• Bulletin of the Korean Chemical Society
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• v.28 no.10
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• pp.1792-1796
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• 2007

Insulating effects on Au electrode according to the thickness and density of coated materials are well-known. To do electrochemical immunoassay reproducibly the glod electrode would be coated with self-assembled monolayers and antobodies. To get reproducibility, the antobody monolayer should be packed at highest density so that the amount of immobilized antibody at defined area should be the same. The calix[4]crown-5 SAMs could provide the basis for the antibodies to be immobilized reproducibly and at highest density. But the insulating effect would be highest too. We proved that the compactly packed protein monolayers on SAMs inhibited the electron transfer by block the free shuttling of redox molecules. The inhibition was minimized by inserting redox molecules in between the proteins during immobilization process. In this paper, we demonstrated that the calix[4]crown-5 SAMs would provide the protein monolayers with highest density and new method to minimize the insulating effect by inserted redox molecules in between the compactly packed protein monolayers.

• Journal of Microbiology and Biotechnology
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• v.16 no.11
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• pp.1778-1783
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• 2006

Event-specific PCR detection methods are the primary trend in genetically modified (GM) plant detection owing to their high specificity based on the flanking sequence of the exogenous integrant. Therefore, this study describes a real-time PCR system for event-specific GM canola GT73, consisting of a set of primers, TaqMan probe, and single target standard plasmid. For the specific detection of GT73 canola, the 3'-integration junction sequence between the host plant DNA and the integrated specific border was targeted. To validate the proposed method, test samples of 0, 1, 3, 5, and 10% GT73 canola were quantified. The method was also assayed with 15 different plants, and no amplification signal was observed in a real-time PCR assay with any of the species tested, other than GT73 canola.

• Journal of Aerospace System Engineering
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• v.10 no.3
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• pp.23-31
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• 2016

The intermediate mixing chamber is one of various methods for improving the regression rate and combustion efficiency of the hybrid rocket. The mixing chamber with its non-combustible material makes the propulsion performance increase, but it leads to a low fuel-loading density in the combustion chamber; therefore, this performance-related trade-off between the mixing chamber and the low fuel-loading density was studied. In this study, the trade-off was conducted by comparing the intermediate-mixing-chamber case with a w/o-mixing-chamber case. The small hybrid-sounding rocket is designed with internal ballistics for comparing the rocket length to the weight. In addition, an external ballistic analysis was conducted for comparing the performances of the w/- and w/o-mixing-chamber cases. As a result, the intermediate-mixing-chamber case shows that the length and the weight were decreased to 12 % and 8 %, respectively; furthermore, when compared with the normal cases, the estimated altitude result of the w/-mixing-chamber case was increased to approximately 75 m.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2009.05a
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• pp.237-240
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• 2009

To estimate the surface temperature for the hybrid solid fuels correctly is very important for the modelling of the hybrid combustion. Because it is used for the calculation of regression rate. In this study, The measurement of the surface temperature were performed with the solid fuels inserted the thermocouple. Its variation was investigated in the range of mass flux for an oxidizer.

• Journal of Electrochemical Science and Technology
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• v.2 no.2
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• pp.124-129
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• 2011

A simple process of fabricating micropillar structure and its influence upon enhancing electrochemical biosensor response were studied in this work. Conducting polymer PEDOT was used as a base material in formulating a composite with PVA. Micro porous PC membrane filter was used as a template for the micropillar of the composite on ITO electrode. This structure could provide plenty of encapsulating space for enzyme species. After dosing enzyme solution into this space, Nafion film tent was cast over the pillar structure to complete the micropillar cavity structure. In this way, the encapsulation of enzyme could be accomplished without any chemical modification. The amount of enzyme species was easily controllable by varying the concentration of the dosing solution. The more amount of enzyme is stored in the sensor, the higher the electrochemical response is produced. One more reason for the sensitivity improvement comes from the large surface area of the micropillar structure. Application of 0.7 V produced the best current response under the condition of pH 7.4. This biosensor showed linear response to the glucose in 0.1~1 mM range with the average sensitivity of $14.06{\mu}A/mMcm^2$. Detection limit was 0.01 mM based on S/N = 3.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.15 no.2
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• pp.141-146
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• 2002

We synthesized polypyrrole (PPy) by electrolysis of the pyrrole monomer solution containing support electrolyte, KCl and/or p-toluene sulfonic acid sodium salt (p-TS). The electrochemical behavior, was investigated using cyclic voltammetry and AC impedance. In the case of using electrolyte p-TS, the oxidation potential of the PPy was about -02 V vs Ag/AgCl reference electrode, while the potential was about 0 V for using electrolyte KCl. The falloff of the oxidation potential gave a sign of an improvement in the electron hopoing mechanism on the backbone. The AC impedance plot gave a hint of betterment of mass transport. PPy doped with p-TS improved in mass transport or diffusion. That was because the PPy doped with p-TS was more porous than PPy with KCl. We attained an effect of good kinetic parameters, in the case of PP-GOx enzyme electrodes doped with p-TS, which were determined by 58 mmol dm$\^$-3/ for apparent Michaelis constant and by 581 ㎂ for maximum current respectively.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2010.05a
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• pp.197-201
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• 2010

Precise control of oxidizer mass flow rate is important for hybrid rocket thrust control. In this study, oxidizer mass flow rate control system is developed by using stepping motor which is controlled by Labview program. Gox is used for oxidizer and PolyCarbonate, PolyEthylene, and PMMA is used for solid propellant. To suppress thrust oscillation during thrust control experiment, schematics of the experiment is analysised and revised. Results show that thrust oscillation is suppressed successfully.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2017.05a
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• pp.781-786
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• 2017

This study aims to acquire a basic clustering technology of hybrid rocket motor for lunar lander, including the oxidizer flow distribution characteristic and the simultaneous ignition characteristic. The experimental setups were established to conduct a series of ground firing test of a clustered motor. The gaseous oxygen (GOX) and the HDPE (High Density PolyEthylene) were used as the oxidizer and the solid fuel, respectively. Experimental results which are the simultaneous pyrotechnic ignition characteristic, the oxidizer distribution characteristic and the pressure traces of each combustion chamber imply that the hybrid rocket clustered motor works successfully.

• Journal of Information Processing Systems
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• v.14 no.4
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• pp.837-850
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• 2018

Bitcoin is a decentralized crypto-currency, which is based on the peer-to-peer network, and was introduced by Satoshi Nakamoto in 2008. Bitcoin transactions are written by using a scripting language. The hash value of a transaction's script is used to identify the transaction over the network. In February 2014, a Bitcoin exchange company, Mt. Gox, claimed that they had lost hundreds of millions US dollars worth of Bitcoins in an attack known as transaction malleability. Although known about since 2011, this was the first known attack that resulted in a company loosing multi-millions of US dollars in Bitcoins. Our reason for writing this paper is to understand Bitcoin transaction malleability and to propose an efficient solution. Our solution is a softfork (i.e., it can be gradually implemented). Towards the end of the paper we present a detailed analysis of our scheme with respect to various transaction malleability-based attack scenarios to show that our simple solution can prevent future incidents involving transaction malleability from occurring. We compare our scheme with existing approaches and present an analysis regarding the computational cost and storage requirements of our proposed solution, which shows the feasibility of our proposed scheme.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.52-55
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• 2000

Structually organized mono- and multilayers were developed on gold for the catalytic and affinity biosensing using hyper-branched dendrimers. For the catalytic biosensing interface, a new approach to construct a multilayered enzyme film on the electrode surface was developed. The film was prepared by layer-by-layer depositions of dendrimers and periodate-oxidized glucose oxidase. The voltammograms obtained from the GOx/dendrimer multilayered electrodes revealed that bioelectrocatalytic response is directly correlated to the number of deposited bilayers. From the analysis of voltammetric and ellipsometric signals, the coverage of active enzyme per layer during the layering steps was estimated, demonstrating the spatially-ordered multilayer formation. As an extension of the study, dendrimers having various degrees of ferrocenyl modification were prepared and used. The resulting electrodes were electrochemically characterized, and the density of ferrocenyl groups, active enzyme coverage, and sensitivity were estimated. For the affinity-sensing surrface, a biosensor system based on avidin-biotin interaction was developed. As the building block of affinity monolayer, G4 dendrimer having partial ferrocenyl-tethered surface groups was prepared and used. And the biotinylated and electroactive dendritic monolayer was used for the affinity-sensing surface interacting with avidin. Electrochemical characterization of the resulting biosensor was conducted using free enzyme in electrolyte in terms of degree of surface coverage with avidin and subsequent surface shielding.