• Korean Journal of Microbiology
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• v.47 no.4
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• pp.316-322
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• 2011

Lactobacillus sakei OPK2-59 isolated from kimchi was found to have ${\gamma}$-aminobutyric acid (GABA) producing ability and glutamate decarboxylase (GAD) activity. When the Lactobacillus sakei OPK2-59 was cultured in MRS broth with 59.13 mM and 177.40 mM monosodium glutamate (MSG), the optimum temperature range and pH for growth were $25-37^{\circ}C$ and pH 6.5, respectively. GABA conversion rates in MRS broth with 59.13 mM and 177.40 mM MSG were 99.58% and 31.00%, respectively at $25^{\circ}C$ and 48 h of cultivation. By using the cell free extract of Lactobacillus sakei OPK2-59, MSG was converted to GABA and the conversion rate was 78.51% at $30^{\circ}C$, pH 5. Conversion of MSG to GABA was enhanced by adding salts such as $CaCl_2$, $FeCl_3$, $MgCl_2$. These data suggest that the ability of Lactobacillus sakei OPK2-59 to produce GABA results from the activity of GAD in the cells and GABA conversion by the cell extract containing GAD can be enhanced by $CaCl_2$, $FeCl_3$, $MgCl_2$.

• Korean Journal of School Psychology
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• v.16 no.2
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• pp.207-230
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• 2019

This study examined the effect of Attention Feedback Awareness and Control Training(A-FACT) on attention bias and generalized anxiety symptoms in college students. A total of 31 college students with at least 10 points on the Generalized Anxiety Disorder 7-item (GAD-7) scale or at least 56 points on the Korean version of the Penn State Worry Questionnaire (K-PSWQ) with attention bias were randomly assigned to one of three groups: A-FACT( n = 11), Attention Bias Modification (ABM)(n = 10) and Active Placebo Control (APC)(n = 10). Participants in A-FACT group received real-time feedback on attention bias based on their Baseline Neutral Response time(BNR) during A-FACT using a dot probe task. Participants in the ABM group received standard ABM, and those in the APC performed a dot probe task that they were informed was a program to reduce attention bias, but feedback was not provided. A total of eight sessions was conducted twice a week over a 4-week period. After every two sessions, GAD-7, K-PSWQ and K-STAI were rated. The effect of attention bias modification training was rated by changes in the Attention Bias Score(ABS), and in GAD-7, K-PSWQ and K-STAI scores. The results of repeated measure ANOVA indicated that the A-FACT group showed a significant decrease in ABS as well as in GAD-7, K-PSWQ and K-STAI scores compared to the other groups. Current results suggest that self-regulatory control of attention, that is, recognition of bias through feedback in A-FACT, may be effective in alleviating attention bias and generalized anxiety symptoms by recognizing bias through feedback on bias in attention bias modification training.

• Journal of Applied Biological Chemistry
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• v.43 no.2
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• pp.69-73
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• 2000

In order to understand the biological role of calmodulin in plants, transgenic plants expressing a mutant calmodulin (VU-4, Iys to ile-115) have been analyzed. We found that tobacco plants expressing VU-4 calmodulin have approximately twofold higher $\gamma$-aminobutyric acid (GABA) levels than the control plants. Cell suspension cultures established from the stem explants of the transgenic tobacco seedlings also have higher levels of GABA than the control cell cultures. Specific activity of glutamate decarboxylase (GAD), which catalyzes the decarboxylation of glutamate to $CO_2$ and GABA, of the transgenic tobacco cell extracts was about twofold higher than the activity of the control cell extracts. Western-blot analysis showed that the GAD is highly expressed in the transgenic tobacco plants. GAD partially purified from tobacco cell extracts showed approximately threefold $Ca^{2+}$/calmodulin-dependent activation. These data suggest that GABA synthesis in the transgenic tobacco plants is elevated, possibly due to higher levels of the calmodulin-dependent GAD enzyme and/or as a result of enhanced activation due to increased levels of the foreign calmodulin.

• Journal of Microbiology and Biotechnology
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• v.27 no.9
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• pp.1664-1669
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• 2017

Gamma-aminobutyric acid is a precursor of nylon-4, which is a promising heat-resistant biopolymer. GABA can be produced from the decarboxylation of glutamate by glutamate decarboxylase. In this study, a synthetic scaffold complex strategy was employed involving the Neurospora crassa glutamate decarboxylase (GadB) and Escherichia coli GABA antiporter (GadC) to improve GABA production. To construct the complex, the SH3 domain was attached to the N. crassa GadB, and the SH3 ligand was attached to the N-terminus, middle, and C-terminus of E. coli GadC. In the C-terminus model, 5.8 g/l of GABA concentration was obtained from 10 g/l glutamate. When a competing pathway engineered strain was used, the final GABA concentration was further increased to 5.94 g/l, which corresponds to 97.5% of GABA yield. With the introduction of the scaffold complex, the GABA productivity increased by 2.9 folds during the initial culture period.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.54 no.4
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• pp.543-551
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• 2021

Changes in gluten surface hydrophobicity, which play an important role in the functional characteristics of protein, were measured according to various protein concentrations, pH levels, electrolytes concentrations, and alginate molecular weights using 8-anilino-1-naphthalene sulfonic acid (ANS) as a fluorescent probe. Gluten surface hydrophobicity decreased as gluten concentration increased, reaching a maximum pH of 7.0. The effects of alginate molecular weights and alginate concentration on the surface hydrophobicity, emulsifying activity index (EAI), and emulsion stability index (ESI) of gluten-sodium alginate dispersion (GAD) were measured. Gluten surface hydrophobicity rapidly increased the asl NaCl concentration of gluten solution up to 300 mM and showed no significant increase above 300 mM. However, gluten surface hydrophobicity notably decreased until the concentration of CaCl₂ and MgCl₂ reached 30 mM, indicating no significant variations above 30 mM. GAD surface hydrophobicity increased as the concentration and molecular weight of sodium alginate increased, however, gluten concentration increased as the GAD surface hydrophobicity decreased. The EAI and ESI of GAD increased as both molecular weight and concentration of sodium alginate increased.

• Anxiety and mood
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• v.18 no.2
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• pp.101-108
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• 2022

Objective : The purpose of this study was to investigate depression, anxiety, trauma experience, and suicidal tendency among patients who were hospitalized in a community treatment center and to identify the factors that were associated with each symptom. Methods : A total of 213 patients who were hospitalized in the Tae-reung community treatment center between March 16, 2020 and April 30, 2020 were recruited into this study. A self-report questionnaire or interview by mental health professionals was used to assess PHQ-2, GAD-7, PC-PTSC and P4 screener. In 90 patients who completed the survey, multiple regression modeling was conducted to identify the burden of each psychiatric symptoms and associated demographic characteristics. Results : Female gender was significantly positively associated with GAD-7 score, score of each GAD-7 subscale of 1, 2, 3 or 4 (nervous, control of worry, worry, and trouble relaxing), and sum of GAD-7 subscale scores reflecting cognitive and emotional experience of anxiety (1. nervous+2. control of worry+3. worry+7. afraid). Previous psychiatric history and, number of counseling service used were significantly positively associated with the PHQ-2 score and the duration of isolation was significantly negatively associated with the PHQ-2 score. Conclusion : These results showed that depression and anxiety symptoms were more prevalent in COVID-19 patients at the community treatment center than in the general population. Among the demographic characteristics, female gender was positively associated with cognitive and emotional experience of anxiety. It shows the necessity of an appropriate psychiatric intervention protocol and health-policy preparation for community treatment centers.

• Journal of the Korean Society of Food Culture
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• v.39 no.3
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• pp.127-137
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• 2024

This study examined dietary habits and mental health according to generalized anxiety disorders (GAD) and caffeine consumption in adolescents. The data were collected from the 18^th Korea Youth Risk Behavior Survey with a sample of 51,850 students. The proportion of students in the GAD High-Risk·Caffeine consumption group (GHRCC) who consumed sweet drinks (p< .001), fast food (p< .001), and midnight meals (p< .001) every day was 15.6, 21.9, and 22.4%, respectively. These proportions were higher than those of students who consumed sweet drinks, fast food, and midnight meals 1-2 times a week, 3-4 times a week, or not at all. In addition, the proportions of GHRCC among students who responded "yes" to experiencing sadness and despair (p< .001), suicidal thoughts (p< .001), suicide plans (p< .001), suicide attempts (p< .001), and loneliness (p< .001) were higher than students who responded "no." The GAD High-Risk group (GHR) had higher odds with the consumption of midnight meals (OR: 2.51, 95% CI: 2.10-2.99), caffeine consumption (OR: 2.46, 95% CI: 2.21-2.74), and fast food (OR: 2.11, 95% CI: 1.75-2.55) than GAD Low-Risk group. Based on the above results, the risk of GHR was higher among students who consumed midnight meals, caffeine, and fast food daily than those who did not.

• Proceedings of the Korea Information Processing Society Conference
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• 2018.05a
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• pp.317-319
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• 2018

최근 발생하는 데이터는 대용량이며, 형태가 다양하고 빠르게 생성되는 특징이 있다. 이러한 데이터는 CPU, 인메모리 기반인 기존의 데이터 처리 시스템에서 처리하는데 많은 시간이 소모된다. 이 문제를 해결하기 위해 GPU 기반 데이터 집약 시스템이 출현하기 시작했다. 하지만, 이러한 시스템의 성능을 종합적으로 측정하는 테스트 결과는 시스템마다 다른 기준으로 제공하고 있다. 이에 따라, 개발자 및 사용자는 성능 병목 현상을 탐색하고 해결하는 데 큰 어려움을 겪을 수 있다. 즉, 이러한 다른 기준으로는 개발자 및 사용자가 시스템의 통합적인 성능 비교 분석을 수행하기 힘들다. 이러한 문제를 해결하기 위해서, 본 논문은 원스탑 테스팅 프레임워크인 BenchGAD 를 제안하고자한다.

• Journal of Aquaculture
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• v.3 no.1
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• pp.65-77
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• 1990

The growth of ark shell, Anadara broughtonii was compared between two areas, $Gad\v{o}gdo$ in Jinhae Bay and Namhae located in the southern coast of Korea from May 1986 to October 1987. The ark shells in Namhae grew from 1.38$\pm$0.32 em to 7.20$\pm$0.30 em in shell length, while those in $Gad\v{o}gdo$ grew from 1.38$\pm$0.32 em to 6,41$\pm$0.30 cm in 17 months. Shell height, shell breadth and total weigth of the ark shells in Namhae were also greater than those from $Gad\v{o}gdo$. Bottom quality of $Gad\v{o}gdo$ showed negative skewness, and that of Namhae was positive skewness. Negative skewness of $Gad\v{o}gdo$ seems to be caused by the effect of strong tidal current. This may indicate that Namhae is better area than $Gad\v{o}gdo$ for the culture of the ark shell.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.270-270
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• 1994

돼지 뇌조직으로부터 순수 분리 정제된 Glutamate decarboxylase (GAD)는 효소 dimer당 0.8mole 보조 인자인 pyridoxal-5-phosphate(PLP)가 강하게 binding되어 있었다. 이러한 부분적으로 resolved된 효소에 외부로부터 PLP를 넣어주면 효소의 활성도는 최대값으로 증가하였다. 정제된 GAD는 sulfydryl시약에 의한 화학변형에 의하여 효소의 활성도를 상실하였으며 환원제인 dithiothreitol이나 2-mercaptoethanol의 첨가에 의하여 효소의 활성도가 복구되는 것으로 보아 효소의 활성부위의 활성에 직접 관여하는 중요한 cysteinyl잔기가 존재하고 있는 것을 알 수 있다.