• Journal of Life Science
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• v.18 no.8
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• pp.1072-1082
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• 2008

The purpose of this study was to report the difference of nutritional factors for high school female tennis players pre and during the competition. A total of 7 tennis players (4 elite and 3 non-elite) participated in this study. All participants reported the total food lists and amounts of ingestion prior to 3 days and during 3 days the competition. The total calorie of prior to the competition for elite group (2869.7${\pm}$426.6 kcal) was significantly higher than non-elite group (1743.4${\pm}$220.8 kcal). The amount of carbohydrate for elite group (pre: 242.2${\pm}$188.4 g, during: 241.6${\pm}$ 201.4 g) was statistically higher than non-elite group (pre: 173.9${\pm}$127.1 g, during: 206.8${\pm}$123.7 g) pre and during the competition. There were no different amount of protein and fat between two group before and during the competition. The amount of calcium and phosphorus were also no different among the groups The consumption of iron was different prior to the competition (15.95${\pm}$3.09 vs. 10.36${\pm}$2.40 mg). The elite group (1.76${\pm}$0.30 mg) consumed vitamin B1 more than non-elite group (1.21${\pm}$0.23 mg) during the competition. No different other vitamines between two groups pre and during the competition.

• Journal of Embryo Transfer
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• v.30 no.3
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• pp.149-159
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• 2015

This study was designed to know the possibility in repeat uses of elite donor cows for getting mass production of OPU-derived embryo production (OPU-IVP). Ultrasound transvaginal ovum pick-up (OPU) performed in 6 Korean native cows was aged 4 to 10 years old. The aspiration of immature oocytes for OPU derived embryo was carried out 2 times per week, and OPU-IVP of $1^{st}$ period was carried out 22~48 sessions from each donors. And the break time for OPU-IVP of $2^{nd}$ period after $1^{st}$ OPU from each donors were 2~25 months. The OPU-IVP of $2^{nd}$ period each donors conducted total 15~65 times for 2~8 months by an ultrasonographic, was guided follicular aspiration system. The average numbers of collected oocytes, grade 1 + grade 2(G1+G2) oocytes and cleavage embryo from $1^{st}$ period OPU-IVP were significantly differences between donors (p<0.05). Total collected oocytes of donor D were significantly higher compared with donors of A, B, C, E and F (average 17.0 per session vs. 11.2, 10.1, 8.5, 10.2 and 9.6; p<0.05) and also oocytes of G1+G2 were significantly higher compared with r A and D and subsequently to donors of B, C, E and F (average 7.9 and 8.5 per session vs. 5.0, 2.7, 6.0 and 1.6; p<0.05). Cleavage rate of donor D was significantly higher compared with donors of A, B, C, E and F (average 13.1 per session vs. 10.1, 9.1, 6.9, 8.9 and 6.7; p<0.05). The average numbers of OPU-IVP for $1^{st}$ period was significantly higher from donors of B, D and E than those from donors of A, C and F (average 6.5, 7.1 and 6.5 per session vs. 3.5, 4.2 and 2.8; p<0.05). The possibility investigation of $2^{nd}$ OPU-IVP was carried out after 2~25 months rest periods from $1^{st}$ period OPU session. Total average numbers of collected oocytes, cleavages and blastocyst development rates were significantly higher from $1^{st}$ period OPU compared with $2^{nd}$ period one (p<0.05). The OPU-IVP efficiency by break for more embryo production from elite cow was analysis comparing without rest of donor A, under 6 months rest period as B and over 6 months rest period as C and then the average numbers of collected oocytes, cleavages and blastocysts were significantly higher from A group (11.8, 9.5 and 5.2 per session) than those from B and C groups (7.9, 6.2 and 2.6 vs. 9.2, 7.5 and 3.9, p<0.05), and also C group was significantly higher than B group. In conclusion, $1^{st}$ period OPU-IVP was more efficient compared with $2^{nd}$ period repeated uses of donor, and the break times for additional production of embryo on donor were needed more than over 6 months after $1^{st}$ period OPU-IVP. This repeating uses of elite donor cows given more emphasis for getting the opportunity on mass production of elite cow OPU-IVP embryo should be increased G1+G2 possibility of genetic improvement of livestock within short period.

• Plant Biotechnology Reports
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• v.2 no.4
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• pp.259-265
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• 2008

Picea koraiensis, called Korean spruce, is an evergreen tree and found mostly in northeast Asia. In this study, plant regeneration via somatic embryogenesis from open-pollinated immature zygotic embryos of nine geno-types of elite trees was established. Immature zygotic embryos were cultured onto RJW medium modified from 505 medium with $21.48{\mu}M$ NAA, $2.22{\mu}M$ BA, and $2.32{\mu}M$ KT. The average frequency for all nine genotypes was 74.2%. Embryogenic calluses of the nine genotypes of elite trees were subcultured on RJW basal medium containing $8.06{\mu}M$ NAA, $1.11{\mu}M$ BA, and $1.16{\mu}M$ kinetin. The calluses of three lines, $3^{\sharp}$, $9^{\sharp}$, and $2^{\sharp}$, were actively proliferated but others were not. Somatic embryogenesis was induced from the embryogenic callus in genotypes of $3^{\sharp}$, $9^{\sharp}$, and $2^{\sharp}$ on RJW medium with ABA and $60g\;l^{-1}$ sucrose. Cotyledonary somatic embryos were subjected to a drying process. The drying of embryos by uncapping the culture bottle for 5 days on a clean bench resulted in a high frequency of germination of somatic embryos (87% in RJW medium). However, plantlet conversion from germinated embryos was greatly reduced and the optimal medium for plant conversion was 1/2 WPM or 1/2 BMI medium. In conclusion, we have, for the first time, established a plant regeneration system via somatic embryogenesis in the Korean spruce, which can be applied for rapid micropropagation of elite trees.

• Korean Journal of Applied Biomechanics
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• v.16 no.2
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• pp.45-53
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• 2006

The purpose of this study was to analyze exercise related mechanical variables according to performance levels and Kumi-kata types in elite judo players (G1) and non-elite judo players, namely university players (G2). To achieve this purpose, three players in G1 whose main special skill was Morote-Seoinage and three university judo players(G2) was selected as comparative group. Then they were examined for distinguishing A and B types of Kumi-kata. Analyzed variables were the time required to show skills, knee degree, elbow degree. After analysing this study, conclusions were derived as follows. 1. In total necessary time of showing skills according to group of Kumi-kata type, G2 was longer than G1 in both A type (20.9%) and B type (23.7%). In necessary time of phase, in only 3P, G1 was shorter than G2 in A type (50%) and B type (75%). There was no difference in time required of 1P and 2P according to Kumi-kata type of group and in only 3P, B type was shorter than A type in both B type (75%) of G1 and B type (50%) of G2 2 There was no difference in elbow degree of offensive arm according to group of Kumi-kata type, however in A and B types, G1 could use skills by extending in kake phase, but G2 could use skills by bending. Elbow degree of offensive arm according to Kumi-kata type of group showed difference in E1. and F2 of G1. A and B types of G1 extended elbow degree in Kake phase, but G2 bent elbow degree so exercise program which could movable range extensively in Kake phase is needed.

• Exercise Science
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• v.21 no.2
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• pp.183-190
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• 2012

This study is to evaluate the availability of cardiorespiratory fitness measurement by 20 m shuttle run test based upon energy contribution rates of elite athletes in different sports type. Sixty-seven elite athletes attending K national university participated in this study. They were divided by three groups based upon sports type, composed of Anaerobic Group (sprint, jumps, weightlifting, throw; n=35), Aerobic Group (medium-long distance; n=9), and Combat Sport Group (judo; n=23). 20 m shuttle run test was conducted by Leger et al.(1982) method and calculating acceleration using measured shuttle run repetitions was conducted by Brewer et al.(1988) method. To test the usefulness of VO₂max, graded exercise treadmill test was conducted and standing long jump and 50 m run were measured as power fitness factors. Z-jump was used for measuring power, agility, and muscular endurance. Standing long jump and 50 m run of Anaerobic Group (AnG) was significantly higher than that of Aerobic Group (AeG) and Combat Sport Group (CG) (p<0.05). However, Z-jump of CG was significantly higher than that of AnG and AeG(p<.05). There was a higher correlation of 20 m shuttle run test and VO₂max in AnG(r= 0.577, p<.0001) and CG(r= 0.760, p<.0001). Otherwise, there was a low correlation of 20 m shuttle run test and VO₂max in AeG. There was no significant group difference to test the availability of 20 m shuttle run test and there was a reduced error when converting 20 m shuttle run results into VO₂max. This study examined the usefulness of 20 m shuttle run test by converting 20 m shuttle run repetition results into VO₂max calculation, which showed reduced error. Therefore, this study confirmed that it would be needed to convert 20 m shuttle run results into VO₂max for universal and practical use in the field without dividing sports type.

• Proceedings of the KIEE Conference
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• 2006.10c
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• pp.512-514
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• 2006

본 논문의 목표는 디지털 방송 수신과 아날로그 방송 수신 및 방송 컨텐츠를 녹화, 저장, 재생이 가능할 뿐만 아니라 각종 멀티미디어 컨텐츠를 즐길 수 있고, 양방향 데이터 방송이 가능하며, 저장된 콘텐트를 스트리밍으로 개인 멀티미디어 플레이어로 스트리밍 이 가능한 다기능 일체형 개인 녹화기를 개발하는 것이다. 고품질 디지털 방송과 기존의 아날로그 방송을 고품질의 디지털 컨텐츠로 변환하여 저장하고, 이를 원하는 시점에 언제든지 볼 수 있을 뿐만 아니라 인터넷 접속하여 어디 서든지 개인 형 멀티미디어 플래이어를 이용하여 스트리밍으로 저장된 컨텐츠를 즐길 수 있다. 이러한 기능을 수행하기 위하여 상당한 성능의 MPU가 필요로 하게 된다. 본 논문에서는 이러한 조건을 만족시키기 위하여 Zoran의 G9Ellite라는 SOC를 채택하였다. 개발된 시스템을 테스트하기 위하여 현재 각 방송사들의 방송을 수신하고 요구된 기능을 테스트 하였다.

• Korean Journal of Exercise Nutrition
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• v.25 no.4
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• pp.10-16
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• 2021

[Purpose] The purpose of this study was to (i) assess hydration levels in elite male football players during a national team training camp before and during qualifying matches, (ii) evaluate the effect of coaching strategies for hydration based on feedback from hydration monitoring, and (iii) assess possible relationships between hydration status and training load or wellness markers. [Methods] Thirty-one male players (age 27±4 yrs; height 185±6 cm; weight 82.9±6.7 kg; body fat 10.4±2.3%) representing a national team from the Union of European Football Associations (UEFA) participated. The players were studied during three different national team training camps related to the UEFA Nations League tournament. Urine specific gravity (U_SG) was measured to assess hydration status. During all camps, the players were actively coached on improving strategies for hydration and given individual feedback on their test results. The training load was measured using GPS technology, and wellness questionnaires were completed. [Results] U_SG decreased progressively and significantly (p<0.005) during camp 1 and hydration status improved over the three camps, with fewer dehydrated and more well-hydrated players identified during the last part of camp 3. Significantly (p<0.05) higher U_SG values were observed 2 days prior to a match (MD-2) than on match day (MD); consequently, 52% of the players were dehydrated on MD-2 and only 6% on MD. No correlations were observed between hydration status and training load or wellness markers. [Conclusion] Dehydration is a challenge in elite male football, but continuous monitoring of hydration status and coaching on hydration strategies can lead to major improvements and reduce the degree of dehydration.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.194-194
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• 2022

Recently, food-induced allergies have emerged as major global concerns. In the past ten years, it has doubled in western nations, and it has also increased in Asia and Africa. In many cases of food allergy, peanut allergy is prevalent, typically permanent, and frequently life-threatening. Therefore, we utilized gene editing techniques on the three major allergen genes in peanuts, Ara h 1, Ara h 2, and Ara h 3. Using gibson assembly and golden gate assembly, we created two vectors, the gRNA-tRNA array CRISPR-Cas9 system and Prime-editing. Using LBA4404 strain and agrobacterium-mediated transformation, the vectors were transferred to two elite Korean peanut lines. After co-cultivation and tissue culture, we extracted the tissue cultured peanut DNA amplified the hygromycin resistance gene and Cas9 gene in the T-DNA region. The integration of the T-DNA region into the host genome was demonstrated by the presence of a specific band in some samples. There have only been a few reported peanut gene editing studies. So, this study will contribute to peanut allergy and gene editing research.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.200-200
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• 2022

Recently, food-induced allergies have emerged as major global concerns. In the past ten years, it has doubled in western nations, and it has also increased in Asia and Africa. In many cases of food allergy, peanut allergy is prevalent, typically permanent, and frequently life-threatening. Therefore, we utilized gene editing techniques on the three major allergen genes in peanuts, Ara h 1, Ara h 2, and Ara h 3. Using gibson assembly and golden gate assembly, we created two vectors, the gRNA-tRNA array CRISPR-Cas9 system and Prime-editing. Using LBA4404 strain and agrobacterium-mediated transformation, the vectors were transferred to two elite Korean peanut lines. After co-cultivation and tissue culture, we extracted the tissue cultured peanut DNA amplified the hygromycin resistance gene and Cas9 gene in the T-DNA region. The integration of the T-DNA region into the host genome was demonstrated by the presence of a specific band in some samples. There have only been a few reported peanut gene editing studies. So, this study will contribute to peanut allergy and gene editing research.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.37 no.9
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• pp.1077-1082
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• 2013

To improve cycling performance, the power output of the rider and the sources of resistance (e.g., air resistance, frame stiffness, and cycle mass) must be considered. From a mechanical viewpoint, we consider how the bike frame performance can be increased while decreasing the resistance. First, to improve the competitive ability of a racing track cycle, we should consider the stiffness of the bike frame including the pedal loading and aerodynamic effects when riding. Therefore, we obtained the cross-sectional area of each part of the bike frame and then aimed to minimize the drag force through an aerodynamic parametric study. In addition, the frame should remain safe under the loading applied by the rider. Under the guidelines of the UCI (union Cycliste Internationale) regulations, the bike frame has been evaluated under the proposed loading condition, and we developed a racing track cycle for elite cyclists.