Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Induction of Sexual Maturation in Female Eels Anguilla japonica by Recombinant Eel Gonadotropin Produced in Silkworm

누에에서 생산된 뱀장어 재조합 생식소자극호르몬이 암컷 뱀장어(Anguilla japonica)의 성성숙 유도에 미치는 영향

  • Kim, Dae-Geun (Aquaculture Research Institute, National Institute of Fisheries Science) ;
  • Kim, Hyo-Won (Aquaculture Research Institute, National Institute of Fisheries Science) ;
  • Kim, Jung-Hyun (Aquaculture Research Institute, National Institute of Fisheries Science) ;
  • Lee, Bae-Ik (Aquaculture Research Institute, National Institute of Fisheries Science) ;
  • Hong, Sun-Mee (Department of Research and Development, Institute of Gyeongbuk Marine Bioindustry) ;
  • Min, Kwan-Sik (Animal Biotechnology, Graduate School of Future Convergence Technology, Institute of Genetic Engineering, Hankyong National University) ;
  • Myung, Jeong-In (Aquaculture Research Institute, National Institute of Fisheries Science) ;
  • Kim, Dae-Jung (Aquaculture Research Institute, National Institute of Fisheries Science)
  • 김대근 (국립수산과학원 전략양식부 양식관리과) ;
  • 김효원 (국립수산과학원 전략양식부 양식관리과) ;
  • 김정현 (국립수산과학원 전략양식부 양식관리과) ;
  • 이배익 (국립수산과학원 전략양식부 양식관리과) ;
  • 홍선미 (경북해양바이오산업연구원) ;
  • 민관식 (한경대학교 생물정보통신대학원) ;
  • 명정인 (국립수산과학원 전략양식부 양식관리과) ;
  • 김대중 (국립수산과학원 전략양식부 양식관리과)
  • Received : 2017.11.16
  • Accepted : 2017.12.11
  • Published : 2017.12.31
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Abstract

This study investigated the effects of recombinant eel gonadotropin hormone (rJeGTH) produced in silkworms, with and without a carboxyl-terminal peptide from equine chorionic gonadotropin (eCG), on the induction of sexual maturation in female eels Anguilla japonica. Experiments were conducted both in vivo and in vitro. In in vitro trials, germinal vesicle breakdown (GVBD) induction did not significantly differ between rJeFSH and $rJeFSH{\cdot}eCG$ treatments and the control group. However, previous studies did find that rJeLH and $rJeLH{\cdot}eCG$ treatments induced GVBD in female eels. Our in vitro exploration of $estradiol-17{\beta}$ ($E_2$) levels in immature ovarian tissues revealed significantly higher $E_2$ levels in the group treated with $rJeFSH{\cdot}eCG$ $1{\mu}g/mL$ than in the control group. In contrast, the in vivo experiments showed no effect of recombinant hormones on the sexual maturation of feminized eels. Previous studies and our own in vitro results have clearly shown that rJeGTH and $rJeGTH{\cdot}eCG$ have a positive effect on the sexual maturation of feminized eels. To develop the activity of rJeGTH in vivo, further studies should confirm circulation time and activity of these hormones in eels' bloodstream, modify the structure of the recombinant gene, and implement additional glycosylation.

Keywords

References

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