• Korean Journal of Food Science and Technology
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• v.42 no.2
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• pp.246-249
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• 2010

Diacylglycerol (DAG) was produced from lipase by the catalyzed synthesis of soybean oil (SBO) and glyceryl monooleate (GMO) with Lipozyme TLIM (Thermomyces lanuginosa). Effects of reaction time, molar ratio and enzyme road were studied. When 2:1, 1:1 and 1:2 (SBO:GMO) molar ratios with 20% Lipozyme TLIM were applied in a 1-hr reaction, the concentrations of DAG produced were 17.8, 20.0 and 20.4 g/100 g oil, respectively. Different amounts (2, 5, 10 and 20%) of Lipozyme TLIM were used at a 1:2 (SBO:GMO) molar ratio, and the concentrations of DAG produced in a 1-hr reaction were 10.8, 14.0, 16.9 and 20.4 g/100 g oil, respectively. During a 72-hr reaction, 10.8-22.7 g/100 g oil of DAG were produced under the reaction conditions in this study.

• Journal of Pharmaceutical Investigation
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• v.37 no.5
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• pp.311-314
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• 2007

Paclitaxel (PTX) is an antineoplastic agent approved for the treatment of ovarian and breast carcinomas. However, the use of paclitaxel as an anticancer drug is limited by its extremely poor water solubility (below $0.3\;{\mu}g/mL$). Furthermore, it has very low bioavailability when administered orally because paclitaxel is a substrate of P-glycoprotein (P-gp) efflux pump. In this study, buccal delivery of PTX was investigated as one of the alternatives for PTX delivery. Ethanol and glyceryl monooleate (GMO) were selected as permeation enhancing agents to increase solubility and permeation across buccal mucosa of PTX. At the different concentrations of ethanol solution ($30{\sim}70\;w/w\;%$), PTX permeation was studied, followed effects of GMO in the concentration range of $2.5{\sim}25%$ with ethanol vesicle. The transbuccal ability of PTX was evaluated in vitro using Franz diffusion cells mounted with rabbit buccal mucosa. As a result, incorporation of PTX into ethanol vesicle with GMO significantly enhanced the PTX permeation in rabbit buccal mucosa. Particularly, the mixtures of ethanol:water:GMO at the ratio of 50:47.5:2.5 showed the most excellent enhancing ability. The results showed a promising possibility for buccal delivery of PTX.

• Journal of Life Science
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• v.16 no.5
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• pp.806-811
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• 2006

The regulation of labelling criterion for genetically modified (GM) foods has been enforced since 2001 in Korea. Therefore, GM soybean (GMS) or GM maize (GMM) processed foods must be labeled as GMO derived. We surveyed to see whether this regulation is kept relevantly or not and the distributive statue of GM processed foods. Using the method of polymerase chain reaction (PCR) based on endogenous gene (Le1n, SSIIb), promoter gene (P35S), terminator gene (NOS) and transgenic gene (RRS, Bt11, Bt176, GA21, T25, Mon810), we detected GMS and GMM processed foods circulating at the market in Busan area. Out of total 100 samples, 38 items were showed to be contaminated with recombinant gene by qualitative PCR. Among 82 domestic and 18 imported items, 32 (39.0%) and 6 (33.3%) items were detected with GM ingredients respectively. Also among the 80 soybean and 20 maize processed foods, 23 (28.7%) and 15 (75.0%) foods were sensitive to detect GMS and GMM ingredients respectively. For the qualitative PCR positive foods, we chased identity preservation (IP) certificates. And we verified that the PCR positive crops were grown up, harvested and shipped separately from GMO but just mixed with GMO in the threshold of the non attentional contamination levels (3%). Thus we can not find out any regulation-violent case at all. The results of this study will help to keep the regulations of GM labelling and be informative to consumers who want to know the laboratory results of GMO testing.

• Korean Journal of Agricultural Science
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• v.47 no.2
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• pp.229-237
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• 2020

This study assessed the effects of a dietary non-genetically modified organism (non-GMO) source on growth performance and nutrient digestibility of grower-finisher pigs. The dietary treatments were 1) rice-soybean meal-based control diet and 2) rice and non-GMO soybean meal-based diet. In the experiment 1, 60 growing pigs (initial body weight [BW] = 23.76 ± 3.42 kg) were randomly assigned to 1 of 2 dietary treatments with 6 pigs·pen^-1 (5 replications) for 6 weeks. In experiment 2, 48 finishing pigs (initial BW = 64.31 ± 6.17 kg) were randomly assigned to 1 of 2 treatment groups with 4 pigs·pen^-1 (6 replications) for 6 weeks. Measurements were the average daily gain (ADG), average daily feed intake (ADFI), gain-to-feed ratio (G : F), and nutrient digestibility. The growth performance was measured at the beginning and end of each period. The apparent total tract digestibility (ATTD) was determined by chromium oxide as an indigestible marker during the last 7 days of each experiment. During the grower period, pigs fed the diet containing the non-GMO soybean meal had a higher (p < 0.05) ADFI than those fed the control diet; however, there were no differences between the dietary treatments in the ADG, G : F, and ATTD. Moreover, the dietary treatments did not affect the ATTD and growth performance of the finishing pigs. In conclusion, the inclusion of non-GMO soybean meal in the diet had no negative effects on the growth rate and nutrient digestibility, indicating that non-GMO soybean meal can be used in diet formulations with other feed ingredients and be a substitute for conventional soybean meal.

• Journal of Plant Biotechnology
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• v.48 no.4
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• pp.201-206
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• 2021

To ensure the safety of developing or importing genetically modified organisms (GMOs), Korea has enacted the "LMO Act." Accordingly, the safety of using GMOs as food or feed is evaluated in accordance with the concept of "substantial equivalence" proposed by OECD. The allergenicity of GMOs is assessed as a part of their safety evaluation. The methods of allergenicity assessment have been discussed by various international organizations, such as the OECD, FAO, and WHO. The main methods used for the allergenicity assessment of proteins newly expressed in GMOs include assessment of the physicochemical stability of these proteins, evaluation of their amino acid homology with existing allergenic proteins, and serum screening. In this study, we describe guidelines and related studies for the allergenicity assessment of GM crops.

• Korean Journal of Organic Agriculture
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• v.14 no.2
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• pp.139-157
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• 2006

Biotechnology has reached the level of giving birth to new forms of life and with this is a growing controversy in the conflict between science and ethics. Especially, GMOs are closely linked to the food products we consume and thus, the majority of the public shows a very sensitive reaction to the safety of GMO food products. Many perspectives arose surrounding the issues of safety on the human body and the ecology. This outlines diverse structural mechanisms to be set up to ensure safety such as risk assessment, risk management etc. Despite the precautionary principle guaranteed in many ways, the problem arises whether and how this principle can be taken in the safety assessment. GMOs due to its uniqueness do not end with just the possession of the technology involved but must also be considered with the prerequisite that they could be cultured again. Therefore the reinforcement of safety assessment system is necessary. That is, the reinforcement of risk assessment including field tests, the consideration of socio-economic effects, the coordinated system of relevant authorities, the development of technology for safety assessment.

• Journal of Nutrition and Health
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• v.36 no.10
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• pp.1030-1035
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• 2003

This study was conducted to evaluate the safety of herbicide-resistant rice, a genetically modified organism (GMO) developed by the Rural Development Administration, in Sprague-Dawley rats. The nutrient content of herbicide-resistant polished and brown cooked rice was compared with that of conventional Ilpum polished and brown cooked rice to assess composition equivalence. Compositional analysis was performed to measure proximates, fiber, and minerals before animal feeding. Growing male rats were fed one of the following four diets for six weeks: Ilpum polished cooked rice (IP) and Ilpum brown cooked rice (IB) as a non-GMO and herbicide-resistant polished cooked rice (GP) and brown cooked rice (GB) as a GMO. We checked clinical symptoms (anorexia, salivation, diarrhea, polyuria, anuria, fecal change) every day, food intake, change of body weight twice a week, and serum biochemistry and organ weights after 6 weeks of experimental feeding among the four groups. Nutrient content of the herbicide-resistant rice was similar to that of the non-transgenic control and was within the published range observed for non-transgenic rice. We could not find any significant difference in the above-mentioned items as the index to be checked in the animals fed the GMO. These results suggest that the nutrient content of genetically modified herbicide-resistant rice is compositionally equivalent to that of conventional Ilpum rice and that growing male rats fed herbicide-resistant rice are no different from those fed Ilpum rice, non-GMO for 6 weeks.

• Journal of the Korean Chemical Society
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• v.49 no.3
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• pp.255-260
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• 2005

• Proceedings of the Korean Society of Environment and Ecology Conference
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• 2009.04a
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• pp.99-101
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• 2009

• Feed Journal
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• v.2
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• pp.65-69
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• 2003