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http://dx.doi.org/10.4014/mbl.1509.09003

Production of γ-Aminobutyric Acid Using Immobilized Glutamate Decarboxylase from Lactobacillus plantarum  

Lee, Sang-Jae (Major in Food Biotechnology, Silla University)
Lee, Han-Seung (Major in Food Biotechnology, Silla University)
Lee, Dong-Woo (School of Applied Biosciences, Kyungpook National University)
Publication Information
Microbiology and Biotechnology Letters / v.43, no.3, 2015 , pp. 300-305 More about this Journal
Abstract
The glutamate decarboxylase gene (gadB) from Lactobacillus plantarum WCFS1 was cloned and expressed as an N-terminal hexa-histidine-tagged fusion protein in Escherichia coli BL21 (DE3) as the host strain. Purified glutamate decarboxylase (GAD) was immobilized onto porous silica beads by covalent coupling. The pH dependence of activity and stability of the immobilized GAD was significantly altered, when compared to those of the free enzyme. Immobilized GAD was stable in the range of pH 3.5 to 6.0. The resulting packed-bed reactor produced 41.7 g of γ-aminobutyric acid/l·h at 45℃.
Keywords
Glutamate decarboxylase; immobilization; γ -aminobutyric acid; Lactobacillus plantarum; packed-bed reactor;
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