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Spectrophotometric evaluation of restorative composite shades and their match with a classical shade guide

  • Rafael Melara;Luciana Mendonca;Fabio Herrmann Coelho-de-Souza;Juliana Nunes Rolla;Luciano de Souza Goncalves
    • Restorative Dentistry and Endodontics
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    • v.46 no.4
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    • pp.60.1-60.8
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    • 2021
  • Objectives: The aim of this study was to verify the match between 5 shades of composites from different manufacturers with a shade guide and among the systems using a portable spectrophotometer. Materials and Methods: Shade measurements were performed on specimens of Z350 XT (3M ESPE), Charisma Diamond (Heraeus Kulzer GmbH), Esthet X-HD (Dentsply Caulk), and Empress Direct (Ivoclar-Vivadent) for shades A1, A2, A3, B1, and C3 using a Vita Easyshade spectrophotometer (Vita Zahnfabrik) against a white background. Corresponding shades of Vitapan Classical (Vita Zahnfabrik) guide were measured likewise and shade variation (ΔE) was calculated based on International Commission on Illumination L*a*b* parameters. The ΔE of the composites in each shade was compared by one-way analysis of variance and Tukey's post hoc test (α = 0.05). Results: All composites presented ΔE > 3.7 compared with the shade guide. Variation in shades A3, B1, and C3 was significantly different for all composites. ΔE of Z350 XT was significantly lower for A1 than for the other shades, whereas ΔE of Z350 XT and Charisma Diamond were significantly lower for A2 than for the other shades. Conclusions: No composite shade matched with the shade guide. Equivalent shades of the restorative composite from different manufacturers may show clinically noticeable ΔE.

Supplementation of guanidinoacetic acid and rumen-protected methionine increased growth performance and meat quality of Tan lambs

  • Zhang, Jian Hao;Li, Hai Hai;Zhang, Gui Jie;Zhang, Ying Hui;Liu, Bo;Huang, Shuai;Guyader, Jessie;Zhong, Rong Zhen
    • Animal Bioscience
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    • v.35 no.10
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    • pp.1556-1565
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    • 2022
  • Objective: Tan lambs (n = 36, 3 mo old, 19.1±0.53 kg) were used to assess effects of dietary guanidinoacetic acid (GAA) and rumen-protected methionine (RPM) on growth performance, carcass traits, meat quality, and serum parameters. Methods: Lambs were randomly assigned to three treatment groups, with 6 pens per group and 2 lambs per pen. Dietary treatments were: basal diet alone (I); basal diet supplemented with 0.08% GAA+0.06% RPM (II); and basal diet supplemented with 0.08% GAA+0.08% RPM (III). Diets were provided three times a day for 90 d. Intake per pen was recorded daily and individual lamb body weight (BW) was measured monthly. Carcass traits were measured after slaughter and meat quality at the end of the experiment, blood samples were taken on a subgroup of lambs for analysis of indicators mostly related to protein metabolism. Results: Final BW and average daily gain for the first and second month, and for the entire experiment were greater in Treatment II compared to Treatment I (p<0.05), whereas feed to gain ratio was lower (p<0.05). Treatment II had the optimal dressing percentage and net meat weight proportion, as well as crude protein and intramuscular fat concentrations in muscles. Treatment II improved meat quality, as indicated by the greater water holding capacity, pH after 45 min and 48 h, and lower shear force and cooking loss. Dietary supplementation of GAA and RPM also increased the meat color a* and b* values at 24 h. Finally, Treatment II increased total protein, and serum concentrations of albumin and creatinine, but decreased serum urea nitrogen concentrations, indicating improved protein efficiency. Conclusion: In this study, 0.08% GAA+0.06% RPM supplementation improved growth performance and meat quality of Tan lambs.

Morphological Characteristics and Germination Conditions of Seeds in Chamaedaphne calyculata (진퍼리꽃나무(Chamaedaphne calyculata) 종자의 형태적 특성 및 발아 조건)

  • Seung Ju Jo;Young Min Choi;Jung Won Sung;Jung Won Yoon
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2023.04a
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    • pp.24-24
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    • 2023
  • 본 실험은 한반도 극지 고산식물인 진퍼리꽃나무[Chamaedaphne calyculata (L.) Moench] 종자의 형태적 특성을 조사하고 변온조건과 저온처리 및 GA3 처리가 발아에 미치는 영향을 구명하고자 수행하였다. 진퍼리꽃나무 종자는 국립수목원 전시원에서 채종 후 4℃에 저온건조 저장하였으며 전자현미경(DVM6, Leica Microsystems GmbH, Wetzlar, Germany)을 이용하여 종자와 배의 길이를 측정하였다. 2022년 3월에 growth chamber 내에서 32일간 15/6℃, 25/15℃ 변온조건하에 배양하였으며, 저온층적처리(0, 40일) 및 GA3 처리(0/500/1,000mg·L-1) 처리에 따른 발아 특성을 구명하였다. 진퍼리꽃나무 종자의 최종발아율(FGP)은 25/15℃ GA3 1000mg·L-1 처리에서 42.2%로 가장 높게 조사되었다. 평균발아일수(MGT)는 15/6℃ 저온층적 처리에서 22.3일로 가장 높았으며, 대부분 15/6℃ 변온조건에서 25/15℃ 변온조건보다 6∼8일 높았다. 최종발아일의 평균은 약 12일로 확인되었다. 저온 층적처리 결과, 최종발아율(FGP)은 온도조건 25/15℃, 15/6℃에서 각각 32.2%와 11.1%를 보여 무처리구에서 조사된 24.4%와 10.0%보다 각각 7.8%, 1.1% 높게 나타났다. 호르몬처리 결과, 동일한 발아조건에서 GA3 500mg·L-1 처리보다 1000mg·L-1 처리에서 더 높은 발아율을 보였다. GA3 1000mg·L-1 처리에서의 최종발아율(FGP)DKSH은 25/15℃, 15/6℃ 각각 42.2%와 30.0%를 보여 무처리구 보다 각각 17.8%, 20% 높게 나타났다.

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Comparison of Foot Pressure Distribution During Single-leg Squat in Individuals With and Without Pronated Foot

  • Il-kyu Ahn;Gyeong-tae Gwak;Ui-jae Hwang;Hwa-ik Yoo;Oh-yun Kwon
    • Physical Therapy Korea
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    • v.31 no.1
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    • pp.40-47
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    • 2024
  • Background: Single-leg squat (SLS)s are commonly used as assessment tool and closed kinetic exercises are useful for assessing performance of the lower extremities. Pronated feet are associated with foot pressure distribution (FPD) during daily activities. Objects: To compare the FPD during SLSs between groups with pronated and normal feet. Methods: This cross-sectional study included 30 participants (15 each in the pronated foot and control groups) are recruited in this study. The foot posture index was used to distinguish between the pronated foot and control groups. The Zebris FDM (Zebris Medical GmbH) stance analysis system was used to measure the FPD on the dominant side during a SLS, which was divided into three phases. A two-way mixed-model ANOVA was used to identify significant differences in FPD between and within the two groups. Results: In the hallux, the results of the two-way mixed-model ANOVAs revealed a significant difference between the group and across different phases (p < 0.05). The hallux, and central forefoot were significantly different between the group (p < 0.05). Moreover, significant differences across different phases were observed in the hallux, medial forefoot, central forefoot, lateral forefoot, and rearfoot (p < 0.05). The post hoc t-tests were conducted for the hallux and forefoot central regions. In participants with pronated foot, the mean pressure was significantly greater in hallux and significantly lower, in the central forefoot during the descent and holding phases. Conclusion: SLSs are widely used as screening tests and exercises. These findings suggest that individuals with pronated feet should be cautious to avoid excessive pressure on the hallux during the descent-to-hold phase of a SLS.

Accuracy and time efficiency of conventional and digital outlining of extensions of denture foundation on preliminary casts

  • Anne Kaline Claudino Ribeiro;Aretha Heitor Verissimo;Rodrigo Falcao Carvalho Porto de Freitas;Rayanna Thayse Florencio Costa;Burak Yilmaz;Sandra Lucia Dantas de Moraes;Adriana da Fonte Porto Carreiro
    • The Journal of Advanced Prosthodontics
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    • v.16 no.3
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    • pp.139-150
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    • 2024
  • PURPOSE. The purpose of this diagnostic study was to assess the accuracy and time efficiency of a digital method to draw the denture foundation extension outline on preliminary casts compared with the conventional technique. MATERIALS AND METHODS. A total of 28 preliminary edentulous casts with no anatomical landmarks were digitized using a laboratory scanner. The outlining of the entire basal seat of the denture was performed on preliminary casts and digitized. Casts with no extension outline were digitized and outlines were drawn using software (DWOS, Straumann). The accuracy of the extension outlined between both techniques was evaluated in the software (GOM Inspect; GOM GmbH) by file superimposition. Specificity and sensitivity tests were applied to measure accuracy. The paired t-test (95% CI) was used to compare the mean total area and the working time. RESULTS. The accuracy ranged from 0.57 to 0.92. The buccal and labial frenulum showed a lower value in the maxilla (0.57); while the area between the retromolar pad and buccal frenulum (0.64) showed a lower score in the mandible. The maxillary denture foundation and the working time for both arches were significantly longer for the digital method (P < .001). CONCLUSION. The denture foundation extension outline exhibited a sufficiently excellent accuracy for the digital method, except for the maxillary anterior region. However, the digital method required a longer working time.

The TANDEM Euratom project: Context, objectives and workplan

  • C. Vaglio-Gaudard;M.T. Dominguez Bautista;M. Frignani;M. Futterer;A. Goicea;E. Hanus;T. Hollands;C. Lombardo;S. Lorenzi;J. Miss;G. Pavel;A. Pucciarelli;M. Ricotti;A. Ruby;C. Schneidesch;S. Sholomitsky;G. Simonini;V. Tulkki;K. Varri;L. Zezula;N. Wessberg
    • Nuclear Engineering and Technology
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    • v.56 no.3
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    • pp.993-1001
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    • 2024
  • The TANDEM project is a European initiative funded under the EURATOM program. The project started on September 2022 and has a duration of 36 months. TANDEM stands for Small Modular ReacTor for a European sAfe aNd Decarbonized Energy Mix. Small Modular Reactors (SMRs) can be hybridized with other energy sources, storage systems and energy conversion applications to provide electricity, heat and hydrogen. Hybrid energy systems have the potential to strongly contribute to the energy decarbonization targeting carbon-neutrality in Europe by 2050. However, the integration of nuclear reactors, particularly SMRs, in hybrid energy systems, is a new R&D topic to be investigated. In this context, the TANDEM project aims to develop assessments and tools to facilitate the safe and efficient integration of SMRs into low-carbon hybrid energy systems. An open-source "TANDEM" model library of hybrid system components will be developed in Modelica language which, by coupling, will extend the capabilities of existing tools implemented in the project. The project proposes to specifically address the safety issues of SMRs related to their integration into hybrid energy systems, involving specific interactions between SMRs and the rest of the hybrid systems; new initiating events may have to be considered in the safety approach. TANDEM will study two hybrid systems covering the main trends of the European energy policy and market evolution at 2035's horizon: a district heating network and power supply in a large urban area, and an energy hub serving energy conversion systems, including hydrogen production; the energy hub is inspired from a harbor-like infrastructure. TANDEM will provide assessments on SMR safety, hybrid system operationality and techno-economics. Societal considerations will also be encased by analyzing European citizen engagement in SMR technology safety.

Advanced Abdominal MRI Techniques and Problem-Solving Strategies (복부 자기공명영상 고급 기법과 문제 해결 전략)

  • Yoonhee Lee;Sungjin Yoon;So Hyun Park;Marcel Dominik Nickel
    • Journal of the Korean Society of Radiology
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    • v.85 no.2
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    • pp.345-362
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    • 2024
  • MRI plays an important role in abdominal imaging because of its ability to detect and characterize focal lesions. However, MRI examinations have several challenges, such as comparatively long scan times and motion management through breath-holding maneuvers. Techniques for reducing scan time with acceptable image quality, such as parallel imaging, compressed sensing, and cutting-edge deep learning techniques, have been developed to enable problem-solving strategies. Additionally, free-breathing techniques for dynamic contrast-enhanced imaging, such as extra-dimensional-volumetric interpolated breath-hold examination, golden-angle radial sparse parallel, and liver acceleration volume acquisition Star, can help patients with severe dyspnea or those under sedation to undergo abdominal MRI. We aimed to present various advanced abdominal MRI techniques for reducing the scan time while maintaining image quality and free-breathing techniques for dynamic imaging and illustrate cases using the techniques mentioned above. A review of these advanced techniques can assist in the appropriate interpretation of sequences.

Fracture resistance and marginal fidelity of zirconia crown according to the coping design and the cement type (코핑 디자인과 시멘트에 따른 지르코니아 도재관의 파절 저항성)

  • Sim, Hun-Bo;Kim, Yu-Jin;Kim, Min-Jeong;Shin, Mee-Ran;Oh, Sang-Chun
    • The Journal of Korean Academy of Prosthodontics
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    • v.48 no.3
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    • pp.194-201
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    • 2010
  • Purpose: The purpose was to compare the marginal fidelity and the fracture resistance of the zirconia crowns according to the various coping designs with different thicknesses and cement types. Materials and methods: Zirconia copings were designed and fabricated with various thicknesses using the CAD/CAM system (Everest, KaVo Dental GmbH, Biberach., Germany). Eighty zirconia copings were divided into 4 groups (Group I: even 0.3 mm thickness, Group II: 0.3 mm thickness on the buccal surface and the buccal half of occlusal surface and the 0.6 mm thickness on the lingual surface and the lingual half of occlusal surface, Group III: even 0.6 mm thickness, Group IV: 0.6 mm thickness on the buccal surface and the buccal half of occlusal surface and the 1.0 mm thickness on the lingual surface and the lingual half of occlusal surface) of 20. By using a putty index, zirconia crowns with the same size and contour were fabricated. Each group was divided into two subgroups by type of cement: Cavitec$^{(R)}$ (Kerr Co, USA) and Panavia-$F^{(R)}$ (Kuraray Medical Inc, Japan). After the cementation of the crowns with a static load compressor, the marginal fidelity of the zirconia crowns were measured at margins on the buccal, lingual, mesial and distal surfaces, using a microscope of microhardness tester (Matsuzawa, MXT-70, Japan, ${\times}100$). The fracture resistance of each crown was measured using a universal testing machine (Z020, Zwick, Germany) at a crosshead speed of 1 mm/min. The results were analyzed statistically by the two-way ANOVA and oneway ANOVA and Duncan's multiple range test at $\alpha$=.05. Results: Group I and III showed the smallest marginal fidelity, while group II demonstrated the largest value in Cavitec$^{(R)}$ subgroup (P<.05). For fracture resistance, group III and IV were significantly higher than group I and II in Cavitec$^{(R)}$ subgroup (P<.05). The fracture resistances of Panavia-$F^{(R)}$ subgroup were not significantly different among the groups (P>.05). Panavia-$F^{(R)}$ subgroup showed significantly higher fracture resistance than Cavitec$^{(R)}$ subgroup in group I and II (P<.05). Conclusion: Within the limitation of this study, considering fracture resistance or marginal fidelity and esthetics, a functional ceramic substructure design of the coping with slim visible surface can be used for esthetic purposes, or a thick invisible surface to support the veneering ceramic can be used depending on the priority.

Fluorine-releasing of Dental Restoration Materials in which the Fluorine is Contained (불소 유리로 본 불소 함유 수복재)

  • Kim, Joo-Won
    • The Journal of the Korea Contents Association
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    • v.12 no.5
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    • pp.311-322
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    • 2012
  • Restoration materials used to investigate effects of fluorine such as enamel strengthening and anti-caries effects in several types of dental restoration materials were five kinds including Ionoseal(VOCO GmbH, Cuxhaven, Germany), Fuji Filling LC(GC Co. Tokyo, Japan), Quadrant Universal LC(CAVEX Holland BV, Netherlands), PermaCem$^{(R)}$(DMG, Hamburg, Germany) and Dyract$^{(R)}$ AP(Dentsply GmbH, Germany), and the amount of fluorine-releasing was measured with ICS-5000 Reagent-FreeTM Ion Chromatography(RFICTM, Dionex, U.S.A.). The results of this study are as follows. 1. In all types of restoration materials, the amount of fluoride-releasing was reduced with time passage and it was declined sharply to show significance in four weeks. Fuji Filling LC(12.445PPM) or resin-reinforced glass ionomer and PermaCem$^{(R)}$(16.121PPM) or compomer were found to release fluorine for a long term(P<.001). 2. Ionoseal(0.887PPM) or glass ionomer and Quadrant Universal LC(0.957PPM) or composite resin released a few fluorine of 1PPM or less than 1PPM after one week, and Dyract$^{(R)}$ AP or compomer released fluorine of 8.631PPM in one week and its amount of releasing decreased dramatically in two and four week by recording 0.175PPM and 0.116PPM, respectively. Therefore, the effect of releasing fluorine in four weeks was observed to be poor (P<.001). 3. Fuji Filling LC or resin-reinforced glass ionomer and PermaCem$^{(R)}$ or compomer released fluorine of 33.372 and 1.902PPM, respectively in one week and their amount of releasing increased to be 36.371 and 18.223PPM, respectively in two weeks. So, their amount of fluorine-releasing recorded the highest levels in two weeks(P<.001).

Cloning of a Glutathione S-Transferase Decreasing During Differentiation of HL60 Cell Line (HL6O 세포주의 분화 시 감소 특성을 보이는 Glutathione S-Transferase의 클로닝)

  • Kim Jae Chul;Park In Kyu;Lee Kyu Bo;Sohn Sang Kyun;Kim Moo Kyu;Kim Jung Chul
    • Radiation Oncology Journal
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    • v.17 no.2
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    • pp.151-157
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    • 1999
  • Purpose : By sequencing the Erpressed Sequence Tags of human 걸ermal papilla CDNA library, we identified a clone named K872 of which the expression decreased during differentiation of HL6O cell line. Materials and Methods : K872 plasmid DNA was isolated according to QIA plasmid extraction kit (Qiagen GmbH, Germany). The nucleotide sequencing was performed by Sanger's method with K872 plasmid DNA. The most updated GenBank EMBL necleic acid banks were searched through the internet by using BLAST (Basic Local Alignment Search Tools) program. Nothern bots were performed using RNA isolated from various human tissues and cancer cell lines. The gene expression of the fusion protein was achieved by His-Patch Thiofusicn expression system and the protein product was identified on SDS-PAGE. Results : K872 clone is 1006 nucleotides long, and has a coding region of 675 nucleotides and a 3' non-coding region of 280 nucleotides. The presumed open reading frame starting at the 5' terminus of K872 encodes 226 amino acids, including the initiation methionine residue. The amino acid sequence deduced from the open reading frame of K872 shares $70\%$, identity with that of rat glutathione 5-transferase kappa 1 (rGSTKl). The transcripts were expressed in a variety of human tissues and cancer cells. The levels of transcript were relatively high in those tissues such as heart, skeletal muscle, and peripheral blood leukocyte. It is noteworthy that K872 was found to be abundantly expressed in coloreetal cancer and melanoma cell lines. Conclusion : Homology search result suggests that K872 clone is the human homolog of the rGSTK1 which is known to be involved in the resistance of cytotoxic therapy. We propose that meticulous functional analysis should be followed to confirm that.

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