• The Korean Journal of Physiology and Pharmacology
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• v.19 no.2
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• pp.141-149
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• 2015

"G protein-coupled receptor 40" (GPR40), a receptor for long-chain fatty acids, mediates the stimulation of glucose-induced insulin secretion. We examined the profiles of differential gene expression in GPR40-activated cells treated with linoleic acid, and finally predicted the integral pathways of the cellular mechanism of GPR40-mediated insulinotropic effects. After constructing a GPR40-overexpressing stable cell line (RIN-40) from the rat pancreatic ${\beta}$-cell line RIN-5f, we determined the gene expression profiles of RIN-5f and RIN-40. In total, 1004 genes, the expression of which was altered at least twofold, were selected in RIN-5f versus RIN-40. Moreover, the differential genetic profiles were investigated in RIN-40 cells treated with $30{\mu}M$ linoleic acid, which resulted in selection of 93 genes in RIN-40 versus RIN-40 treated with linoleic acid. Based on the Kyoto Encyclopedia of Genes and Genomes Pathway (KEGG, http://www.genome.jp/kegg/), sets of genes induced differentially by treatment with linoleic acid in RIN-40 cells were found to be related to mitogen-activated protein (MAP) kinase- and neuroactive ligand-receptor interaction pathways. A gene ontology (GO) study revealed that more than 30% of the genes were associated with signal transduction and cell proliferation. Thus, this study elucidated a gene expression pattern relevant to the signal pathways that are regulated by GPR40 activation during the acute period. Together, these findings increase our mechanistic understanding of endogenous molecules associated with GPR40 function, and provide information useful for identification of a target for the management of type 2 diabetes mellitus.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.40 no.2
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• pp.109-118
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• 2022

GPR (Ground Penetrating Radar), developed as a technology for geotechnical investigations such as sinkhole exploration, was used limitedly as a method to resolve undetectable lines in underground facility exploration. To improve the accuracy of underground facility data, the government made it possible to explore underground facilities using a non-metallic pipeline probe from July 2022. However, GPR has a problem in that the exploration rate is lowered in the soil with high moisture content, such as soft soil, such as clay layer, and there is a lot of variation in long-term accuracy. In this study, as a way to improve the accuracy of exploration considering the characteristics of GPR and the environment of underground facilities, we propose a GPR exploration method for underground facilities using permittivity constant correction and pattern analysis of GPR image data. Through this study, the accuracy of underground facility exploration and high reproducibility were derived as a result of field verification applying GPR frequency band and heterogeneous GPR.

• Journal of the Korean Association of Geographic Information Studies
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• v.24 no.3
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• pp.32-40
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• 2021

Underground information is difficult to visually check, which can lead to a huge accident in the event of a safety accident. Recently, the Ministry of Land, Infrastructure and Transport intends to reduce safety accidents caused by the aging or damage of underground facilities through the Special Act on Underground Safety Management. GPR is increasingly being used as a technology to acquire information in underground spaces that are difficult to see with the naked eye. However, GPR's location information is corrected by checking images of CCTV and GPS information acquired during exploration. This method has an average error of about 2 meters. In this works, We used LiDAR to calibrate the GPR information and found that the error was reduced from at least 7cm to up to 40cm. If accurate GPR information collected in the future is analyzed quickly using AI, etc., it will be able to collect and utilize underground information faster than it is now to secure safety.

• Biomolecules & Therapeutics
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• v.26 no.6
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• pp.599-607
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• 2018

Fasiglifam (TAK-875) a G-protein coupled receptor 40 (GPR40) agonist, significantly improves hyperglycemia without hypoglycemia and weight gain, the major side effects of conventional anti-diabetics. Unfortunately, during multi-center Phase 3 clinical trials, unexpected liver toxicity resulted in premature termination of its development. Here, we investigated whether TAK-875 directly inflicts toxicity on hepatocytes and explored its underlying mechanism of toxicity. TAK-875 decreased viability of 2D and 3D cultures of HepG2, a human hepatocarcinoma cell line, in concentration-(>$50{\mu}M$) and time-dependent manners, both of which corresponded with ROS generation. An antioxidant, N-acetylcysteine, attenuated TAK-875-mediated hepatotoxicity, which confirmed the role of ROS generation. Of note, knockdown of GPR40 using siRNA abolished the hepatotoxicity of TAK-875 and attenuated ROS generation. In contrast, TAK-875 induced no cytotoxicity in fibroblasts up to $500{\mu}M$. Supporting the hepatotoxic potential of TAK-875, exposure to TAK-875 resulted in increased mortality of zebrafish larvae at$25{\mu}M$. Histopathological examination of zebrafish exposed to TAK-875 revealed severe hepatotoxicity as manifested by degenerated hypertrophic hepatocytes with cytoplasmic vacuolation and acentric nuclei, confirming that TAK-875 may induce direct hepatotoxicity and that ROS generation may be involved in a GPR40-dependent manner.

• Journal of the Korea Society of Computer and Information
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• v.26 no.2
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• pp.27-37
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• 2021

In this paper, we propose Automatic detection system of underground pipe which automatically detects underground pipe to help experts. Actual location of underground pipe does not match with blueprint due to various factors such as ground changes over time, construction discrepancies, etc. So, various accidents occur during excavation or just by ageing. Locating underground utilities is done through GPR exploration to prevent these accidents but there are shortage of experts, because GPR data is enormous and takes long time to analyze. In this paper, To analyze 3D GPR data automatically, we use 3D image segmentation, one of deep learning technique, and propose proper data generation algorithm. We also propose data augmentation technique and pre-processing module that are adequate to GPR data. In experiment results, we found the possibility for pipe analysis using image segmentation through our system recorded the performance of F1 score 40.4%.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.40 no.3
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• pp.285-294
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• 2020

This study investigated the use of Ground Penetrating Radar (GPR) over a two-decade period on public roads, focusing on the electromagnetic characteristics of the pavement dielectrics and attenuation. From the results, a typical range of characteristic value, influencing factors, and a correction method were suggested. The typical dielectrics of asphalt pavements were 4-7, as measured by an air-coupled 1 GHz GPR antenna. The dielectrics of concrete pavements were very large in the early age, but were drastically reduced with ageing. Ten years on, collection was in the range of 6-12. The dielectrics were proportional to the relative humidity (R.H.) of the atmosphere. The effects were reduced to one eighth with an overlay. Attenuation generally increased with thickness of the road layer, and also increased where there was damage. The GPR results could also vary depending on the weather conditions as well as on the characteristics of the GPR equipment, even at the same frequency. Therefore, GPR surveys should be performed on road surfaces without debris on a single, fine day. The reliability of the GPR analysis could be improved by cores and equipment calibration with other non-destructive test surveys.

• Journal of the Korean earth science society
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• v.40 no.6
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• pp.561-571
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• 2019

To understand the genesis of tropical cyclones (TC), we computed TC genesis probability (GPr) by partitioning a highly localized genesis frequency (GFq) into nearby grid boxes in proportion to the spatial coherence of genesis potential index (GPI). From the analysis of TCs simulated by the Seoul National University Atmosphere Model Version 0 and the observed TCs, it was shown that GPr reasonably converges to GFq when averaged over a long-term period in a decent grid size, supporting its validity as a proxy representing a true TC GPr. The composite anomalies of the gridded GPr in association with the Asia summer monsoon, El Nino-Southern Oscillation (ENSO), and the Madden-Julian Oscillation (MJO) are much less noisy than those of GFq, and consequently are better interpretable. In summary, GPr converges to GFq, varies more smoothly than GFq, represents the spatiotemporal variations of GFq better than GPI, and depicts GFq with greater spatial details than other spatially smoothed GFqs.

• The Journal of Korean Society for Radiation Therapy
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• v.27 no.2
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• pp.167-174
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• 2015

Purpose : The pre-treatment QA using Portal dosimetry for Volumetric Arc Therapy To analyze whether maintaining the reproducibility depending on various factors. Materials and Methods : Test was used for TrueBeam STx$^{TM}$ (Ver.1.5, Varian, USA). Varian Eclipse Treatment planning system(TPS) was used for planning with total of seven patients include head and neck cancer, lung cancer, prostate cancer, and cervical cancer was established for a Portal dosimetry QA plan. In order to measure these plans, Portal Dosimetry application (Ver.10) (Varian) and Portal Vision aS1000 Imager was used. Each Points of QA was determined by dividing, before and after morning treatment, and the after afternoon treatment ended (after 4 hours). Calibration of EPID(Dark field correction, Flood field correction, Dose normalization) was implemented before Every QA measure points. MLC initialize was implemented after each QA points and QA was retried. Also before QA measurements, Beam Ouput at the each of QA points was measured using the Water Phantom and Ionization chamber(IBA dosimetry, Germany). Results : The mean values of the Gamma pass rate(GPR, 3%, 3mm) for every patients between morning, afternoon and evening was 97.3%, 96.1%, 95.4% and the patient's showing maximum difference was 95.7%, 94.2% 93.7%. The mean value of GPR before and after EPID calibration were 95.94%, 96.01%. The mean value of Beam Output were 100.45%, 100.46%, 100.59% at each QA points. The mean value of GPR before and after MLC initialization were 95.83%, 96.40%. Conclusion : Maintain the reproducibility of the Portal Dosimetry as a VMAT QA tool required management of the various factors that can affect the dosimetry.

• 한국지구물리탐사학회:학술대회논문집
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• 2004.08a
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• pp.180-189
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• 2004

The name of (Ban)wolseong thanks to its having a shape like a half-moon. Wolseong fortress is one of 'Gyeongju Historic Area', world heritage. The Silla kingdom's royal palace previously maintained its capital fortress at the locale of Wolseong. However its real face has been kept in mystery. This study grafts Geophysics on modern Archaeology and approaches with scientific and systematic methods to an excavation plan or archaeological study by means of GPR exploration which can complement archaeological curiosity without destroying Wolseong fortress. The exploration area is $12,000m^2$ in front of Seokbinggo(stone storage for ice). It is only $10\%$ area of Wolseong fortress. As a conclusion, GPR detected 7 of squared buildings($8{\times}8m^2$ size), stone wall, an entrance for the fortress, lots of foundation stones, road(presumptive), and a presumptive pond which size is 35${\~}$40m in diameter.

• Journal of the Society of Disaster Information
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• v.18 no.2
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• pp.261-268
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• 2022

Purpose: In the case of cavity discovered by ground penetrating radar exploration, it is necessary to accurately predict the filling amount in the cavity in advance, fill the cavity sufficiently and exert strength to ensure stability and prevent ground subsidence. Method: The cavity waveform analysis method by GPR exploration and the method using the cavity shape imaging equipment were performed to measure the cavity shape with irregular size and shape of the actual cavity, and the amount of cavity filling of the injection material was calculated during rapid restoration. Results: The expected filling amount was presented by analyzing the correlation between the cavity size and the filling amount of injection material according to the cavity scale and soil depth through the method by GPR exploration and the cavity scale calculation using the cavity shaping equipment. Conclusion: The cavity scale measured by the cavity imaging equipment was found to be in the range of 20% to 40% of the cavity scale by GPR exploration. In addition, the filling amount of injection material compared to the cavity scale predicted by GPR exploration was in the range of about 60% to 140%, and the filling amount of the injection material compared to the cavity size by the cavity shaping equipment was confirmed to be about 260% to 320%.