• Title/Summary/Keyword: G134A

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Identification and Characterization of Phytochemicals from Peanut (Arachis hypogaea L.) Pods

  • Lee, Jin-Hwan;Baek, In-Youl;Ha, Tae-Joung;Choung, Myoung-Gun;Ko, Jong-Min;Oh, Sea-Kwan;Kim, Hyun-Tae;Ryu, Hyung-Won;Park, Keum-Yong;Park, Ki-Hun
    • Food Science and Biotechnology
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    • v.17 no.3
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    • pp.475-482
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    • 2008
  • Methanol extracts of peanut (Arachis hypogaea L.) pods were chromatographed, which yielded 3 phytochemicals 1-3 including 5,7-dihydroxychromone (1), eriodictyol (2), and 3',4',5,7-tetrahydroxyflavone (3). To confirm the presence of isolated phytochemicals, the pods extracts were performed by high performance liquid chromatography coupled with a photodiode array detector (HPLC-PDA) and a mass spectrometric detector (MSD) with electrospray ionization (ESI). Optimum extraction conditions for phytochemical contents using peanut germplasm were obtained by employing 90% MeOH for 12 hr at room temperature and phytochemicals 1-3 showed significant differences with concentrations of $407.56{\pm}23.35$, $52.92{\pm}5.11$, and $2,024.34{\pm}134.18\;{\mu}g/g$, respectively. Under this optimal conditions, the contents of phytochemicals 1-3 in peanut pods of 3 Korea cultivars including 'Jakwang', 'Daekwang', and 'Palkwang' exhibited phytochemical 3 was the highest range of $1,338.01-5,162.93\;{\mu}g/g$, followed by phytochemical 1 ($590.13-1,382.10\;{\mu}g/g$), and phytochemical 2 ($25.12-186.85\;{\mu}g/g$), respectively. Moreover, 'Jakwang' exhibited the highest contents of phytochemical (1: $1,362.10{\pm}52.49$, 2: $186.85{\pm}17.69$, and 3: $5,162.93{\pm}148.64\;{\mu}g/g$, respectively), whereas the lowest contents was found in the 'Daekwang' (1: $590.13{\pm}22.23$, 2: $25.12{\pm}2.45$, and 3: $1,338.01{\pm}62.17\;{\mu}g/g$, respectively). These results suggest that the methanol extracts of peanut pods may possess health related benefits to humans owing to various known biological activities of phytochemicals 1-3.

Learning Flow, Self-Directedness, Self-Regulated Learning Ability and Learning Achievement of Nursing Students who in Non-Face-To-Face Learning Environment (비대면 학습환경에서 간호대학생의 학습몰입, 자기 주도성, 자기조절학습능력 및 학업성취도)

  • Lee, Ji Eun
    • The Journal of the Convergence on Culture Technology
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    • v.7 no.4
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    • pp.511-517
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    • 2021
  • This study is a descriptive investigative study to correlate of learning flow, self-directedness, self-regulated learning ability and learning achievement of nursing students who in non-face-to-face learning environment. The subjects of the study were 179 nursing college students based in B, G city, who collected data from September to October 2021. The data analysis used the SPSS 24.0 program and performed descriptive statistics and correlation analysis. As a result of the study, the average learning flow was 38.54±7.87 points, self-directedness was 134.83±19.81 points, self-regulated learning ability was 42.93±5.98 points and learning achievement was 15.03±2.56 points. The relationships among learning flow, self-directedness, self-regulated learning ability, and learning achievement showed significant positive correlations. Therefore, strategies to increase the learning achievement of nursing college students in a non-face-to-face environment, and environmental improvement and institutional support to increase learning flow are needed.

Kinetics of Cell Growth and Cyclosporin A Production by Tolypocladium inflatum when Scaling Up from Shake Flask to Bioreactor

  • El Enshasy, H.;Fattah, Y. Abdel;Atta, A.;Anwar, M.;Omar, H.;Magd, S. Abou El;Zahra, R. Abou
    • Journal of Microbiology and Biotechnology
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    • v.18 no.1
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    • pp.128-134
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    • 2008
  • The kinetics of cell growth and Cyclosporin A (Cyc A) production by Tolypocladium inflatum were studied in shake flasks and bioreactors under controlled and uncontrolled pH conditions. In the case of the shake flask, the production time was extended to 226 h and the maximal antibiotic concentration was 76 mg/l. When scaling up the cultivation process to a bioreactor level, the production time was reduced to only 70h with a significant increase in both the cell growth and the antibiotic production. The maximal dry cell weights in the case of the controlled pH and uncontrolled pH cultures in the bioreactor were 22.4g/l and 14.2g/l, respectively. The corresponding maximal dry cell weight values did not exceed 7.25g/l with the shake flask cultures. The maximal values for Cyc A production were 144.72 and 131.4 mg/l for the controlled and uncontrolled pH cultures, respectively. It is also worth noting that a significant reduction was observed in both the dry cell mass and the antibiotic concentration after the Cyc A production phase, whereas the highest rate of antibiotic degradation was observed in the stirred tank bioreactor with an uncontrolled pH. Morphological characterization of the micromorphological cell growth (mycelial/pellet forms) was also performed during cultivation in the bioreactor.

Development of a Fiber-Optic Biosensor for the Detection of Listeria monocytogenes (리스테리아 식중독균 검출을 위한 광학식 바이오센서 개발)

  • Kim G.;Choi K.H.
    • Journal of Biosystems Engineering
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    • v.31 no.2 s.115
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    • pp.128-134
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    • 2006
  • Frequent outbreaks of foodborne illness demand the need for rapid and sensitive methods for detection of these pathogens. Recent development of biosensor technology has a great potential to meet the need for rapid and sensitive pathogens detection from foods. An antibody-based fiber-optic biosensor and an automated reagents supply system to detect Listeria monocytogenes were developed. The biosensor for detection of Listeria monocytogenes in PBS and bacteria spiked food samples was evaluated. The automated reagents supply system eliminated cumbersome sample and detection antibody injection procedures that had been done manually. The biosensor could detect $10^4$ cfu/ml of Listeria monocytogenes in PBS. By using the fiber-optic biosensor, $2x10^8$ cfu/ml of Listeria monocytogenes in the food samples were detectable.

Electrochemical Properties of LiNi0.4Mn0.3Co0.3O2 Cathode Material for Lithium Ion Battery (리튬이온전지용 정극활물질 LiNi0.4Mn0.3Co0.3O2의 전기화학적 특성)

  • Kong, Ming-Zhe;Kim, Hyun-Soo;Kim, Ke-Tack;Gu, Hal-Bon
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.19 no.7
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    • pp.650-654
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    • 2006
  • [ $LiNi_{0.4}Mn_{0.3}Co_{0.3}O_2$ ] cathode material was synthesized by a mixed hydroxide method. Structural characterization was carried out using X-ray diffraction studies. Electrochemical studies were performed by assembling 2032 coin cells with lithium metal as an anode. DSC (Differential scanning calorimetry) data showed that exothermic reactions of $LiNi_{0.4}Mn_{0.3}Co_{0.3}O_2$ charged to 4.3 V versus Li started at high temperatures$(280\sim390^{\circ}C)$. The cell of $LiNi_{0.4}Mn_{0.3}Co_{0.3}O_2$ mixed cathode delivered a discharge capacity of 150 mAh/g at a 0.2 C rate. The capacity of the cell decreased with the current rate and a useful capacity of 134 mAh/g was obtained at a 2 C rate. The reversible capacity after 100th cycles was 126 mAh/g when a cell was cycled at a current rate of 0.5 C in $2.8\sim4.3V$.

A Study on the Amount and Major Compositions of Excreta from Broilers (육계의 분뇨 배설량 및 분뇨 성분 조사 결과)

  • HwangBo, J.;Hong, E.C.;Chung, I.B.;Kang, K.H.;Park, H.D.;Suh, O.S.;Chung, W.T.;Jang, S.K.
    • Korean Journal of Poultry Science
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    • v.36 no.2
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    • pp.157-163
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    • 2009
  • This work was conducted to investigate the amount and major compositions of excreta from broilers. One hundred fifty 1-d-old male and female Ross broilers were used in this work and ten broilers were selected to measure excreta. Average amount of excreta per broiler was 85.4 g, and average moisture content was 76.9%. N, $P_2O_5$ and $K_2O$ in excreta were 1.19, 0.29 and 0.49%, respectively. pH, $BOD_5$, $COD_{mn}$, and SS were 7.80, 42,134 mg/L, 52,668 mg/L, and 106,669 mg/L, respectively. Pb in poultry excreta was 0.803 mg/kg and highly lowed compared with the standard contents (150 mg/kg). Cd was not founded in excreta. Hg was founded in excreta as 0.002 mg/kg, but it was low compared with the standard level (2 mg/kg).

Synthesis of Multiwall Carbon Nanotube/Graphene Composite by Aerosol Process and Its Characterization for Supercapacitors (에어로졸 공정에 의한 Multiwall carbon nanotube/Graphene 복합체 제조 및 슈퍼커패시터 특성평가)

  • Jo, Eun Hee;Kim, Sun Kyung;Chang, Hankwon;Lee, Chong Min;Park, Su-Ryeon;Choi, Ji-hyuk;Jang, Hee Dong
    • Particle and aerosol research
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    • v.12 no.4
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    • pp.127-134
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    • 2016
  • A multiwall carbon nanotube (MWCNT)/graphene (GR) composite was synthesized for an enhanced supercapacitor. Aerosol spray pyrolysis (ASP) was employed to synthesize the MWCNT/GR composites using a colloidal mixture of MWCNT and graphene oxide (GO). The effect of the weight ratio of the MWCNT/GO on the particle properties including the morphology and layered structure were investigated. The morphology of MWCNT/GR composites was generally the shape of a crumpled paper ball, and the average composite size was about $5{\mu}m$. MWCNT were uniformly dispersed in GR sheets and the MWCNT not only increase the basal spacing but also bridge the defects for electron transfer between GR sheets. Thus, it was increasing electrolyte/electrode contact area and facilitating transportation of electrolyte ion and electron in the electrode. Electrochemical data demonstrate that the MWCNT/GR (weight ratio=0.1) composite possesses a specific capacitance of 192 F/g at 0.1 A/g and good rate capability (88% capacity retention at 4 A/g) using two-electrode testing system.

Antioxidant and Antibacterial Activities of the Byproducts of Abies holophylla Extract (전나무 부산물 추출물의 항산화 및 항박테리아 활성)

  • Seong, Eun Soo;Kim, Soo Kyung;Lee, Jin Won;Choi, Seung Hyuk;Yoo, Ji Hye;Lim, Jung Dae;Na, Jong Kuk;Yu, Chang Yeon
    • Korean Journal of Medicinal Crop Science
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    • v.26 no.2
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    • pp.134-140
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    • 2018
  • Background: The extract of Abies holophylla is used as an ingredient in cosmetics. This study assessed the antioxidant and antibacterial activities of the material remaining after the extract is used. Methods and Results: The 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis (3-ethyl benzothiazoline)-6-sulfonic acid (ABTS) radical scavenging abilities were assessed to determined the free radical scavenging activity. The total phenol and flavonoid contents were determined to measure the antioxidant activity. The DPPH and ABTS radical scavenging activities of the resudual extract were higher (95.61 - 99.42% and 74.26 - 77.98% in water extract respectively) than those of the positive control. In 50% EtOH extract, the total phenol content was $389.84mg{\cdot}GAE/m{\ell}$, and the total flavonoid was $0.15mg{\cdot}QE/m{\ell}$. The minimum inhibition concentration degree for antibacterial activity against Staphylococcus aureus was < 8 to < $125{\mu}g/m{\ell}$ compared to that of the positive control in all extracts. The clear zone against S. aureus was found to be $12.2{\pm}3.8mm$. Conclusions: The A. holophylla byproducts were found to have antioxidant and antibacterial activities. Therefore, the materials remaining after the A. holophylla extract is used in cosmetics has potential functional uses.

Dolichos biflorus Linn attenuate progression of renal damage in alloxan-induced diabetic rats

  • Chidrewar, G.U.;Mane, V.S.;Ghaisas, M.M.;Deshpande, A.D.
    • Advances in Traditional Medicine
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    • v.10 no.2
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    • pp.134-140
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    • 2010
  • Dolichos biflorus Linn. (Fabaceae), commonly known as Horse gram is a medicinal plant, used in folk medicine for treating kidney stones and diabetes mellitus. The purpose of the present study was to investigate the effects of daily oral feeding of various doses of methanolic extract of Dolichos biflorus seeds (DB) for 42 days on blood glucose concentrations and kidney functions in Alloxan-diabetic rats. Plasma glucose levels, body weight, serum creatinine, and urinary albumin levels were monitored on $15^{th}$, $29^{th}$, $43^{rd}$ day. Renal hypertrophy was assessed as the ratio between the kidney weight and body weight of the rats. Plasma glucose concentrations in Alloxan-diabetic rats were significantly reduced by the administration of DB (350 mg/kg) and DB (700 mg/kg) on day 15 and onwards (P < 0.01). After 15 days of Alloxan administration urinary albumin levels (UAE) were over 5 fold higher in diabetic controls as compared to normal controls. Treatment with DB significantly prevented the rise in UAE levels from day 15 to 43 in comparison to diabetic controls (P < 0.01). Renal hypertrophy was significantly higher in diabetic controls as compared to non-diabetic controls. Treatments with DB (350 mg/kg) and DB (700 mg/kg) significantly prevented renal hypertrophy (P < 0.01) as compared to diabetic controls. DB (175 mg/kg) failed to modify renal hypertrophy. Thus the present study indicates that methanolic extract of Dolichos biflorus may be useful in management of hyperglycemia and kidney functions in Alloxan-diabetic rats.