• Korean Journal of Medicinal Crop Science
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• v.12 no.1
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• pp.24-30
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• 2004

The immune activities of Ganoderma lucidum Mycelium added garlic extracts (GAM), Ganoderma lucidum Mycelium (GM), garlic extracts (GS) and standard $({\beta}-glucan)$ were compared. GAM enhanced the growth of human immune T cell up to $1.25{\sim}1.46$ times, compared to control group. GAM showed relatively lower cytotoxicity in using normal human lung cell, while GAM showed the most potent inhibitory effect on the human lung carcinoma, compared to GM and GS. The selectivity of GAM was also higher than that of GM and GS. GAM increased the secretion of cytokines, IL-6 and TNF- from human B cell as well as the growth of human immune cells. It can imply that GAM has higher immune activity than GM or GS.

• The Journal of Korean Medical History
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• v.23 no.2
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• pp.43-46
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• 2010

Dong-Eui-Bo-Gam is a general medical literature, writing by Korea physician Xujun, who makes reference of Chinese medical literatures, Taoist literatures, historical records, Confucian literatures and so forth prior Ming Dynasty. It coveres many fields, such as medical theory, etiology, pulse theory, herb, prescription, internal medicine, surgery, gynecology, pediatrics, acupuncture, regimen, YunQi and so forth. Dong-Eui-Bo-Gam combines medical science and many others, using clustering arrangements, fully reflects Xujun's academic thoughts, and his rich clinical experiences.

• Journal of Physiology & Pathology in Korean Medicine
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• v.24 no.5
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• pp.727-736
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• 2010

This study is about researching mechanistic pattern identification of disease for DongEuiBoGam NaeGyungPyen by analysing with pattern identification of modern Traditional Korean medical patholgy as more logical, systematic and standardized theory. Disease pattern mechanisms of uterine, urine and excrements parts of DongEuiBoGam NaeGyun gPyen in NaeGyungPyen of DongEuiBoGam are these. Menstrual irregularities in DongEuiBoGam can be classified flui d-humor depletion, blood deficiency, qi deficiency, qi stagnation, qi stagnation complicated by heat, blood stasis, blood deficiency complicated by heat, syndrome of heat entering blood chamber, syndrome of cold entering blood chamber. The disease pattern of abdominal pain after menstruation in DongEuiBoGam is blood deficiency complicated by heat, and a dysmenorrhea represents blood stasis with heat, fluid-humor deficiency. Advanced menstruation represent dual heat of the qi and blood, delayed menstruation is blood deficiency. The disease pattern of inhibited urination in DongEuiBoGam can be classified deficiency heat pattern of kidney yin deficiency(yin deficiency with effulgent fire), kidney qi deficiency, yin deficiency with yang hyperactivity, fluid-humor depletion, spleen-stomach dual deficiency, and excess he at pattern of bladder excess heat. The disease pattern of urinary incontinence in DongEuiBoGam can be classified deficiency pattern of kidney-bladder qi deficiency, consumptive disease, lung qi deficiency, kidney yin deficiency(yin deficiency with effulgent fire), kidney yang deficiency and excess pattern of lower energizer blood amassment, bladder excess heat. And most of them are deficiency from deficiency-excess Pattern Identification. The disease pattern of diarrhea in DongEuiBoGam can be classified deficiency pattern of qi deficiency, qi fall, spleen yang deficiency, kidney yang deficiency and so on and excess pattern of wind-cold-summerheat-dampness-fire, phlegm-fluid retention, dietary irregularities, qi movement stagnation. And most of them are deficiency from deficiency-excess Pattern Identification. Like these, this study identify pattern of disease in DongEuiBoGam by mechanism of disease theory.

• Journal of the Korean Institute of Oriental Medical Informatics
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• v.15 no.2
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• pp.7-56
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• 2009

Objective: The purpose of this study is to compare the original texts with quotations in 16 Kinds of Cough Part in Haesu Chapter of Dong-Yi-Bo-Gam and to find out the ideas of Huh Jun(許浚, 1546-1615; the author of Dong-Yi-Bo-Gam) in there. Methods: I compared the original texts with quotations in 16 Kinds of Cough Part in Haesu Chapter of Dong-Yi-Bo-Gam. Results: 1. There is only one quoted sentence which perfectly matches with original text in 16 Kinds of Cough Part in Haesu Chapter of Dong-Yi-Bo-Gam. The other sentences are all modified while they are quoted by Huh Jun, at least one word. 2. The arrangement order of 'medical effect', 'consisting medicines and their dosages' and 'doctrine in application' were rearranged following the form of Dong-Yi-Bo-Gam when being quoted. 3. In cases of reciting the text, Huh Jun tries to clarify the original source of the context. However, instead of using original quotations he recited rephrased quotes from other sources. 4. Huh Jun cites from not only cough parts of other texts but also asthma(喘症) or heat(積熱) parts. 5. Titles of original text books are recorded in the end of all sentences of Dong-Yi-Bo-Gam, but there are a few wrong titles recorded. Conclusion: In consideration of the above-mentioned, the Dong-Yi-Bo-Gam is not the mere collection of various Oriental Medical books, but the Classic of Oriental Medicine to hold Huh Jun's own opinion.

• Parasites, Hosts and Diseases
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• v.51 no.2
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• pp.147-154
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• 2013

To control coccidiosis without using prophylactic medications, a DNA vaccine targeting the gametophyte antigen Gam56 from Eimeria maxima in chickens was constructed, and the immunogenicity and protective effects were evaluated. The ORF of Gam56 gene was cloned into an eukaryotic expression vector pcDNA3.1(zeo)+. Expression of Gam56 protein in COS-7 cells transfected with recombinant plasmid pcDNA-Gam56 was confirmed by indirect immunofluorescence assay. The DNA vaccine was injected intramuscularly to yellow feathered broilers of 1-week old at 3 dosages (25, 50, and $100{\mu}g/chick$). Injection was repeated once 1 week later. One week after the second injection, birds were challenged orally with $5{\times}10^4$ sporulated oocysts of E. maxima, then weighed and killed at day 8 post challenge. Blood samples were collected and examined for specific peripheral blood lymphocyte proliferation activity and serum antibody levels. Compared with control groups, the administration of pcDNA-Gam56 vaccine markedly increased the lymphocyte proliferation activity (P<0.05) at day 7 and 14 after the first immunization. The level of lymphocyte proliferation started to decrease on day 21 after the first immunization. A similar trend was seen in specific antibody levels. Among the 3 pcDNA-Gam56 immunized groups, the median dosage group displayed the highest lymphocyte proliferation and antibody levels (P<0.05). The median dosage group had the greatest relative body weight gain (89.7%), and the greatest oocyst shedding reduction (53.7%). These results indicate that median dosage of DNA vaccine had good immunogenicity and immune protection effects, and may be used in field applications for coccidiosis control.

• Journal of Microbiology and Biotechnology
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• v.25 no.1
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• pp.33-43
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• 2015

GAM-1 and GAM-2, two themostable glucoamylases from Aspergillus niger B-30, possess different molecular masses, glycosylation, and thermal stability. In the present study, the effects of additives on the thermal inactivation of GAM-1 and GAM-2 were investigated. The half-lives of GAM-1 and GAM-2 at 70℃ were 45 and 216 min, respectively. Data obtained from fluorescence spectroscopy, circular dichroism spectroscopy, UV absorption spectroscopy, and dynamic light scattering demonstrated that during the thermal inactivation progress, combined with the loss of the helical structure and a majority of the tertiary structure, tryptophan residues were partially exposed and further led to glucoamylases aggregating. The thermal stability of GAM-1 and GAM-2 was largely improved in the presence of sorbitol and trehalose. Results from spectroscopy and Native-PAGE confirmed that sorbitol and trehalose maintained the native state of glucoamylases and prevented their thermal aggregation. The loss of hydrophobic bonding and helical structure was responsible for the decrease of glucoamylase activity. Additionally, sorbitol and trehalose significantly increased the substrate affinity and catalytic efficiency of the two glucoamylases. Our results display an insight into the thermal inactivation of glucoamylases and provide an important base for industrial applications of the thermally stable glucoamylases.

• The Journal of Korean Medical History
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• v.17 no.1
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• pp.119-141
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• 2004

DongUiBoGam(東醫寶鑑) is Joseon's classic medical text that has great significance in the medical history of Korea. However, the context of the treatments of accupuncuret&moxibustion in the book are hardly studied. This study is for the purpose of examining the treatments of accupuncuret&moxibustion in DongUiBoGam(東醫寶鑑), and it starts with studying the accupuncuret&moxibustion part of DongUiBoGam(東醫寶鑑)'s NaeGyeong Chapter(內景篇) for starters. Through this study, the following could be known. 1. In DongUiBoGam(東醫寶鑑)'s NaeGyeong Chapter(內景篇), it is written that it quoted many various books, but actually, most of the parts are quoted from LouYing(樓英)'s YiXueGangMu (醫學綱目). 2. LouYing(樓英)'s YiXueGangMu(醫學綱目) is a work of the Ming Dynasty, which is when China's study of accupuncure&moxibustion was completed and many books on accupuncure&moxibustion were published. 3. However, in DongUiBoGam(東醫寶鑑), it mainly quotes from YiXueGangMu(醫學綱目), instead of these professional acupuncture&moxibustion texts. This is because this book is a comprehensive medical text with various medical theories, which share the same flow with DongUiBoGam(東醫寶鑑), and at the same time, it organized the treatments of accupuncure&moxibustion in a big scale in various ways.

• Journal of Physiology & Pathology in Korean Medicine
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• v.16 no.2
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• pp.209-214
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• 2002

At this paper, I classified ‘tetanus’ in 『DongEuiBoGam』 and studied the concept, causes, symptoms, pathological mechanisms of that disease and then I had a new understanding that concept of tetanus in 『DongEuiBoGam』 is different with concept of tetanus in Western Medicine. In the mean time, I investigated the classification in 「Classification of Korean Standard Cause of Death(Oriental Medicine)」 (1995, The Korean Economic Planning Board), and concluded the concept of tetanus in "DongEuiBoGam".

• Korean Journal of Acupuncture
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• v.20 no.1
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• pp.91-101
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• 2003

Object of this study is as follows. We check on obesity mentioned in and consider the results with an analysis. And then, We offer Obesity's Acupuncture & Moxibustion Therapy with a base by the results and We confirmed the follows by the results of this study. tells that obesity reduces a life and causes a disease. said that Acupuncture & Moxibustion Therapy was effective on a treatment of Obesity.

• Journal of Haehwa Medicine
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• v.14 no.1
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• pp.129-140
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• 2005

1. In Dong-Eui-Bo-Gam, Ondamtng & its Gagambang are applied for the sudden palpitation, epilepsy, the insomnia of exhaustion and an acute convulsion which are caused by the asthenia of heart and gall bladder(心膽虛怯). 2. In Dong-Eui-Bo-Gam, Guibitang & its Gagambang are applied for amnesia, amenorrhea, upper abdominal pain, the itching sign the pubic region, the hernia of the vulva which are caused by the asthenia of heart and spleen(心脾兩虛). 3. In Dong-Eui-Bo-Gam, Soyosan & its Gagambang are applied for epilepsy, the hemorrhage in time of coughing, the intermittent fever, metrorrhagia, various woman's diseases which are caused by the depression of vital energy of liver(肝氣鬱結).