• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.212.2-212.2
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• 2003

Primary cultured rat hepatocytes injured by carbon tetrachloride as a model to screen for hepatoprotective effect. Four flavonoid compounds showed anti-hepatotoxic effect by decrease GPT. LDH activity and MDA level. Also screen for hepatoprotective, anti-oxidative and anti-apoptosis effects of baicalin and baicalein on chang cell treated with t-BHP. Mesured radical detoxifying enzyme, GST and antioxidant enzyme SOD, Catalase activity, GSH level and Cellular glutathion peroxidase activity. (omitted)

• 약학회지
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• 제44권3호
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• pp.283-292
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• 2000

Angelicae gigantis Radix aqua-acupuncture solution (AGRAS) and Angelicae gigantis Radix water-extracted solution (AGRWS) were prepared and tested for their organ toxicities and chemopreventive potentials. The organ-toxicity of AGRAS to male ICR mice was studied by the measurements of glutamic oxaloacetic transaminase (GOT), glutamic pyruvate transaminase (GPT), lactate dehydrogenase (LDH) and alkaline phosphatase (ALP-s) activities after injection of AGRAS for 7 days. The activities of GOT GPT and LDH were decreased, but the activity of ALP-s was not changed with AGRAS. When AGRAS was administered once daily for 10 days before the tumor implantation, AGRAS exerted antitumor activity by inhibiting the growth of Ehrich ascites tumor cells (EATC) in viva. The inductions of quinone reductase (QR), glutathione (GSH) and glutathione S-transferase (GST) and inhibition of polyamine metabolism were tested for the chemopreventive potentials of AGRAS and AGRWS. AGRAS was potent inducer of QR activity in murine hepatoma Hepalclc7 cells. In cultured rat Ac2F cells, AGRAS was also significantly induced QR activity GSH levels were increased about 1.3 fold with AGRAS. In addition the activity of GST was increased about 2.5 fold with AGRAS at the concentration of $0.1{\;}{\times}{\;}$. The effects of AGRAS and AGRWS were tested on the growth of Acanthamoeba castellanii. Proliferation of Acanthamoeba castellanii in a broth medium was inhibited by AGRAS and AGRWS at the concentration of $1{\;}{\times}{\;}and{\;}5{\;}{\times}{\;}$, respectively: These results suggest that AGRAS has chemopreventive potential by inducing QR activity increasing GSH and GST levels and inhibition of polyamine metabolism.

• 한국응용과학기술학회지
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• 제1권1호
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• pp.23-31
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• 1984

In order to investigate the acute toxicity of autoxidized methyl linoleate(AOML) on the activity of serum enzymes in the mouse, we administered once 0.45ml of AOML to ICR strain mouse by using stomach tube. The following results were obtained: The total lactic dehydrogenase(LDH) activities in the serum of AOML group were generally increased than those of normal group. According to electrophoresis, the activities of LDH, were increased while those of LDH, were decreased. The activities of glutamic oxaloacetic transaminase(GOT), glutamic-pyruvic transaminase(GPT) and ${\alpha}-amylase$ in the serum of AOML group were increased more than those of normal group. The activities of alkaline phosphatase in the serum of AOML group were increased but those of isozyme were not confirmed in the normal and AOML group. In the serum protein of AOML group, albumin was increased, on the other hand ${\gamma}-globulin$ was decreased. At the peripheral blood slide smear, lymphocytes were significantly decreased but neutrophils were increased and the morphological change of erythrocytes was observed. From these results we conclude that the AOML fed to mouse influences on the activity of various serum enzymes and blood cells in the mouse.

• Toxicological Research
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• 제9권2호
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• pp.253-262
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• 1993

The effects of dichloromethane (DCM) on carbon tetrachloride (CT) toxicity were examined in adult male rats. A concomitant treatment of rats with DCM (0.3, 0.6, 1.2 g/kg, po) significantly potentiated the hepatotoxicity of CT (1.0 g/kg, po) as determined by increase in serum GPT (glutamic pyruvic transaminase), GOT (glutamic oxaloacetic transaminase), and SDH (sorbitol dehydrogenase) activity 24 hr following the treatments. Serum LDH (lactate dehydrogenase) activity was increased by either DCM or CT treatment.

• 생약학회지
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• 제14권1호
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• pp.17-23
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• 1983

To experimentally research out the effect of 'Hagocho-San' used for liver disease on the clinical treatment of oriental medicine, I induced liver damage rats by thioacetamide and dosed the extracts orally and measured the activity rate of GOT, GPT, ALP and LDH. The following results were obtained: 1) GOT activity was more decreased in the experimental group than in the control group. Extracts from mixed decoction was more efficacious than those mixed form of the extracts after individual decoction. Extracts from decoction showed continuous significance after 48 hours on, but mixed form of extracts after individual decoction was significant only after 48 hours and 96 hours. 2) GPT activity was more decreased in the experimental group than in the control group. Extracts from mixed decoction was effective after 48 hours on but mixed form of extracts after individual decoction was significant after 48 hours and 72 hours. 3) ALP activity was more decreased in the experimental group than in the control group. Extracts from mixed decoction was more efficacious than the mixed form of extracts after individual decoction and significance was verified after 72 hours on. 4) The significance of LDH activity in the extracts from mixed decoction was revealed after 72 hours and 96 hours but mixed form of extracts after individual decoction showed significance 72 hours later. 5) From the above results, it was showed that 'Hagocho-San' extract was effective on liver disease and extracts from mixed decoction was more effective than mixed form of extracts after individual decoction.

• Archives of Pharmacal Research
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• 제13권4호
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• pp.298-305
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• 1990

Based on equal number of cells, supplementation of 10$^{-6}$ M cadimium highly simulated the intracellular amylase. GCT, LDH as well as the glucose and erea content of E carotovora var, carotovaro cells. This was coupled with initiation of highly active GOT, CPK as well as accumulation of cholesterol in the cells. Lanthanum was less active and unable to initiate COT or CPK. Nickel was almost without effect though reduced LDH activity without initiating either enzyme or cholesterol production. Similar stiulations and/or initiations were observed, though to variable extents, when the same concentration of the three elements were supplied to E. carotovora var, citullis or E. toxica. The highest yield of amylase, GPT, GGT or glucose was obtained when E. carotovora var. carotovora was supplemented with Cd + Ni. The highest urea level was recorded in Erwinia carotovara var, cirullis, amended with Cd + La.

• 동국한의학연구소논문집
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• 제5권
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• pp.53-77
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• 1996

The purpose of this study is to observe the effect of Sayeuksan on serum reaction in $CCl^4$ treated rats. In this study the experimental rats divided four group (Control group, $CCl^4$ group, sayeuksan group, and shiho, kamcho group) Under the same condition control group were administered water, sample group were administered Sayeuksan for 8days. and then, both $CCl_4$ group and sample group were injeccted to abdomen with $CCl_4$ for 1days. The change of GOT GPT ${\gamma}$-GTP ALP LDH activity in blood serum. The obtained results are summarised as follows : 1. In the change of GOT GPT contents, as compared with control group sample group was significantly decreased. 2. In the change of ${\gamma}$-GTP contents as compared with control group, sample group was significantly decreased. 3. In the change of ALP contents, as compared with control group, sample group was significantly decreased. 4. In the change of LDH contents, as compared with contol group, sample group was significantly decreased.

• 한국식품영양과학회지
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• 제15권3호
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• pp.235-242
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• 1986

chloroform으로 인한 간(肝) 및 위장조직(胃臟組織)에 미치는 영향을 알기 위해 rat에 농도별로 Chloroform을 투여(投與)하고, 또 이들에 화분립(花粉粒)을 함께 투여(投與)한 결과는 다음과 같다. 1. 간(肝) 및 위장(胃臟)의 total lipid함량(含量)이 chloroform만 투여(投與)한 실험군(實驗群)에서는 그 투여(投與)농도가 높아질수록 증가하였으며, chloroform과 화분립(花粉粒)과 함께 투여(投與)한 군(群)에서는 chloroform의 투여(投與)농도가 낮을수록 대조군(對照群)과 비슷한 함량(含量)을 나타내었다. 2. total cholesterol함량(含量)은 혈청(血淸), 간(肝) 및 위장(胃臟) 모두 chloroform만 투여(投與)한 실험군(實驗群)에서는 대조군(對照群)보다 많았으나, 화분립(花粉粒)을 함께 투여(投與)함으로 그 함량(含量)이 감소하는 경향을 보였으며, I-P군(群)의 혈청(血淸)은 대조군(對照群)보다도 더 낮은 수치를 나타내기도 했다. 3. sGOT, sGPT 및 LDH 모두 chloroform의 투여(投與)농도 증가에 비해하여 활성(活性)의 증가를 나타냈으며, 화분립(花粉粒)을 함께 투여(投與)한 군(群)에서는 대조군(對照群)과 거의 비슷한 활성(活性)으로의 저하가 보였으나, Chloroform 투여(投與)농도가 클수록 활성(活性) 저하 정도는 감소하였다. 4. LDH isozyme의 Cellulose acetate electrophoresis결과(結果), 간(肝)에서는 choroform을 투여(投與)함으로 $LDH_5$의 증가가, 화분립(花粉粒)을 함께 투여(投與)함으로 $LDH_5$의 감소가 나타났으나 유의성(有意性)은 없었으며 위장(胃臟)에서도 isozyme 상호간의 유의적(有意鈴)인 변화(變化)는 나타나지 않았다.

• 한국식품영양학회지
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• 제7권2호
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• pp.108-113
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• 1994

This study was carried out to evaluate the effect of Cnidi rhizoma (CR) water extract on fat accumulation In fatted rats induced by the oral high fat administration for six weeks. To accomplish this evaluation, the serum and liver tissue have been examined for enzyme activity, cortisol and insulin level. The change of liver or tissue have been observed by the light microscope. GOT GPT and LDH activities were lower than the control group. Insulin and cortisol were higher than the control group, due to the fat accumulation. The liver of the control group observed by the tight microscope appeared to the fatty liver, but CR group showed some improvement of the fatty liver Based on the above results, it was shown that it is possible to improve fat accumulation induced by high fat dietary through using the oral administration of Cnidi rhizoma water extract.

• 한국식생활문화학회지
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• 제5권2호
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• pp.253-257
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• 1990

The effect of water extract in fruits of Omija (Schizandra chinensis Ballion) on liver function in expermental liver injuries induced by $CCl_4$. The levels of GPT, GOT activities in serum were decreased apparently on the dosage of water extract in fruits of Omija correspond to raw Omija 1g compared to control group. The levels of Microsomal protein, glycogen, and pyruvate in liver and of Urea nitrogen, GPT, GOT, LDH in serum showed a trend toward restoration of normalization. No effective of levels of hepatic G6P DH activity and serum free fatty acid by water extract in fruits of Omija.