• Journal of Ginseng Research
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• 제29권2호
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• pp.107-112
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• 2005

본 실험은 수분함량 $15\%$로 건조한 수삼을 사출구온도($100^{\circ}C$와 $120^{\circ}C$), 스크루 회전속도(200과 300 rpm)에서 압출성형처리하여 조사포닌,진세노사이드, 말톨, 환원당, 색도와 같은 홍삼성분의 변화를 분석하였다. 압출성형공정을 통해 조사포닌 함량이 증가하는 것으로 보아사포닌의 압출성형공정동안 생성되는 것을 알 수가 있었다. 홍삼에 존재하는 진세노사이드 $Rg_l$과 $Rg_2$는 사출구온도를 $100^{\circ}C$에서 $120^{\circ}C$로 증가함에 따라 0.227 mg/g에서 0.2835 mg/g$(Rg_l)$, 0.1164 mg/g에서 0.2230 mg/g$(Rg_2)$증가하였고, 스크루 회전속도를 300rpm에서 200rpm으로 감소시켰을 때 진세노사이드 $Rg_1$ 과 $Rg_2$는 증가하는 경향을 나타내었다. 또한 수삼이나 백삼에는 함유되지 않고 홍삼에만 함유된 특이성분으로 알려진 말톨을 압출성형 수삼에서 확인할 수가 있었다. 사출구의 온도가 $100^{\circ}C$에서 $120^{\circ}C$로 증가함에 따라 총당의 함량은 크게 변화가 없었지만 환원당은 온도가 증가할수록 감소하는 경향을 보였다. 결론적으로 사출구온도와 스크루 회전속도 이외의 압출성형 공정변수의 조절을 통해 압출성형공정을 홍삼제조 공정에 적용할 수 있는 가능성을 알 수가 있었다.

• 대한의생명과학회지
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• 제21권2호
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• pp.103-114
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• 2015

In this study, we investigated the effect of rice bran water extract fermented with Lactobacillus plantarum KCCM-12116 (RBLp) on ADP ($20{\mu}M$)-, collagen ($10{\mu}g/mL$)-, and thrombin (0.2 U/mL)-stimulated platelet aggregation. RBLp dose-dependently inhibited ADP-, collagen-, and thrombin-induced platelet aggregation, with $IC_{50}$ values of 501.1, 637.2, and > $2,000{\mu}g/mL$, respectively. The platelet aggregation induced by ADP plus RBLp ($750{\mu}g/mL$) was increased by the adenylate cyclase inhibitor, SQ22536, and the cAMP-dependent protein kinase (A-kinase) inhibitor, Rp-8-Br-cAMPS. Treatment with RBLp increased the phosphorylation of VASP ($Ser^{157}$), an A-kinase substrate, which was also inhibited by SQ22536 and Rp-8-Br-cAMPS. It is thought that the RBLp-induced increases in cAMP contributed to the phosphorylation of VASP ($Ser^{157}$), which in turn resulted in an inhibition of ADP-induced platelet aggregation, thereby indicating that RBLp has an antiplatelet effect via cAMP-dependent phosphorylation of VASP ($Ser^{157}$). Thus, RBLp may have therapeutic potential for the treatment (or prevention) of platelet aggregation-mediated diseases, such as thrombosis, myocardial infarction, atherosclerosis, and ischemic cerebrovascular disease.

• Mycobiology
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• 제30권2호
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• pp.61-64
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• 2002

A Monokaryotic strain G8M without clamp connections was isolated from germinated basidiospore that was obtained from cultivated fruit body. Strain G8M was used as a tester isolate for 'dikaryon-monokaryon mating'(di-mon mating) with the strains of Ganoderma lucidum, G6 and G35(Korean wild strains), G3(Taiwan), G4(Canada), G15(America), G. oregonense G24, G. resinaceum G28, G. oerstedii G23, and G. subamboinense G29. Isolate G8M was compatible to Korean strains G6 and G35, but was incompatible to foreign strains G3, G4, or G15. Compatible reactions between strains were readily observed macroscopically. Clear barrage lines formed between incompatible strains. These clear lines were not apparent in compatible di-mon matings. The Korean strains were morphologically distinct; they did not form any chlamydospores, and stopped growth at $35^{\circ}C$. The strains of G. lucidum from Korea may be considered as different species from Taiwan, Canadian and American cultures.

• 한국수정란이식학회지
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• 제23권3호
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• pp.197-201
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• 2008

In order to provide information of genetic variants for Haptoglobin (Hp) gene, which may be related to weight traits in pig, a total of 235 animals from National Institute of Animal Science (NIAS) were screened with 3 primers. The primer sequences were selected using the porcine cDNA sequences based on NM_214000, and the exon boundaries were estimated. Genetic variants were observed using direct sequencing analysis, and there were 9 SNPs detected at nucleotide positions 503 (A/G), 509 (A/G), 709 (C/T), 734 (C/A), 742 (G/A), 769 (A/G), 840 (C/T), 876 (C/T) and 882 (C/A). All the SNPs were located in coding regions, and mutations caused amino acid changes at nucleotide positions 503, 509, 734, 742 and 769. Allele frequencies of SNPs were estimated for all segments. The SNPs at nucleotide position 509 (p<0.0001) and 734 (p<0.05) were significantly associated with average daily gain, but no significance was observed with other SNPs. From the results, the identified SNPs may be a useful candidate marker for the porcine weight gain traits.

• Journal of Microbiology and Biotechnology
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• 제17권7호
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• pp.1106-1112
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• 2007

This report provides the complete nucleotide sequences of the full-length cDNA encoding squalene synthase (SQS) and its genomic DNA sequence from a triterpene-producing fungus, Ganoderma lucidum. The cDNA of the squalene synthase (SQS) (GenBank Accession Number: DQ494674) was found to contain an open reading frame (ORF) of 1,404 bp encoding a 468-amino-acid polypeptide, whereas the SQS genomic DNA sequence (GenBank Accession Number: DQ494675) consisted of 1,984 bp and contained four exons and three introns. Only one gene copy was present in the G. lucidum genome. The deduced amino acid sequence of Ganoderma lucidum squalene synthase (GI-SQS) exhibited a high homology with other fungal squalene synthase genes and contained six conserved domains. A phylogenetic analysis revealed that G. lucidum SQS belonged to the fungi SQS group, and was more closely related to the SQS of U. maydis than to those of other fungi. A gene expression analysis showed that the expression level was relatively low in mycelia incubated for 12 days, increased after 14 to 20 days of incubation, and reached a relatively high level in the mushroom primordia. Functional complementation of GI-SQS in a SQS-deficient strain of Saccharomyces cerevisiae confirmed that the cloned cDNA encoded a squalene synthase.

• 한국식품영양과학회지
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• 제38권7호
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• pp.946-950
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• 2009

본 연구는 압출성형 공정을 통한 인삼 시료의 성분 변화를 측정하기 위하여 일반성분 및 항산화활성을 측정하였다. 수분함량을 20%로 고정시키고, 배럴온도는 100, $140^{\ciirc}C$로 변화시켜 압출성형백삼을 제조하였다. 백삼, 홍삼, 압출성형백삼의 일반성분 실험에서는 압출성형 공정을 통한 성분의 증가를 확인할 수 없었다. 항산화활성 측정 결과 산성다당체 함량은 홍삼이 62.94 mg/mL로 높게 측정되었고 총 폴리페놀함량과 DPPH 측정에서는 $140^{\circ}C$ 압출성형백삼이 각각 14.99 mg/g, 80.21%로 높게 측정되어 압출성형 공정을 통한 항산화 물질의 증가를 확인할 수 있었다. $140^{\circ}C$ 압출성형백삼의 조성 분석을 위하여 BuOH 분획을 실시한 결과 총페놀성 화합물과 산성다당체 측정 결과가 각각 27.2 mg/g, 217.6 mg/mL로 측정되었으며 HPLC를 통해 알아본 사포닌 조성은 $140^{\circ}C$ 압출성형백삼에서 모든 성분의 증가를 확인할 수 있었고 특히 홍삼의 특유성분인 Rg3s, Rg3r의 증가로 압출성형 공정을 통한 백삼의 홍삼화 가능성을 확인할 수 있었다.

• 한국식생활문화학회지
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• 제20권1호
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• pp.123-129
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• 2005

The purpose of this study was to investigate the effect of dietary Morus alba L.(Bong-ip, B), Glycyrrhizae glabra(Gam-chei, C), Pinus densiflora(Sol-lp, S) and Angelica gigas(Dang-gi, D)powder on serum composition in rats(Sprague-Dawley male rats, 100-110g). Serum TG(triglyceride, p<0.01), total cholesterol, glucose, total protein, albumin, GGT$({\gamma}-glutamyl$ transferase, p<0.05) were significantly increased D group than that of nomal and other groups, but UA(uric acid, p<0.05) was significantly decreased, and C group(p<0.05) was significantly increased. but C group of urine(p<0.05) was significantly decreased. Also, B and S groups(p<0.05) of BUN(blood urea nitrogen), S group(p<0.05) of ALP(alkaline phosphokinase, Band C(p<0.05) of CPK(creatinine phosphokinsae, p<0.05) were significantly increased. B, S and C groups were better than D group for lipid metabolism, and pretection to liver. Also, B and C groups of glucose were same as normal diet, so Morus alba L. was good food for lipid metabolism and hypoglycemic effect.

• 대한전기학회논문지:전기물성ㆍ응용부문C
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• 제51권8호
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• pp.335-339
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• 2002

Ba(Mgl-xCox)TaO3[BMCT] ceramics were prepared by the conventional mixed oxide method. The ceramics were sintered at the temperature of 1525~$1625^{\circ}C$ for 5hr. in air. The crystal structure of BMCT ceramics was investigated by the XRD. The microstructure of the specimens were observed by SEM. The Microwave dielectric properties of BMCT specimens were investigated as a function of composition and sintering temperature. All BMCT ceramics sintered over 1575$^{\circ}C$ were showed a polycrystalline complex perovskite structure. The density of BMCT (90/10) specimen sintered at $1575^{\circ}C$ was 7.75g/㎤. As the Co contents decreased, the ordering parameter of B-site in BMCT increased. In the case of the BMCT(90/10) ceramics sintered at $1575^{\circ}C$ for 5 hours, dielectric constant, quality factor and temperature coefficient of resonant frequency for microwave dielectrics application were a good value o( 25, 17, 845 at 10㎓ and +2.4 ppm/${\circ}$, respectively.

• Journal of Microbiology and Biotechnology
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• 제23권2호
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• pp.156-160
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• 2013

Culture supernatants of splenocytes from C57BL/6 mice were exposed to 0.3, 1.0, and 3.0 ${\mu}g/ml$ cordycepin plus 3.0 ${\mu}g/ml$ lipopolysaccharide (LPS) to investigate the effects of cordycepin (3'-deoxyadenosine) on the production of inflammatory cytokines. Coadministration of 3.0 ${\mu}g/ml$ cordycepin with LPS in cultured murine spleen cells significantly diminished expression of the inflammatory cytokines tumor necrosis factor-${\alpha}$ and interleukin-6 (IL-6) in a time-dependent manner. Expression of the inflammatory cytokine IL-17A was substantially downregulated in a timeand concentration-dependent manner at all cordycepin concentrations. These findings suggest that cordycepin downregulates the immediate hypersensitivity reaction stimulated by LPS.

• 한국식품과학회지
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• 제21권6호
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• pp.808-814
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• 1989

Mortierella isabellina IFO 8183을 이용하여 ${\gamma}-linolenic$ acid를 생산하기 위한 최적 배양조건을 검토한 결과, 최적 탄소원과 최적 질소원은 각각 glucose와 $NH_4NO_3$이었으며, 지질 생산배지내의 최적 C/N율은 40이었다. 또한, 최적 초기 pH와 배양온도는 각각 6.0과 $30^{\circ}C$이었으며, 균체내에 축적된 지질의 지방산 조성은 pH와 온도에 의해 변화되었다. Sodium acetate를 배양 초기에 0.5%(w/v) 첨가하였을 때 대조구에 비해 ${\gamma}-linolenic$ acid 함량과 ${\gamma}-linolenic$ acid 생성량이 각각 15.4%와 37.8% 증가되었다. 최적 조건하에서 8일간 진탕배양하였을 때 균체수율, 지질수율, 지질함량, ${\gamma}-linolenic$ 함량 및 ${\gamma}-linolenic$생성량은 각각 0.347g/g, 0.18g/g, 0.52g/g, 60mg/g 및 347mg/l이었다.