• Proceedings of the Ginseng society Conference
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• 2002.10a
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• pp.66-83
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• 2002

Recently, we have provided evidence that ginsenosides, the active components of Panax ginseng, utilize pertussis toxin (PTX)-insensitive $G{\alpha}_{q/11}-phospholipase\;C-{\beta}3(PLC-{\beta}3)$ signal transduction pathway for the enhancement of $Ca^{2+}-activated\;Cl^{-}$ current in the Xenopus oocyte (British J. Pharmacol. 132, 641-647, 2001; JBC 276, 48797-48802, 2001). Other investigators have shown that stimulation of receptors linked to $G{\alpha}-PLC$ pathway inhibits the activity of G proteincoupled inwardly rectifying $K^+$ (GIRK) channel. In the present study, we sought to determine whether ginsenosides influenced the activity of GIRK 1 and GIRK 4 (GIRK 1/4) channels expressed in the Xenopus oocyte, and if so, the underlying signal transduction mechanism. In oocyte injected with GIRK 1/4 channel cRNAs, bath-applied ginsenosides inhibited high potassium (HK) solution-elicited GIRK current $(EC_{50}:4.9{\pm}4.3\;{\mu}g/ml).$ Pretreatment of the oocyte with PTX reduced the HK solution-elicited GIRK current by $49\%,$ but it did not alter the inhibitory ginsenoside effect on GIRK current. Prior intraoocyte injection of cRNA(s) coding $G{\alpha}_q,\;G{\alpha}_{11}\;or\;G{\alpha}_q/G{\alpha}_{11},\;but\;not\;G{\alpha}_{i2}\;or\;G{\alpha}_{oA}$ attenuated the inhibitory ginsenoside effect. Injection of cRNAs coding $G{\beta}_{1{\gamma}2}$ also attenuated the ginsenoside effect. Similarly, injection of the cRNAs coding regulators of G protein signaling 1, 2 and 4 (RGS1, RGS2 and RGS4), which interact with $G{\alpha}_i\;and/or\;G{\alpha}_{q/11}$ and stimulates the hydrolysis of GTP to GDP in active GTP-bound $G{\alpha}$ subunit, resulted in a significant reduction of ginsenoside effect on GIRK current. Preincubation of GIRK channel-expressing oocyte in PLC inhibitor (U73122) or protein kinase C (PKC) inhibitor (staurosporine or chelerythrine) blocked the inhibitory ginsenoside effect on GIRK current. On the other hand, intraoocyte injection of BAPTA, a free $Ca^{2+}$ chelator, had no significant effect on the ginsenoside action. Taken together, these results suggest that ginsenosides inhibit the activity of GIRK 1/4 channel expressed in the Xenopus oocyte through a PTX-insensitive and $G{\alpha}_{q/11}$-,PLC-and PKC-mediated signal transduction pathway.

• BMB Reports
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• v.45 no.3
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• pp.153-158
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• 2012

Geft is a guanine nucleotide exchange factor, which can specifically activate Rho family of small GTPase by catalyzing the exchange of bound GDP for GTP. Geft is highly expressed in the excitable tissue as heart and skeletal muscle and plays important roles in many cellular processes, such as cell proliferation, migration, and cell fate decision. However, the in vivo role of Geft remains unknown. Here, we generated a Geft conditional knockout mouse by flanking exons 5-17 of Geft with loxP sites. Cre-mediated deletion of the Geft gene in heart using Mef2c-Cre transgenic mice resulted in a dramatic decrease of Geft expression. Geft knockout mice develop normally and exhibit no discernable phenotype, suggesting Geft is dispensable for the development of the second heart field in mouse. The Geft conditional knockout mouse will be a valuable genetic tool for uncovering the in vivo roles of Geft during development and in adult homeostasis.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.5
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• pp.685-695
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• 2011

Satellite cells are skeletal muscle progenitor/stem cells that reside between the basal lamina and plasma membranes of skeletal fibers in vivo. These cells can give rise to both myogenic and adipogenic cells. Given the possible role for differentiation of satellite cells into adipocytes in marbling and in some pathological disorders like sarcopenia, knowledge of the proteins involved in such process remains obscure. Using two-dimensional polyacrylamide gel electrophoresis coupled with mass spectrometry, we investigated the proteins that are differentially expressed during adipogenic differentiation of satellite cells from bovine longissimus muscle. Our proteome mapping strategy to identify the differentially expressed intracellular proteins during adipogenic differentiation revealed a total of 25 different proteins. The proteins up-regulated during adipogenic differentiation of satellite cells like Cathepsin H precursor, Retinal dehydrogenase 1, Enoyl-CoA hydratase, Ubiquinol-cytochrome-c reductase, T-complex protein 1 subunit beta and ATP synthase D chain were found to be associated with lipid metabolism. The down-regulated proteins like LIM protein, annexin proteins, cofilin-1, Rho GDP-dissociation inhibitor 1 and septin-2, identified in the present study were found to be associated with myogenesis. These results clearly demonstrate that the adipogenic conversion of muscle satellite cells is associated with the up-regulated and down-regulated proteins involved in adipogenesis and myogenesis respectively.

• Advances in environmental research
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• v.6 no.2
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• pp.127-137
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• 2017

Cotton is the major fiber crop in Pakistan that accounts for 2% of total national gross domestic product (GDP). After picking of cotton, the dry stalks are major organic waste that has no fate except burning to cook food in villages. Present research focuses use of cotton stalks as feedstock for biochar production, its characterization and effects on soil characteristics. Dry cotton stalks collected from agricultural field of Bahauddin Zakariya University, Multan, Pakistan were combusted under anaerobic conditions at $450^{\circ}C$. The physicochemical analysis of biochar and cotton stalks show higher values of % total carbon, phosphorus and potassium concentrations in biochar as compared to cotton stalks. The concentration of nitrogen was decreased in biochar. Similarly biochar had greater values of fixed carbon that suggest its role for carbon sequestration and as a soil amendment. The fourier transformation infrared spectroscopic spectra (FTIR) of cotton stalks and biochar exposed more acidic groups in biochar as compared to cotton stalks. The newly developed functional groups in biochar have vital role in increasing surface properties, cation exchange capacity, and water holding capacity, and are responsible for heavy metal remediation in contaminated soil. In a further test, results show increase in the water holding capacity and nutrient retention by a sandy soil amended with biochar. It is concluded that cotton stalks can be effectively used to prepare biochar.

• Journal of Microbiology and Biotechnology
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• v.28 no.8
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• pp.1293-1298
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• 2018

Phosphomannomutase (ManB) converts mannose-6-phosphate (M-6-P) to mannose-1-phosphate (M-1-P), which is a key metabolic precursor for the production of GDP-D-mannose used for production of glycoconjugates and post-translational modification of proteins. The aim of this study was to express the manB gene from Escherichia coli in Lactococcus lactis subsp. cremoris NZ9000 and to characterize the encoded enzyme. The manB gene from E. coli K12, of 1,371 bp and encoding 457 amino acids (52 kDa), was cloned and overexpressed in L. lactis NZ9000 using the nisin-controlled expression system. The enzyme was purified by Ni-NTA column chromatography and exhibited a specific activity of 5.34 units/mg, significantly higher than that of other previously reported ManB enzymes. The pH and temperature optima were 8.0 and $50^{\circ}C$, respectively. Interestingly, the ManB used in this study had two substrate specificity for both mannose-1-phosphate and glucose-1-phosphate, and the specific activity for glucose-1-phosphate was 3.76 units/mg showing 70% relative activity to that of mannose-1-phosphate. This is the first study on heterologous expression and characterization of ManB in lactic acid bacteria. The ManB expression system constructed in this study canbe used to synthesize rare sugars or glycoconjugates.

• Journal of Korean Society of Rural Planning
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• v.29 no.3
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• pp.91-97
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• 2023

The purpose of this research is to apply the regional development attractiveness of the national level determined in the previous study to the city and county level of Chungcheongnam-do. We verified results with the population change of the floating population data. In order to measure regional development attractiveness in 2020, Chungcheongnam-do's integrated air environment index and per capita gross regional product were gathered. Population movement data over the past five years have been used to analyze population changes in the floating population data. Regional development attractiveness depended on the data of GDP per capita, which had a large difference between the maximum and minimum values. The rate of increase or decrease in population change by city and county in Chungcheongnam-do over the past five years has changed significantly since 2021 and characteristics of each group were grouped into four groups. Based on the environment and economic feasibility of the region, it can be the starting point for a new analysis of Korea's regional development projects and the selection of target sites. Policy suggestions can also be made in spatial plans such as short-term comprehensive plans, long-term comprehensive plans, and development plans. It can be a limit of this research that regional development attractiveness was determined by the relatively large per capita gross domestic product. It is necessary to further develop regional development attractiveness by closely investigating the characteristics of the region, social problems, and emissions of environmentally harmful substances.

• International Area Studies Review
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• v.20 no.1
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• pp.105-125
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• 2016

The expansionary monetary policy was practiced after 2001 in Japan to treat the deflation spiral, and reduced only the nominal interest rates and domestic household demand. One of the most serious factors for this failure was the change of private sector's expectancy. This paper has studied the effect of Japanese monetary policy in 21c., with empirical research based on a renewed macroeconomic model and the VAR. The empirical analysis shows that the effect of monetary policy on the national income during 2001.01-2015.03 is weaker than that of 1985.01-1994.04. Money volume has a diminutive effect on the growth of GDP within a short term after 2001. The change in the expectations of the private sectors might have been the cause of ineffectiveness of the expansive monetary policy. Economic agents learned from the past Japanese financial crisis that an expansive monetary policy increased the inflation rate and caused the 'bubbles to burst' afterwards. The VAR analysis says that the effectiveness of monetary policy on the economic depression declined over the past 20 years and the expansion of money volume has no influence on exchange rate and net export. This means that the expansive monetary policy lost its effect on net export and national income steadily. Monetary policy makers have to recognize this fact, and to consider another anti-cycle political instrument, i.e. the fiscal policy with government debt.

• Journal of Energy Engineering
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• v.24 no.2
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• pp.167-173
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• 2015

This paper attempts to estimate the electricity demand function in Korea with quarterly data of average temperature, GDP and electricity price over the period 2005-2013. We apply lagged dependent variable model and ordinary least square method as a robust approach to estimating the parameters of the electricity demand function. The results show that short-run price and income elasticities of the electricity demand are estimated to be -0.569 and 0.631, respectively. They are statistically significant at the 1% level. Moreover, long-run income and price elasticities are estimated to be 1.589 and -1.433, respectively Both of results reveal that the demand for electricity is price- and income-elastic in the long-run. The relationship between electricity consumption and temperature is supported by many of references as a U-shaped relationship, and the base temperature of electricity demand is about $15.2^{\circ}C$. It is shown that power of explanation and goodness-of-fit statistics are improved in the use of the lagged dependent variable model rather than conventional model.

• 전기의세계
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• v.18 no.1
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• pp.33-37
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• 1969

한전은 1968년 11월 3일 오전 10시 04분을 기하여 154KV 전계통의 직접접지방식 전환을 단행하였다. 종전의 P.C(소고선륜) 중성점접지방식을 직접접지방식으로 전환한것으로서 전력사상 특기 할 만한 근대화사업이며 다년간을 두고 추진해온 중요과제였다. 전력계통의 확대와 복잡화는 선진국가에서도 실시하고 있는 직접접지방식의 채택을 불가변하게 하였고 또한 1차 AID송배전차관도 이의 채택을 조건부로 승인되었던 것으로서 1968년 이후에 건설되는 송변전기기는 직접접지계에서만 운전할 수 있는 절연Level 650KV급이 도입되어 부산화력발전소 3호기가 준공되는 1968년 10월말까지는 직접접지전환이 반드시 이루어져야 하는 실정에 놓여 있었다. 그런데 직접접지방식의 단점인 인접통신선에 미치는 유도장해를 해결하는 문제가 다년간을 두고 진지하게 검토되어 왔으나 해결이 늦어지고 있었다. 사유는 154KV 계통에 인근된 통신선이라면 체신부, 내무부, 교통부, 국방부등 여러기관의 것이 있는데, 유도장해보안방법과 유도보상비문제에 대하여 전력측(상공부, 한전)과 통신측(상기의 체신부등)의 견해차가 해소되지 않기 때문이었다. 그것이 1968년 5월에 와서 전력.통신쌍방이 범국가적입장에서 제반애로를 무릅쓰고 최소한의 투자로 가능한 범위내의 보안책으로서 우선 Arrester 보안방식을 채택하기로 합의되어 경제장관회의를 거쳐 시공하기에 이른것이다. 이 란을 빌려 이 사업의 필요성과 경위및 통신선유도장해방지를 위한 보안방식내용을 간단히 소개함으로써 앞으로 이 분야의 항구적인 유도대책연구에 다소나마 참고가 된다면 다행으로 생각하겠다.면서 예측강우의 질이 저하되기 시작하였으나 QPM을 합성함으로써 생산한 BQPF는 보다 신뢰성있고 양호한 결과를 얻을 수 있었다. 이러한 결과들은 향후 정량적 분포형강우 예측을 이용한 실시간 홍수유출 예측시 댐운영자는 리드타임(홍수선행시간)을 충분히 확보함으로서 안정적이고 예측 가능한 홍수조절을 하는데 도움을 줄 수 있을 것으로 기대된다. 이와 같이 다양한 단기저수지 유입량의 예측정보 제공으로 다목적댐 저수지 운영모형의 효용성을 제고하여 향후 실제 저수지 유입량 예측에 이용함으로써 저수지 단기운영효율 개선에 기여할 수 있을 것으로 사료된다.다. 이것은 여름철 강수량이 증가하고, 호우발생빈도, 특히 8월의 강수일수가 증가하고 있다는 것과 밀접한 관련이 있다. 여름과 가을에 우리나라에 영향을 미치는 태풍의 수는 뚜렷한 추세를 보이지 않으나, 2002년 루사, 2003년 매미로 인하여 각각 6조원, 4조원 이상의 막대한 피해가 발생하였다. 태풍에 의한 피해액은 GDP 대비 약 0.9%(태풍 루사)로 최근 경제상장률과 비교해 보면, 상당한 비율을 차지한다. 우리나라에 영향을 미치는 태풍은 연근해의 해수면 온도가 높아지면 세기가 강해질 가능성이 높다. 폭설과 한파일수도 평년대비 최근 10년 감소하였고 일최저기온이 영하 $10^{\circ}C$ 이하인 날도 연간 발생일수가 감소하였다. 최근 10년간 우리나라 기후의 변화특성은 기온상승과 더불어 서리종료일이 앞당겨지고 열대야가 증가하고 폭설, 한파, 겨울철 일최저기온 영하 10도 이하인 날의 감소 등이 나타나고, 여름철 재해의 원인인 호우일수는 증가하는 추세이다.

• Biomolecules & Therapeutics
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• v.16 no.3
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• pp.197-202
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• 2008

In our previous publication we compared the gene expression profiles on hepatotoxicants exposure to assess the comparability between in vivo and in vitro test systems. We investigated global gene expression from both mouse liver and mouse hepatic cell line treated with thioacetamide (TAA) and identified several common genes. In this study, we selected genes to validate them as potential biomarkers for hepatotoxicity on the relevance of in vitro and in vivo system. Three up-regulated, aquaporin 8 (Aqp8), glutathione peroxidase 1 (Gpx1), succinate-CoA ligase, GDP-forming, alpha subunit (Suclg1) and two down-regulated, DnaJ (Hsp40) homolog subfamily C member 5 (Dnajc5) and tumor protein D52 (Tpd52) genes were tested for their effects in vitro. For characterization of gene function, short interfering RNA (siRNA) for each gene was synthesized and transfected in mouse hepatic cell line, BNL CL.2. Cell viability, mRNA expression level and morphological alterations were investigated. We confirmed siRNA transfection against selected five genes induced down-regulation of respective mRNA expression. siRNA transfection in general decreased cell viability in different degrees and induced morphological changes such as membrane thickening and alterations of intracellular structures. This suggests that these genes could be associated with TAA-induced toxicity. Furthermore, these genes may be used in the investigation of hepatotoxicity for better understanding of its mechanism.