Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Constitutive Coexpression of Bacillus Endoxylanase and Trichoderma Endoglucanase Genes in Saccharomyces cerevisiae

  • Lee, Jae-Hyung (Department of Biomaterial Control (BK21 Program), Dong-Eui University) ;
  • Lim, Myung-Ye (Department of Biomaterial Control (BK21 Program), Dong-Eui University) ;
  • Kim, Mi-Jin (Department of Biomaterial Control (BK21 Program), Dong-Eui University) ;
  • Heo, Sun-Yeon (Insect Research Center, Korea Research Institute of Biotechnology & Bioengineering) ;
  • Seo, Jin-Ho (Department of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Yeon-Hee (Department of Biotechnology and Bioengineering, Dong-Eui University) ;
  • Nam, Soo-Wan (Department of Biomaterial Control (BK21 Program), Dong-Eui University)
  • Published : 2007.12.31
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Abstract

The endoxylanase (GenBank Access No. U51675) of Bacillus spp. and endoglucanase (GenBank Access No. AY466436) of Trichoderma spp. were separately inserted downstream of the yeast constitutive ADHI promoter, resulting in three different plasmids (pAGX1, pAGX2, and pAGX3) according to the transcription direction of two genes. When the yeast transformants, S. cerevisiae SEY2102 harboring each expression plasmid, were grown on YPD medium, the total activities of the enzymes were approximately 3.01 unit/ml, 3.24 unit/ml, and 7.56 unit/ml for endoxylanase and 0.60 unit/ml, 0.54 unit/ml, and 0.39 unit/ ml for endoglucanase, in the following order: the pAGX1, pAGX2, and pAGX3. More than 70% of the endoxylanase and endoglucanase activities was found in the extracellular media.

Keywords

References

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