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Optimization of Xylitol Production by Candida tropicalis in Two-stage Fed-batch Culture (Candida tropicalis의 2단계 유가식 배양에 의한 Xylitol 생산의 최적화)

  • 유연우;조영일;서진호
    • KSBB Journal
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    • v.17 no.1
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    • pp.93-99
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    • 2002
  • Two-stage fed-batch culture of Candide tropicalis that was designated primarily to cultivate the cell in the glucose medium (1st stage) and then produced the xylitol from xylose medium (2nd stage) was developed to improve a xylitol yield and productivity. In the growth stage, glucose was automatically supplied to the fermentor by pH-stat mode when the pH was up 5.7, When a feeding medium was added in order to reach the glucose and yeast extract concentrations up to 100 and 40 g/L, respectively, a high cell concentration and a relatively low ethanol concentration were obtained in 18.5 h culture. In the production stage, initial xylose concentration of 150 g/L was the most favorable for obtaining the final xylitol concentration and productivity. The addition of mineral salts was also enhanced a xylitol production. But the aeration rate was not significantly affected a xylitol production. When the addition of 16 g yeast extract and 232.5 g xylose powder at the production stage was used, xylitol yield and productivity were significantly increased. With these conditions, xylitol concentration, yield and productivity of 108.9 g/L, 74%) and 3.3 g/L·h, respectively, were obtained in a final volume of 1.58 L. The further addition of 16 g yeast extract and 232.5 g xylose powder increased the working volume partly (1.67 L) and resulted in a relatively high xylitol concentration, yield and productivity of 193 g/L, 70% and 3.6 g/L·h, respectively.

IPC Code Based Analysis of Technology Convergence of the IoT Patents in South Korea, China, and Japan : Focusing on PCT International Applications (한중일 사물인터넷(IoT) 관련 특허의 IPC 코드 기반 기술융복합 분석 : PCT 국제출원을 중심으로)

  • Shim, Jaeruen
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.24 no.7
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    • pp.949-955
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    • 2020
  • In this Study, Social Network Analysis of IoT related patents in South Korea, China, and Japan was conducted from the viewpoint of patent informatics. To this end, 2,526 patents filed by PCT until December 2019 were investigated up to the subclass level of the IPC code. As a result, in the case of South Korea, representative IPC codes are in the order of G06Q, H04L, G06F, H04W, and the highest frequency of interconnection is H04L→H04W, H04W→H04L, H04W→H04B. In China, the representative IPC codes are in the order of H04L, H04W, G05B, G06Q. South Korea has strong technological convergence centered on the G06Q, while China has strong convergence centered around H04L and H04W. Moreover, in China, H04L and H04W have more diverse combinations than in South Korea in Section A, B, G, and H. In the future, it is necessary to study the diversity of technology convergence of H04L and H04W in China.

The Effect of Potassium Phosphate as a pH Stabilizer on the Production of Gellan by Sphingomonas paucibilis NK-2000 (Sphingomonas paucibilis NK-2000에 의한 젤란의 생산에 미치는 pH 안정제로서의 인산칼슐의 영향)

  • Lee, Nam-Kyu;Jo, Young-Bae;Jin, Il-Hyuck;Son, Chang-Woo;Lee, Jin-Woo
    • Journal of Life Science
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    • v.19 no.8
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    • pp.1033-1038
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    • 2009
  • Maximal productions of gellan by Sphingomnas paucibilis NK2000 from 20 g/l glucose and 10 g/l soybean pomace were 7.46 g/l in a flask and 7.35 g/l in a 7 l bioreactor, when the initial pH of media was 6.8. Maximal production of gellan in a 7 l bioreactor under pH control by sodium hydroxide was 8.42 g/l, whereas that under control by potassium phosphate was 8.50 g/l. The optimal concentration of potassium phosphate in a medium for production of gellan by S. paucibilis NK2000 was found to be 5.0 g/l. Maximal production of gellan in a medium containing 5.0 g/l potassium phosphate without pH control was 8.93 g/l in a 7 l bioreactor. In this study, a simple process without pH control was developed to enhance the production of gellan, with optimized concentration of potassium phosphate in the medium.

Cultural Condition for Biopolymer Production by Pseudomonas delafieldii (Pseudomonas delafieldii에 의한 Biopolymer 생산조건)

  • Yoo, Jin-Young;Chung, Dong-Hyo
    • Microbiology and Biotechnology Letters
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    • v.17 no.5
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    • pp.468-474
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    • 1989
  • The cultural condition for polysaccharide production by Pseudomonus detafietdii was studied. The optimal medium contains the following composition per liter of distilled water: glucose (25g/$\ell$), peptone (2.06g/$\ell$), KH$_2$PO$_4$(2g/$\ell$), MgSO$_4$.7$H_2O$ (2g/$\ell$), yeast extract (0.5g/$\ell$), CaCO$_3$(2.5g/$\ell$). The temperature and pH optimum were 3$0^{\circ}C$ and 6.5. The agitation speed was 300 rpm. 5.91g of polysaccharide was produced at the condition in flask culture.

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THE MODULI SPACES OF LORENTZIAN LEFT-INVARIANT METRICS ON THREE-DIMENSIONAL UNIMODULAR SIMPLY CONNECTED LIE GROUPS

  • Boucetta, Mohamed;Chakkar, Abdelmounaim
    • Journal of the Korean Mathematical Society
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    • v.59 no.4
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    • pp.651-684
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    • 2022
  • Let G be an arbitrary, connected, simply connected and unimodular Lie group of dimension 3. On the space 𝔐(G) of left-invariant Lorentzian metrics on G, there exists a natural action of the group Aut(G) of automorphisms of G, so it yields an equivalence relation ≃ on 𝔐(G), in the following way: h1 ≃ h2 ⇔ h2 = 𝜙*(h1) for some 𝜙 ∈ Aut(G). In this paper a procedure to compute the orbit space Aut(G)/𝔐(G) (so called moduli space of 𝔐(G)) is given.

SHARP CONDITIONS FOR THE EXISTENCE OF AN EVEN [a, b]-FACTOR IN A GRAPH

  • Cho, Eun-Kyung;Hyun, Jong Yoon;O, Suil;Park, Jeong Rye
    • Bulletin of the Korean Mathematical Society
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    • v.58 no.1
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    • pp.31-46
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    • 2021
  • Let a and b be positive integers, and let V (G), ��(G), and ��2(G) be the vertex set of a graph G, the minimum degree of G, and the minimum degree sum of two non-adjacent vertices in V (G), respectively. An even [a, b]-factor of a graph G is a spanning subgraph H such that for every vertex v ∈ V (G), dH(v) is even and a ≤ dH(v) ≤ b, where dH(v) is the degree of v in H. Matsuda conjectured that if G is an n-vertex 2-edge-connected graph such that $n{\geq}2a+b+{\frac{a^2-3a}{b}}-2$, ��(G) ≥ a, and ${\sigma}_2(G){\geq}{\frac{2an}{a+b}}$, then G has an even [a, b]-factor. In this paper, we provide counterexamples, which are highly connected. Furthermore, we give sharp sufficient conditions for a graph to have an even [a, b]-factor. For even an, we conjecture a lower bound for the largest eigenvalue in an n-vertex graph to have an [a, b]-factor.

Fundamental Groups of a Topological Transformation Group

  • Chu, Chin-Ku;Choi, Sung Kyu
    • Journal of the Chungcheong Mathematical Society
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    • v.4 no.1
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    • pp.103-113
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    • 1991
  • Some properties of a path space and the fundamental group ${\sigma}(X,x_0,G)$ of a topological transformation group (X, G, ${\pi}$) are described. It is shown that ${\sigma}(X,x_0,H)$ is a normal subgroup of ${\sigma}(X,x_0,G)$ if H is a normal subgroup of G ; Let (X, G, ${\pi}$) be a transformation group with the open action property. If every identification map $p:{\Sigma}(X,x,G)\;{\longrightarrow}\;{\sigma}(X,x,G)$ is open for each $x{\in}X$, then ${\lambda}$ induces a homeomorphism between the fundamental groups ${\sigma}(X,x_0,G)$ and ${\sigma}(X,y_0,G)$ where ${\lambda}$ is a path from $x_0$ to $y_0$ in X ; The space ${\sigma}(X,x_0,G)$ is an H-space if the identification map $p:{\Sigma}(X,x_0,G)\;{\longrightarrow}\;{\sigma}(X,x_0,G)$ is open in a topological transformation group (X, G, ${\pi}$).

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The Effect of waste Oyster Shell on Anaerobic Composting of Garbage (굴 패각이 음식쓰레기의 혐기성 퇴비화에 미치는 영향)

  • Kim, Nam-Chan;Han, Se-Hyun
    • Journal of the Korea Organic Resources Recycling Association
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    • v.8 no.3
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    • pp.89-96
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    • 2000
  • Waste oyster shell was used to study the applicability on anaerobic composting of garbage. Experiment was conducted with five anaerobic reactors. After garbage and compost for inoculation were mixed, waste oyster shell was added into mixed waste in mesophillic temperature for 60 days with 30%, 60% respectively. The amount of produced methane gas, gas composition, organic removal efficiency and pH were measured. Result showed that the amounts of produced gas were 0.62 l /g-VS in R-1, 0.63 l /g-VS in R-2, 0.16 l /g-VS in R-3, 0.75 l /g-VS in R-4, 0.21 l /g-VS in R-5 and the amounts of produced methane gas were 0.32 l /g-VS in R-1, 0.37 l /g-VS in R-2, 0.04 l /g-VS in R-3, 0.42 l /g-VS in R-4, 0.05 l /g-VS in R-5. Proportion of mathane gas which determines the efficiency of anaerobic composting was over 55%. pH were 6.0~8.0 in R-2 and R-4 which contained 30% of waste oyster shell and pH were increased over 8.5 in R-3 and R-5 which contained 60% of waste oyster shell. Since pH were so high and it had a negative effect on microbial growth, anaerobic reaction were not well operated in R-3 and R-5.

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Lowered Substrate pH Reduced the Bicarbonate Injury during Vegetative Growth of 'Ssanta' Strawberry (혼합상토의 pH 저하가 영양생장 중인 '싼타' 딸기의 중탄산 피해 경감에 미치는 영향)

  • Lee, Hee Su;Cheung, Jong Do;Choi, Jong Myung
    • Journal of Bio-Environment Control
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    • v.26 no.2
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    • pp.115-122
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    • 2017
  • Objective of this research was to investigate the influence of lowered substrate pH on the reduction of bicarbonate injury in the vegetative growth of 'Ssanta' strawberry. The acid substrate was formulated by mixing sphagnum peat moss with pine bark (5:5, v/v) and the pH, EC, and CEC of the substrate were 4.07, $0.46dS{\cdot}m^{-1}$, and $91.3cmol^+{\cdot}kg^{-1}$, respectively. To adjust the pH of acid substrate, various amount of dolomitic lime [$CaMg(CO_3)_2$] were incorporated with the rate of 0 (untreated), 1, 2, 3, and $4g{\cdot}L^{-1}$. Then, mother plants were transplanted and grown with fertilizer solution containing $240mg{\cdot}L^{-1}$ of the $HCO_3{^-}$ and equal concentrations of essential nutrients to Hoagland solution. In growth of 'Ssanta' strawberry, fresh weight of mother plants were the highest in the treatment of $2g{\cdot}L^{-1}$ dolomitic lime such as 102.1 g followed by 94.7 g in $1g{\cdot}L^{-1}$, 91.2 g in $3g{\cdot}L^{-1}$, 75.7 g in $0g{\cdot}L^{-1}$ and 72.3 g in $4g{\cdot}L^{-1}$ treatments. The dry weight showed a similar tendency to fresh weight. At 140 days after transplanting, 5.8, 9.8, 11.8, 8.8, and 5.0 daughter plants were derived from each of the mother plants in the treatments of 0, 1, 2, 3, and $4g{\cdot}L^{-1}$ dolomitic lime, respectively. The highest occurrence of daughter plants were observed in the treatments $2g{\cdot}L^{-1}$ dolomitic lime. The substrate pH and bicarbonate concentration of 'Ssanta' strawberry seedlings in the 1 and $2g{\cdot}L^{-1}$ dolomitic lime treatments were maintained at a proper range such as 5.6 to 6.2. The micro-nutrient contents of above ground tissue in mother plants were the highest in $2g{\cdot}L^{-1}$ and the lowest in $4g{\cdot}L^{-1}$ dolomitic lime treatment. The above results indicate that incorporation rate of dolomitic lime in acid substrate with the pH of around 4 is $2g{\cdot}L^{-1}$ to raise the 'Ssanta' strawberry in propagation.

Evaluation for Rock Cleavage Using Distribution of Microcrack Spacings (I) (미세균열의 간격 분포를 이용한 결의 평가(I))

  • Park, Deok-Won
    • The Journal of the Petrological Society of Korea
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    • v.25 no.1
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    • pp.13-27
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    • 2016
  • The characteristics of the rock cleavage inherent in Jurassic granite from Geochang were analysed. The phases of distribution of microcrack spacings were derived from the enlarged photomicrographs(${\times}6.7$) of the thin section. The evaluation for the six directions of rock cleavages was performed using nine parameters such as (1) frequency of microcrack spacing(N), (2) frequency ratio(${\leq}1mm$ and 4 mm >) to total spacing frequency(N:191), (3) spacing ratio(${\leq}1mm$) to total spacing(118.49 mm), (4) mean spacing($S_{mean}$), (5) difference value($S_{mean}-S_{median}$) between mean spacing and median spacing($S_{median}$), (6) density of spacing, (7) median spacing, (8) reduction ratio of spacing frequency to length frequency and (9) magnitude of exponent(${\lambda}$ and b) related to the distribution type of diagram. Especially the close dependence between the above spacing parameters and the parameters from the spacing-cumulative frequency diagrams was derived. The results of correlation analysis between the values of parameters for three rock cleavages and those for three planes are as follows. The values of (I) parameters(1, 2 and 3), (II) parameters(4, 5 and 6), (III) parameter(7), (IV) parameter(8) and (V) parameter(9) show the various orders of H(hardway, H1+H2) < G(grain, G1+G2) < R(rift, R1+R2), R < G < H, R < H < G, G < H < R and H < G < R, respectively. On the contrary, the values of the above four groups(I~IV) of parameters for three planes show reverse orders. This type of correlation analysis is useful for discriminating three quarrying planes. Six spacing-cumulative frequency diagrams were arranged in increasing order on the value of main parameter($S_{mean}-S_{median}$). These diagrams show an order of R2 < R1 < G2 < G1 < H2 < H1 from the related chart. In other words, the above six diagrams can be summarized in order of rift(R1+R2) < grain(G1+G2) < hardway(H1+H2). These results indicate a relative magnitude of rock cleavage related to microcrack spacing. Especially, the above main parameter could provide advanced information for prediction the order of arrangement among the diagrams.