• Title/Summary/Keyword: GLV

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Incidence of Viral Infection of Main Garlic Growing Areas of Gangwon Province in Korea (강원도 마늘 주산지 재배마늘의 바이러스 감염 실태)

  • Shin, Ji-Eun;Lee, Yeong-Gyu;Kim, Byung-Sup
    • Research in Plant Disease
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    • v.19 no.3
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    • pp.233-236
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    • 2013
  • This research was conducted to investigate the incidence of viral infection in Samcheok-shi and Jeongseon-gun, main garlic growing areas of Gangwon province in Korea. Four primers sets (GLV, LYSV, GCLV, OYDV) were used for RT-PCR to test of 100 samples collected from each location. Infection rates of GLV, LYSV, GCLV and OYDV of garlic samples from Samcheok-shi and Jeongseon-gun were 95, 95, 92 and 33%, respectively. All garlic samples tested in this research were infected at least one virus. Coinfection of 3 kinds of virus (GLV, LYSV and GCLV) and 4 kinds of virus (GLV, LYSV, GCLV and OYDV) were 60 and 25%, respectively. While infections of GLV, LYSV and GCLV were evenly found in both two regions, OYDV infection of garlic samples collected from Jeongseon-gun was higher than Samcheok-shi. Viral infections at garlic fields of whole country and Gangwon province are very serious now. Therefore, it is very necessary to develop technic for virus-free bulb and renew seed bulb of garlic.

Immunological Detection of Garlic Latent Virus (마늘 잠복 바이러스의 면역학적 진단)

  • Choi, Jin-Nam;Song, Jong-Tae;Song, Sang-Ik;Ahn, Ji-Hoon;Choi, Yang-Do;Lee, Jong-Seob
    • Applied Biological Chemistry
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    • v.38 no.1
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    • pp.49-54
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    • 1995
  • To understand the molecular structure and pathogenesis mechanism of Korean garlic viruses, we have isolated cDNA clones for garlic viruses. The partial nucleotide sequences of 24 cDNA clones were determined and those of five clones containing poly(A) tail were compared with sequences of other plant viruses. One of these clones, V9, has a primary structure similar to the carlavirus group, suggesting that the clone V9 derived from a part of garlic latent virus (GLV). Northern blot analysis with the clone V9 as a probe demonstrated that GLV genome is 8.5 knt long and has a poly(A) tail. The clone V9 encodes coat protein (CP) of 33 kDa and nucleic acid binding protein of 10 kDa in different reading frame. The hexanucleotide motif, 5'-ACCUAA, which is conserved in the 3' noncoding region arid was proposed to be a cis-acting element involved in the production of negative strand genomic RNA was noticed. Complementary sequence to the hexanucleotide motif, 5'-TTAGGT, is also found in the positive strand of V9 RNA. The putative CP gene was cloned into the pRSET-A expression vector and expressed in E. coli BL21. The expressed recombinant V9CP protein was purified by $Ni^{2+}$ NTA affinity chromatography. The anti-V9CP antibody recognizes 34 kDa polypeptide which could be CP of GLV in infected garlic leaf extract. Immunoblot and Northern blot analysis of various cultivars shows wide occurrence of GLV in Korean garlic plants.

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Purification and Amplification of Garlic Latent Virus (마늘 잠복 바이러스의 순수분리 및 증식)

  • 최진남
    • Journal of Plant Biology
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    • v.35 no.3
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    • pp.259-263
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    • 1992
  • 한국 마늘에 감염되어 있는 주된 바이러스 중의 하나로 알려진 마늘 잠복 바이러스 (GMV)의 분자 구조와 병 발생 메카니즘을 이해하기 위하여, 국부 감염 숙주 식물인 Vicia faba에 연속적으로 감염시킴으로써 마늘 잠복 바이러스를 정제하였고, GMV에 대한 전신 감염성 숙주 식물로 생각되는 leek에서 GLV를 대량으로 증식시켰다. 투과전자현미경을 이용하여 바이러스 입자를 관찰한 결과, 마늘 바이러스들의 입자의 길이는 200-2000 nm의 분포를 보였으나 입자의 대부분은 600-900 nm의 범위 안에 존재하였다. 반면 순수 분리된 GLV 입자는 평균 690 nm의 길이를 보여주었고, 유연한 실 모양이었다. SDS-PAGE 분석으로 혼합 감염된 마늘 잎으로부터 분리된 마늘 바이러스의 구조 단백질은 분자량 24,500-38,000 Da의 분포를 나타내었으나, GLV 껍질 단백질의 분자량은 34,000 Da으로 나타났다.

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Faster Ate Pairing Computation over Pairing-Friendly Ellipitic Curves Using GLV Decomposition

  • Eom, Soo Kyung;Lee, Eunjeong;Lee, Hyang-Sook
    • ETRI Journal
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    • v.35 no.5
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    • pp.880-888
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    • 2013
  • The preexisting pairings ate, $ate_i$, R-ate, and optimal-ate use q-expansion, where q is the size of the defining field for the elliptic curves. Elliptic curves with small embedding degrees only allow a few of these pairings. In such cases, efficiently computable endomorphisms can be used, as in [11] and [12]. They used the endomorphisms that have characteristic polynomials with very small coefficients, which led to some restrictions in finding various pairing-friendly curves. To construct more pairing-friendly curves, we consider ${\mu}$-expansion using the Gallant-Lambert-Vanstone (GLV) decomposition method, where ${\mu}$ is an arbitrary integer. We illustrate some pairing-friendly curves that provide more efficient pairing from the ${\mu}$-expansion than from the ate pairing. The proposed method can achieve timing results at least 20% faster than the ate pairing.

Speeding up Scalar Multiplication in Genus 2 Hyperelliptic Curves with Efficient Endomorphisms

  • Park, Tae-Jun;Lee, Mun-Kyu;Park, Kun-Soo;Chung, Kyo-Il
    • ETRI Journal
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    • v.27 no.5
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    • pp.617-627
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    • 2005
  • This paper proposes an efficient scalar multiplication algorithm for hyperelliptic curves, which is based on the idea that efficient endomorphisms can be used to speed up scalar multiplication. We first present a new Frobenius expansion method for special hyperelliptic curves that have Gallant-Lambert-Vanstone (GLV) endomorphisms. To compute kD for an integer k and a divisor D, we expand the integer k by the Frobenius endomorphism and the GLV endomorphism. We also present improved scalar multiplication algorithms that use the new expansion method. By our new expansion method, the number of divisor doublings in a scalar multiplication is reduced to a quarter, while the number of divisor additions is almost the same. Our experiments show that the overall throughputs of scalar multiplications are increased by 15.6 to 28.3 % over the previous algorithms when the algorithms are implemented over finite fields of odd characteristics.

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Physico-chemical properties of green leaf volatiles (GLV) for ascertaining atmospheric fate and transport in fog

  • Vempati, Harsha;Vaitilingom, Mickael;Zhang, Zenghui;Liyana-Arachchi, Thilanga P.;Stevens, Christopher S.;Hung, Francisco R.;Valsaraj, Kalliat T.
    • Advances in environmental research
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    • v.7 no.2
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    • pp.139-159
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    • 2018
  • Green Leaf Volatiles (GLVs) is a class of biogenically emitted oxygenated hydrocarbons that have been identified as a potential source of Secondary Organic Aerosols (SOA) via aqueous oxidation. The physico-chemical properties of GLVs are vital to understanding their fate and transport in the atmosphere via fog processing, but few experimental data are available. We studied the aqueous solubility, 1-octanol/water partition coefficient, and Henry's law constant ($K_H$) of five GLVs at $25^{\circ}C$: methyl jasmonate, methyl salicylate, 2-methyl-3-buten-2-ol, cis-3-hexen-1-ol, and cis-3-hexenyl acetate. Henry's law constant was also measured at temperatures and ionic strengths typical of fog. Experimental values are compared to scarcely-available literature values, as well as estimations using group and bond contribution methods, property-specific correlations and molecular dynamics simulations. From these values, the partition coefficients to the air-water interface were also calculated. The large Henry's law constant of methyl jasmonate ($8091{\pm}1121M{\cdot}atm^{-1}$) made it the most significant GLV for aqueous phase photochemistry. The HENRYWIN program's bond contribution method from the Estimation Programs Interface Suite (EPI Suite) produced the best estimate of the Henry's constant for GLVs. Estimations of 1-octanol/water partition coefficient and solubility are best when correlating an experimental value of one to find the other. Finally, the scavenging efficiency was calculated for each GLV indicating aqueous phase processing will be most important for methyl jasmonate.

FROBENIUS ENDOMORPHISMS OF BINARY HESSIAN CURVES

  • Gyoyong Sohn
    • East Asian mathematical journal
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    • v.39 no.5
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    • pp.529-536
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    • 2023
  • This paper introduces the Frobenius endomophisms on the binary Hessian curves. It provides an efficient and computable homomorphism for computing point multiplication on binary Hessian curves. As an application, it is possible to construct the GLV method combined with the Frobenius endomorphism to accelerate scalar multiplication over the curve.

Flat Panel Display를 위한 구동방식 및 구동회로

  • 권오경
    • The Magazine of the IEIE
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    • v.22 no.3
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    • pp.92-105
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    • 1995
  • 각 분야에서 고화질 고해상도의 Flat Panel Display(FPD)에 대한 요구가 급증하고 있으며, FPD 소자들도 다양하다. FPD를 위한 구동방식은 근본적으로 matrix addroessing 방식을 사용하지만 FPD 소자의 동작 원리, 특성, 구동상의 문제점 등에 따라서 구동방식도 매우 다양하다. 다양한 FPD 소자들이 구동 방식과 구동회로를 다 살펴볼 수 없으므로 본고에서는 FPD소자인 PDP, EL, LED, EC, LCD, DMD, AMA, GLV, FED 등의 동작 원리를 설명하고, 이 소자들 중에서 AMLCD와 dc-PDP 소자의 구동방식과 구동회로에 대하여 논의한다. AMLCD의 구동방식으로는 펄스 구동 방식과 용량 구동 방식을 살펴보고, AMLCD의 문제점들을 극복할 수 있는 방법들을 설명한다. dc-PDP의 구동 방법인 pulse-memory 구동방식과 새로운 pulse-memory 구동 방식을 살펴보았다.

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MEMS 기술을 이용한 프로젝션 TV의 개발동향과 전망

  • 최범규
    • 전기의세계
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    • v.45 no.9
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    • pp.37-41
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    • 1996
  • MEMS 기술에 의해 개발되고 있는 많은 제품들 가운데 가장 잠재력 있고 큰 시장을 가진 대형 projector의 개발은 핵심 chip이 현재 DMD(Digital Micromirror Device), AMA(Actuated Mirror Array), 그리고 GLV(Grating Light Valve) 방식으로 진행되고 있으며 TI사가 개발 중인 DMD projector가 상품화에 근접해 있으며 고화질의 시제품을 SID(Society for Information Display) 국제 전시회에 출품하여 전세계 연구원들의 이목을 끌었으며 앞으로 어떻게 수율을 높이고 광학계의 단순화를 이루어 제작비를 낮추는 가가 관심의 초점이다. MEMS 연구를 하는 한 사람으로서의 사견으로도 이 제품이 성공하여야 범세계적으로 일어나고 있는 MEMS 기술이 더욱 확실한 신기술로서의 지위를 갖고 연구되리라 믿는다.

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Molecular cloning of cDNAs for Korean garlic viruses

  • Choi, Jin-Nam;Ahn, Ji-Hoon;Choi, Yang-Do;Lee, Jong-Seob
    • Applied Biological Chemistry
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    • v.36 no.4
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    • pp.315-317
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    • 1993
  • To understand the molecular structure and pathogenesis mechanism of Korean garlic viruses (GV), virus particles were isolated from field-grown garlic leaves and RNA genome was isolated from them. It was used for constructing cDNA library for GV. Several cDNA clones for GV were isolated and classified into 4 different groups on the basis of cross Southern hybridization. Northern blot analysis of GV RNA with one of these cDNA clones shows that the clone is a cDNA for GV RNA.

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