• Korean Journal of Food Science and Technology
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• v.52 no.1
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• pp.40-45
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• 2020

Over 500 genetically modified organisms (GMOs) have been developed since 1996, of which nearly 44% have glufosinate herbicide-tolerant traits. Identification of specific markers that can be used to identify herbicide-tolerant traits is challenging as the DNA sequences of the gene(s) of a trait are highly variable depending on the origin of the gene(s), plant species, and developers. To develop specific PCR marker(s) for the detection of the glufosinate-tolerance trait, DNA sequences of several pat or bar genes were compared and a diverse combination of PCR primer sets were examined using certified reference materials or transgenic plants. Based on both the qualitative and quantitative PCR tests, a primer set specific for pat and non-specific for bar was developed. Additionally, a set of markers that can detect both pat and bar was developed, and the quantitative PCR data indicated that the primer pairs were sensitive enough to detect 0.1% of the mixed seed content rate.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.220-222
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• 2003

Legislation enacted worldwide to regulate the content of genetically modified organisms (GMOs) in crops, foods, and ingredients, reliable and sensitive methods for GMO detection have been developed. Proteins produced in GMO plants can be determined by qualitative and quantitative analyses and thus GMO designation has performed exactly. Target proteins selected in this study were neomycin phosphotransferase II (NPTII), 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS), cucumber mosaic virus(CMV), and phosphinothricin acetyltransferase (PAT). Analytical method employing western blotting was used for final characterization.