• Korean Journal of Microbiology
• /
• v.37 no.1
• /
• pp.28-36
• /
• 2001

These studies were carried out to understand the mechanisms of genes which are related in cell cycle progression at G1/S phase. Mutants involved in cell cycle progression and regulation in Saccharomyces cerevisiae were isolated and characterized. To isolate new mutants, we screened the sensitivity to ciclopirox olamine (CPO) which inhibits the cell cycle traverse at or very near the G1/S phase boundary in HeLa cell and budding yeast. As results, we isolated 30 mutants and named cos(ciclopirox olamine sensitivity: cos27∼cos57) mutants. To determine the phenotype of mutants, we examined the sensitivity to methyl-methane sulfonate (MMS) and hydroxyurea (HU). Several mutants were sensitive to MMS and HU. According to these Phenotypes, cos mutants were grouped into four. Group I mutants are cos27, cos28, cos32, cos33, cos36, cos37, cos40, cos42, cos46, cos50, cos52 and cos53 which show MMS, HU sensitivities and might act at a checkpoint pathway during S phase. Group II mutants are cos43 and cos48 which show MMS sensitivities and might act at a checkpoint pathway during Gl or G2 phase. Group III mutants are cos35, cos47, cos54, cos55 and cos56 which show HU sensitivities and might act at a progress pathway during S phase. Finally, Group IV mutants are cos29, cos30, cos31, cos34, cos38, cos39, cos41, cos44, cos45, cos49, cos51 and cos57 which show only CPO sensitivities. Moreover, we examined the terminal phenotype of mutants under fluorescent microscope and then found one of S phase checkpoint related mutant(cos37). Furthermore, we constructed the heterozygote strain between mutant and wild type haploid strains to study their genetic analysis of cos mutants.

• Korean Journal of Veterinary Research
• /
• v.22 no.2
• /
• pp.139-147
• /
• 1982

A total of 133 isolates of yeast-like fungi was tested for sensitivity to seven different antifungal agents. The yeast-like fungi tested were isolated from the milk from normal or mastitic bovine quaters or from bovine feces. They were 5 Candida albicans (C. albicans) isolates, 63 C. krusei, 27 C. tropicalis, 5 C. parapsilosis, 10 Torulopsis glabrata, 6 Rhodotorula sp., 6 Hansenula sp. and 1 Pichia sp. isolate. The antifungal agents tested were nystatin, griseofulvin, cycloheximide, 5-fluorocytosine, miconazol, clotrimazole and tolnaftate. In general, clotrimazole, miconazol and 5-fluorocytosine were more effective in antifungal activity in vitro against the test organisms than the rest of the agents tested. However, some of the isolates showed higher resistance to certain antifungal agents compared to the other isolates of the some species. They were: 1 C. albicans isolate to 5-fluorocytosine; 1 C. albicans to 5-fluorocytosine, miconazol and clotrimazole; 1 C. krusei to 5-fluorocytosine and cycloheximide; and 11 C. tropicalis isolates to cycloheximide. The minimum inhibitory concentrations(MIC) of clotrimazole were $12.5{\mu}g/ml$ or lower for all isolates tested except one C. albicans isolate, for which MIC of the drug was $100{\mu}g/ml$. On the other hand, the MIC's of cycloheximide were $6.5{\mu}g/ml$ or lower for all isolates except the following; all isolates of C. albicans ($100{\mu}g/ml$), C. pseudotropicalis ($200{\mu}g/ml$) and Rhodotorula sp. ($25-50{\mu}g/ml$), 11 C. tropicalis isolates ($100{\mu}g/ml$) and 1 C. krusei isolate ($200{\mu}g/ml$).

• Advances in Traditional Medicine
• /
• v.6 no.4
• /
• pp.306-311
• /
• 2006

This communication emphasized upon the sensitivity of the crude extracts of clerodendrum inerme against some of the human pathogenic bacteria. Five plant extracts (Petrol, Benzene, Methanol, Ethly acetate and Aqueous) under six different concentrations(500 ${\mu}g/ml$, 1 mg/ml, 2 mg/ml, 5 mg/ml, 10 mg/ml and 15 mg/ml) were tested by disk diffusion method. Methanol, Ethyl acetate and Aqueous extracts of the plant showed significant inhibition against fifteen of the eighteen bacteria tested. No earlier report on antibacterial activity of this taxon could be found in literature.

• Journal of Photoscience
• /
• v.9 no.2
• /
• pp.466-469
• /
• 2002

An experiment has been conducted to measure the impact of UV radiation sensitivity on dermatophytes (Microsporum boullardii) by different UV radiation exposure time interval (1 min, 2 min 5 min, 10 min and 20 min) in degradation of keratin (Feather) in growth promoting substances of protein, cysteine, cystine and methionine from 7 to 28 days of incubation period. Mutant strain caused maximum weight loss with 1 minutes of UV radiation exposure at 21 day and mutant strain became immune in sensitivity at 14 days for decomposition of feathers. Maximum protein caused at 21st days with 20 minutes U.V radiation exposure and immune sensitivity had deducted with other UV radiation exposure time. On 28 days, mutant strains became immune with all exposure times, Whereas maximum methionine caused at 21st days with 20 minutes UV radiation exposure. Maximum cysteine caused at $14^{th}$ day with 5 minutes UV radiation exposure and mutant strain showed immune response at all time periods. Cystine production was also followed by cysteine at 21 day and also showed complete immune response with 1 and 2 minutes UV radiation exposure at7 and 14 days. Thus mutant strain of Microspornm boullardii can be used as a biotechnological tool for production of growth promoting substances.

• Proceedings of the KIEE Conference
• /
• 1996.07c
• /
• pp.1713-1715
• /
• 1996

The $NO_2$ GAS Sensitivity characteristic of CuTBP(Copper-tetra-tert-butylphthalocyanine) LB films were investigated through a study of current-voltage (I-V) characteristics with a variation of number of electrode finger pairs N ($1{\sim}25$). A concentration of 200ppm $NO_2$ gas was used. It was found that a conductance G increases monotonically as the number of interdigital electrode increases, and a Sensitivity, Reproducibility is stable. As far as a current is concerned, the current when N=25 is greater than that when N=1 by 70 or so. It indicates that the number of interdigital electrodes affects the current, sensitivity and stability. We knew that the $NO_2$ gas detector application possibility using a current of N=25.

• Transactions of the Korean Society of Automotive Engineers
• /
• v.16 no.6
• /
• pp.115-120
• /
• 2008

There are many typesof accelerometer sensor. There was mainly used high-g accelerometer to obtain data for vehicle in crash test. Accelerometer was mounted on test vehicle with mounting blocks. Test result can be influenced by condition of mounting i.e. bonding material and type of block. These influences can be evaluated to variation of sensitivity in calibration test. In this paper, Calibration test were carried out for 3 types of bonding material i.e. stud, beewax and double side tape. Other factor was taken into consideration by 3-types for mounting block. All test was conducted by sinusoidal signal vibrator up to 4500Hz. In order to investigate influence for sensitivity from different input voltage in the calibrator, the same test was repeated. Test results were compared with standard accelerometer data. Relative sensitivities and phases were showed small difference in sensitivity for bonding materials with one block, but significant one for another block and different input voltage below 1000Hz.

• Proceedings of the KSME Conference
• /
• 2004.04a
• /
• pp.1047-1052
• /
• 2004

An efficient boundary-based technique is developed for addressing shape design sensitivity analysis in supercavitating flow problem. An analytical sensitivity formula in the form of a boundary integral is derived based on the continuum formulation for a general functional defined in potential flow problems. The formula, which is expressed in terms of the boundary solutions and shape variation vectors, can be conveniently used for gradient computation in a variety of shape design in potential flow problems. While the sensitivity can be calculated independent of the analysis means, such as the finite element method (FEM) or the boundary element method (BEM), the FEM is used for the analysis in this study because of its popularity and easy-touse features. The advantage of using a boundary-based method is that the shape variation vectors are needed only on the boundary, not over the whole domain. The boundary shape variation vectors are conveniently computed by using finite perturbations of the shape geometry instead of complex analytical differentiation of the geometry functions. The supercavitating flow problem is chosen to illustrate the efficiency of the proposed methodology. Implementation issues for and optimization procedure are addressed in this flow problem.

• East Asian Journal of Business Economics (EAJBE)
• /
• v.9 no.1
• /
• pp.71-81
• /
• 2021

Purpose - This study verified the mediating effect of volunteer activities in the influence of global citizenship consciousness on human rights sensitivity of university students majoring in business administration. Research design, data, and methodology - The survey was conducted on 172 4-year college students in G City, Korea. SPSS 23.0 program was used to perform frequency analysis, descriptive statistics analysis, correlation analysis, reliability analysis, and multiple regression analysis. In addition, path analysis was conducted to confirm the mediating effect of volunteer activities. Result - First, the "justice domain" had a statistically significant effect on human rights sensitivity. Second, the "cognitive domain" had a positive and significant effect on volunteer participation. Third, the volunteer participation of university students majoring in business administration had a positive and significant effect on human rights sensitivity. Fourth, in the relationship between global citizenship consciousness and human rights sensibility of university students, volunteer participation had a partial mediating effect. Conclusion - The period of college enrollment is an important time to establish life goals and strategies as adults. Therefore, if the university environment is built to grow into a healthy global citizen, it will help to cultivate global competence and the "sensitivity to human rights" that is more emphasized in the era of the 4th industrial revolution.

• Journal of Environmental Health Sciences
• /
• v.21 no.3
• /
• pp.96-101
• /
• 1995

A selective and highly sensitive spectrophotometric measurements have been developed for the determination of trace mercury(II) with thiosulfate and PAR. Hg(II)-thiosulfate complex was extracted into high molecular alkylamine such as quaternary ammonium salt and back extracted into 1 M-HCl solution. The Hg(II)-PAR complex has maximum absorbance at 499 nm and obeys Beer's law in the range of 0.04~1.0 $\mu g/mL$ of mercury(II). The molar absorptivity and Sandell's sensitivity are $6.27\times 10^4 L.mole^{-1} cm^{-1}$ and $3.2\times 10^{-3}g/cm^2$ respectively.

• Proceedings of the Korean Geotechical Society Conference
• /
• 1999.02a
• /
• pp.36-47
• /
• 1999

Terzaghi's theory of one-dimensional consolidation is restricted in its applicability to relatively thin layers and small incremental loading. Because it is assumed to infinitesimal strain and linear material function. For this reason, Gibson et al established a rigorous formulation for the one-dimensional nonlinear finite strain consolidation theory. There are some difficulties in the application of finite strain consolidation theory. The developed program consisted of several forms and modules. These forms and modules with graphic-user-interfaced format are used in analysis of consolidation practices. For the purpose of verification of developed program. the results of case study and prediction of developed program are compared. The results of comparison is fairly well with prediction and measured data. And with varying finite strain consolidation parameter, g(e) or λ(e), the sensitivity of predicted values were examined.