• Journal of Microbiology and Biotechnology
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• v.17 no.12
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• pp.2076-2080
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• 2007

The endoxylanase (GenBank Access No. U51675) of Bacillus spp. and endoglucanase (GenBank Access No. AY466436) of Trichoderma spp. were separately inserted downstream of the yeast constitutive ADHI promoter, resulting in three different plasmids (pAGX1, pAGX2, and pAGX3) according to the transcription direction of two genes. When the yeast transformants, S. cerevisiae SEY2102 harboring each expression plasmid, were grown on YPD medium, the total activities of the enzymes were approximately 3.01 unit/ml, 3.24 unit/ml, and 7.56 unit/ml for endoxylanase and 0.60 unit/ml, 0.54 unit/ml, and 0.39 unit/ ml for endoglucanase, in the following order: the pAGX1, pAGX2, and pAGX3. More than 70% of the endoxylanase and endoglucanase activities was found in the extracellular media.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.35 no.3
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• pp.263-268
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• 2011

We aimed to develop a non-clogging submersible slurry pump with two blades to replace the conventional vortex pump. To do this, we simulated the effect of parameters such as the blade angle $\beta$ and the blade-length angle $\alpha$ on pump efficiency. We used the commercial codes ANSYS CFX and BladeGen. The results showed that the best blade shape was obtained for $\beta$ = $30^{\circ}$ and that the pump efficiency was proportional to $\alpha$ in the simulated range.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.37 no.2
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• pp.149-154
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• 2013

This study was performed to develop a high efficiency submersible axial pump for concentration wastewater treatment. To do this, we simulated the effect of some parameters such as the axial twist angle of a blade(${\beta}$), the radial twist angle of a blade(${\alpha}$) and the length of a blade (l) on pump efficiency using commercial code, ANSYS CFX and BladeGen. The results showed that the axial twist angle of a blade(${\beta}$) was the most sensible parameter on the pump efficiency. And the pump efficiency had a maximum at ${\beta}=20^{\circ}$, ${\alpha}=110^{\circ}$ and l=240 mm.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.14 no.5
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• pp.2294-2309
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• 2020

In this article, a new Aloha-based tag identification protocol is presented to improve the reading efficiency of the EPC C1 Gen2-based UHF RFID system. Collision detection (CD) plays a vital role in tag identification process which determines the efficiency of anti-collision protocols since most Aloha-based protocols optimize the incoming frame length based on the collisions in current frame. Existing CD methods are ineffective in identifying collision, resulting in a degradation of identification performance. Our proposed algorithm adopts an enhanced CD (ECD) scheme based on the EPC C1 Gen2 standard to optimize identification performance. The ECD method can realize timely and effective CD by detecting the pulse width of the randomly sent by tags. According to the ECD, the reader detects the slot distribution and predicts tag cardinality in every collision slot. The tags involved in each collision slot are identified by independently assigned sub-frames. A large number of numerical results show that the proposed solution is superior to other existing anti-collision protocols in various performance evaluation metrics.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2001.05a
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• pp.307-310
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• 2001

A parameterized complex-number multiplier (PCMUL) core IP (Intellectual Property), which can be used as an essential arithmetic unit in baseband signal processing of digital communication systems, is described. The bit-width of the multiplier is parameterized in the range of 8-b~24-b and is user-selectable in 2-b step. The PCMUL_GEN, a core generator with GUI, generates VHDL code of a CMUL core for a specified bit-width. The IP is based on redundant binary (RB) arithmetic and a new radix4 Booth encoding/decoding scheme proposed in this paper. It results in a simplified internal structure, as well as high-speed, low-power, and area-efficient implementation. The designed IP was verified using Xilinx FPGA board.

• Journal of Life Science
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• v.21 no.2
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• pp.273-278
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• 2011

Plants which had been classified to the Scrophulariaceae of the Lamiales were recently reclassified. Many of them were moved to the other families of Lamiales according to the DNA sequences of the plastid DNA. Among those, Melampyrum roseum, Phtheirospermum japonicum, Pseudolysimachion undulata, Lindernia crustacea and Mazus pumilus were chosen for phylogenetic analyses. DNA sequences of 18S rRNA gene and ITS1 of those plants were determined and deposited into GenBank (accession numbers GU359046, GU359047, GU359048, GU359049, GU359050, respectively). Analyses of those DNA sequences confirmed the current classification done on the basis of the plastid DNA sequences of Melampyrum roseum, Phtheirospermum japonicum and Pseudolysimachion undulata. However, it was not possible to classify Mazus pumilus and Lindernia crustacea due to discrepancies of analyses data.

• The Journal of Asian Finance, Economics and Business
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• v.8 no.12
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• pp.509-518
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• 2021

Amid the Covid pandemic surge last year, customers and businesses started embracing digital transactions. Digital banking, as an answer to the cash-less-contact-less way of doing financial transactions, was highly convenient during the pandemic, especially in the Philippines. This position examines the Gen Y and Gen Z in the Philippine case and its willingness to encourage digitalization of the financial sector. This study aimed at evaluating the intention to use digital banking services using the factors (1) ease of use; (2) perceived usefulness; (3) perceived risk; (4) trust; (5) convenience. A total of 226 respondents were selected by random sampling method and linear regression was used to analyse the collected data. Analysis of the results show that the components (1) perceived ease of use; (2) perceived usefulness; (3) perceived risk; (4) trust; (5) convenience had a significant effect on intention to use digital banking while the demographics - gender, age, monthly income, and educational attainment do not have any significance on the intention to use digital banking. The relevance of the study can be used for marketing and financial strategies to increase the intention to use digital banking and to contribute to the enhancement of technology acceptance concerning digital banking.

• Korean Journal of Environmental Biology
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• v.41 no.2
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• pp.145-166
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• 2023

Parrots have been threatened by global trade to meet their high demand as pets. Controlling parrot trade is essential because parrots play a vital role in the ecosystem. Accurate species identification is crucial for controlling parrot trade. Parrots have been traded as eggs due to their advantages of lower mortality rates and more accessible transport than live parrots. A molecular method is required to identify parrot eggs because it is difficult to perform identification using morphological features. In this study, DNAs were obtained from 43 unidentified parrot eggs using a non-destructive sampling method. Partial cytochrome b (CYTB) gene was then successfully amplified using polymerase chain reaction (PCR) and sequenced. Sequences newly obtained in the present study were compared to those available in the GenBank by database searching. In addition, phylogenetic analysis was conducted to identify species using available sequences in GenBank along with sequences reported in previous studies. Finally, the 43 parrot eggs were successfully identified as seven species belonging to two families and seven genera. This non-destructive sampling method for obtaining DNA and molecular identification might help control the trade of parrot eggs and prevent their illegal trade.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.143.3-144
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• 2003

A Korean isolate of Pepper mild mottle virus (PMMoV-Kr) was isolated from a diseased pepper crop in Chunchon, Korea. The isolate was biologically purified on Nicoticaa tabacum cv. Xanthi-nc by successive single local transfer steps, and propagated on N. tabacum cv. Samsun. PMMoV-Kr could systemically infect on N. glauca, N. benthmiana, N. occidentalis and Lycopersicon esculentum, which is typical of known isolates of PMMoV. PMMoV-Kr belongs to the pathotype P1,2 based on pepper-tobamoviral indicator experiments; Capsicn chinone harboring L3 gene revealed resistant (necrotic local lesion on inoculated leaf, HR) whereas L+, L1 and L2 pepper plants expressed susceptible reactions of mosaic systemic symptoms for the isolate. To confirm the pathology and delineate symptom determinant of the isolate, full-length cDNAs of PMMoV-Kr were amplified by RT-PCR with a primer set corresponding to the 5'- and 3'-ends of PMMoV. The RT-PCR molecules amplified from genome RNA of the isolate was cloned into the pUC18 vector. Full-length cDNA clones constructed under the control of the T7 RNA promoter could be successfully transcribed to produce in vitro transcript RNA. Infectivity of the capped transcripts and its progeny virus was verified by Western blot and RT-PCR analyses.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.146.2-147
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• 2003

Two Korean isolates of Alfalfa mosaic virus (AHV-AZ, AMV-KR) were isolated from azuki bean and potato plants, respectively, and their pathologies were confirmed on some susceptible host plants including pepper, tobacco and red bean plants. Full length cDNAs to RNA1, RNA2 and RNA3 of the two Korean strains were amplified using the long-template reverse transcription (RT)-polymerase chain reaction (PCR) method. RT-PCR products covering entire regions for the three AMV genome RNAs were cloned. RNA transcripts were synthesized in vitro from each clones using T7 RNA polymerase and infectivity test was peformed in 9 reassortment sets of transcripts. All the combinations of reassorted transcripts were found to be infectious when inoculated onto Nicotiana benthamiana plants, and were not distinguishable to those of wild types. The full-length cDNA clones that were confirmed infectious were sequenced their nucleotide sequences. We will discuss sequence analysis of the two Korean isolates of AMV genomic RNA3 and compare reported foreign isolates of AMV.