• Title/Summary/Keyword: GV17

Search Result 84, Processing Time 0.027 seconds

Identification of Protein Candidates in Porcine Oocytes during In Vitro Maturation

  • Lee, Jae-Dal;Cui, Xiang-Shun;Im, Gi-Sun;Seong, Hwan-Hoo;Kim, Nam-Hyung
    • Reproductive and Developmental Biology
    • /
    • v.32 no.2
    • /
    • pp.71-79
    • /
    • 2008
  • Surface-enhanced laser desorption and ionization time-of-flight mass spectrometry (SELDI-TOF MS) is one of the recently developed proteomic technologies which is based on capturing proteins and peptides by chemically modified surfaces and highly sensitive for the analysis of complex biological samples. In the present study, to gain insights into oocyte maturation and early embryo development, SELDI-TOF-MS was used to find the protein candidates that are specifically or prominently expressed in porcine oocytes at the in vitro matured metaphase II (MIIl) and germinal vesicle (GV) stages. By selected CM10 chip, 16 candidates were found to be up-regulated in GV stage oocytes compared with in MII stage oocytes, their molecular weights were 8,180 (2 candidates), 10,226 (5 candidates), 15,767 (5 candidates) and 16,770 (4 candidates) Da respectively. And the expression of 29 candidates were higher in MII than in GV stage oocytes, their molecular weight were 10,832 (3 candidates), 17,743 (8 candidates), 20,122 (3 candidates), 22,131 (3 candidates), 24,857 (7 candidates) and 33,507 (5 candidates) Da, respectively. The expression of selected 13 candidates (0.2 and 1.0 % error tolerances) were analyzed using real time RT-PCR. The proteins that differentially regulated during oocyte in vitro maturation in the pigs may be potential biomarkers of oocyte maturation and quality.

Comparison between C.M.R.T. and acupuncture in the treatment of liver dysfunction (간 기능 이상 치료에 대한 C.M.R.T. 치료 부위(T8 횡돌기)와 경혈과의 비교)

  • Sim Young;Lee Jun-Moo
    • Korean Journal of Acupuncture
    • /
    • v.19 no.2
    • /
    • pp.97-117
    • /
    • 2002
  • Chiropractic is very similar to Oriental Medicine in philosophy on the cause of diseases and in utilization of spinal articulations for diagnosis and treatment. In this paper the spinal area used to treat liver dysfunction in S.O.T. technique, one of chiropractic techniques, was compared to the acupncture points used to cure the same conditions. Because both Oriental medicine and Chiropractic are dealing with autonomic nervous system in regulating abnormal conditions, also the innervation of spinal nerves to those areas was checked. The spinal area that S.O.T. technique utilizes to correct liver dysfunction is transverse processes of T8, which corresponds to B16. Acupncture points from this level down to T12/L1, which are B16, B17, B18, B19, B20, B21, B45, B46, B47, B48, B49, B50, GV6, GV7, GV8 and GV9, all have been applied to control liver function. Apparent discrepency exists in therapeutic areas for liver malfunction between the two natural healing arts. According to the neurology texts, liver is innervated by sympathetic fibers from the 7th-10th thoracic segments and by parasympathetic fibers from vagus nerve. Sympathetic afferent nerves from the liver reach the 7th-12th thoracic spinal cord segments. It can be said all the 7th-12th thoracic spinal cord segments are related to liver function. Therefore the areas used for liver dysfunction in both natural medicine are appropriately selected. However, B16, the Oriental medical equivalent of the main spinal area which is used for lowered liver function in C.M.R.T. Technique, is not utilized as frequent as in Oriental medicine.

  • PDF

Pilot Study of Effect to the Growth after the Administration of Herbal Medicine to the Prepuberty Children (한약 투여가 사춘기 전 소아의 신장성장에 미치는 영향에 대한 예비 연구)

  • Jeong, Min-Jeong;Gok, Su-Yeong;Lee, Seung-Yeon
    • The Journal of Pediatrics of Korean Medicine
    • /
    • v.22 no.3
    • /
    • pp.25-34
    • /
    • 2008
  • Objectives : The aim of this study was to evaluate the effect to the height-growth after the administration of herbal medicine to the prepuberty children Methods : This clinical study has been carried out with 18 children, who visited to the Department of Pediatrics, Dong-eui oriental medical center from January 2005 to August 2008. They had been treated for height-growth with herbal medicine more than 6 months. This study has been carried out by chart-review to see the effectiveness. Height percentile, BMI and GV(Growth Velocity) after medication was compared with the ones before medication. Results : 1. This clinical study has been carried out with 18 children, 12 boys(66.7%) and six girls(23.3%). Their average age was 10.30 years old. 2. Generally total children's average height percentile was increased compared to after medication. Especially eight of them has significantly increasd height percentile(44.4%). 3. Total children's average GV/6months was also increased after medication. Especially ten of them has significantly increasd GV/6months (55.6%). 4. Total children's average BMI also increased (17.01 to 17.85) after medication. Conclusions : Herbal medicine affects to the growth of pre-puberty children.

  • PDF

Study on the Factors Influencing Fertilization and Developmental Rate of in vitro Cultured Cat Oocytes Recovered from Ovaries Collected at Different Stages of the Reproductive Cycle (번식주기의 단계별로 회수한 고양이 난자의 체외발생에 미치는 요인에 관한 연구)

  • Quen, J. H.;M. H. Lee;S. K. Kim
    • Reproductive and Developmental Biology
    • /
    • v.28 no.1
    • /
    • pp.53-57
    • /
    • 2004
  • The study was carried out to investigate the effects of morphology, reproductive cycle, incubation time and activation of oocytes on in vitro maturation of cat oocytes and development of IVM/IVF embryos. The results were summarized as follows: 1. When recovered from ovaries collected at different stages of the reproductive cycle (inactive, follicular and luteal stage), the developmental rates of oocytes to GV and MI stage were 72.5% and 27.5%, 57.5% and 7.5%, 62.5% and 17.5%, respectively. 2. The developmental rates of oocytes with cumulus cells to GV and MI stage in different conditions of incubation (5% $CO_2$ , 95% $O_2$ and 10% $CO_2$, 90% $O_2$) were 70.0% and 27.5%, 52.5% and 20.0%, 55.0% and 12.5%, respectively. 3. The developmental rates to GV and MI oocytes when cultured at different time of incubation (17∼20, 21∼24, 25∼28 and 29∼32 h) were 67.5% and 20.0%, 67.5% and 30.0%, 62.5% and 22.5%, 65.0% and 15.0%, respectively. 4. The fertilization and cleavage rates of freshly collected oocytes with and without cumulus cells were 72.5% and 25.0%, 37.5% and 7.5%, respectively. The rates were greater in oocytes with cumulus cells than those without cumulus cells. 5. The fertilization and cleavage rates of oocytes recovered from ovaries collected at different stages of the reproductive cycle (inactive, follicular and luteal stage) were 75.0% and 25.0%, 40.0% and 7.5%, 50.0% and 15.0%, respectively.

The Research about Checking Method of Governor Vessel's Acupoints at Lumbar Regions : (compared with X-ray measuring) (요부(腰部) 독맥경(督脈經)의 혈위(穴位) 결정방법(決定方法)에 관한 연구(硏究)(X-선상(線像)을 중심(中心)으로))

  • Choe, Ick-Seon
    • Journal of Acupuncture Research
    • /
    • v.17 no.4
    • /
    • pp.1-4
    • /
    • 2000
  • For checking the exact acupuncture points of Governor Vessel(GV) at lumbar regions, five kinds of method were used to 100 patients who have treated in outpatient in our hospital. They were attached distinguishable stainless steel by the methods. And those were compared with X-ray measuring. As a results, the best method to check Yoyang-gwan(GV3, Ya~oyanggua~n) was the way of using both iliac crests at lateral lying position.

  • PDF

A Case Report of a Tremor Patient with Stenosis of the Left Carotid Artery (머리 떨림 환자에 대한 한방 치험 1례)

  • Park, Seo-hee
    • The Journal of Internal Korean Medicine
    • /
    • v.38 no.6
    • /
    • pp.1096-1100
    • /
    • 2017
  • The carotid artery is one of the main vessels supplying blood to the brain. Carotid artery stenosis is mostly caused by atherosclerosis, a disease where cholesterol is deposited in the arterial blood vessels. Tremor refers to rhythmic shaking of a body part. Tremor is a symptom of many diseases, including Parkinson's disease, essential tremor, orthostatic tremor, cerebellar disease, peripheral neuropathy, and alcohol withdrawal. Tremors may be classified as postural, rest, and action tremors. Tremor of a patient with stenosis of the left carotid artery decreased with acupuncture treatment. The acupoints were GB20, TE17, GV8 and GV11. This case shows that the acupuncture treatment is effective against tremor.

The Research Trends of Pharmacopuncture Therapy on Cervical Pain Caused by Traffic Accidents

  • Kim, Dong-young;Hong, Seung-hyo;Han, Soo-yeon;Kim, Won-young;Oh, Seo-hye;Lee, Hyung-woo;Woo, Hyun-su
    • Journal of Pharmacopuncture
    • /
    • v.23 no.4
    • /
    • pp.201-211
    • /
    • 2020
  • Objectives: The purpose of this study is to comprehensively review Korean domestic studies and investigate the research trends of pharmacopuncture therapy on cervical pain caused by traffic accidents. Methods: Domestic studies between February 1999 and May 2020 from four Korean databases were searched with combinations of keywords 'cervical pain', 'traffic accident', 'whiplash injury', and 'pharmacopuncture'. Results: 17 studies were selected for review, including 7 randomized controlled trials, 5 retrospective observational studies, 3 case reports, and 2 non-randomized controlled trials. Each study was reviewed by published year, study type, types of pharmacopuncture solutions, selected acupuncture points, dosage of pharmacopuncture solutions, frequency of treatment, concurrent treatments, outcome measurements, and the effectiveness of pharmacopuncture therapy. The results are as follows: (1) It showed that the total number of published studies had increased slightly in the last 10 years compared to the previous decade. (2) The pharmacopuncture solutions used in the studies were in the following order: Bee-Venom (蜂毒), Jungsongouhyul (中性瘀血), Hwangryunhaedoktang (黃連解毒湯) and Soyeom (消炎). Frequently used acupuncture points were GB20, GV21, A-shi point, GV16, EX-B2, and SI15 in order. (3) The most commonly used total injection dosage was 1.0 cc at a time, and the frequency of treatment was twice a week. (4) Concurrent treatments such as acupuncture, herbal medication, physical therapy and Chuna manual therapy were performed in all 17 studies. (5) Pharmacopuncture therapy showed positive effects on cervical pain caused by traffic accidents in all 17 studies reviewed. Conclusion: Pharmacopuncture therapy was effective in cervical pain caused by traffic accidents in all 17 studies selected. Further studies will be needed using more larger scales and more objective data to confirm the effectiveness of pharmacopuncture therapy and to generalize its application.

A Review of Clinical Studies for Treatment of Multiple System Atrophy Using the CNKI Database (다계통 위축증의 치료에 대한 임상연구 동향 - CNKI검색을 중심으로)

  • Kim, Min-ji;Won, Seo-young;Kim, Hae-yoong;Ryu, Ju-young;Jung, Eun-sun;Yoo, Ho-ryong;Seol, In-chan;Kim, Yoon-sik
    • The Journal of Internal Korean Medicine
    • /
    • v.41 no.4
    • /
    • pp.612-623
    • /
    • 2020
  • Objectives: The aim of this research was to investigate and review the trends in recent clinical studies of multiple system atrophy (MSA) in China. Methods: We searched for clinical studies discussing Oriental medicine-based treatments for MSA in the China National Knowledge Infrastructure (CNKI) database. The search focused on the authors; publication year; type of study; method and duration of treatment; evaluation criteria; and results of the selected articles. Results: Among the articles retrieved from the database, eight case series, two randomized controlled trials (RCTs), and one non-randomized controlled trial (nRCT) were analyzed. Eight articles used acupuncture; ten used herbal medicine. The most frequently used acupoints were GV20, GV24, GB20, PC6, Hyeopcheok Acupoint, CV4, EX-HN3, and GV17. Jihwangeumja was the most commonly used herbal prescription. All 13 studies confirmed the efficacy of Oriental medicine treatments. Conclusions: More varied and scientifically designed clinical studies are required to develop treatments for MSA. The results of this study may provide guidance for further studies of MSA.

The Effects of Nicotine on the Mouse Oocyte Maturation In vitro (생쥐 난자의 체외 성숙에 미치는 Nicotine의 영향)

  • Sung, Ki-Cheong;Bae, In-Ha
    • Clinical and Experimental Reproductive Medicine
    • /
    • v.28 no.1
    • /
    • pp.1-12
    • /
    • 2001
  • Objective: The present study was done to clarify the effects of nicotine and nicotine tartrate on the mouse oocyte maturation in vitro. Methods: GV (germinal vesicle) oocytes were isolated from Graafian follicle of ovaries with sharp needles under a stereomicroscope from female mouse of ICR strain (4 weeks old). Collected oocytes were cultured for 17 hours at $37^{\circ}C$, 5% $CO_2$ in air and 100% humidified condition in incubator. New MHBS was the basic medium used in which nicotine, nicotine tartrate, and mecamylamine (antagonist of nicotinic acetylcholine receptor) were added depending on the experimental group. GV oocytes were cultured in one of these media. Results: Nicotine ($300{\mu}M{\sim}5mM$) had no effects on GVBD (germinal vesicle breakdown) compared to the control, but increasing concentration of nicotine led to an decrease in the first polar body formation. However, nicotine ($10{\sim}500{\mu}M$) induced GVBD in a dose-dependent manner of GV oocytes in a medium containing dbcAMP. Nicotine tartrate ($50{\mu}M{\sim}5mM$) had no effects on GVBD compared to the control but, increasing concentration of nicotine tartrate led to an decrease in the first polar body formation. Mecamylamine $10{\mu}M$ added to the medium containing nicotine ($300{\mu}M{\sim}5mM$) showed higher percentage of the first polar body formation compared to the nicotine ($300{\mu}M{\sim}5mM$) treatment group. Mecamylamine $10{\mu}M$ added to the medium containing nicotine tartrate ($50{\mu}M{\sim}5mM$) showed higher percentage of the first polar body formation compared to the nicotine tartrate ($50{\mu}M{\sim}5mM$) treatment group. Conclusion: The present study suggest that nicotine and nicotine tartrate have the harmful effects on the meiotic maturation of the mouse oocytes in vitro. However, mecamylamine block harmful effects of nicotine and nictine tartrate.

  • PDF

A Philological Study on the Pathology, Acupuncture and Moxibustion of Alopecia (탈발(脫髮)의 병인병기(病因病機) 및 침구치료(鍼灸治療)에 관한 문헌고찰)

  • Lee, Sang Hyun;Cho, Myoung Rae
    • Journal of Acupuncture Research
    • /
    • v.32 no.2
    • /
    • pp.35-57
    • /
    • 2015
  • Objectives : The purpose of this study is to analyze literature on oriental medicine to investigate the pathology, acupuncture, and moxibustion therapy applicable to alopecia Methods : We searched for the causes of alopecia in the bibliographic data from 41 sources of literature and the acupunctural treatments for alopecia in the bibliographic data from 49 sources of Chinese and Korean literature and from the journals of the acupunctural treatments for alopecia which have been published since 2000. Results : 1. The main causes of alopecia are blood deficiency, blood heat, static blood, depletion of kidney's water, wind excess, seven emotional damage, dietary damage, etc. 2. The acupuncture points frequently used are $GV_{20}$, $GB_{20}$, $ST_{36}$, $SP_6$, $SP_{10}$, $BL_{17}$, $BL_{18}$, $BL_{23}$, $GV_{23}$, $GV_{14}$, $CV_6$, $ST_8$, $KI_3$, $LR_3$, $HT_7$, $LI_{11}$, EX-$HN_1$, and ouch point. 3. New acupuncture therapies, including herbal acupuncture therapy, ear acupuncture therapy, dermal needle therapy, needle-embedding therapy and scalp acupuncture therapy are frequently used to treat alopecia. Conclusions : The acupuncture points frequently used for treating alopecia have characteristics such as tonifying blood, tonifying the liver and kidney, extinguishing wind, activating blood, clearing heat and draining dampness, so they are highly associated with the causes and the mechanisms of alopecia.