• Journal of Life Science
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• v.30 no.5
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• pp.411-419
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• 2020

The development of GM crops has gained significant economic importance, and the number of countries cultivating commercial GM crops has continuously increased since the 1960s. Globally, the area given to cultivating GM soybean, maize, cotton, and canola alone had reached 114 million hectares by 2007. Although the economic importance of cultivating and commercializing GM crops has increased, there is still a need to assess their agricultural traits in comparison to non-GM produce. This study evaluated the agricultural traits of GM rice containing the drought-tolerant gene CaMsrB2 and standard rice to investigate any unintended effects of genetic engineering. The GM and non-GM rice were compared in terms of various agricultural traits in a drought greenhouse and an irrigated paddy field. There was no statistical difference in the field-grown crops, but there was a statistically significant difference in both tiller number and yield in the greenhouse. These results therefore suggest that GM rice lines containing the CaMsrB2 gene are superior in performance to non-GM rice in drought stress conditions and could be grown in drought-prone areas where drought intolerant rice may not be able to grow.

• Korean Journal of Food Science and Technology
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• v.36 no.5
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• pp.828-832
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• 2004

Enzyme-linked immune sorbent assay (ELISA) was applied to quantify soy protein in fresh soybean curd (bean curd) produced by combination of genetically modified (GM) and genetically not modified (non-GM) soybeans. Antibodies against 113 and 24 kDa proteins, which appeared only in non-GM bean curd (specific band), and in both non-GM and GM bean curds (non-specific band) based on SDS-PAGE results, were prepared by immunization to rabbit. Through ELISA using either antibody, GM bean curd protein content was determined at dilution rates of $10^{-1}-10^{-6}$. Standard curve showing relationship between ELISA optical density and non-GM protein content was produced using antibody against 113 kDa protein at protein dilution between $10^{-7}\;to\;10^{-6}$, highly antigen content-dependent dilution. Bean curd prepared by random combinations of GM and non-GM soybeans were analyzed by ELISA, and standard curve was produced. Results reveal non-GM protein content of bean curd could be quantified with higher than 93% accuracy.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2022.06a
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• pp.419-421
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• 2022

어선은 어획물로 인한 무게 변화 및 어구 사용에 의한 외력으로 GM이 변화한다. 하지만 실시간으로 변화하는 GM을 추정하는 것은 어려우며, 현재 적용되는 추정식은 어선에 대입하기에 정확성이 떨어진다. 이에 본 논문은 어선의 GM 추정식 개발에 기여하기 위해 어선의 Roll 운동응답을 분석하고, 실험과 시뮬레이션의 GM과 파경사 관계를 밝히고자 한다. 7톤 급의 실제 운항 중인 어선의 모델선 GM을 변화시키면서 파경사별 Roll 운동응답 데이터를 수집한다. GM 변화의 경우 선박 무게중심부에 높이 방향으로 추를 조절할 수 있도록 했으며, 파경사는 동일 주기 4가지 파경사를 선정하였다. GM과 파경사가 커질수록 Roll 응답이 증가하는 경향을 보이며, GM과 파경사가 커짐에 따라 기울기가 비선형성을 보이며 증가하는 것을 확인하였다. 본 연구를 통해서 어선의 GM과 파경사의 관계를 파악하고, GM추정에 사용할 수 있느 보정 계수를 도출할 수 있다. 추후에 본 연구 내용을 활용하여 운항 중인 어선의 실시간 GM 추정시스템 개발에 기여가 가능할 것으로 생각한다.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.100-100
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• 2017

FT is one of the major floral activator in plant flowering. FT determines the changing point of time from vegetative stage to reproductive stage. To understand the role of FT homologs in short-day plant soybean, we identified 10 soybean FT homologous genes and named GmFTs. We figured out that 10 GmFT genes were further categorized into three subclades through phylogenetic analysis. Expression analysis of GmFT genes indicated that they might have different functions in photoperiod-dependent soybean flowering. Most of GmFTs, for example, GmFT2a, GmFT2b, GmFT5a and GmFT6 mainly expressed in soybean leaves at short-day condition. However, interestingly GmFT1a and GmFT4 represented opposite expression pattern to other GmFTs. Arabidopsis transgenic plants overexpressing GmFT2a and GmFT5a exhibited extremely early flowering. In contrast, overexpression of GmFT4 delayed flowering of Arabidopsis transgenic plants. The results suggest that GmFT4 has antagonistic role to other GmFTs in soybean flowering. Interestingly, mRNA level of GmFT2a is higher in early flowering soybean accessions than in late flowering ones. Moreover, the highest point of mRNA level of GmFT2a showed the positive correlation with the timing of flowering of soybean accessions. But that of GmFT4 showed opposite pattern. Here, we report that soybean FT homologs might acquire different functions in photoperiod-dependent flowering through the functional diversification during evolution.

• Journal of Life Science
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• v.22 no.9
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• pp.1173-1179
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• 2012

The importance of genetic stability and bio-safety in the environment has recently been recognized for many (genetically modified) GM plants. This study evaluated the GM safety of transgenic rice and its environmental variance. Data on agronomic characters and principal component were collected for vitamin A-enriched GM rice and four check cultivars in a large GM field trial during 2009-2011. The cultivation environment was a large GM field and a greenhouse. In this experiment, there was no significant difference between the agronomic characters of the GM rice and those of a donor plant, 'Nagdong'. In terms of grain characteristics, the appearance and physicochemical characteristics of the GM rice and those of the donor plant were similar. However, the grain of the GM rice developed a white core and a white belly when planted in the greenhouse. The type and distribution of dominant weed species were not different in the GM rice and the 'Nagdong'. In addition, gene flow was not detected in the dominant weed species based on PCR analysis.

• Molecules and Cells
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• v.39 no.10
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• pp.734-741
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• 2016

Granulocyte-macrophage colony stimulating factor (GM-CSF) has a role in inducing emergency hematopoiesis upon exposure to inflammatory stimuli. Although GM-CSF generated murine bone marrow derived cells have been widely used as macrophages or dendritic cells in research, the exact characteristics of each cell population have not yet been defined. Here we discriminated GM-CSF grown bone marrow derived macrophages (GM-BMMs) from dendritic cells (GM-BMDCs) in several criteria. After C57BL/6J mice bone marrow cell culture for 7 days with GM-CSF supplementation, two main populations were observed in the attached cells based on MHCII and F4/80 marker expressions. GM-BMMs had $MHCII^{low}F4/80^{high}$ as well as $CD11c^+CD11b^{high}CD80^-CD64^+MerTK^+$ phenotypes. In contrast, GM-BMDCs had $MHCII^{high}F4/80^{low}$ and $CD11c^{high}CD8{\alpha}^-CD11b^+CD80^+CD64^-MerTK^{low}$ phenotypes. Interestingly, the GM-BMM population increased but GM-BMDCs decreased in a GM-CSF dose-dependent manner. Functionally, GM-BMMs showed extremely high phagocytic abilities and produced higher IL-10 upon LPS stimulation. GM-BMDCs, however, could not phagocytose as well, but were efficient at producing $TNF{\alpha}$, $IL-1{\beta}$, IL-12p70 and IL-6 as well as inducing T cell proliferation. Finally, whole transcriptome analysis revealed that GM-BMMs and GM-BMDCs are overlap with in vivo resident macrophages and dendritic cells, respectively. Taken together, our study shows the heterogeneicity of GM-CSF derived cell populations, and specifically characterizes GM-CSF derived macrophages compared to dendritic cells.

• Journal of Families and Better Life
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• v.21 no.2
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• pp.19-30
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• 2003

In this study, housewives' attitudes toward genetically modified foods (GM foods) and their willingness to purchase GM foods were examined. The findings of this study could provide useful information for consumer education and consumer policy development regarding GM foods. The specific purposes of this study were: (1) to examine consumers' attitudes toward GM foods, (2) to analyze the effect of the perception of GM foods and demographic variables on consumers' attitudes toward GM foods, and (3) to analyze the effect of the perception of GM foods and demographic variables on consumers' willingness to purchase GM foods. The questionnaire used in the survey was constructed by the author, based on existing literature. The survey was conducted with 1,100 housewives, and 723 of the completed survey forms were used in the final analysis. Frequencies, percentages, means, standard deviation, t-tests, ANOVA, Duncan-test, Pearson's Correlation, factor analysis, and discriminant analysis were employed for data analysis methods. Major findings are: (1) Consumers' attitudes toward GM foods consist of three factors, that are, attitude regarding potential danger, attitude regarding the use of GM technique on plants, and attitude regarding the use of GM technique on animals. (2) Consumers with a higher level of education tend to perceive GM food as more dangerous, whereas consumers with a lower level of education tend to accept more the use of GM technique on plants. (3) Consumers who tend not to consider GM foods as dangerous, and those who acknowledged benefits in using GM technique on plants are more willing to buy GM foods.

• Journal of Korea Trade
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• v.23 no.6
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• pp.131-144
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• 2019

Purpose - The purpose of this study was to investigate market power of soybeans exported by the United States to Korea. Particularly, this paper considered dichotomous characteristics of genetically modified (GM) soybeans and non-GM soybeans and conducted empirical analysis of these two segregated soybean markets to understand key tenets of market power in international soybean trade. Design/methodology - The difference in market power between GM and non-GM soybeans was analyzed using Residual Demand Elasticity (RDE) and Residual Supply Elasticity (RSE) models over the period of 2008~2018. RDE and RSE models under an imperfect competition condition were used to estimate market margins and determine whether GM and non-GM exporters or importers exercised market power in the destination market. Findings - Empirical results suggested that the U.S. had a market power on both GM and non-GM soybean exports. GM exports had greater market power than non-GM exports (14% vs. 9%). By contrast, Korea showed an inability to grab market margin or exert market power in soybean imports. Both export supply by the U.S. and import demand by Korea were found to be more responsive to price changes of GM soybeans than to prices changes of non-GM soybeans. This might be due to a self-interested, profit-seeking strategy by the exporter and many concerned consumers regarding potential adverse effects of GMOs in the importing country. Originality/value - This paper fills the literature gap by exploiting market power in both GM and non-GM markets with explicit consideration of price correlations between GM and non-GM soybeans in Korea. A number of existing studies have provided evidence for market power broadly embedded in international commodity trade. However, studies focusing on Korean markets are limited. No study has explored the country's soybean trade. Furthermore, the majority of prior studies have almost exclusively focused on the market power from a standpoint of exporting countries without discussing importers' market structure. This paper also sought to understand potentially distinguished patterns of market power between GM and non-GM markets.

• KSBB Journal
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• v.16 no.4
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• pp.376-380
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• 2001

The human granulocyte-macrophage colony-stimulating factor (hGM-CSF) was produced from cell suspension culture of transgenic tobacco which was transformed by using Agrobacterium harboring the hGM-CSF gene. To improve the production of hGM-CSF in batch culture system, the effects of initial sucrose concentration and inoculum size were investigated. The results show that the hGM-CSF production was not affected by small inoculum size in medium containing either low or high concentration of sucrose. However, the production of hGM-CSF was increased under increasing of the inoculum sizes and sucrose concentration. Under the combination of inoculum and sucrose concentration, the maximum hGM-CSF production of 720 $\mu$g/L was obtained at 90 g/L of initial sucrose concentration and 110 g/L of inoculum size.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.25 no.12
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• pp.1920-1926
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• 2021

The electronic inclinometer can measure and print various output data related to the ship's heel, rolling cycle, and amplitude. The electronic inclinometer that can support ship stability judgment is equipped with a platform that supports stability for recovery performance, so it is possible to provide data even for small ships that lack ship stability judgment information. GM is an important factor in determining stability, and each type of ship has different GM scope. The purpose of this paper is to analyze GM according to the type of target ship and to review for a stable GM proposal. In addition, it is expected that GM data for each ship type will be embedded in the electronic inclinometer for ship that meets international standards, and will be used as data for securing and reviewing GM for strengthening ship safety