Comparative Enzymatic Hydrolysis of Bacillus amyloliquefaciens DS11 Phytase and Aspergillus ficuum Phytase in the Cannulated Pigs

Cannula를 장착한 돼지에서 Bacillus amyloliquefaciens DS11 Phytase와 Aspergillus ficuum Phytase의 역가 비교

  • 장범수 (충남대학교 수의과대학 약리독성학실험실) ;
  • 박승춘 (경북대학교 수의과대학 약리독성학실험실) ;
  • 윤효인 (충남대학교 수의과대학 약리독성학실험실)
  • Published : 2001.03.01

Abstract

In this study, we assessed the efficacy of a novel B. amyloliquefacience DS11 phytase (DS11 phytase) and that of a commercial Aspergillus ficcum phytase (AF phytase) through their bioavailabilities of phytin-posphorus and -calcium in the diet using cannulated pigs. For the purpose of evaluating the efficacy of the phytases in pigs, we determined phosphorous concentrations from serum and feces, in addition to ingesta obtained from the cannula at the terminal ileum. As results, phosphorus concentration was lower in feces from DS11 group and BASF group by 17% and 10%, and higher in serum from the respective groups by 34% and 20%, as compared to the control group. Both phytases are evaluated to enhance phosphorus availability to the great extent. Calcium concentration of feces were lower in DS11 group and BASF group by 31% and 10%, than that in the control. Calcium concentration of serum was higher in DS11 phytase group by 4% but lower in AF phyase group by 3%, then that in the control group.

Keywords

References

  1. Asian-Australasian J Anim Sci v.10 Apparent digestibility of amino acids, energy and proximate nutrients in grain sources and tapioca for young pigs Cho SB;Kim JH;Yang JS;Moon HK;Chae BJ;Han IK
  2. Biotechnology in Feed Industry(1st ed) Pig Production: managing maximum feed efficiency with minimum pollution Cole DJA
  3. J Anim Sci v.71 Efficacy of phytase in improving the bioavailibility of phosphorous in soybean meal and corn-soybean meal diets for pigs Cromwel GL;Stahly TS;Coffey RD;Monegue HJ;Randolph JH
  4. J Anim Sci v.71 Efficacy of phytase in improving the bioavailability of phosphorous in soybean meal and corn-soybean meal diets for pigs Cromwell GL;Stahly TS;Coffey RD;Monegue HJ;Randolph JH
  5. J Bio Chem v.68 The colorimetric determination of phosphorus Fiske CH;Subbarow YP
  6. Inositol Phosphate(2th ed) phytate Irving GCJ
  7. Brit J Nutr v.70 Phosphorus studies in pigs. 3. Effect of phytase supplementation on the digestibility and availability of phosphorus in soya-bean meal for grower pigs Ketaren PP;Batterham ES;Dettmann EB;Farrell DJ
  8. FEMS Microbiol Lett v.162 Cloning of the thermostable phytase gene (phy) from Bacillus sp. DS11 and its overexpression in Escherichia Kim YO;Lee JK;Kim HK;Yu JH;OhTK
  9. J Anim Sci v.71 Supplementing corn-soybean meal diets with microbial phytase linearly improves phytate phosphorus utilization by weanling pigs Lei XG;Ku PK;Miller ER;Yokoyama MT
  10. J Anim Sci v.72 Apparent digestibility and retention of nutrients bound to phytate complexes as influentced by microbial phytase and feeding regimen in pig Morz Z;Jongbloed AW;Kemme PA
  11. J Agri Sci Finl v.62 Microbial phytase supplementation for improving availability of plant phosphorus in the dite of the growing pigs Nasi M
  12. Biotechnology in Feed Industry(1st ed) Pig and poultry: waste management and pollution control Pettigrew JE
  13. Advances in food Research v.28 Phytates in legumens and cereals Reddy NR;Sathe SK;Salunkhe DK
  14. J Anim Sci v.71 Addition of microbial phytase to diets of young pigs Young LG;Leunissen M;Atkinson JL
  15. 서울대학교 대학원 박사학위논문 Phytase가 단의가축의 성장능력 및 인의 이용성에 미치는 영향 권 관
  16. 한국축산학회지 v.40 미생물 phytase의 첨가 육성돈과 비육돈의 성장에 미치는 효과 권관;권찬호;정흥우;유문일;손광수;현영;박승춘
  17. 한국수의공중보건학회지 v.23 Bacillus amyloliquefaciens DS11 phytase의 욱계 위장관에서 인농도에 미치는 효과 장범수;윤효인;박승춘;김민규;최양웅;오태광