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Effect of Cyclohexane and Xylene Mixture Treatment on the Liver Damage in Rats

  • Shin, Joong-Kyu
    • Biomedical Science Letters
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    • v.9 no.2
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    • pp.93-98
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    • 2003
  • To investigate the cyclohexane and xylene mixture treatment on the liver damage, the rats were treated by the mixture of cyclohexane and xylene (CH+X) and then, liver damage was demonstrated by liver function findings based on liver weight/body weight, serum level of alanine aminotransferase (ALT), xanthine oxidase (XO) and then compared with cyclohexane treated group (CH group) and xylene-treated group (X). The CH+X group showed merely severer liver damge than CH or X group. On the other hand, CH+X group showed lower activity of hepatic cytochrome P-450 dependent aniline hydroxylase (CYPdAH) than CH or X group, but no statical differences were demonstrated among three experimental groups. Especially the hepatic GSH content was merely declined than CH or X group and the activity of hepatic GST was higher in CH+X group than CH or X group. In conclusion, cyclohexane and xylene mixture treated animals showed merely severer liver damage than cyclohexane or xylene treated group and such a fact may be caused by inhibition of cyclohexane or xylene metabolism and oxygen free radical.

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THE EFFECTS OF ND:YAG LASER AND IRRIGANTS ON CANAL SEALING ABILITY (근관치료시 Nd:YAG Laser 사용과 세척액에 따른 치근단 폐쇄효과의 비교)

  • Kim, Jin-Woon;Lee, Hee-Ju;Hur, Bock
    • Restorative Dentistry and Endodontics
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    • v.26 no.4
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    • pp.307-315
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    • 2001
  • The application of Nd:YAG laser and irrigants to the root surface can change its surface configurations. The purpose of this study was to investigate the effects of Nd:YAG laser and irrigants on the apical seal of obturated canals. In this study, 66 single rooted teeth were randomly assigned to 4 group of 14 teeth each. 8 teeth were served us positive and negative controls. The teeth were divided into 6 groups as follows. Group A: Nd:YAG laser, 5% NaOCl + Rc-prep Group B: Nd:YAG laser, Saline Group C: 5% NaOCl + Rc-prep Group D: Saline Group E: Positive control Group F: Negative control 66 teeth were instrumented using Maillefer ProFile$^{\circledR}$ (Orifice Shapers, .04 taper, .06 taper Dentsply, Switzerland). Two of each group were selected at random, and the canal wall surfaces were examined under a SEM. 12 teeth of each group were obturated using by lateral condensation technique. Specimens were immersed in india ink for 7days, decalcified by 10% nitric acid, dehydrated by 75. 80. 85, 90, 95 and 100% alcohol in order cleared by methyl salicylate and then measured of dye penetration with stereomicroscope($\times$15 magnification) and Image Pro plus. The data were analyzed statistically by one-way ANOVA test and Duncan's Multiple Range test. The results were as follows : 1. The mean leakage was 0.128$\pm$0.376 for group A, 0.237$\pm$0.325 for group B, 0.397$\pm$0.468 for group C, 0.586$\pm$0.402 for group D, and there were statistically significant differences between group A and group D, group B and group D. (p<0.05). 2. Group A had better sealing ability than Group C, but there was statistically no significant differences. (p>0.05). 3. Group B had better sealing ability than Group D and there was statistically significant difference. (p<0.05). 4 Group A had better sealing ability than Group B, but there was statistically no significant difference. (p>0.05). 5. Group C had better sealing ability than Group D, but there was statistically no significant difference. (p>0.05). 6. As a result of observation under SEM, Smear layers were removed in Group A, B. but Smear layers were partially removed and smear plugs were remained in Group C, Smear layers were not removed in Group D. To be specially, Melting of smear layer were showed in Group C. 7. These results suggests that the laser has a potential in reducing the apical microleakage of obturated canals.

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THE STUDY OF THE DENTAL PLAQUE FORMATION ON DIFFERENT IMPLANT SURFACES (임플란트 표면에 따른 인공치면세균막 형성에 관한 연구)

  • Kim, Jin-Woo;Han, Se-Jin;Kim, Kyung-Wook
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.34 no.3
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    • pp.325-340
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    • 2008
  • The purpose of this study was to evaluate the artificial dental plaque by Streptococcus mutans on 4 different implant surfaces. In this study, the specimens were divided into 4 groups according to implant surface treatment. Uncoated implant group(n=5) which has an uncoated, smooth surfaced implant(Osstem, Korea), SLA implant group(n=5) which has an sandblasted large grit and acid-etched surface implant(Bicon, USA). Oxidized implant group(n=5) which has an oxidized surfaced implant (Osstem, Korea), and RBM implant group(n=5) which has resorbable blasting media(RBM) surfaced implant(Osstem, Korea). Acquired pellicle by human saliva and dental plaque by Streptococcus mutans were made on each implant surface. To analyze the plaque condition on implants surfaces, cell count and optical density were taken as a microbiologic method, and SEM(Scanning Electronic Microscope) findings was also taken for evaluation of surface condition. The following results were obtained. 1. Cell counting results of artificial dental plaque were Uncoated group($658.0{\pm}102.0$), RBM group($878.0{\pm}170.0$), SLA group ($946.0{\pm}42.0$), Oxidized group($992.0{\pm}40.0$), and there was difference between Oxidized group and Uncoated implant group(p<0.05). In case of modified cell counting results by v/w% were RBM group($197.8{\pm}45.2$), Oxidized group($207.04{\pm}8.34$), Uncoated group($261.6{\pm}40.6$), SLA group($315.4{\pm}14.0$), and there was difference between RBM group and SLA group(p<0.05). 2. Optical density results of artificial dental plaque after ultrasonic treatment was that there was difference among groups, and optical density of RBM group was higher than that of Uncoated group(p<0.05). In case of modified optical density results by v/w%, there was difference among groups, and the modified optical density of Uncoated group and SLA group was higher than those of Oxidized group and RBM group(P>0.05). 3. SEM findings of artificial dental plaque on the surfaces of implant as follows; there were artificial dental plaque on the surfaces of all test implants. Streptococcus mutans and by-product were observed at 10,000 times magnified condition on all test implants. Adhesion area of artificial dental plaque was about 1/2 of total surface after 24 hours incubate at $37^{\circ}C$. These results showed that there were differences among implant surfaces on the growth of Streptococcus mutans, and bacteria and by-product were covered about 1/2 area of total implant surfaces at 24 hours incubate at $37^{\circ}C$.

A Study on Value Orientation of Health of Rural Health Center Milieu (농촌지역 보건소 환경에 있어서의 건강가치관에 관한 일 조사연구)

  • 김순자
    • Journal of Korean Academy of Nursing
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    • v.5 no.1
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    • pp.17-30
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    • 1975
  • The concept and definition of nursing and her role have been changing in accordance with the socio-cultural factors of the initial society. At present, nursing is conceptualized as a health care profession assisting man to restore, maintain and promote health by providing knowledge, wilt strength and resources through various processes of interaction. Man′s behavior, of individual and group activities for health inclusive, is driven by the initial man′s value orientation. The purpose of this study is to investigate the value orientation regarding health of rural health center milieu in order to give data for; 1. the planning for the delivery of community nursing service, 2. the health education plan at all level of nursing care activities, and 3. the planning of curriculum for nursing education. A hundred opinion leaders among the labor population residing in rural areas (P-group), hundred and six professional nurses at rural health centers (N-group) were indirectly interviewed through questionaries. And ninety five of N-group were interviewed likewise of their perceptions of P-group (NP-group) from July 15, to October 15, 1974. The result is as follows: 1. Maintenance of health is revealed to be the most valued component for man′s happiness in all the three groups. (P-group: 7.30 S. D.=1.31), (N-group :7.84 S. D. =49), and (NP-group : 5.93 S. D. =2.28) 2. The average value score of the maintenance of health revealed significant difference by P〈.001 level between each of the three groups. (Between N-group and P-group : T= -4.07 P and NP ; T=-6.93, N and NP: T=-9.35) 3. Basic health maintenance activities necessary for maintenance and promotion of personal health were moderately valued by all the three groups, P-group ; 3.74 (S. D. =.43) , N-group: 3.52 (S. D. =.34), NP-group: 3.07 (S. D. :.55) Among the 8 categories of basic health maintenance activities, "food intake" was highly valued by P-group (mean value score; 4.00 S. D=.51) , "exercise and rest" and "personal cleanliness" was highly valued by both P-group (4.02, 4.08) and N-group (4.08, 4.22). 4. The mean value score of basic health maintenance activities revealed significant difference by P〈.001 level between each of the three groups (between P-group and N-group: T=-4, 07, N-and NP: T=-6.93, P and NP T=-9.35) 5. Among the 30 questionaries, dynamic activities for health maintenance were more valued in comparison to passive activities in an tile three groups. 6. In N-group, correlation between the value of basic health maintenance activities and the personal health status personal revealed moderate significance. Correlation between the value of basic health maintenance activities and the age revealed low significance. 7. In group, correlation between the value of basic health maintenance activities and; perception of personal health status revealed non significance, between the age and sex revealed low sign affiance, and between the educational status revealed moderate significance. Recommendations are as follows ; 1. The efforts towards alteration of value orientation concerning health in general in community nursing practice de focussed be that of family Planning. 2. In order to prepare professional nurses competent in understanding individual and group, social science and behavioral science be strength ended in planning nursing curriculum. 3. Milieu of nursing experience during nursing education be Planned to begin at simple nursing problem and move towards complex, f. e. home care towards health crisis situation in order to achieve dynamic role mastery.

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The perception and wearing attitude toward school uniform by group according to clothing attitude - Focusing on high school students -

  • Kim, Ju Ae
    • The Research Journal of the Costume Culture
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    • v.22 no.6
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    • pp.899-910
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    • 2014
  • The purpose of this study was to analyze high school students' school uniform wearing attitude by group according to clothing attitude targeting Gyeongnam area. This study aims to provide preliminary data in the field of school uniform and marketing that clothing propensity by groups is considered. This study conducted a survey targeting 762 high school students in Gyeongnam. For statistical analysis, SPSS for Window 14.0 was used for frequency analysis, factor analysis, reliability analysis, multiple sponse analysis, cluster analysis, ANOVA analysis and Duncan's ex-post analysis method. As a result of cluster analysis on the clothing attitude, students were divided into 4 segmentation of fashion seeking group, fashion indifference group, conformity group and modesty group. As a result of verification on the difference in perception toward wearing school uniform by groups which were classified according to the propensity of clothing attitude, activity, stability, and practicality were all varied according to the propensity of clothing attitude. 4 groups were significant differences in the degree of consent to wearing school uniform, price of school uniforms, tendency to prefer famous brand when purchasing school uniform, experience of transforming school uniform, opinion about school uniform modification and reason for school uniform modification. While low graders were many in 'modesty group', upper graders were many in 'fashion seeking group', which means that more segmentalized satisfaction of clothing by group may be raised if such a fact is considered when planning clothing for high school students segmentalized by age.

The Effect of Stress During Pregnancy on the Skin Barrier of Mice Four Weeks After Birth (임신 중 만성스트레스가 생후 4주 유서(幼鼠)의 피부장벽에 미치는 영향)

  • Cha, Jae-Hoon;Kim, Yoon-Bum
    • The Journal of Korean Medicine
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    • v.30 no.1
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    • pp.83-94
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    • 2009
  • Background and Objectives: The skin barrier protects skin against a harmful environment. Its function includes an antimicrobial barrier as well as a physical barrier. Stress is harmful to the skin barrier and there are many studies on this, but there are few about the effect of stress during pregnancy on the skin barrier of offspring. The aim of this study was to investigate the effect of stress during pregnancy on the skin barrier of offspring by examination with the naked eye, cortisol, weight, TEWL and histologic tests. Materials and Methods: Male hairless mice ten weeks old were coupled with females for three days. After twelve days the females were divided into two groups. We stressed one group and not the other group. The offspring from the non-stressed (N group) and stressed (S group) grew up until four weeks. The S group was exposed to chronic mild stress using the chronic stress model protocol modified method of Wilner and Towell for a week. We made examinations with the naked eye, assessment of cortisol, weight, TEWL, and histologic test to contrast the S group with the N group. Statistical analysis was performed by using Mann-Whitney and Friedman test. Statistical significance was achieved if the probability was less than 5% (p<0.05). Results: 1. By making an examination with the naked eye, S group showed papules, creases and dryness on their skin, but N showed no change compared with normal skin. 2. There was no statistically significant difference between S group and N group in cortisol (p>0.05). 3. There was no statistically significant difference between S group and N group in weight. N group had less weight than S group (p<0.05). 4. There was no statistically significant difference between S group and N group in TEWL (p>0.05). 5. There was no statistically significant difference between S group and N group in TEWL recovery (p>0.05). 6. In TEWL and TEWL recovery, there was statistically significant difference by time between S group and N group (p<0.005). Histologically, S group showed a thicker epidermis and epidermal crest than N group. Conclusions: We found that stress during pregnancy has negative effects on the skin barrier of offspring. Though there were no statistically significant differences between S group and N group in TEWL recovery, we found evidence that the recovery of skin barrier function is interrupted by stress during pregnancy by examination with both the naked eye and histology.

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Comparative Enzymatic Hydrolysis of Bacillus amyloliquefaciens DS11 Phytase and Aspergillus ficuum Phytase in the Cannulated Pigs (Cannula를 장착한 돼지에서 Bacillus amyloliquefaciens DS11 Phytase와 Aspergillus ficuum Phytase의 역가 비교)

  • 장범수;박승춘;윤효인
    • Journal of Veterinary Clinics
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    • v.18 no.1
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    • pp.18-21
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    • 2001
  • In this study, we assessed the efficacy of a novel B. amyloliquefacience DS11 phytase (DS11 phytase) and that of a commercial Aspergillus ficcum phytase (AF phytase) through their bioavailabilities of phytin-posphorus and -calcium in the diet using cannulated pigs. For the purpose of evaluating the efficacy of the phytases in pigs, we determined phosphorous concentrations from serum and feces, in addition to ingesta obtained from the cannula at the terminal ileum. As results, phosphorus concentration was lower in feces from DS11 group and BASF group by 17% and 10%, and higher in serum from the respective groups by 34% and 20%, as compared to the control group. Both phytases are evaluated to enhance phosphorus availability to the great extent. Calcium concentration of feces were lower in DS11 group and BASF group by 31% and 10%, than that in the control. Calcium concentration of serum was higher in DS11 phytase group by 4% but lower in AF phyase group by 3%, then that in the control group.

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Analysis of the Effect of Training Program for Parent-Adolescent Communication Applied to Home Economics Classes, and the Possibility to Implement the Program (가정과 수업에의 적용 가능성 탐색을 위한 부모자녀간 의사소통훈련 프로그램의 효과분석)

  • 박인순
    • Journal of the Korean Home Economics Association
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    • v.36 no.4
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    • pp.109-120
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    • 1998
  • The Purpose of this research was to introduce communication training program for positive parent-adolescent relation into home economics education curriculum. The research applied the program named "Making the desirable world 2" which was revised by K.E.T.I(Korea Effectiveness Training Institute). This program was given two hours a week during 8 weeks. The subject of this study was 24 students, divided into two groups, the experimental and the control group. This research supposed that parent-adolescent communication and self-esteem in the experimental group would significantly increase by group training program. The results of this research are as follows. First, parent-adolescent communication in the experimental group was significantly increased by the group training program. Second, self-esteem in the experimental group was significantly increased by the group training program. For home economics education as a practical subject matter, promoting qualitative maturity of students' family life, this study aimed both at enhancing communication for positive relation of parent-adolescent by group training and implementing home economics education curriculum practically.

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Effect of Sheep and Chicken Antibodies to Rat Adipocytes Plasma Membranes on Rat Carcass Fat

  • Wang, Jundong;Zhang, Jianfeng;Li, Junping;Hao, Junhu;Wang, Shaolin;Zhang, Jianhai
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.8
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    • pp.1177-1182
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    • 2004
  • Polyclonal anti-sera were collected from sheep and chicken immunized with adipocytes plasma membranes. Thirty two male wistar rats, weighing 185-215 grams, were divided randomly into 4 groups (trial 1: control group and treat group, trial 2: control group and treat group), with 8 rats in each group. The experiment lasted for 7 weeks. Trial one: The control group received four consecutive daily intraperitoneal injections of 1ml of sheep normal sera. The same 4 day daily dose of group sheep anti-rats sera adipocyte plasma membrane anti-sera was administered to the treat group. The results showed that the treatment for treat group increased body weight by 6.35% (p<0.05) and food intake by 6.85%, and improved food conversion efficiency (Food intake/gain) by 45.00% (p<0.05). Periernal, epididymal and omental adipose deposit weights were decreased by 23.92% (p<0.05), 34.45% (p<0.05) and 0.98% respectively, while total fat content decreased by 20.92%. Trial two: The control group received four consecutive daily intraperitoneal injections of 1 ml of chicken normal sera, the results of injections of chicken anti-rats sera adipocyte plasma membrane antis-era administered to the treat group indicated that chicken anti-rats adipocyte plasma membranes immunization had an disadvantageous effect on the growth of the wistar rats by the end of 7th wk, compared with the control group. The immunized group decreased in total weight by 40 gram (p<0.05) an averagely and in food intake noticeably (p<0.01). The deposition of fat and the rates of TG and FFA in serum had no statistical significance.

Evaluation of Obesity from BMI and Waist Circumference, and Its Relation with Cardiovascular Risk Factors (건강검진 수진자들의 비만유형과 관상동맥질환 위험인자와의 관련성)

  • Cho, Ju-Yeon;Park, Jae-Yong;Han, Chang-Hyun
    • Korean Journal of Health Education and Promotion
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    • v.25 no.2
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    • pp.47-59
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    • 2008
  • Objectives: The objective of this study was held for prevention of coronary artery disease and improvement of health of local community residents by classifying the obesity types of their waist circumference and BMI, and appraising the coronary artery disease risk factors(CRF). Methods: We analyzed the data on the 1,914 adult cases (1,156 male and 758 female) during Nov, 2006 to Mar. 2007 on a general hospital in Daegu city, Korea. The obesity types in this research were classified into normal group, seeming obesity group, abdominal obesity group and obesity group. Also, CRF was classified by normal, mild, moderate, and severe, and each class was given the index from 0 to 3. The coronary artery disease risk factors index(CRFI) was defined as the sum of index, and we defined that if the sum is higher, higher chance of coronary artery disease risk exist. Results: According to the research, by the age group, normal group has higher percentage in age 30 to 40, and obesity group and abdominal obesity group has higher percentage in age 50 to 60. CRFI is increased by order of normal group, seeming obesity group, abdominal obesity group, and obesity group on both male and female groups, and male shows higher index than female on all four groups. CRFI will be influenced by order of obesity group, seeming obesity group, and abdominal obesity group on male, and obesity group, abdominal obesity group, and seeming obesity group on female according to the result of multiple regression between obesity type and CRFI. Conclusion: According to the result from our research, we have to have keen attention to not only seeming obesity group and obesity group, but also abdominal obesity group which has normal waist circumference influence to CRFI. So, those kinds of indexes have to be controled by controling their weight. Also, we believe that health behaviour can improve and CRF can be prevented by the early health care and early health education to those with no abnormal indication on clinical indicator but with abnormal BMI and waist circumference.