Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Production of Diacylglycerol from Lipase by the Catalyzed Reaction of Soybean Oil and Glyceryl Monooleate

Lipozyme TLIM을 효소적 반응촉매로 이용한 glyceryl monooleate로부터의 diacylglycerol의 합성

  • Jeon, Mi-Sun (Department of Food Science and Technology, Chungnam National University) ;
  • Lee, Cho-Rong (Department of Food Science and Technology, Chungnam National University) ;
  • Lee, Ki-Teak (Department of Food Science and Technology, Chungnam National University)
  • Received : 2009.07.29
  • Accepted : 2009.12.18
  • Published : 2010.04.30
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Abstract

Diacylglycerol (DAG) was produced from lipase by the catalyzed synthesis of soybean oil (SBO) and glyceryl monooleate (GMO) with Lipozyme TLIM (Thermomyces lanuginosa). Effects of reaction time, molar ratio and enzyme road were studied. When 2:1, 1:1 and 1:2 (SBO:GMO) molar ratios with 20% Lipozyme TLIM were applied in a 1-hr reaction, the concentrations of DAG produced were 17.8, 20.0 and 20.4 g/100 g oil, respectively. Different amounts (2, 5, 10 and 20%) of Lipozyme TLIM were used at a 1:2 (SBO:GMO) molar ratio, and the concentrations of DAG produced in a 1-hr reaction were 10.8, 14.0, 16.9 and 20.4 g/100 g oil, respectively. During a 72-hr reaction, 10.8-22.7 g/100 g oil of DAG were produced under the reaction conditions in this study.

Glycerol과 oleic ethyl ester와의 반응, 그리고 glycerol과 oleic acid와의 반응에서는 DAG가 생성되지 않았었지만 GMO와 SBO를 기질로 사용한 반응에서는 DAG가 생성됨을 확인하였다. 기질의 몰 비율에 따른 DAG 생성율을 비교하기 위하여 효소량을 기질 질량의 20%로 동일하게 설정하고 SBO와 GMO의 몰비율을 각각 2:1, 1:1과 1:2로 하여 반응한 결과, 1 hr 후 DAG의 함량이 최대로 도달 하였고 그 후에는 큰 차이를 보이지 않았다. 1hr에서 가장 큰 DAG 함량을 보인 몰비율은 1:1과 1:2(SBO:GMO)일 때로 각각 20.0, 20.4 g/100 g oil로 나타났고, 이 두 몰비율 중 TAG 함량이 낮았던 1:2 몰비율을 이용하여 DAG를 합성 하였다. 한편, 효소의 사용량에 따른 DAG 생성율을 비교하기 위하여 SBO와 GMO의 몰비율을 1:2로 동일하게 설정하고 효소량을 기질 질량의 2, 5, 10, 20%로 하여 반응한 결과, 1 hr의 DAG 함량은 각각 10.8, 14.0, 16.9, 20.4 g/100 g oil로 나타났다. 1 hr 이후의 DAG 생성량을 보면 효소량을 20% 사용한 경우, 72 hr까지 18.9-22.0 g/100 g oil로 비슷한 함량을 나타내었으나 효소량을 기질 질량의 2, 5, 10%를 사용하였을 경우, 1 hr 이후에도 DAG 함량이 증가하는 경향을 보였다.

Keywords

References

  1. Maki KC, Davidson MH, Tsushima R, Matsuo N, Tokimitsu I, Umporowicz DM, Dicklin MR, Foster GS, Ingram KA, Anderson BD, Frost SD, Bell M. Consumption of diacylglycerol oil as part of a reduced-energy diet enhances loss of body weight and fat in comparison with consumption of a triacylglycerol control oil. Am. J. Clin. Nutr. 76: 1230-1236 (2002)
  2. Nagao T, Watanabe H, Goto N, Onizawa K, Taguchi H, Matsuo N, Yasukawa T, Tsushima R, Shimasaki H, Itakura H. Dietary diacylglycerol suppresses accumulation of body fat compared to triacylglycerol in men in a double-blind controlled trial. J. Nutr. 130: 792-797 (2000)
  3. Saito S, Tomonobu K, Hase T, Tokimitsu I. Effects of diacylglycerol on postprandial energy expenditure and respiratory quotient in healthy subjects. Nutrition 22: 30-35 (2006) https://doi.org/10.1016/j.nut.2005.04.010
  4. Plou FJ, Barandiaran M, Calvo MV, Ballesteros A, Pastor E. High-yield production of mono- and di-oleylglycerol by lipasecatalyzed hydrolysis of triolein. Enzyme Microb. Technol. 18: 66-71 (1996) https://doi.org/10.1016/0141-0229(96)00054-3
  5. Castillo E, Dossat V, Marty A, Condoret JS, Combes D. The role of silica gel in lipase-catalyzed esterification reactions of highpolar substrates. J. Am. Oil Chem. Soc. 74: 77-85 (1997) https://doi.org/10.1007/s11746-997-0148-3
  6. Kwon SJ, Han JJ, Rhee JS. Production and in situ separation of mono- or diacylglycerol catalyzed by lipases in n-hexane. Enzyme Microb. Technol. 17: 700-704 (1995) https://doi.org/10.1016/0141-0229(94)00117-A
  7. Kristensen JB, Xu X, Mu H. Diacylglycerol synthesis by enzymatic glycerolysis: Screening of commercially available lipases. J. Am. Oil Chem. Soc. 82: 329-334 (2005) https://doi.org/10.1007/s11746-005-1074-5
  8. Park RK, Lee KT. Synthesis and characterization of mono- and diacylglycerol enriched functional oil by enzymatic glycerolysis of corn oil. Korean J. Food Sci. Technol. 36: 211-216 (2004)