• Journal of Bio-Environment Control
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• v.1 no.2
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• pp.123-134
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• 1992

In order to verify the usability of the greenhouse for aquaculture with nutrient culture synchronously and to obtain the fundamental data fir the establishment of efficient farming technology, the characteristics of microclimate and the growth of leafy vegetables were examined. Tilapia averaged 428.6 g grew to 784 g(1.83 times) for 147 days from May 29 to Oct. 21 and fingerlings averaged 12.9 g grew by 1.37 times for 61 days from Sep. 13 to Nov. 12. The growth of vegetables such as water dropwort, leaf lettuce, Chinese cabbage, and Welsh onion in the greenhouse was better for aquaculture with nutrient culture than for nutrient culture only. Between above two greenhouses, pH and EC of nutrient solution was same but the temperature different by about 2$^{\circ}C$. Average day temperature, relative humidity, and $CO_2$ concentration were higher by 2.9$^{\circ}C$, 6%, and 200 ppm in the greenhouse for aquaculture with nutrient culture, respectively. Net assimilation rate of vegetables in the greenhouse was a little higher for aquaculture with nutrient culture than for nutrient culture only. Therefore, provided aquaculture and nutrient culture are carried out in the same greenhouse, the saving effect of heating cost as well as the additional promotive effects of vegetable and tilapia growth can be obtained.

• Journal of Plant Biotechnology
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• v.44 no.2
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• pp.125-134
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• 2017

Enhanced disease susceptibility1 (EDS1) is a regulator of basal defense responses required for resistance mediated by TIR-NBS-LRR containing R proteins. We identified three transcripts of EDS1-like genes encompassing diverse/separate expression patterns, based on the transcriptome analysis by Next Generation Sequencing (NGS) of V. flexuosa inoculated with Elsinoe ampelina. These genes were designated VfEDL1 (Vitis flexuosa Enhanced Disease Susceptibility1-like1), VfEDL2 and VfEDL3, and contained 2464, 1719 and 1599 bp, with 1791, 1227 and 1599 bp open reading frames (ORFs), encoding proteins of 596, 408 and 532 amino acids, respectively. The predicted amino acid sequences of all three genes showed the L-family lipase-like domain (class 3 lipase domain), and exhibited a potential lipase catalytic triad, aspartic acid, histidine and serine in the conserved G-X-S-X-G. All three VfEDL genes were upregulated at 1 hpi against the bacterial and fungal pathogens Rizhobiumvitis and E. ampelina, respectively, except VfEDL1, which was downregulated against E. ampelina at all time points. Against E. ampelina, VfEDL2 and VfEDL3 showed downregulated expression at later time points. When evaluated against R. vitis, VfEDL1 showed downregulated expression at all time points after 1 hpi, while VfEDL3 showed upregulation up to 24 hpi. Based on the expression response, all three genes may be involved in plant resistant responses against R. vitis, and VfEDL2 and VfEDL3 show additional resistant responses against E. ampelina infection.

• Journal of Environmental Health Sciences
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• v.43 no.4
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• pp.267-272
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• 2017

Objectives: The purposes of the study were to analyze the temporal variation of carbon dioxide ($CO_2$) and particulate matter (PM) in daycare centers and evaluate the appropriateness of the official test method of one-time measurement. Methods: Indoor air quality in 46 daycare centers in the Seoul Metropolitan Area was measured as specified in the official test method of Indoor Air Quality Management law. In addition, indoor air quality in the 46 daycare centers was measured over 37 days using a real-time monitor (AirGuard K). Results: The daily means of $CO_2$ and PM in the 46 daycare centers were $1042.74{\pm}134.45ppm$ and $67.60{\pm}18.25{\mu}g/m^3$, respectively. Indoor air quality in the daycare centers showed significant temporal fluctuation. Measurements for single days were significantly different from the 37-day average exposure. Relative error of short term exposure decreased with an increase in the number of sampling days. The noncompliance rate for $CO_2$ using the official testing method was 2.17%, and none exceeded the $PM_{10}$ standard of $100{\mu}g/m^3$. With monitoring over 37 days, the daily noncompliance rate for $CO_2$ was 50.4% and the daily noncompliance rate for PM was 13.8%. Conclusions: When the official test method evaluates the indoor air at daycare centers one day per year, the results may not represent actual indoor air quality over a longer period of time. Real-time monitoring devices could be an alternative for managing indoor air quality.

• Journal of Soil and Groundwater Environment
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• v.20 no.6
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• pp.73-84
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• 2015

Nine plant species were selected through vegetation survey at three military shooting ranges at northern Gyeonggi Province. Plants were germinated in normal soil and three seedlings were transplanted to a bottom sealed pot containing sandy loam soils contaminated with either RDX (291 mg/kg) or TNT (207 mg/kg). Planted, blank (without plant), and control (without explosive compound) pots were grown in triplicate at a green house for 134 days. During cultivation, transplanted plants exhibited chlorosis and necrosis in flower and leaf by explosive toxicity and stress. Only three plants, Wild soybean, Amur silver grass, Reed canary grass, survived in TNT treated pot, while seven plant species except for field penny cress and jimson weed, thrived in RDX treated pot. Appreciable amount of TNT (61.6~241.2 mg/g-D.W.) was detected only in plant roots. Up to 763.3 mg/g-D.W. along with 4-amino-2,6-dinitrotoluene, an intermediate of TNT, accumulated in the root of wild soybean. In addition, azoxy compounds, abiotic intermediates of TNT, were detected in TNT treated soils. RDX absorbed average 1,839.95 mg/kg in shoot and 204.83 mg/kg in root. Most of TNT in plant was accumulated in underground part whereas RDX was localized in aerial part. Material balance calculation showed that more than 95% of the initial TNT was removed in the planted pots whereas only 60% was removed in the blank pot. The amount of RDX removed from soil was in the order of Amur Silver Grass (51%) > Chickweed (43%) > Evening primrose (38%). Based on the results of pot cultures, Amur silver grass and Reed canary grass are selected as tolerant remedial plants for explosive toxicity.

• Bulletin of the Korean Chemical Society
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• v.32 no.6
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• pp.2008-2014
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• 2011

Serotonin or 5-hydroxytryptamine subtype 2C ($5-HT_{2C}$) receptor belongs to class A amine subfamily of G-protein-coupled receptor (GPCR) super family and its ligands has therapeutic promise as anti-depressant and -obesity agents. So far, bovine rhodopsin from class A opsin subfamily was the mostly used X-ray crystal template to model this receptor. Here, we explained homology model using beta 2 adrenergic receptor (${\beta}$2AR), the model was energetically minimized and validated by flexible ligand docking with known agonists and antagonists. In the active site Asp134, Ser138 of transmembrane 3 (TM3), Arg195 of extracellular loop 2 (ECL2) and Tyr358 of TM7 were found as important residues to interact with agonists. In addition to these, V208 of ECL2 and N351 of TM7 was found to interact with antagonists. Several conserved residues including Trp324, Phe327 and Phe328 were also found to contribute hydrophobic interaction. The predicted ligand binding mode is in good agreement with published mutagenesis and homology model data. This new template derived homology model can be useful for further virtual screening based lead identification.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.43 no.4
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• pp.345-350
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• 2010

We studied the organisms attached to Sargassum beds in Gamak Bay from November 2007 to June 2008. The water temperature ranged from 3.3 to $28.7^{\circ}C$ and salinity from 30.6 to 33.8 psu. Eighteen species $143,432\;ind./m^2$ were sampled during study periods. The biomass of the Sargassum beds ranged from a highest of $196.08\;g\;dw/m^2$ in March to a lowest of $0\;g\;dw/m^2$ in July. The major dominant species were Barleeia angustata ($134,430\;ind./m^2$), Caprellidae sp.($2,443\;ind./m^2$) and Gammaridae sp.($4,201\;ind./m^2$). The ecological indices of diversity, evenness, and richness were 0.11-0.86, 0.04-0.39, and 0.74-1.06, respectively.

• Animal cells and systems
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• v.11 no.2
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• pp.129-134
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• 2007

The initiation of eukaryotic DNA replication requires assembly of the pre-replicative complex (Pre-RC) through the concerted action of Orc, Cdc6, Cdt1 and Mcm2-7 complex during G1 phase. The pre-RC assembly licenses individual replication origins for the initiation of DNA replication and sufficient number of the pre-RC is essential for proper progression of S phase. However, it is not well known how cells recognize the completion of the pre-RC assembly before G1-S transition. In order to understand the cellular responses to the defects in pre-RC assembly, we depleted the known components of pre-RC proteins using the small interference RNAs in HeLa cells. Although the defects of pre-RC assembly by the depletion of the pre-RC proteins such as Orc2, Cdt1, Mcm2 & Mcm10 did not elicit the activation of Chk1- or Chk2-dependent checkpoint pathways, these cells still showed significant decrease in the cellular level of Cdc25A proteins. These results suggests that a novel checkpoint pathway exist in HeLa cells, which is not dependent upon Chk1 or Chk2 proteins and play essential roles in the cellular responses to the defects in the pre-RC assembly. Also, among those four proteins tested in this study, the depletion of Mcm10 and Cdt1 proteins significantly increased the apoptotic cell death in HeLa cells, suggesting that these proteins not only play roles in the pre-RC assembly, but also are involved in the checkpoint responses to the defects in the pre-RC assembly.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.41 no.4
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• pp.271-278
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• 2005

In order to study the catch situation of the octopus pot fishery in the coastal waters of Gangneung, catch quantities and weight per month and depth, the soaking time & loss of fishing gear were studied based on 62 fishing times (1 set consisted of 150 pots which is equivalent to 1 fishing time) over a period of 10 months from February to December 2003 using commercial fishing vessels and training ships. The monthly CPUE was generally high in the months of june - September, and during this period July showed the highest quantity (134g/pot, 19.21kg/haul). In consideration of the fact that octopus below the weight of 2kg is protected in foreign countries, the majority of the catch was small with 76% (486 octopuses) below 1kg. The octopus catch quantity was high in depth of more than 40m, and the average catch number per set of pots in depths of over 50m was the highest with 13 octopuses. CPUE(g/pot) was the highest at a depth of over 60m recording 101. In terms of catch quantity per soaking time (4-18 days) the highest was on the 7th day, and 13-18 days also recorded high catches, and this suggests the possibility of ghost - fishing with the loss of fishing gear. Loss of fishing gear was the highest in terms of loss frequency and amount at depths of 20-30m. Consequently, it seems that octopus pot fishing at depths of more than 30m would relieve friction with other types of fishing and increase fishing efficiency.

• Journal of the East Asian Society of Dietary Life
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• v.18 no.1
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• pp.127-134
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• 2008

This study was designed to evaluate the nutritional status and dietary problems of obese elementary students consuming unbalanced diets. Blood parameters, body-image perceptions, taste preferences, and nutrient intakes of 40 obese elementary students with unbalanced diets were compared to those of 40 additional obese elementary students as a control. The averages for age, height, weight, and obesity index were 11.5 years, 142.0 cm, 51.0 kg, and 35.4% in the study group and 11.8 years, 144.0 cm, 53.5 kg, and 36.3% in the control group, respectively. The major disliked foods by the students in the study group were vegetables (65%) particularly Kimchi (15%), as well as fish(15%), and pulses (10%). Mean serum cholesterol, blood glucose, GOT/GPT, and hemoglobin levels were 175.1 mg/dL, 89.2 mg/dL, 24.1/18.9 IU/L, and 14.3 g/dL in the study group and 182.8 mg/dL, 91.3 mg/dL, 28.3/33.9 IU/L, and 14.7 g/dL, in the control group, respectively. Approximately 60${\sim}$80% of the subjects responded that they eat a meal everyday, and the remainder skipped at least one meal. The preference for salty tasting foods was significantly lower in the study group, but the preference for hot tasting food was higher as compared to the obese control group(p<0.05, p<0.05). The daily energy intakes of the study and control groups were 1,768.3 kcal and 1,572.7 kcal, respectively, but there was no significant differences between them. Daily intakes of animal protein, fat, animal fat, and vitamin $B_2$ were significantly higher in the study group than in the control group. In conclusion, this study found that obese children consuming unbalanced diets strongly disliked vegetables, particularly Kimchi, as well as hot tasting foods; however, they preferred salty foods. Daily intakes of animal protein and fat were higher in the study group as compared to obese children not consuming unbalanced diets.

• Journal of the Korean Wood Science and Technology
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• v.30 no.4
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• pp.33-40
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• 2002

This research was to investigate the adsorption characteristics of charcoals of major Korean wood species (thinned trees) and wood-based materials. As carbonization time and temperature increased, methylene-blue adsorption (MBA) of charcoals of thinned trees and wood-based materials increased. Pinus koraiensis charcoal showed the highest MBA. MBA of softwoods was higher than that of hardwoods. There was a negative correlation between air-dried specific gravity before carbonization and MBA of carbonized thinned trees. The MBA of wood-based materials showed similar value, but wood-based materials in MBA was lower than thinned woods. Surface area and total pore volume of thinned trees and wood-based materials increased as carbonization temperature increased. The species showing highest MBA appeared to have the highest surface area at the carbonization temperature of 600℃ as well. There was a positive correlation between surface area, total pore volume and MBA of charcoals. The charcoal of wood-based materials generally exhibited micro pores.