• Research in Vestibular Science
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• 제17권4호
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• pp.134-141
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• 2018

Objectives: We aimed to study the role of vestibular input on spatial memory performance in mice that had undergone bilateral surgical labyrinthectomy, semicircular canal (SCC) occlusion and 4G hypergravity exposure. Methods: Twelve to 16 weeks old ICR mice (n=30) were used for the experiment. The experimental group divided into 3 groups. One group had undergone bilateral chemical labyrinthectomy, and the other group had performed SCC occlusion surgery, and the last group was exposed to 4G hypergravity for 2 weeks. The movement of mice was recorded using camera in Y maze which had 3 radial arms (35 cm long, 7 cm high, 10 cm wide). We counted the number of visiting arms and analyzed the information of arm selection using program we developed before and after procedure. Results: The bilateral labyrinthectomy group which semicircular canal and otolithic function was impaired showed low behavioral performance and spacial memory. The semicircular canal occlusion with $CO_2$ laser group which only semicircular canal function was impaired showed no difference in performance activity and spatial memory. However the hypergravity exposure group in which only otolithic function impaired showed spatial memory function was affected but the behavioral performance was spared. The impairment of spatial memory recovered after a few days after exposure in hypergravity group. Conclusions: This spatial memory function was affected by bilateral vestibular loss. Space-related information processing seems to be determined by otolithic organ information rather than semicircular canals. Due to otolithic function impairment, spatial learning was impaired after exposure to gravity changes in animals and this impaired performance was compensated after normal gravity exposure.

• 한국가축번식학회지
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• 제21권2호
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• pp.167-176
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• 1997

본 연구는 수정란을 수란우에 이식하기 이전에 형질전환가능 수정란을 선발할 수 있다면 형질전환동물의 생산에 크게 도움이 되므로 3.2 kb $\beta$-actin promoter (lacZ/n대) DNA를 미세주입하여 배반포기배에서의 발현을 확인하여 이들을 선발할 수 있는가를 규명하고자 하엿다. 채란된 난포란은 10%FBS, 5$\mu\textrm{g}$/ml LH, 0.5$\mu\textrm{g}$/ml FSH, 100 unit/ml penicillin 및 100 $\mu\textrm{g}$/ml streptomycin이 함유된 TCM199에 22~24 시간동안 체외성숙을 유도후 5$\mu\textrm{g}$/ml heparin으로 수정능획득을 유도한 1$\times$106 sperm/ml의 정자로 체외수정을 시켰다. 체외수정후 18~20시간째에 vortexing에 의해 과립막세포를 제거하고 원심분리시켜 자/웅전핵이 확인되는 수정란의 핵에 3~4 ng/${\mu}\ell$ lacZ/neo DNA를 미세주입하였다. 모든 수정란의 배양은 3 mg/ml BSA, 20${\mu}\ell$/ml NEM AMINO acids 및 40${\mu}\ell$/ml BME amino acids가 함유되어 있는 CR1aa 배양액에 neo/DNA로 transfected 된 BRL 단층에서 실시하였다. G418에 대한 적정농도를 찾기 위하여 정상적인 수정란에 0, 50, 100 및 200 $\mu\textrm{g}$/ml G418를 첨가하여 배양한 결과 8일째에 30.3%(44/145), 8.7%(13/150), 0.7%(1/151) 및 0% (0/134)의 수정란이 배반포기까지 발달하였다. 그래서 본 실험에서는 일정하게 100$\mu\textrm{g}$/ml G418을 첨가하여 배양하였다. 총 1,127개의 수정란을 미세주입후 G418 없는 배양액에서 710개 (63.0%)가 분할하였다. 미세주입후 48시간째에 2-세포기이상 분할된 수정란을 대조구 및 100$\mu\textrm{g}$/ml G418처리구를 무작위로 할당하여 배양하였으며, 또한 740개의 정상수정란도 같은 반복수로 배양을 실시하였다. 미세주입한 수정란은 8일 후 11.6%(26/255) 및 5.2% (14/267)가 대조구 및 G418 처리구에서 배반포기까지 발달하였으며 정상수정란은 27.2% (151/740)가 배반포기 배까지 발달하였다. 미세주입후 대조구에서는 23.1$\pm$2.6/70.7$\pm$4.7 (32.7%)의 할구가 $\beta$-Gal 활력을 보였고, 반면에 100$\mu\textrm{g}$/ml G418 처리구에서는 40.3$\pm$4.1/48.8$\pm$7.5 (82.6%)가 $\beta$-Gal 활력을 보였다. 비록 mosaic 형태로 외래유전자가 발현되었지만 대조구에서 87.0% (26/30개) 배반포기가 $\beta$-Gal 활력을 보인 반면, G418 처리구에서는 모든 배반포기가 $\beta$-Gal 활력을 보였다 (P<0.05). 그러나 대조구 및 G418 처리구의 ICM colony에서는 영양배엽과 내배엽을 제외한 epiblast에서는 확인되지 않았다. 그러나 이 결과로부터 $\beta$-actin promoter/lacZ gene이 integration되지 않는 것인지 또는 다만 염색 확인이 되지 않는 것인지를 판단할 수는 없다. 이상의 결과는 미세주입후 G418에서 배양한 배반포기배에서는 대부분의 할구에서 주입된 gene을 발현하고 있었으나 ICM colony에서는 특히 epiblast에서는 발현되지 않거나 침묵하고 있었다. 비록 G418 처리구에서 훨씬 더 높은 비율로 주입된 gene이 발현되고 있으나 총세포수는 유의적으로 감소하여 이후 형질전환동물의 생산과 ES like-cell의 설립에는 감소될 것으로 사려된다. 그러나 형질전환 수정란의 선발 및 형질전환동물의 생산능력에 관해서는 더 많은 연구가 필요하다고 사려된다.

• 한국어병학회지
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• 제24권1호
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• pp.29-37
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• 2011

Nalidixic acid(NA), piromidic acid(PA)를 사육수온 ($13{\pm}1.5^{\circ}C$, $23{\pm}1.5^{\circ}C$)에 따라서 조피볼락(평균체중 $500{\pm}30\;g$)에 60 mg/kg의 농도로 1회 경구투여한 다음, 시간경과에 따라 혈청 내 잔류농도를 분석하였다. $23{\pm}1.5^{\circ}C$의 경우, 투여 후 NA는 24시간째($5.87\;{\mu}g/ml$), PA도 24시간째($0.43\;{\mu}g/ml$)에 각각 최대혈중농도에 도달하였다. $13{\pm}1.5^{\circ}C$의 경우, 투여 후 NA는 10시간 째($6.22\;{\mu}g/ml$), PA도 10시간째($1.57\;{\mu}g/ml$)에 각각 최대혈중농도에 도달하였다. PA의 조피볼락 혈중 내 흡수정도는 $23{\pm}1.5^{\circ}C$보다 $13{\pm}1.5^{\circ}C$에서 매우 높게 나타났으며, NA는 수온에 따른 차이가 크지 않았다. NA 및 PA의 조피볼락 혈중 내 소실정도는 $13{\pm}1.5^{\circ}C$ 보다 $23{\pm}1.5^{\circ}C$에서 두드러지게 빨랐다. NA 및 PA는 one-compartment model로 해석(WinNonlin program)하여 약물동태학적 변수(parameter)를 조사하였다. $23{\pm}1.5^{\circ}C$의 경우, 혈장농도-시간곡선하 면적(AUC)은 NA, PA가 각각 161.25 및 $41.57\;{\mu}g{\cdot}h/ml$, 반감기($T_{1/2}$)는 0.15 h 및 0.58 h, 혈중최고농도의 도달 시간($T_{max}$)은 12.29 및 8.24 h, 혈중최고농도($C_{max}$)는 3.85 및 $0.21\;{\mu}g/ml$로 계산되었다. $13{\pm}1.5^{\circ}C$의 경우, AUC는 NA, PA가 각각 134.42 및 $40.36\;{\mu}g{\cdot}h/ml$, $T_{1/2}$은 0.18 h 및 0.59 h, $T_{max}$는 10.67 및 21.15 h, $C_{max}$는 8.91 및 $3.09\;{\mu}g/ml$로 계산되었다.

• 한국버섯학회지
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• 제5권2호
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• pp.86-90
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• 2007

느타리버섯의 품종 다양화를 위해 단핵균주교잡에 의해 육성된 적단느타리버섯의 주요특성은 다음과 같다. 가. 균사생장적온은 $26{\sim}30^{\circ}C$이고 버섯발생 및 생육온도는 $18{\sim}23^{\circ}C$이었다. 나. 갓의 형태는 깊은깔대기형이며 다발형이고, 대는 가늘고 짧은형이며 갓, 대 모두 분홍색을 나타내었다. 다. 병재배시 배양일수는 $20^{\circ}C$에서 20일, 초발이소요일수는 $18{\sim}20^{\circ}C$재배조건에서 4일이며 생육일수는 4일이 소요되었다. 봉지재배에서는 균배양일수는 22일, 초발이 소요일수는 6일, 생육일수는 5일정도 소요되었다. 라. 버섯의 균일성에 있어서 갓색과 갓형태의 이형개체 발생은 없었고, 균사생장량 및 종균배양기간에서도 지역간 균일한 결과를 나타내었다. 마. 농가실증시험 결과 유효개체수는 850cc병재배시는 31.5개이며, 2kg 봉지재배시 47개체수를 얻었으며 수량은 병재배시 134.5g, 봉지재배시 350.0g의 수량을 얻었다.

• Natural Product Sciences
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• 제3권1호
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• pp.29-37
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• 1997

Protein Kinase C (PKC) is generally believed to play a central role in signal transduction, cellular growth control, gene expression, and tumor promotion. And it has been suggested that inhibitors of PKC might play important roles for the prevention and treatment of cancer. In order to investigate the possible inhibitors of PKC from natural products, PKC receptor binding assay was performed using bovine brain particulate as a source of PKC and the amount of $[^3H]Phorbol$ 12,13-dibutyrate (PDBu) bound to PKC was measured in the presence of test materials. Total methanol extracts from 100 kinds of natural products were partitioned into 3 fractions (n-hexane, ethyl acetate and aqueous layer) and their binding ability to the regulatory domain of PKC was evaluated. The ethyl acetate fractions of Morus alba $(roots,\;IC_{50}:\;156.6\;{\mu}g/ml)$, Rehmannia glutinosa $(roots,\;IC_{50}:\;134.3\;{\mu}g/ml)$, Lysimachia foenum-graecum $(roots,\;IC_{50}:\;167.8\;{\mu}g/ml)$, Polygonum cuspidata $(roots,\;IC_{50}:\;157.3\;{\mu}g/ml)$, Cnidium officinale $(aerial\;parts,\;IC_{50}:\;145.2\;{\mu}g/ml)$, and the hexane $(IC_{50}:\;179.3\;{\mu}g/ml)$ and the EtOAc fraction of Symplocarpus nipponicus $(roots,\;IC_{50}:\;155.9\;{\mu}g/ml)$ showed inhibitory activity of $[^3H]PDBu$ binding to PKC.

• Tuberculosis and Respiratory Diseases
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• 제82권2호
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• pp.143-150
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• 2019

Background: The purpose of this study was to analyze the relationship between the gene mutation patterns by the GenoType MTBDRplus (MTBDRplus) assay and the phenotypic drug susceptibility test (pDST) results of isoniazid (INH) and prothionamide (Pto). Methods: A total of 206 patients whose MTBDRplus assay results revealed katG or inhA mutations were enrolled in the study. The pDST results were compared to mutation patterns on the MTBDRplus assay. Results: The katG and inhA mutations were identified in 68.0% and 35.0% of patients, respectively. Among the 134 isolated katG mutations, three (2.2%), 127 (94.8%) and 11 (8.2%) were phenotypically resistant to low-level INH, high-level INH, and Pto, respectively. Among the 66 isolated inhA mutations, 34 (51.5%), 18 (27.3%) and 21 (31.8%) were phenotypically resistant to low-level INH, high-level INH, and Pto, respectively. Of the 34 phenotypic Pto resistant isolates, 21 (61.8%), 11 (32.4%), and two (5.9%) had inhA, katG, and both gene mutations. Conclusion: It is noted that Pto may still be selected as one of the appropriate multidrug-resistant tuberculosis regimen, although inhA mutation is detected by the MTBDRplus assay until pDST confirms a Pto resistance. The reporting of detailed mutation patterns of the MTBDRplus assay may be important for clinical practice, rather than simply presenting resistance or susceptibility test results.

• International Journal of Oral Biology
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• 제36권3호
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• pp.129-134
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• 2011

Eugenol is an essential oil found in cloves and cinnamon that is used widely in perfumes. However, the significant anesthetic and sedative effects of this compound have led to its use also in dental procedures. Recently, it was reported that eugenol induces apoptosis in several cancer cell types but the mechanism underlying this effect has remained unknown. In our current study, we examined whether the cytotoxic effects of eugenol upon human melanoma G361 cells are associated with cell cycle arrest and apoptosis using a range of methods including an XTT assay, Hoechst staining, immunocyto-chemistry, western blotting and flow cytometry. Eugenol treatment was found to decrease the viability of the G361 cells in both a time- and dose-dependent manner. The induction of apoptosis in eugenol-treated G361 cells was confirmed by the appearance of nuclear condensation, the release of both cytochrome c and AIF into the cytosol, the cleavage of PARP and DFF45, and the downregulation of procaspase-3 and -9. With regard to cell cycle arrest, a time-dependent decrease in cyclin A, cyclin D3, cyclin E, cdk2, cdk4, and cdc2 expression was observed in the cells after eugenol treatment. Flow cytometry using a FACScan further demonstrated that eugenol induces a cell cycle arrest at S phase. Our results thus suggest that the inhibition of G361 cell proliferation by eugenol is the result of an apoptotic response and an S phase arrest that is linked to the decreased expression of key cell cycle-related molecules.

• 약학회지
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• 제48권6호
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• pp.309-316
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• 2004

The present investigation was undertaken to find out the anticancer activity of monoterpene compounds. Monoterpenes showed generally the inhibitory effect on the proliferation o f L1210 cancer cells (cytotoxicity). Geraniol was found to exibit the most potent cytotoxic effect on L1210 cells with an IC50 values of $0.67{\mu}g/ml$. On the other hand, geraniol proved to be capable of stimulating the macrophage proliferation (135% of control). When the life prolonging activity of geraniol by daily oral administration of 0.1~10${\mu}g/10{\mu}l/20$ g body weight to Sarcoma 180 bearing ICR mouse was examined, there was also a significant elevation of survival (best result of 134% of control). The contradictory effects of geraniol on the proliferation of L1210 cells and macrophages proved to be accompanied by the coincident alterations of RNS (reactive nitrogen species) related enzymes activities such as inducible nitric oxide synthase (Inos) in macrophages and ROS (reactive oxygen species) related enzymes activities such as superoxide dismutase (SOD) in L1210 cells, respectively.

• 한국환경과학회지
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• 제9권2호
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• pp.145-149
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• 2000

The purpose of this study was to determine whether Vermi Cast could be used effectively to remove $SO_2$ from flue gas, and then to investigate optimum adsorption conditions. The Vermi Cast used as adsorbent was mechanically screened with 8~20 mesh sieve. The adsorption data for $SO_2$ were regressed using the Freundlich isotherm. The fit was generally satisfactory ($R^2$=0.945~0.982). With the temperature changes from 2$0^{\circ}C$ to 4$0^{\circ}C$, the constant k in Freundlich isotherm qe= $kCe^{1/n}$, decreased from 1.409 at 2$0^{\circ}C$to 0.297 at 4$0^{\circ}C$, and the exponent 1/n were decreased from 0.343 to 0.134. With the bed depth changes from 10cm to 30cm, the adsorption capacity expressed as mmol of $SO_2$ adsorbed per g of Vermi Cast increased from 0.247 to 0.381. Moisture content is an important parameter in the $SO_2$ adsorbed were observed over 0.3mmol $SO_2$ /g Vermi Cast. The best adsorption capacity was 0.487mmol $SO_2$ /g Vermi Cast, and it was obtained with moisture content 37%, temperature 2$0^{\circ}C$. From the above results, ti might be concluded that Vermi Cast is effectively as a good adsorbent to remove $SO_2$ from flue gas.

• Journal of Nutrition and Health
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• 제33권2호
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• pp.179-185
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• 2000

This study investigated the nutritional status of 33 hemodialysis patients. Their weight, height, triceps and mid-arm circumference were measured and their dietary intake and blood profiles evaluated. The subjects were 57.1$\pm$11.9 years old. The energy intakes of men and women were 61% and 68% of RDA, respectively. The men and women's intake of protein was 0.93g/IBWkg/d and 0.99g/IBWkg/d, respectively. According to the distribution of BMI, 22.2% of the men and 73.3% of the women were underweight. A total of 5.6% of the men were overweight, versus none of the women. The serum albumin levels of the men and women were 3.56 and 3.52g/dl, respectively. The serum cholesterol levels of the men and women were 134.1 and 148.5mg/dl, respectively. The subjects were divided into three groups according to the level of albumin, and their intakes of nutrients were compared with one another. The group with high levels of albumin did not show higher energy and protein intake than the other groups but the serum total protein level was significantly higher. When the nutritional status of the patients was evaluated by weight and serum albumin level, 6.0% of them showed kwashiorkor-type malnutrition and 75.8% of them showed mild malnutrition.