• Clinical and Experimental Reproductive Medicine
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• v.20 no.1
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• pp.45-51
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• 1993

Micromanipulation procedures have been used to improve fertilization rates in patients with male factor or with unexplained infertility. Partial zona disseetion(PZD), a method using mechanical force to open the zona pellucida increase the chances of fertilization. The purpose of this study is to increase rates of fertilization and pregnacy in the ART program by using PZD. The influence of PZD on the fertilization rate was investigated in 57 couples with semen defects, antisperm antibodies(ASA), or unknown factors. PZD directly performed in 35 couples with a history of fertilization failure in previous cycle (Group 1), and PZD applied in 22 couples with the failure of initial fertilization in the same cycle (Group 2). The fertilization rates of the male facor, ASA positive factor and unknown factor in Group 1 were 37.6%, 20.0% and 59.2%, respectively. The rates of fertilization of male factor, ASA positive factor and unknown factor in Group 2 were 34.8%, 20.0% and 26.5%, respectively. The incidences of polyspermy in Group 1 and Group 2 were 5.9% and 9.0%, respectively. Among 35 patients of Group 1, one patient was pregnant and successfully delivered, whereas 1 of 22 patients of Group 2 became pregnant, but aborted at 7 weeks.

• The Journal of Korean Obstetrics and Gynecology
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• v.20 no.4
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• pp.191-201
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• 2007

Purpose: The purpose of this study is to report the effect of Takrisodok-eum gami(托裏消毒飮加味: TRSDEGM) on Breast Abscess. Methods: One patient was a 35-year-old woman who complains pain, induration, burning sensation and skin flare of left breast, the other patient was a 49-year-old woman who complains pain, burning sensation and edema of right breast. Patients were treated by Herb medicine(TRSDEGM Water Extract). And the progress of symptoms was evaluated by checking the change of VAS, and inspecting the DITI. Results: After taking TRSDEKM, pain, induration, burning sensation, skin flare and edema were alleviated or resolved. And they haven#t recurred. Conclusion: TRSDEKM is expected to have positive effect on Breast Abscess.

• The Journal of Korean Obstetrics and Gynecology
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• v.21 no.3
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• pp.231-244
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• 2008

Purpose: The purpose of this study is to analyze body composition, obesity according to working pattern of working women. The subjects were workers in the automobile manufacturing industry. Methods: The subjects consisted of 514 industrial women workers who had received Korean oriental medicine health examination and submitted questionires about working pattern, during 8 months from April 2005 to November 2005. The body composition was measured by X-scan(Jawon medical, Seoul, Korea). We analyzed the relationship between body composition analysis, obesity and shift pattern with $X^2-test$ using the SPSS Ver. 12.0 Results: Regarding the realationship between body composition and working pattern, shift worker group had a significantly higher level of body weight, ratio of body fat, visceral fat and abdominal fat than normal worker. Conclusion: From this study, we can know that shift work can influence on working women's body composition and obesity.

• The Journal of Korean Obstetrics and Gynecology
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• v.26 no.3
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• pp.73-84
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• 2013

Objectives: This study aims to identify associated factors in female infertility treatment using herbal medicine, acupuncture and moxibustion. Methods: Acupuncture and moxibustion treatments were performed with intake of herbal medicines (Jokyungjongoktanggagam-bang, Sutaehwangagam-bang) for six menstrual cycles in 32 subfertile women. The variables selected for initial analysis were age, duration and type of infertility, frequency of previous In Vitro Fertilization (IVF) and live births, weekly frequency of sexual intercourse, number of treatment cycles, endometrial thickness on MCD15 and hormonal parameter (anti-mullerian hormone, follicle-stimulating hormone, luteinizing hormone, estrone). Results: Six patients became pregnant (18.75%) and seven patients withdrew. Factors influenced pregnancy were age (${\leq}35$ years), a shorter duration of infertility (<6 years), frequency of IVF (${\leq}3$), anti-mullerian hormone level (>1 ng/ml), and number of treatment cycles(${\leq}3$ cycles). Conclusions: Korean medicine is a useful and optimized treatment option for women with younger age, fewer previous IVF cycles, shorter infertile duration and normal anti-mullerian hormone level at first 3 cycles of treatment.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.2
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• pp.92-106
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• 2006

Purpose : To address the ability of Olibanum to induce cell death, we investigated the effect of olibanum on cell apoptosis. Twenty-four hours later, apoptosis occurred following olibanum exposure in a dose-dependent manner. Methods : We culture HeLa cell which is human metrocarcinoma cell in D-MEM included 10% fetal bovine serum(Hyclone Laboratories) below $37^{\circ}C$, 5% CO2. Then we observed apoptosis of log phage cell which is changed cultivation liquid 24 Hours periodically. Results : The treatment of BAPTA-AM regulated olibanum-induced apoptosis in HeLa human cervical carcinoma cells. The 24 hr-earlier -thapsigargin-pretreated cell showed the resistance against olibanum-induced apoptosis and the Ru360-mitochondrial uniporter-inhibited olibanum-induced apoptosis, too. It means that olibanum leads to the accumulation of calcium and the resultant apoptosis in HeLa cells. Immunoblotting data also shows that the expression of GRP78, ER stress marker protein, was induced by the olibanum. Bcl-2, anti-apototic protein, was decreased and that the expression of Bax, pro-apoptotic protein, was increased by the addition of olibanum. Interestingly, the olibanum increased the activity of caspase-8 as well as calpain cysteine pretense in HeLa cervical carcinoma cells. Calpain inhibitor-calpastatin as well as caspase-8C/A expression abrogated olibanum-induced apoptosis in the carcinoma cells. The inhibition of caspase-8 regulated olibanum-induced calpain activation but the inhibition of calpain did not have any effect on the caspase-8 activation in HeLa human cervical carcinoma cells. Conclusion : We conclude that olibanum induces the accumulation of calcium and the resultant apoptosis in which caspase-8 and calpain are involved.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.2
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• pp.77-91
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• 2006

Purpose : To address the ability of Dohaekseungkitang (DST: a commonly used herb formulation in Korea, Japan and China to have anti-cancer effect on cervical carcinoma), we investigated the effects of DST on programmed cell death (apoptosis) in HeLa human cervical carcinoma cells. Methods : We cultured HeLa cell which is human metrocarcinoma cell in D-MEM included 10% fetal bovine serum(Hyclone Laboratories) below $37^{\circ}C$, 5% CO2. Then we observed apoptosis of log phage cell which is changed cultivation liquid 24 Hours periodically. Results : After the treatment of DST for 48 hours, apoptosis occurred in a dose-dependent manner. In this study, we have shown that DST induces calpain and the associated caspase-8 and -9 activations. Apoptosis was prevented by pre-incubation of the cells with the calcium cHeLator-BAPTA-AM, calcium channel blocker-Nif edipine or Ryonidine agonist-Ryonidine peptide, implicating calcium in the apoptotic process. Ubiquitous calpains (mu- and m-calpain) have been repeatedly implicated in apoptosis, especially in calcium-related apoptosis. However this study showed 1hat either calpain inhibitor-calpastin or caspase-3 inhibitor-DEVD- did not blocked the herb formulation-induced apoptosis in HeLa human cervical carcinoma cells. D ST initiates a cell death pathway that is partially dependent of caspases. DST-induced apoptosis requires caspase-independent mechanism. Conclusion : We conclude that DST-induced calpain activation triggers the intrinsic apoptotic pathway in which caspase-independent mechanism is also involved.

• The Journal of Korean Obstetrics and Gynecology
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• v.22 no.3
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• pp.236-245
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• 2009

Purpose: The purpose of this study was to report the effect of TKM (Traditional Korean Medicine) to abdominal pain after laparoscopic gynecological surgery(LGS). Methods: 10 patients who visited the department of gynecology in OO medical center from 1st August 2007 to 31st December 2008 with abdominal pains after laparoscopic operation. They complained abdominal pain and other pains such as back pain, shoulder pain and vaginal bleeding etc. We treated patients with herb medicine, acupuncture and moxibustion treatment. The progress of signs and symptoms was evaluated by checking the change of visual analog scale(VAS). Results: The mean age was 45.1 years(range 38-49), parity 2(0-3) and previous abdominal surgery case was 5. The mean of hospital admitting day was 20 days(range 9- 51) and taken for reducing VAS 10 to 3 were 10 days(range 4-24). After taking TKM, patient's signs and symptoms were alleviated or resolved and Hb, Hct were increased. Conclusion: After laparoscopic gynecological surgery, patients had taken pain such as abdominal pain, shoulder pain, back pain etc. TKM treatment is effective on the recovery after laparoscopic surgery.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.4
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• pp.269-278
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• 2006

Purpose : The purpose of this study is to report the effect of TKM to both lower extremities weakness after parturition who is diagnosed as Subacute inflammatory demyelinating peripheral neuropathy. Methods : The patient was a 31-year-old woman who couldn't walk at all by herself because of both lower extremities weakness after parturition. The patient was demonstrated as shin-yang-heo, and treated by Pal-mi-ji-hwang-hwan, Acupuncture treatment of Shin-jeong-gyeok, and Moxa treatment of Gwan-won. And the progress of symtoms was evaluated by observation of her walking status, cheking the change of VAS, and inspecting the DITI. Results : The patient could walk by herself. The feeling of both lower extremities weakness improved to VAS 2. The paraesthesia of the soles of feet improved to VAS 4. And the DITI state slightly got better. Conclusion : TKM is expected to have positive effect on puerperium disease especially the symptoms of pain, weakness, paraesthesia, etc.

• Journal of Oriental Medical Thermology
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• v.6 no.1
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• pp.39-48
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• 2008

Purpose: The purpose of this study is to report the effect of TKM to Rhumatitis Arthritis after parturition and show the importance of recuperation after parturation. Methods: The patient was a 33-year-old woman who complains arthralgia, edema and sense of coldness after her third parturation. The patient was treated by Herb medicine, Acupuncture treatment, Moxa treatment and Physical Treatment. And the progress of symtoms was evaluated by cheking the change of VAS, and inspecting the DITI. Results: The Pain and edema have declined. After discharge, she got definite diagnosis as Rhumatitis Arthritis. But We already estimated it from the results of serum test. So we had treated the patience with TKM and we could explain the prognosis. We regulated the pain and edema successfully at the acute phase. And the patient could take care of herself, she didn't play her disease down as a simple symptoms of parturation. Conclusion: TKM is expected to have positive effect on Rheumatic arthritis with quick onset after parturation.

• Journal of Oriental Medical Thermology
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• v.5 no.1
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• pp.14-20
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• 2006

Purpose: The purpose of this study is to report the effect of TKM to both lower extremities weakness after parturition who is diagnosed as Subacute inflammatory demyelinating peripheral neuropathy. Methods: The patient was a 31-year-old woman who couldn't walk at all by herself because of both lower extremities weakness after parturition. The patient was demonstrated as shin-yang-heo, and treated by Pal-mi-ji-hwang-hwan, Acupuncture treatment of Shin-jeong-gyeok, and Moxa treatment of Gwan-won. And the progress of symtoms was evaluated by observation of her walking status, cheking the change of VAS, and inspecting the DITI. Result: The patient could walk by herself. The feeling of both lower extremities weakness improved to VAS 2. The paraesthesia of the soles of feet improved to VAS 4. And the DITI state slightly got better. Conclusion: TKM is expected to have positive effect on puerperium disease especially the symptoms of pain, weakness, paraesthesia, etc.