• 생물정신의학
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• 제12권1호
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• pp.13-19
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• 2005

Objective:The purposes of this study were to investigate heart rate variability(HRV) in patients with generalized anxiety disorder(GAD) compared with major depressive disorder in Korea. Methods:Fifty-six GAD patients(20 male and 36 female) was classified into their comorbid psychiatric illness. Among them, Twenty-five patients(10 male and 15 female) who do not have any psychiatric comorbidity were compared with 30 major depressive disorder patients(12 male and 18 female). Clinical symptoms, HRV and MMPI were analysed between two group. Results:Comorbid psychiatric illnesses of GAD were ranked into no diagnosis(44.6%), MDD(32.1%), panic disorder(10.7%), social phobia(5.3%), PTSD(1.7%), OCD(1.7%), MDD+panic disorder(1.7%) and MDD+specific phobia(1.7%). GAD patients showed low functioning in HRV, but degree of decreasing HRV is not so severe compared with MDD patient. Balance of sympathetic and parasympathetic nerve tone is more severely impaired in GAD patients compared with MDD patient. The score of MMPI did not reveal any differences between two groups. Conclusions:The result showed that HRV can differenciate GAD and MDD patients. GAD patients could show decreased HRV functioning, less than MDD patients. But autonomic imbalance could be more severe in GAD than MDD patients.

• BMB Reports
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• 제38권5호
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• pp.595-601
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• 2005

In this study, we have isolated a rice (Oryza sativa L.) glutamate decarboxylase (RicGAD) clone from a root cDNA library, using a partial Arabidopsis thaliana GAD gene as a probe. The rice root cDNA library was constructed with mRNA, which had been derived from the roots of rice seedlings subjected to phosphorus deprivation. Nucleotide sequence analysis indicated that the RicGAD clone was 1,712 bp long, and harbors a complete open reading frame of 505 amino acids. The 505 amino acid sequence deduced from this RicGAD clone exhibited 67.7% and 61.9% identity with OsGAD1 (AB056060) and OsGAD2 (AB056061) in the database, respectively. The 505 amino acid sequence also exhibited 62.9, 64.1, and 64.2% identity to Arabidopsis GAD (U9937), Nicotiana tabacum GAD (AF020425), and Petunia hybrida GAD (L16797), respectively. The RicGAD was found to possess a highly conserved tryptophan residue, but lacks the lysine cluster at the C-proximal position, as well as other stretches of positively charged residues. The GAD sequence was expressed heterologously using the high copy number plasmid, pVUCH. Our activation analysis revealed that the maximal activation of the RicGAD occurred in the presence of both $Ca^{2+}$ and calmodulin. The GAD-encoded 56~58 kDa protein was identified via Western blot analysis, using an anti-GAD monoclonal antibody. The results of our RT-PCR analyses revealed that RicGAD is expressed predominantly in rice roots obtained from rice seedlings grown under phosphorus deprivation conditions, and in non-germinated brown rice, which is known to have a limited phosphorus bioavailability. These results indicate that RicGAD is a $Ca^{2+}$/calmodulin-dependent enzyme, and that RicGAD is expressed primarily under phosphate deprivation conditions.

• Preventive Nutrition and Food Science
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• 제9권4호
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• pp.324-329
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• 2004

In order to investigate the molecular mechanism of $\gamma$-aminobutyric acid (GABA) production in lactic acid bacteria, we cloned a glutamate decarboxylase (GAD) gene from Lactobacillus plantarum using polymerase chain reaction (PCR). One PCR product DNA was obtained and inserted into a TA cloning vector with a T7 promoter. The recombinant plasmid was used to transform E. coli. The insertion of the product was con­firmed by EcoRI digestion of the plasmid purified from the transformed E. coli. Nucleotide sequence analysis showed that the insert is a full-length Lactobacillus plantarum GAD and that the sequence is $100\%$ and $72\%$ identical to the regions of Lactobacillus plantarum GAD and Lactococcus lactis GAD sequences deposited in GenBank, accession nos: NP786643 and NP267446, respectively. The amino acid sequence deduced from the cloned Lactobacillus plantarum GAD gene showed $100\%$ and $68\%$ identities to the GAD sequences deduced from the genes of the NP786643 and NP267446, respectively. To express the GAD protein in E. coli, an expression vector with the GAD gene (pkk/GAD) was constructed and used to transform the UT481 E. coli strain and the expression was confirmed by analyzing the enzyme activity. The Lactobacillus plantarum GAD gene obtained may facilitate the study of the molecular mechanisms regulating GABA metabolism in lactic acid bacteria.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• 제35권4호
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• pp.258-265
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• 2024

Objectives: This study aimed to examine sex differences in the risk factors associated with generalized anxiety disorder (GAD) among Korean adolescents to provide insights for drafting more effective prevention strategies. Methods: Data from 51845 middle and high school students in the 18th Korea Youth Risk Behavior Web-based Survey were analyzed. GAD was assessed using the 7-item Generalized Anxiety Disorder tool, and factors such as grade, academic performance, economic status, living arrangements, smoking, drinking, sexual experience, and physical activity were included. The prevalence of GAD and its association with these factors were compared between male and female students using chi-square tests and logistic regression. Odds ratios were compared statistically to identify sex-specific differences. Results: GAD prevalence was higher among girls (42.1%) than boys (30.1%). Both sexes showed increased GAD risk with lower academic performance, lower economic status, smoking, drinking, and sexual experience. Boys living apart from their families had a higher GAD risk, but this was not significant for girls. Additionally, smoking and drinking were associated with a higher increase in GAD risk in girls than in boys. Conclusion: This study underscores the importance of considering sex differences in the prevention of GAD among adolescents. Tailored sex-specific interventions are crucial for effective prevention and management of GAD in Korean adolescents.

• 대한불안의학회지
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• 제13권2호
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• pp.100-107
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• 2017

Objective : The purpose of this study was to investigate strategies used to cope with stress and the cognitive characteristics of somatic symptom perception in patients with generalized anxiety disorder(GAD). Methods : A total of 55 patients meeting DSM-5 criteria for GAD and 55 normal controls were recruited for participation in this study. We evaluated subjects using The Way of Stress Coping Questionnaire (SCQ), Somato-Sensory Amplification Scale (SSAS), Symptom Interpretation Questionnaire (SIQ), and the Generalized Anxiety Disorder for 7 Item (GAD-7). We analyzed data using an independent t-test and Pearson's correlation analysis. Results : In terms of SCQ, GAD patients presented significantly lower scores on seeking social support and higher scores on wishful thinking than normal controls. GAD patients had significantly greater amplification of physical sensation in SSAS and higher scores in physical, psychological interpretation subsets of SIQ than normal controls. GAD-7 scores were positively correlated with physical interpretation scores on SIQ. Conclusion : Results reveal that patients with GAD have insufficient coping strategies for stress, greater amplification of body sensations, and tendency towards a physical, psychological interpretation of somatic symptoms.

• 대한소아치과학회지
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• 제48권3호
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• pp.269-279
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• 2021

이 연구의 목적은 코로나바이러스감염증-2019의 전파가 학령기 아동과 부모의 심리에 미친 영향을 수면장애와 우울증의 측면에서 평가하는 것이었다. 2020년 4월 2일부터 2020년 4월 25일까지 강동 경희대학교 치과병원 소아치과를 방문한 123명의 부모와 108명의 학령기 아동을 대상으로 직접 기입 방식의 설문조사를 실시하였다. 참가자들은 Pittsburgh Sleep Quality Index, Generalized Anxiety Disorder (GAD) - 7, Center for Epidemiology Scale for Depression 로 평가되었다. 로지스틱 회기 분석은 유의 수준 5%로 시행되었다. 부모의 GAD, 우울증, 낮은 수면의 질은 각각 34.1%, 17.1%, 44.7%에서 나타났다. 아동의 GAD 유병률은 20.4%였다. 로지스틱 회기분석 결과 코로나바이러스 관련 안전재난문자로 인한 스트레스가 부모의 불안장애, 우울증과 관련이 있었다. 아동의 경우, 코로나바이러스감염증 발생 이후의 감정 변화 정도가 GAD와 연관성이 있었다. 이 연구를 통해 코로나바이러스감염증-2019의 유행으로 인한 어린이와 보호자들의 심리적 상태 변화가 있음을 확인하였고, 치과진료 시 이들의 심리상태를 고려해야 하겠다.

• 한국심리학회지:학교
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• 제16권2호
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• pp.207-230
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• 2019

본 연구는 범불안 성향의 대학생을 대상으로 주의편향 및 범불안 증상에 대한 주의 피드백 인식 및 조절 훈련의 효과를 확인하였다. 범불안장애 척도(GAD-7) 10점 이상 또는 펜실베니아 걱정 질문지 척도(K-PSWQ) 56점 이상이며, 주의편향을 나타내는 범불안 성향의 대학생 31명을 선발하여 주의 피드백 인식 및 조절 훈련(Attention Feedback Awareness and Control Training, A-FACT) 집단(n = 11), 주의편향수정(Attention Bias Modification, ABM) 집단(n = 10) 및 활성 위약 통제(Active Placebo Control, APC) 집단(n = 10)에 무선할당하였다. A-FACT 집단은 탐침탐사과제를 이용한 주의편향수정 훈련 시, 개인의 기저반응시간(Baseline Neutral Response time, BNR)을 기준으로 편향 여부에 대한 피드백을 실시간으로 제공받았다. ABM 집단은 기존 ABM 절차와 동일하며, APC 집단은 주의편향을 감소시키기 위한 프로그램이라는 안내는 주어지지만 피드백이 주어지지 않는 탐침탐사과제를 실시하였다. 총 8회기로 구성되어, 주 2회로 4주간 진행되었다. 주의편향수정 훈련의 효과는 주의편향점수(Attention Bias Score, ABS)와 GAD-7, K-PSWQ 및 상태 및 특성 불안 검사(K-STAI) 점수 변화로 평가하였다. 반복측정 변량분석 결과, A-FACT 집단은 ABM 집단과 APC 집단에 비해 ABS가 유의하게 감소하였으며, GAD-7, K-PSWQ 및 K-STAI 점수도 유의하게 감소하였다. 이는 주의편향수정 훈련 시 편향에 대한 피드백을 통해 편향의 여부를 인식시키고 이를 통한 주의의 자기 조절적 통제가 주의편향 및 범불안장애 증상 경감에 효과적일 가능성을 시사한다.

• 한국미생물·생명공학회지
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• 제43권3호
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• pp.300-305
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• 2015

효율적인 γ-aminobutyric acid (GABA)의 생산을 위해 Lactobacillus plantarum WCFS1로부터 글루탐산 탈탄산효소(glutamate decarboxylase, GAD)를 대장균에 발현, 정제 후 silica beads에 covalent coupling 방법을 이용하여 고정화하였다. 고정화된 효소의 특성을 고정화하지 않은 효소와 비교한 결과, 모든 pH의 범위(pH 3.5–6.0)에서 80% 이상의 활성을 나타내었으며 pH 안정성과 열 안정성 모두 증대되었다. 이 고정화 효소를 packed-bed reactor에 충진하여 GABA의 생산성을 확인한 결과 1리터당 1시간에 최대 41.7 g의 GABA 생산이 가능한 것으로 확인되었다.

• 대한불안의학회지
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• 제18권2호
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• pp.101-108
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• 2022

Objective : The purpose of this study was to investigate depression, anxiety, trauma experience, and suicidal tendency among patients who were hospitalized in a community treatment center and to identify the factors that were associated with each symptom. Methods : A total of 213 patients who were hospitalized in the Tae-reung community treatment center between March 16, 2020 and April 30, 2020 were recruited into this study. A self-report questionnaire or interview by mental health professionals was used to assess PHQ-2, GAD-7, PC-PTSC and P4 screener. In 90 patients who completed the survey, multiple regression modeling was conducted to identify the burden of each psychiatric symptoms and associated demographic characteristics. Results : Female gender was significantly positively associated with GAD-7 score, score of each GAD-7 subscale of 1, 2, 3 or 4 (nervous, control of worry, worry, and trouble relaxing), and sum of GAD-7 subscale scores reflecting cognitive and emotional experience of anxiety (1. nervous+2. control of worry+3. worry+7. afraid). Previous psychiatric history and, number of counseling service used were significantly positively associated with the PHQ-2 score and the duration of isolation was significantly negatively associated with the PHQ-2 score. Conclusion : These results showed that depression and anxiety symptoms were more prevalent in COVID-19 patients at the community treatment center than in the general population. Among the demographic characteristics, female gender was positively associated with cognitive and emotional experience of anxiety. It shows the necessity of an appropriate psychiatric intervention protocol and health-policy preparation for community treatment centers.

• KSBB Journal
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• 제16권1호
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• pp.36-40
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• 2001

In order to study the molecular mechanism of $\gamma$-aminobutyric acid (GABA) production in plants, we cloned and sequenced a partial glutamate decarboxylase (GAD) cDNA from the Arabidopsis thaliana cDNA library, using primers targeted at highly conserved sequences of the petunia GAD gene. The cDNA fragment was inserted into TA cloning vector with T7 promoter and the recombinant plasmid obtained was used to transform E. coli. The plasmid DNA purified from the transformed E. coli was digested with EcoRI and the presence of the insert was confirmed. Nucleotide sequence analysis showed that the fragment is a partial Arabidopsis thaliana GAD gene and that the sequence showed 98% and 78% identity to the region of the putative Arabidopsis thaliana GAD sequences deposited in GenBank, Accession nos: U46665 and U10034, respectively. The amino acid sequence deduced from the partial Arabidopsis thaliana GAD gene showed 99% and 91% identities to the GAD sequences deduced from the genes of the U46665 and U10034, respectively. The partial cDNA sequence determined may facilitate the study of the molecular mechanism of GABA metabolism in plants.