration of the tail cars occurs in the 17th car as the speed and the stiffness of the secondary suspension increases, and especially for 1/40 conicity of the GV40 wheel. Also, the analysis shows that combination of wheel profile, GV40 for power cars and XP55 for passenger cars can reduce the lateral vibration of the tail cars.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1998년도 Proceedings of UNESCO-internetwork Cooperative Regional Seminar and Workshop on Bioassay Guided Isolation of Bioactive Substances from Natural Products and Microbial Products
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• pp.143-143
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• 1998

Antagonists acting at the glycine site of the NMDA receptor have been gaining safer alternatives for stroke therapy because they have few adverse effect competitive and noncompetitive NMDA antagonists. Therefore, the neuroprotect novel glycine site antagonist KC0244 were evaluated in a rat model of transient comparison with GV150526A in a developmental phase. Middle cerebral artery oc was produced by insertion of a silicone-coated 4-0 nylon monofilament to the o in male Sprague-Dawley rats under isoflurane anesthesia. After 90 or 120 min retracted and the ischemic tissue reperfused. In 90-min MCAO model, GV150526A was administered 30 min before MCAO or immediately after MCAO. In 120-min MC KC0244 or GV150526A (10 mg/kg, i.p.) was administered 1 hr before MCAO or imme MCAO. Infarct volume was measured 24 hr after MCAO using the 2,3,5-triphe chloride staining method. In 90-min MCAO model, treatments with GV1505 significantly reduce infarct volume although they tended to slightly reduce cor approximately 19% compared with the nontreated group. In 120-min MCAO model with GV150526A did not either significantly reduce infarct volume although the reduce total infarct volume by approximately 16% compared with the vehicle-tre However, 1-hr preischemic and immediate treatments with KC0244 reduced total i 39 and 30% (corrected total infarct volume by 44 and 32%), respectively, co vehicle-treated control group. The results suggest that KC0244 can provid against transient focal ischemic damage with greater in vivo potency than GV150

• 대한의생명과학회지
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• 제7권4호
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• pp.173-179
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• 2001

To investigate the ability to decondense sperm head penetrated into cytoplasm of the oocytes and the relationship between this ability and the level of glutatione (GSH) in mouse oocyte at various maturing stages. The fertilizability of oocytes at various stages of maturation the decondensation of sperm nucleus and the formation of male pronucleus, were observed and the levels of GSH were measured in oocyte at same stages. Besides, the relation between fertilizability and level of GSH in oocyte cytoplasm treated with L-buthionine-S, R-sulfoxmine (L-BSO), the inbitor of biosynthesis of GSH, was determined. The decondensation of sperm head was not found in GV stage and L-BSO treated oocytes. In maturing oocytes (GVBD, MI), the decondensation was found, but the formation of male pronucleus was not. The levels of GSH in oocyte cytoplasm were measured; 2.2 pmol per oocyte in the ovulated and the matured in vitro each, 1.0 pmol in GV intact oocyte, 1.3 pmol in GVBD, and 1.5 pmol in MI phase oocyte. In L-BSO treated oocytes the levels of CSH were measured 0.08~o.09 pmol per oocyte, slightly lower than GV stage oocyte. In conclusion, GSH in oocyte is supposed to be synthesized and storaged in cytoplasm during maturation. The failure of decondensation in the cytoplasm of GV stage and L-BSO treated is suggested that GSH is an essential factor in decondensing the sperm head and that the a certain level of GSH, more than in GV oocyte cytoplasm, is required in decondensation.

• Reproductive and Developmental Biology
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• 제28권1호
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• pp.53-57
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• 2004

The study was carried out to investigate the effects of morphology, reproductive cycle, incubation time and activation of oocytes on in vitro maturation of cat oocytes and development of IVM/IVF embryos. The results were summarized as follows: 1. When recovered from ovaries collected at different stages of the reproductive cycle (inactive, follicular and luteal stage), the developmental rates of oocytes to GV and MI stage were 72.5% and 27.5%, 57.5% and 7.5%, 62.5% and 17.5%, respectively. 2. The developmental rates of oocytes with cumulus cells to GV and MI stage in different conditions of incubation (5% $CO_2$ , 95% $O_2$ and 10% $CO_2$, 90% $O_2$) were 70.0% and 27.5%, 52.5% and 20.0%, 55.0% and 12.5%, respectively. 3. The developmental rates to GV and MI oocytes when cultured at different time of incubation (17∼20, 21∼24, 25∼28 and 29∼32 h) were 67.5% and 20.0%, 67.5% and 30.0%, 62.5% and 22.5%, 65.0% and 15.0%, respectively. 4. The fertilization and cleavage rates of freshly collected oocytes with and without cumulus cells were 72.5% and 25.0%, 37.5% and 7.5%, respectively. The rates were greater in oocytes with cumulus cells than those without cumulus cells. 5. The fertilization and cleavage rates of oocytes recovered from ovaries collected at different stages of the reproductive cycle (inactive, follicular and luteal stage) were 75.0% and 25.0%, 40.0% and 7.5%, 50.0% and 15.0%, respectively.

• Journal of Acupuncture Research
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• 제22권6호
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• pp.101-109
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• 2005

Objectives : Pal Hyue Hyul has been mentioned in $Nanky{\hat{o}}ng$ firstly and is a representitive acupoint of 8 parts of biological function of human body. The aim of this study is to finding out the process of formation of Pal Hyue Hyul. Methods : In this study we investigated and summarized all literetures from $Hwangjaenaeky{\hat{o}}ng$ to the today's papers which refer to Pal Hyue Hyul, and then basing on it, suggested our opinion about the correlation. Results & Conclusion : The results and conclusions obtained are as follow: 1. Pal Hyue Hyul is based on the Sahae theory of $Y{\hat{o}}ngchu$ and was formed in 45nan of $Nanky{\hat{o}}ng$. 2. Although Pal Hyue Hyul has been mentioned in $Nanky{\hat{o}}ng$ firstly, but because even $Nanky{\hat{o}}ng$ shows the quickening of Pal Hyue Hyul theory, the birth of Pal Hyue Hyul was necessary from the viewpoint of studing ambience. 3. About the reason for formation to 8 types, we think that all the biological functions of human body were represented as 8. 4. Pal Hyue theory had been suplemented and completed by annotations of successive medical men, for example the arguement about Golhyue, Suhyue. And the reason for the extent from the heat disease of the first phase to the associated disease is that it has been useful in the clinical treatment. 5. Because about whether Suhyue is Ch'imgol or $J{\hat{o}}lgol$, whether $J{\hat{o}}lgol$ is Yangbo(GB38) or $Hy{\hat{o}}njong(GB39)$, whether Golhyue is Teacu'u(GV14) or $Taej{{\hat{o}}}(BL11)$ there in no agreement of opinion among the successive medical men, much more study is necessary.

• 한국발생생물학회지:발생과생식
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• 제11권1호
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• pp.21-29
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• 2007

본 연구진은 선행 연구에서 progesterone receptor membrane component 2 (pgrmc2) 유전자가 미성숙 난자에서 높게 발현하는 것을 발견하였다. 본 연구는 pgrmc2와 pgrmc1 유전자가 쥐의 생식소와 배아의 발달 단계에 따라 어떻게 발현하는지 알아 보기 위해 수행하였다. 이들 유전자는 난소, 정소, 배아, 그리고 난자의 다양한 발달 단계에서 발현하는 것을 알 수 있었다. 쥐 난자 세포막에 프로게스테론 수용체가 존재하는 것은 progesterone-3-O-carboxymethyl oxime-BSA-fluorescein isothiocyanate (P4-BSA-FITC)의 결합을 이용하여 확인하였다. 본 결과는 프로게스테론 수용체의 세포막 구성 요소들이 생쥐의 난자, 배아, 그리고 생식소에서 발현함을 최초로 확인한 연구 결과다. 스테로이드 수용체의 세포막 구성 요소들의 기능, 특히 프로게스테론 수용체의 세포막 구성 요소들의 기능을 알아 보기 위해 추후 연구가 계속되어야 할 것이다.

• 한국자원식물학회지
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• 제25권1호
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• pp.132-141
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• 2012

본 연구는 기능성 작물, 사방용 수종, 토양개량 등 활용성이 뛰어난 갈매보리수나무의 유용 유전자원의 장기보존 및 실생번식에 도움을 주고자 종자의 발아 온도조건 및 전처리에 대한 발아특성을 구명하고자 실시되었다. 갈매보리수나무 종자는 $10{\sim}35^{\circ}C$의 온도조건에 치상한 결과, 15, $20^{\circ}C$에서 발아율이 가장 높았으며, 평균발아일수, 발아속도 및 발아치는 $25^{\circ}C$에서 가장 좋은 결과를 나타내었다. 또한 주요 발아온도를 예측하기 위하여 2차 및 선형 회귀분석모델을 이용하였는데, 발아율을 이용한 2차 회귀분석 모델에서는 기준온도 $0.6^{\circ}C$, 최대온도 $36.4^{\circ}C$, 적정온도 $18.5^{\circ}C$로 나타났으며, 발아가능 온도범위는 $35.8^{\circ}C$이었다. 발아속도를 이용한 선형 회귀분석모델에서는 기준온도 $8.3^{\circ}C$, 최대온도 $35.4^{\circ}C$, 적정온도 $25.3^{\circ}C$로 나타났으며, 발아가능 온도범위는 $27.2^{\circ}C$로 분석모델간 차이를 나타내었다. 갈매보리수나무 종자를 생리적 처리 방법인 예냉, 층적 및 priming을 이용하여 전처리한 후 발아특성을 조사한 결과, 발아율에서는 $CaCl_2$ 300, 400 mM priming 처리구에서 가장 높은 수치를 나타내었다. 평균발아일수는 층적 6, 8주 처리구에서 가장 짧게 나타났으나 대조구 보다 발아율이 낮은 것으로 보아 발아일수의 단축 보다는 발아의 종기 종료로 판단되었다. 발아속도 및 발아치의 경우 예냉 1, 2주 처리구에서 가장 높게 나타났으며 발아율 또한 $CaCl_2$ 300, 400 mM 처리구에 이어 높은 수치를 나타내어 유묘 생산시 양적 질적 측면을 고려했을 때 가장 적정한 조건으로 판단 되었다.

• Journal of Acupuncture Research
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• 제22권1호
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• pp.91-97
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• 2005

2004 년 3월 9일부터 5월 15일까지 경희의료원 침구과 외래를 내원하여 8주간 연구과정을 마친 환자 29명의 환자를 관찰하여 뜸치료 전, 후의 파킨슨병 증상의 호전도를 여러 임상 척도를 이용하여 살펴 본 결과를 다음과 같이 보고하는 바이다. 1. UPDRS 점수는 침치료 전, 4주 후, 8주 후 각각 $35.41{\pm}22.98$, $31,93{\pm}23.44$, $30.75{\pm}24.55$로 나타나 통계적으로 매우 유의한 차이가 있었다 (p<0.01). 2. HY stage의 변화는 4주후(p=0.046)에는 유의한 변화가 있으나, 8주후에는 (0.063) 유의한 변화가 없었다. 3. Schwab과 England에 의한 ADL의 변화는 4 주후, 8주후 모두 유의한 변화가 없었다(p>0.05). 4. FOCQ의 변화는 치료 4주후에는 유의한 차이가 있었으나(p=0.05) 8주 후에는 유의한 차이가 없었다(p=0.13) 5. 뜸치료를 통해 환자에게서 파킨슨병 임상척도의 변화 외에 움직임의 유연성이나, 덜 피로함과 같은 다양한 변화가 나타났다.

• Journal of Acupuncture Research
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• 제22권6호
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• pp.229-239
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• 2005

목적: 알레르기비염은 이환율이 높은 질환이다. 비폐색은 알레르기비염의 주요 증상으로 수면장애, 우울, 주의력 저하, 기억력 감퇴 등을 유발한다. 비염에 대한 침치료는 문헌에 언급되어 있고 임상에서 많이 사용되지만 잘 디자인된 임상연구는 많지 않다. 본 연구는 알레르기비염환자의 비폐색에 대한 침치료의 효과에 대해 알아보고자 하였다. 방법: 본 연구는 단일맹검, 무작위배정, 대조군연구로 치료혈은 영향(Il20), 상성(GV23), 합곡(IL4)으로 하였고, 유효성 평가는 음향비 강통기도검사를 통한 총비강용적, 총비강최소단면적의 변화로 하였다. 결과: 101명의 피험자가 연구를 종료하였으며 연령, 성별, 체중, 신장, 맥박, 호흡수, 지속성 비염의 중증도, 양성반응을 보인 항원의 수에서 대조군과 침치료군에서 차이는 없었다. 침치료군과 대조군 모두에서 치료직후 총비강용적이 유의성(침치료군: p=0.0007, 대조: p=0.0175)있게 증가하였고, 치료15 분후 침치료군에서 대조군에 비하여 증가된 총비강용적이 경계수준의 유의성(p=0.0871)으로 유지되었다. 침치료군과 대조군 모두에서 치료직후 총비강최소단면적이 유의성(침치료군: p<0.0001, 대조군: p=0.0005)있게 증가하였고, 치료15분후 침치료군에서 대조군에 비하여 증가된 총비강최소단면적이 경계수준의 유의성(p=0.0929)으로 유지되었다. 결론: 지속성 알레르기비염의 비폐색에 대한 침치료는 비강용적과 비강단면적을 증가시켜 비폐색을 완화시키는 효과가 있었으나 지속적인 효과에 대하여는 추가적 연구가 필요하다.

• Clinical and Experimental Reproductive Medicine
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• 제31권3호
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• pp.155-168
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• 2004

Objective: Melatonin, which is secreted by pineal gland play an important role in the regulation of ovarian function via seasonal rhythm and sleep in most mammals. It also has a role in the protection of cells by removing toxic oxygen free radicals brought about by metabolism. In the present study, effects of melatonin on the mouse oocyte maturation were examined using two different culture conditions provided with 5% or 21% oxygen concentration. Material and Method: Immature mouse oocytes were obtained from the ovarian follicles of $3{\sim}4$ weeks old ICR strain mice intraperitoneally injected with 5 I.U. PMSG 44 hour before. Under stereomicroscope, morphologically healthy oocytes with distinct germinal vesicle (GV) were liberated from the graafian follicles and collected using mouth-controlled micropipette. They were then cultured for 17 hour at $37^{circ}C$, 5% $CO_2$ and 21% $O_2$ (95% air) or 5% $CO_2$, 5% $O_2$ and 90% $N_2$. New modified Hank's balanced salt solution (New MHBS) was used as a culture medium throughout the experiments. Effects of melatonin were examined at a concentration of $0.0001{\mu}M$, $0.01{\mu}M$ or $1.0{\mu}M$. For the prevention of spontaneous maturation of immature oocytes during culture, dibutyryl cyclic AMP (dbcAMP) and/or hypoxanthine were included in the medium. Results: Under 21% oxygen condition, oocytes cultured in the presence of $0.01{\mu}M$ melatonin showed a significantly higher maturation rates, in terms of germinal vesicle breakdown (95.0% vs 89.0%) and polar body formation (88.1% vs 75.4%), compared to those cultured with $0.0001{\mu}M$ or $1.0{\mu}M$ melatonin. However, no difference was observed in oocytes cultured under 5% oxygen whether they were treated with melatonin or not. In the presence of $0.01{\mu}M$ melatonin, oocytes either cultured under 21% or 5% oxygen exhibited no difference in the polar body formation (85.6% vs 86.7%). However, in the absence of melatonin, oocytes cultured under 21% oxygen exhibited lower polar body formation (74.7%). When oocytes were cultured in the presence of dbcAMP alone or with varying concentrations of melatonin, those treated with both compounds always showed better maturation, i.e., germinal vesicle breakdown and polar body formation, compared to those cultured with dbcAMP alone. At the same concentration of melatonin, however, oocytes exposed to 21% oxygen showed poor maturation than those to 5% oxygen. Similar results were obtained from the experiments using hypoxanthine instead of dbcAMP. Conclusion: Based upon these results, it is suggested that melatonin could enhance the meiotic maturation of mouse oocytes under 21% oxygen concentration, and release oocytes from the meiotic arrest by dbcAMP or hypoxanthine regardless of the concentration of oxygen, probably via the removal of oxygen free radicals.