• Journal of Applied Biological Chemistry
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• 제49권4호
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• pp.131-134
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• 2006

The in vitro anti-fungal activity of hydroxylated fatty acids obtained from microbial conversion by Psuedomonas aeruginosa PR3 using ricinoleic acid(RA), eicosadienoic acid(EDA) and conjugated linoleic acid(CLA) as substrates, was investigated. Bioconverted hydroxylated fatty acids showed different anti-fungal activities potentials against the range of phytopathogenic fungi such as Botrytis cinerea, Rhizoctonia solani, Fusarium oxysporum, Sclerotonia sclerotiorum, Colletotricum capsici, Fusarium solani and Phytophthora capsici. RA and EDA showed up to 50% fungal mycelial inhibition at the concentration of $5{\mu}l\;ml^{-1}$. RA, EDA and CLA also exhibited anti-fungal activities with minimum inhibitory concentration(MIC), ranging from 500 to $1000{\mu}g\;ml^{-1}$. Screening was also carried out using varied concentrations of bioconverted RA and EDA for determining the anti-fungal effect on the spore germination of different fungi. Bioconverted RA and EDA showed a considerable degree of spore germination inhibition.

• 대한설비공학회:학술대회논문집
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• 대한설비공학회 2008년도 동계학술발표대회 논문집
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• pp.330-333
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• 2008

The hybrid air-conditioner, where air is cooled both by convection and radiation, is developed. The indoor unit of the air-conditioner consists of radiation panel and dehumidification coil, where refrigeration R-134a is supplied by independent refrigeration cycles. Optimum refrigerant charge was 750g for both cycles. Optimum evaporation pressure was 3.7 bar for the radiation panel cycle and 3.9 bar for the dehumidification cycle. The cooling capacity of the radiation panel was 1.01 kW and that of the dehumidification coil was 0.94kW, which yielded COP of 3.3.

• 한국컴퓨터정보학회:학술대회논문집
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• 한국컴퓨터정보학회 2012년도 제46차 하계학술발표논문집 20권2호
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• pp.131-134
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• 2012

이 연구에서는 건강가정지원센터의 프로그램 활성화를 위해 가족의 가치관, 의사소통, 가족관계성 수준에 따라 개설하고자 하는 프로그램 필요도가 다르게 나타날 것이다라는 연구문제를 규명하기 위한 목적에서 출발하였다. 이러한 연구목적을 달성하기 위해 G광역시 서구지역에 거주하는 주민 299명을 대상으로 자기기입식 설문지 방식을 사용하였다. 분석결과, 가족의 가치관, 의사소통, 가족관계성 수준에서 평균미만집단이 평균이상집단과 비교하여 건강가족지원프로그램에 대한 필요도가 유의미하게 높게 나타났다. 따라서 건강가정지원센터의 사업 프로그램은 지역주민의 가족의 가치관, 의사소통, 가족관계성 수준을 반영한 필요도 중심의 활성화 방안을 모색해야 할 것이다.

• 한국펄프종이공학회:학술대회논문집
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• 한국펄프종이공학회 2008년도 춘계학술대회
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• pp.129-134
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• 2008

Properties of newly developed paper from Red Algae Pulp (RAP) were examined. The paper samples were prepared by mixing RAP fiber with wood fibers, HwBKP and SwBKP, to form a paper with $60g/m^2$ in weight. It was prepared in three to four different levels of refining degree and pressure so that it can reveal different bulk level in order to clearly compare the opacity at equivalent bulk for each furnish compositions. Printability of RAP fiber revealed superior effect on print through repression and initial ink absorption. Those properties are expected to improve further if printability improvement effect due to smoothness improvement is added.

• Journal of Radiation Protection and Research
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• 제45권2호
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• pp.76-80
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• 2020

Background: To improve the measurement accuracy of liquid-scintillation counting for activity standardization, it is necessary to significantly reduce the background caused by thermal noise or after-pulses. We have therefore improved a movable 3 photomultiplier (3PM)-γ coincidence-counting method using the logical sum of three double coincidences for β events. Materials and Methods: We designed a new data-acquisition system in which β events are obtained by counting the logical sum of three double coincidences. The change in β-detection efficiency can be derived by moving three photomultiplier tubes sequentially from the liquid-scintillation vial. The validity of the method was investigated by activity measurement of ¹³⁴Cs calibrated at the Korea Research Institute of Standards and Science (KRISS) with 4π(PC)β-γ(NaI(Tl)) coincidence counting using a proportional counter (PC) for the β detector. Results and Discussion: Measurements were taken over 14 counting intervals for each liquidscintillation sample by displacing three photomultiplier tubes up to 45 mm from the sample. The dead time in each β- and γ-counting channel was adjusted to be a non-extending type of 20 ㎲. The background ranged about 1.2-3.3 s^-1, such that the contributions of thermal noise or after-pulses were negligible. As the β-detection unit was moved away from the sample, the β-detection efficiencies varied between 0.54 and 0.81. The result obtained by the method at the reference date was 396.3 ± 1.7 kBq/g. This is consistent with the KRISS-certified value of 396.0 ± 2.0 kBq/g within the uncertainty range. Conclusion: The movable 3PM-γ method developed in the present work not only succeeded in reducing background counts to negligible levels but enabled β-detection efficiency to be varied by a geometrical method to apply the efficiency extrapolation method. Compared with our earlier work shown in the study of Hwang et al. [2], the measurement accuracy has much improved. Consequently, the method developed in this study is an improved method suitable for activity standardization of β-γ emitters.

• Asian Pacific Journal of Cancer Prevention
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• 제16권11호
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• pp.4727-4732
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• 2015

Background: Folylpolyglutamate synthetase (FPGS), an important enzyme in the folate metabolic pathway, plays a central role in intracellular accumulation of folate and antifolate in several mammalian cell types. Loss of FPGS activity results in decreased cellular levels of antifolates and consequently to polyglutamatable antifolates in acute lymphoblastic leukemia (ALL). Materials and Methods: During May 1997 and December 2003, 134 children diagnosed with ALL were recruited from one hospital in Thailand. We performed a mutation analysis in the coding regions of the FPGS gene and the association between single nucleotide polymorphisms (SNPs) within FPGS in a case-control sample of childhood ALL patients. Mutation screening was conducted by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and subsequently with direct sequencing (n=72). Association analysis between common FPGS variants and ALL risk was done in 98 childhood ALL cases and 95 healthy volunteers recruited as controls. Results: Seven SNPs in the FPGS coding region were identified by mutation analysis, 3 of which (IVS13+55C>T, g.1297T>G, and g.1508C>T) were recognized as novel SNPs. Association analysis revealed 3 of 6 SNPs to confer significant increase in ALL risk these being rs7039798 (p=0.014, OR=2.14), rs1544105 (p=0.010, OR= 2.24), and rs10106 (p=0.026, OR=1.99). Conclusions: These findings suggested that common genetic polymorphisms in the FPGS coding region including rs7039789, rs1544105, and rs10106 are significantly associated with increased ALL risk in Thai children.

• Asian-Australasian Journal of Animal Sciences
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• 제17권4호
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• pp.467-472
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• 2004

Eight primiparous Holstein cows, in late lactation (255$\pm$10 days in milk) and yielding 10.3$\pm$1.3 kg/d of 4% fat corrected milk (FCM) were allocated into two groups randomly. Two diets containing 30% wheat straw either untreated (UWS) or treated with Pleurotus ostreatus (FTWS) were offered as total mixed ration (TMR). In vivo digestibility of the diets was determined, using acid insoluble ash as a marker. Daily milk production was recorded and milk samples were collected and analysed. Diet FTWS resulted in significantly (p<0.05) higher dry matter intake (DMI) (12.2$\pm$0.86 vs. 10.6$\pm$1.3), DM digestibility (58.8 vs. 52.3) and milk yield (9 vs. 7.5 kg). Milk fat contents were 34.2 and 35.6 g/liter that did not differ between cows fed treated or untreated straw. However, the concentrations of lactose, solid non fat, total solids and milk protein for diets UWS and FTWS were 57.3 and 54.9, 98.9 and 93.2, 134.5 and 127.4, 35.7 and 32.3 g/l, respectively, which differed significantly (p<0.05). The average body weights gain (BWG) for UWS and FTWS were 272 and 743 g/d, respectively (p<0.05). The FCM yield per kg of DMI was similar (0.68 and 0.67 liter) for the two groups, but BWG/kgDMI was higher in the FTWS diet.

• 한국식품영양과학회지
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• 제32권1호
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• pp.35-41
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• 2003

본 연구는 식품에 존재하는 Y. enterocolitica균의 신속한 검출방법을 조사하기 위하여 이균에 특이적인 특이적인 ail, yst 및 virF 유전자와 Yersinia 속균을 구별하는 subgenus-specific Y16S primer를 도입하여 multiplex PCR을 수행하였으며 Y. enterocolitica균의 검출 민감도와 특이도 및 먹는 샘물과 야채류에서의 적용실험을 각각 조사하였다. PCR 특이성 실험에서는 Y enterorolitica ATCC 27729균은 355 bp(ail), 134 bp(yst) 및 200 bp(Y16S) 3종의 유전자에 대한 DNA 증폭밴드를 나타내었으며, Y. enteroculitica ATCC 9610 및 ATCC 23715는 yst와 Y16S 2종에만 증폭을 보였다 반면에 기타 비병원성 Yersinia균인 Y. frederikseni, Y. intemedia, Y kri-steneni, Y pseudotuberculosis 등은 Y16S에만 DNA밴드를 나타내었으나 기타 세균인 E. colt ATCC 25392, Shi. dysen-teri, S. aureus ATCC 25923, L. monocytognes ATCC 19111 균에서는 어떤 primer에서도 특이 DNA 밴드를 확인할 수 없었다 PCR 민감도는 다른 유전자에 비하여 yst 유전자가 Y. enterocolitica균에 가장 높은 민감도를 나타내었고, Y16S 유전자는 속과 종에 관계없이 높은 민감도를 나타내었다. 따가서, 먹샘물이나 야채류에 대한 병원성 Yersinia균의 지표유전자자로서 속을 대표하는 Y16S유전자와 종을 대표하는 yst유전자를 선정하였다. 수질 중 Y. enferocolitica의 검출한계를 알아보기 위하여 일반세균수가 3600 CFU/mL정도 함유된 먹는 샘물의 경우, DNA를 분리하지 않고 단순 열처리한 배양액을 DNA주형으로 사용하여 multiplex-PCR을 실시한 결과, Y16S 와 yst 유전자 모두 7$\times$$10^1$ CFU/mL 수준가지 검출할 수 있었으며, 일반세균수가 2.5$\times$$10^{5}$CFU/g 정도 함유된 상치는 7 및 7$\times$$10^1$CFU/g, 일반세균이 7.1$\times$$10^4$CFU/g정도 함유된 양송이버섯은 7 및 7$\times$$10^1$CFU/g 수준까지 검출할 수 있었다. 본 연구에서 나타난 바와 같이, 수질이나 야채류에서 Y16S와 yst 유전자를 혼합하여 Y. enterocolitica를 검출할 경우, DNA를 분리하지 않고 직접 whole cell을 lysate하여 DNA주형으로 사용하여도 2차 PCR을 수행할 경우에는 증균과정을 하지 않고도 민감도를 증진시키면서 신속하게 효율적으로 원하는 대상균을 검출할 수 있는 것으로 나타났다.다.

• International Journal of Oral Biology
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• 제46권3호
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• pp.134-139
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• 2021

Under physiological conditions, calcium (Ca²⁺) regulates essential functions of polarized secretory cells by the stimulation of specific Ca²⁺ signaling mechanisms, such as increases in intracellular Ca²⁺ concentration ([Ca²⁺]_i) via the store-operated Ca²⁺ entry (SOCE) and the receptor-operated Ca²⁺ entry (ROCE). Homer proteins are scaffold proteins that interact with G protein-coupled receptors, inositol 1,4,5-triphosphate (IP₃) receptors, Orai1-stromal interaction molecule 1, and transient receptor potential canonical (TRPC) channels. However, their role in the Ca²⁺ signaling in exocrine cells remains unknown. In this study, we investigated the role of Homer2 in the Ca²⁺ signaling and regulatory channels to mediate SOCE and ROCE in pancreatic acinar cells. Deletion of Homer2 (Homer2^-/-) markedly increased the expression of TRPC3, TRPC6, and Orai1 in pancreatic acinar cells, whereas these expressions showed no difference in whole brains of wild-type and Homer2^-/- mice. Furthermore, the response of Ca²⁺ entry by carbachol also showed significant changes to the patterns regulated by specific blockers of SOCE and ROCE in pancreatic acinar cells of Homer2^-/- mice. Thus, these results suggest that Homer2 plays a critical role in the regulatory action of the [Ca²⁺]_i via SOCE and ROCE in mouse pancreatic acinar cells.

• 한국식품저장유통학회지
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• 제16권1호
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• pp.134-137
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• 2009

자몽종자 추출물(GSE)을 0.02, 0.05, 0.08, 0.1%의 농도로 첨가하여 제조한 gelatin 필름의 물성과 항균효과에 대해 조사하였다. 필름의 인장강도는 0.1% GSE를 첨가하였을 경우 10.28 MPa로 대조구의 8.68 MPa에 비하여 1.60 MPa 증가하였으나, GSE 첨가량의 증가에 따라 유의적으로 증가하지는 않았다. 투습계수는 GSE의 첨가량이 증가할수록 감소되는 것을 확인할 수 있었는데, 대조구의 2.48 ngm/m2sPa 에 비하여 0.1% GSE의 첨가 시 2.18 ng $m/m^{2}sPa$이었다. GSE 첨가한 gelatin 필름의 항균활성의 경우, 0.1% GSE를 첨가 했을 때 E. coli의 경우 2.67 log CFU/g, L. monocytogenes 경우 3.15 log CFU/g 감소시키는 결과를 얻었다. 따라서 본 연구 결과, GSE를 첨가한 gelatin 필름이 가공식품의 포장재로써 식품에서 일부 위해미생물을 억제 할 가능성이 있다고 판단된다.