• 한국양식학회:학술대회논문집
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• 한국양식학회 2003년도 추계학술발표대회 논문요약집
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• pp.25-25
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• 2003

어류의 번식활동은 뇌-뇌하수체-생식소로 이어지는 내분비기관이 주로 담당하고 있으며, 그 중에서 뇌하수체에서 만들어지는 생식선자극호르몬 (GTH)은 생식소 발달에 매우 중요한 역할을 한다. 한편, GTH는 생식소에서 생성되는 스테로이드호르몬에 의해 유전자의 발현이 조절된다. 본 연구에서는 산천어 (Oncorhynchus masou)의 GTH 유전자발현에 미치는 estradiol-17$\beta$ (E2)의 영향을 생체내에서 먼저 조사하고, 유전자발현조절의 메카니즘을 구명하기 위하여 에스트로젠 수용체 (ER)의 cDNA를 cloning하였다. 또한 내분비교란물질로 알려져 있으며 에스트로젠 수용체와의 유사결합작용이 알려진 노닐페놀 (NP)의 영향도 아울러 조사하였다. 미성숙 산천어를 E2 및 NP (각각 5 mg/kg 및 10 mg/kg 체중)로 처리하면 72 시간째에 뇌하수체내의 $\beta$-actin mRNA에 비해 GTH (FSH$\beta$, LH$\beta$) mRNA가 상대적으로 증가하는 경향을 나타내었다. 뇌하수체에서 분리한 ER의 기본적인 구조는 DNA결합도메인 (Zf-C4), 호르몬결합도메인 (hormone_rec), 기타 전사활성화도메인으로 구성되어 있었으며 (그림 1), 기존에 보고된 에스트로젠 수용체중에서 무지개송어 및 대서양연어의 ER과 각각 98%, 96%의 높은 상동성이 관찰되었다. 향후, 준비된 ER을 재료로 하여 GTH 유전자발현의 호르몬의존성을 조사할 예정이다.

• 한국가축번식학회지
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• 제19권4호
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• pp.251-258
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• 1996

These studies were carried out to investigate the effect of gonadotropins (GTH), fetal calf serum (FCS), porcine follicular fluid (pFF) and FCS and pFF fractions obtained by the gel filtration on in vitro maturation of porcine follicular fluid. When the oocytes were cultured in TCM-199, the maturation rate was higher in pFF than in FCS in both with or without GTH and in pFF the maturation rate was higher in with GTH than in without GTH. In case of without GTH, pFF increased maturation rates in TCM-199, but not in Whitten's medium (WM). When the oocytes were cultured in WM supplemented with FCS fractions, the maturation rate(51.6%) of oocytes was significantly (P<0.05) higher in fraction B (about 30∼70 kDa) than in control, FCS and other fractions. When oocytes were cultured in WM supplemented with pFF fractions, fractions B (about 30∼70 kDa) and D (about 1∼10 kDa) were significantly (P<0.05) higher than in control, pFF and other fractions. In conclusiion, the addition of gonadotropins into the maturation media was effective for oocyte maturation. The addition of pFF was more effective than addition of FCS for maturation of porcine oocytes in vitro. And fraction B from FCS and fractions B and D from pFF was effective for oocyte maturation.

• 한국발생생물학회지:발생과생식
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• 제26권1호
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• pp.13-21
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• 2022

Neurokinin B (NKB) is a neuropeptide involved in the regulation of reproductive endocrine system of vertebrate animals, including fish. However, the pathway of NKB action in fish has not been clearly elucidated. In order to clarify the effect of NKB and NKF (neurokinin F) on gonadotropic hormone (GTH) gene expression in the pituitary, we studied the changes of LHβ and FSHβ gene expressions by using two different pituitary culture methods (whole pituitary culture or dispersed pituitary cell culture). Pituitaries were removed from mature female and male Nile tilapia. Changes of LHβ and FSHβ gene expressions were measured and compared after the treatment with NKB or NKF peptides at concentrations 0 to 1,000 nM. Expression of GTH genes in the whole pituitary cultures treated with NKB or NKF peptides did not show significant difference except in female at one concentration when treated with NKF. On the contrary, there were significant changes of GTH gene expressions in the dispersed pituitary cell cultures when treated with NKB and NKF peptides. These results suggest that dispersed pituitary cell culture is more relevant than whole pituitary culture in studying the function of pituitary, and that NKB and NKF could act directly on the pituitary to regulate the expression of GTH genes.

• 한국양식학회:학술대회논문집
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• 한국양식학회 2006년도 수산관련학회 공동학술대회 발표요지집
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• pp.273-274
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• 2006

• Communications for Statistical Applications and Methods
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• 제28권5호
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• pp.477-491
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• 2021

The Markov modulated Brownian motion is a substantial generalization of the classical Brownian Motion. On the other hand, the Markovian arrival process (MAP) is a point process whose family is dense for any stochastic point process and is used to approximate complex stochastic counting processes. In this paper, we consider a superposition of the Markov modulated Brownian motion (MMBM) and the Markovian arrival process of jumps which are distributed as the bilateral ph-type distribution, the class of which is also dense in the space of distribution functions defined on the whole real line. In the model, we assume that the inter-arrival times of the MAP depend on the underlying Markov process of the MMBM. One of the subjects of this paper is introducing how to obtain the first passage probabilities of the superposed process using a stochastic doubling algorithm designed for getting the minimal solution of a nonsymmetric algebraic Riccatti equation. The other is to provide eigenvalue and eigenvector results on the superposed process to make it possible to apply the GTH-like algorithm, which improves the accuracy of the doubling algorithm.

• 한국발생생물학회지:발생과생식
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• 제12권3호
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• pp.261-266
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• 2008

연어과 어류인 열목어 재조합 생식선자극호르몬(r-mtFSH 또는 r-mtLH) 투여에 따른 암컷 뱀장어(Anguilla japonica)의 성성숙 유도 효과를 조사하였다. 양식산 암컷 뱀장어를 해수에 적응시킨 후 매주 복강에 재조합 호르몬을 농도별(0.1, 1, 10 ${\mu}g/m{\ell}$/fish)로 10주 반복 주사하였다. 매주 증체중을 측정하였고, 생식소중량지수 [GSI;(생식소중량/체중량$\times$100]와 혈중 성호르몬 농도 변화를 조사하였다. 그 결과, 모든 실험군에서 GSI는 서서히 감소하는 경향을 나타내었다. 또한, 대조구에서 혈중 testosterone (T)과 estradiol-17$\beta$(E2)는 유의적인 증가를 보이지 않았지만, 재조합 호르몬을 투여한 실험군에서 투여2주와 4주 후에 T와 E2 농도가 증가하였다. 또한, mt-rFSH(1, 10 ${\mu}g/m{\ell}$/fish) 또는 mt-rLH(0.1, 1, 10 ${\mu}g/m{\ell}$/fish)을 투여한 실험군에서는 난경이 대조구에 비해 유의하게 증가하였다. 이러한 결과는 열목어 재조합 생식선자극호르몬이 암컷 뱀장어의 초기 난성숙 발달을 유도함을 시사한다.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 1997년도 수산관련공동학술대회발표요지집
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• pp.189-190
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• 1997

• Archives of Pharmacal Research
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• 제20권1호
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• pp.68-71
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• 1997

Penicillin sensor was prepared by immobilizing penicillinase (Pcase) on $H^{+}$-selective carboxylated poly (vinyl chloride) (PVC-COOH) membrane or cellulose filter membrane. The immobilization techniques are as follows. Pcase was immobilized with GTH on $H^{+}$-selective PVC-COOH membrane or some amount of BSA was dropped on that membrane. Another method to make immobilization is to mix type I Pcase with GTH and drop on a cellulose filter membrane. According to immobilization techniques, there were some differences in response properties of enzyme electrodes, however, all electrodes responded to Pcase-resistant penicillin derivatives. Pcase immobilized on cellulose filter membrane with $H^{+}$-selective PVC membrane eletrode was more stable and more sensitive to penicillinase-resistant penicillin derivatives than any other immobilization techniques.

• 한국수산과학회지
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• 제50권6호
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• pp.770-776
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• 2017

This study investigated the effects of recombinant eel gonadotropin hormone (rJeGTH) produced in silkworms, with and without a carboxyl-terminal peptide from equine chorionic gonadotropin (eCG), on the induction of sexual maturation in female eels Anguilla japonica. Experiments were conducted both in vivo and in vitro. In in vitro trials, germinal vesicle breakdown (GVBD) induction did not significantly differ between rJeFSH and $rJeFSH{\cdot}eCG$ treatments and the control group. However, previous studies did find that rJeLH and $rJeLH{\cdot}eCG$ treatments induced GVBD in female eels. Our in vitro exploration of $estradiol-17{\beta}$ ($E_2$) levels in immature ovarian tissues revealed significantly higher $E_2$ levels in the group treated with $rJeFSH{\cdot}eCG$ $1{\mu}g/mL$ than in the control group. In contrast, the in vivo experiments showed no effect of recombinant hormones on the sexual maturation of feminized eels. Previous studies and our own in vitro results have clearly shown that rJeGTH and $rJeGTH{\cdot}eCG$ have a positive effect on the sexual maturation of feminized eels. To develop the activity of rJeGTH in vivo, further studies should confirm circulation time and activity of these hormones in eels' bloodstream, modify the structure of the recombinant gene, and implement additional glycosylation.

• 한국가축번식학회지
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• 제19권1호
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• pp.15-34
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• 1995

1970년말부터 뇌하수체 성선자극호르몬(gonadotropic hormone ; GTH)의 유전자 구조(FSH$\beta$, LH$\beta$ 및 공동의 $\alpha$쇄)가 다양한 종에서 밝혀지기 시작하였으나 이러한 유전자의 조직/세포 특이적 분비양식과 세포외 신호에 의한 조절양식은 정확히 밝혀져 있지 않다. 그러나 최근 들어 형질전환 맞추스 제작기법에 의해 $\alpha$쇄 유전자 상류에 세포특이적 발현을 조절하는 특이부위가 존재함이 보고됨을 시작, FSH$\beta$ 및 LH$\beta$쇄 유전자발현을 조절하는 특이부위 또한 가까운 시기내 발견되리라 기대된다. 한편, 성선자극 호르몬 방출호르몬(GnRH), 스테로이드 호르몬 및 여러 결합단백질과 같은 세포의 신호는 각기 다른 신호전달체계를 통하여 GTH유전자 발현을 일으킨다. 또한 뇌하수체에서도 그 존재가 확인된 전사인자들 (cFos, cJun)과 미지의 인자들은 상호간에 다양한 이량체를 형성하여 유전자 발현을 조절하는 각 특이부위에 결합함으로써 전사단계에서의 다양한 제어가 존재함이 밝혀지고 있으며 이러한 유전자상의 특이발현영역과 세포의 신호별 전사인자에 관한 연구는 번식에 있어 중요한 성선자극호르몬에 관한 분자수준의 조절기전을 밝혀내리라 기대되어진다.