• Korean Journal of Plant Resources
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• v.17 no.3
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• pp.352-357
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• 2004

This study was carried out to investigate the effects of temperature and photoperiod on the growth of Fagopyrum tataricum. It showed a tendency to promote the germination rate and plant height of tartary buckwheat under high temperature. Plant height and number of leaves were promoted in the order of 25 ＆gt; 20 ＆gt; 15 ＆gt; 3$0^{\circ}C$ under the different temperature after 30 days of the planting. In the 10, 12, 14hr photoperiods at 2$0^{\circ}C$, plant height, number of leaves and dry weight were increased as much as photoperiod became long except by 16hr. Rutin contents was not regular both common and tartary buckwheat under the photoperiods. The rutin content in leaves was higher than stem. Compared to yield of tartary and common buckwheat, plant height, number of leaves, number of branch and stem diameter of tartary buckwheat increased more than common buckwheat. Especially, number of leaves was increased about 2.5 times more than common buckwheat. And there was a difference in about 2.1 times in grain weight and about 5.4 times in number of grains. Rutin content was 1469.8mg/l00g in grain of tartary buckwheat. It was about 60 times higher than 22mg/100g in grain of common buckwheat.

• Korean Journal of Plant Resources
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• v.31 no.4
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• pp.372-377
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• 2018

This study was investigated the effect of immature rhizome production according to harvest times-based treatment method for seed production of Gastrodia elata. The results revealed that when the tuber weight of G. elata harvested in spring (GEHS) was ${\geq}100g$, the rate of artificial fertilization, protocorm formation, and immature rhizome formation was 90.9%-94.8%, 3.1%-5.4%, and 10.1%-15.3%, respectively. When G. elata harvested in fall (GEHF) was treated at a low temperature for 4 weeks or more, the rate of artificial fertilization, protocorm formation, and immature rhizome formation was 70.4%-87.6%, 2.2%-2.6%, 8.7%-9.5%, respectively. Therefore, to produce seeds and immature rhizomes, GEHS must have tubers of more than 100 g, whereas GEHF requires breaking dormancy by low-temperature treatment for 4 weeks or more. Compared with those of GEHS, the rate of artificial fertilization, protocorm formation, and immature rhizome formation was lower in GEHF; however, it was higher than those in the natural germination state. Thus, it can be expected that G. elata can be produced throughout the year by ensuring that the seeds and immature rhizomes of G. elata are produced using a constant tuber weight and by breaking dormancy with low temperature treatment.

• Journal of Korean Society of Forest Science
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• v.110 no.4
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• pp.577-589
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• 2021

Soil seed bank community contributes to the long-term conservation of plant diversity and vegetation dynamics, and their decreasing diversity and density with soil depth provide critical perspectives (deterministic and stochastic) for understanding the community disassembly process. We analyzed changes in species composition and diversity and structuring patterns by soil layer (top and bottom), including surface vegetation, in Gwangneung Forest, a mature forest with a vegetation climate in the temperate central part of the Korean Peninsula. From two layers of soil collected with a vertical difference of 10 cm, 934 specimens of 27 families, 40 genera, 44 species, three varieties, and 47 taxa, germinated. Although species diversity and germination density decreased in most comparative characteristics, including growth type, there was no statistical significance due to large deviations. Within-group variability of species composition was similar in the upper and lower soils, as was the decline pattern in co-occurred species (ζ-diversity) and change in species retention probability. The structuring process of the community composition in the two soil layers was fitted with an exponential correlation rather than a power function, demonstrating the dominance of the stochastic process. The pattern in diversity and species turnover according to soil depth in Gwangneung Forest was discovered to be structured by stochastic random events, such as seed vertical movement rather than interaction with trait characteristics.

• Molecules and Cells
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• v.44 no.10
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• pp.770-779
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• 2021

Transgenic Arabidopsis thaliana expressing an anti-rabies monoclonal antibody (mAb), SO57, was obtained using Agrobacterium-mediated floral dip transformation. The endoplasmic reticulum (ER) retention signal Lys-Asp-Glu-Leu (KDEL) was tagged to the C-terminus of the anti-rabies mAb heavy chain to localize the mAb to the ER and enhance its accumulation. When the inaccurately folded proteins accumulated in the ER exceed its storage capacity, it results in stress that can affect plant development and growth. We generated T₁ transformants and obtained homozygous T₃ seeds from transgenic Arabidopsis to investigate the effect of KDEL on plant growth. The germination rate did not significantly differ between plants expressing mAb SO57 without KDEL (SO plant) and mAb SO57 with KDEL (SOK plant). The primary roots of SOK agar media grown plants were slightly shorter than those of SO plants. Transcriptomic analysis showed that expression of all 11 ER stress-related genes were not significantly changed in SOK plants relative to SO plants. SOK plants showed approximately three-fold higher mAb expression levels than those of SO plants. Consequently, the purified mAb amount per unit of SOK plant biomass was approximately three times higher than that of SO plants. A neutralization assay revealed that both plants exhibited efficient rapid fluorescent focus inhibition test values against the rabies virus relative to commercially available human rabies immunoglobulins. KDEL did not upregulate ER stress-related genes; therefore, the enhanced production of the mAb did not affect plant growth. Thus, KDEL fusion is recommended for enhancing mAb production in plant systems.

• Journal of Korean Society of Forest Science
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• v.110 no.3
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• pp.295-307
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• 2021

This study proposed a field-applicable natural regeneration method using natural seeding to afforest a secondary growth forest after felling in Larix kaempferi plantations. In 2016, an experimental site was established usingseed-tree and clear-cutting methods in Gapyeong, Gyeonggi-do, South Korea. Characteristics of seed fructification and inflow in the natural regeneration site were analyzed along with an experimental site in Bonghwa, Gyeongsangbuk-do, which was established in 2014. As a result, the number of seeds per individual tree of V age class L. kaempferi was 690,340 seeds in a good year, with an estimated 13.8 million seeds per ha fruited in natural regeneration sites of L. kaempferi with 25 seed-trees per ha. Seed dispersal began in September and germination rates of seeds dispersed until November were greater than 40%. Reserved seed-tree sites showed the highest amount of seed inflow, followed by the seed-tree site and clear-cut site in descending order. The reserved seed-tree method performed better than other methods for evenly scattering seeds. Therefore, the timing of logging and seedbed generation for natural regeneration of L. kaempferi is estimated to be most effective before September when seeds are purposefully scattered using the reserved seed-tree method as an effective method for uniform seed supply.

• Korean Journal of Plant Resources
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• v.34 no.4
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• pp.278-286
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• 2021

Efficient in vitro regeneration system is essential for the successful crop breeding of soybean (Glycine max (L.) Merr.) using the new biotechnology. The genotype of donor plants strongly influences the establishment of tissue culture system. Therefore, the screening of genotypes with excellent tissue culture ability is very important for soybean genetic improvement. In this study, we report the tissue culture efficiency of 21 soybean cultivars belong to Korean soybean core-collection and two foreign cultivars (Jack and Maverick). The Kwangan, Anpyeong and Seonam are share close genetic relationship in 21 cultivars and these three cultivars were observed the high frequency of germination and regeneration. Furthermore, the high tissue culture abilities were also observed in the Williams 82 used in reference genome sequencing and the two foreign cultivars. The transformation of pBAtc:tRNA with bar gene was performed by Agrobacterium tumefaciens in the cultivars with high tissue culture ability. Transformation of the bar gene was identified by PCR analysis in Kwangan, Pungwon, Seonam, and Maverick. Our results provide useful information for the breeding of various soybean cultivars by plant biotechnology such as, genome editing.

• Korean Journal of Breeding Science
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• v.43 no.6
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• pp.559-563
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• 2011

Cymbidium 'Purple Star' (Miake 'Pieta' ${\times}$ 'Allstar Mariane') was developed from a cross between hybrids at the National Institute of Horticultural & Herbal Science (NIHHS), Rural Development Administration (RDA) in 2008. A cross was made between purple colored flower C. Miake 'Pieta' as maternal line and dark pink colored flower, C. 'Allstar Mariane' as paternal line in 1995. The seed germination, cultivation, selection, and characteristic trials were conducted from 1996 to 2002. The line was named as Wongyo 'F1-21' and phenotype was characterized in 2007 as a new cultivar. The 'Purple Star' has dark purple basal color (RHS, GP186A) on both of sepal and petal with red lip (RHS, RP59A). 'Purple Star' has about 10.7 flowers per flower stalk and flower size of 7.6 cm. General appearance of petals and sepals is slightly incurved shape. The plant size is intermediate having erect peduncle. Blooming is started from the late of January (mid-winter) under optimal culture condition. Leaf attitude and twisting is erect and weak respectively. This hybrid has attractive floral arrangement, long flower stalk (78.4 cm) and vigorous growth. 'Purple Star' has a great potential for exporting to Chinese market.

• Korean Journal of Breeding Science
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• v.42 no.5
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• pp.463-469
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• 2010

Rice is a staple food for more than 50% of the world's population. Embryo comprises only 2 to 3% of the weight distribution of the entire pericarp but has higher concentration of vitamins, proteins, and essential fatty acids than the other parts of grains. Moreover, ${\alpha}$-tocoperol, ${\gamma}$-oryzanol, phytic acid and ${\gamma}$-aminobutric acid that have nutraceutical value are abundant. Increasing the volume of embryo assures the fortification of nutritional value of rice grain. We developed new black waxy giant embryo rice, Milyang 263 by crossing Josaengheugchal, a black waxy rice variety, and $ge^t$, a giant embryo mutant generated by tissue culture. The nutrient contents and physical properties of Milyang 263 were compared with several giant embryo mutants and normal embryo rice varieties. Changes in the nutrient properties after germination were also observed. Results indicated that this new black waxy giant embryo rice, Milyang 263, offers a promising source for improving nutritional quality of rice especially anthocyanin, essential minerals, and GABA.

• Korean Journal of Environment and Ecology
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• v.33 no.1
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• pp.52-63
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• 2019

This study analyzed the characteristics of vegetation structure of the camphor tree (Cinnamomum japonicum) community in the area of mount Tachibana, Kasuya county, Fukuoka Prefecture designated as a special natural monument in Japan. The survey showed overwhelming dominance of canopy tree in the canopy layer (about 30 m in tree heights and 92.79 cm in average breast height diameter) but no appearance in the understory layer or the shrub layer. In the understory layer and the shrub layer, Castanopsis sieboldii, Machilus thunbergii, Neolitsea sericea, and Cinnamomum yabunikkei, which were the competing species to the canopy layer and the late-successional species in the warm temperate climate zone, were mainly distributed. Moreover, the species diversity was generally low, indicating the vegetation characteristics that was not typical of evergreen broad-leaved forests. This is presumably because camphor trees were actively planted, protected, and cultivated to produce camphor which was valuable in the past. Although this site has not been artificially managed for the past 90 years as the raw materials of camphor have not been collected, vegetation transition did not proceed, which is unique. It is probably due to the fact that camphor was overwhelmingly dominant in the canopy layer so that the inflows of species were restricted, and young tree germination did not occur due to the allelopathy effects of camphor trees.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.43-43
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• 2019

Deparia pycnosora (Christ) M. Kato is a fern used as ornamental plant. In addition, it is called "Teol-go-sa-ri" in Korean name. The aim of this study was to develop a practical propagation method of D. pycnosora using tissue culture technique. Prothallus obtained from spore germination was the used as experiment materials. The prothalli (300 mg) used in all experiments were sub-cultured for 8-week intervals. The most suitable media for prothallus propagation were identified by culturing 300 mg of prothalli in $1/4{\times}$, $1/2{\times}$, $1{\times}$, $2{\times}$ MS medium and in Knop medium for 8 weeks. Also, the prothalli were cultured by chopping with a scalpel. In addition, sucrose, activated charcoal, and total nitrogen source were added in different concentrations based on the culture medium selected. Cultures were maintained at a temperature of $25{\pm}1^{\circ}C$, light intensity of $30{\times}1.0{\mu}mol-m-2{\cdot}s-1$, and a photoperiod of 16/8 h (light/dark) in in vitro. The results showed that optimum was achieved prothallus fresh weight and development in $1{\times}$ MS medium. When other components were added to the basic $1{\times}$ MS medium, prothallus propagation was maximized in $1{\times}$ MS medium supplemented with 2% sucrose, 0.2% activated charcoal, and 60 mM total nitrogen. To select a suitable soil mixture for sporophyte formation, 1.0 g of prothallus was blended with distilled water, spread on five combinations of different soil substrates (decomposed granite, horticultural substrates, peat moss, and perlite), and cultivated for 12 weeks. The sporophyte cultures were maintained at a temperature of $25{\pm}1^{\circ}C$, light intensity of $43{\pm}2.0{\mu}mol-m-2{\cdot}s-1$, humidity of $84{\pm}1.4%$, and a photoperiod of 16/8 h (light/dark). As a results, horticultural substrate alone, 2:1 (v:v) mixtures of horticultural substrate and perlite, and 2:1 mixtures of horticultural substrate and decomposed granite induced 208.0, 201.3 and 248.8 sporophytes per pot, respectively. Therefore, this result could provide a practical mass propagation method of D. pycnosora