• Asian Pacific Journal of Cancer Prevention
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• v.13 no.10
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• pp.5069-5074
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• 2012

Objectives: The purpose of this study was to determine the relationship between methylation status of the Dact1 gene and MTHFR a1298c polymorphic forms in transitional cell carcinoma tissues in a Chinese population. Methods: Polymorphisms of folate metabolism enzyme gene MTHFR were assessed by restrictive fragment length polymorphism (RFLP) methods and PCR-based DNA methylation analysis was used to determine the CpG island methylation status of the Dact1 gene. Associations between the methylation status of the Dact1 gene and clinical characteristics, as well as MTHFR a1298c polymorphisms, were analyzed. Results: aberrant methylation of the Dact1 gene was found in 68.3% of cancer tissues and 12.4% of normal tissues,. The methylation rate of the Dact1 gene in cancer tissues was significantly higher in patients with lymph node metastasis than in those without lymph node metastasis (46.3% vs. 17.2%, P = 0.018). No association was found between aberrant DNA methylation and selected factors including sex, age, tobacco smoking, alcohol consumption and green tea consumption. After adjusting for potential confounding variables, variant allele of MTHFR a1298c was found to be associated with methylation of the Dact1 gene. Compared with wild type CC, the odds ratio was 4.33 (95% CI: 1.06-10.59) for AC and 4.95 (95% CI: 1.18-12.74) for AA. The N stage in TNM staging and the occurrence of lymph node metastasis were associated with an MTHFR 1298 AA+AC genotype (P<0.05). Conclusion: MTHFR 1298 AC and AA genotypes might help maintain a normal methylation status of the Dact1 gene, aberrant CpG island methylation of which is closely related to the genesis and progression of transitional cell carcinoma.

• BMB Reports
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• v.29 no.2
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• pp.127-132
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• 1996

Germinal centers (GCs) are formed in peripheral lymphoid tissues in response to protein antigens. In order to see if immunoglobulin isotype switching takes place in GC B-cells, we isolated GC B-cells (PNA positive cells) from mouse popliteal lymph nodes by a flow cytometer after the staining of lymph node cells with PNA-FITC and anti-B220-PE, and determined the expression of ${\gamma}1$ germline transcript and ${\gamma}1$ mRNA by RT-PCR. ${\gamma}1$ germline transcript and ${\gamma}1$ mRNA were amplified specifically in cDNAs from hybridoma expressing IgG1 or splenocytes stimulated LPS plus IL-4. Germinal center B-cells formed in popliteal lymph nodes of mice immunized with chicken ovalbumin were isolated 7 days after immunization. We sorted GC B-cells five times. Immunoglobulin ${\gamma}1$ germline transcripts were expressed in germinal center B-cells in three out of five sorts whereas two out of five sorts did not express ${\gamma}1$ germline transcripts in GC B-cells. The contents of GC B-cells ranged from 5 to 7% of total lymph node cells in most flow cytometric analyses but those of two sorted cells which did not express ${\gamma}1$ germline transcripts were out of normal range. These results imply that isotype switching of immunoglobulins may take place in GCs.

• Nutrition Research and Practice
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• v.1 no.2
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• pp.94-99
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• 2007

The immunostimulating activities of mucilage fraction from yam were investigated. The proliferation of BSA-primed lymph node cells was enhanced between 4.1- to 10.9-fold compare to control, when cultured with 1 to $25{\mu}g/mL$ of yam-mucilage fraction. It showed strong immunostimulating activity than ginseng extract and as remarkable as Bifidobacterium adolescentis M101-4 known as a positive immunostimulator. Mitogenicity to lymph node cells was fully induced by concanavalin A and lipopolysaccharide. The proliferation of splenocytes and Peyer's patch cells was enhanced between 5.0- to 14.1-fold and 2.4- to 6.4-fold, respectively, when cultured with 1 to $25{\mu}g/mL$ of yam-mucilage fraction. It enhanced the production of cytokines such as tumor necrosis $factor-{\alpha}$ and IL-6 in the culture of RAW 264.7 macrophage cells. In the culture of lipopolysaccharide-stimulated RAW 264.7 cells, production of cytokines was as similar as compared to controls. In unstimulated RAW 264.7 cells, both tumor necrosis $factor-{\alpha}$ and IL-6 production were enhanced between 15.6- to 60.1-fold and 2.3- to 9.1-fold, respectively. Mucilage fraction from yam is expected to be a safe immunopotentiator to maintain the host immunity and develop a physiologically functional food.

• Journal of Plant Biotechnology
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• v.37 no.1
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• pp.72-76
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• 2010

To develop transgenic cucumber tolerant to abiotic stress, a cotyledonary-node explants were co-cultivated with Agrobacterium tumefaciens (EHA101) carrying TPSP gene (pHC30-TPSP). After transfer to fresh medium every two week for eight weeks, putative transgenic plants were selected when shoots grown a length greater than 3 cm from the cotyledonary-node explants on selection medium supplemented with $5\;mgl^{-1}$ phospinotricin as selectable agent. The confirmation of transgenic cucumber was based on the Northern blot analysis. Thirty four shoots (5.2%) with resistance to phospinotricin were obtained from 660 explants inoculated. Of them, transformants were only confirmed from 11 plants (1.7%). Transgenic cucumber expressing TPSP gene was more synthesized at 3.8 times amounts of trehalose (0.014 mg g fresh $wt^{-1}$) than non-transformants (0.0037 mg g fresh $wt^{-1}$). However, all of transgenic plants showed abnormal morphology, including stunted growth (< height 15 cm), shrunken leaves, and sterility as compared with non-transgenic plants (> height 150 cm) under the same growth environment. These results lead us to speculate that the overproduction of trehalose was toxic for cucumber, even though that had known for rice as non-toxic.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.13 no.12
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• pp.5888-5903
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• 2019

The Relational ranking method applies authority-based ranking in relational dataset that can be modeled as graphs considering also their tuples' values. Authority directions from tuples that contain the given keywords and transfer to their corresponding neighboring nodes in accordance with their values and semantic connections. From our previous work, ObjectRank extends to ValueRank that also takes into account the value of tuples in authority transfer flows. In a maked difference from ObjectRank, which only considers authority flows through relationships, it is only valid in the bibliographic databases e.g. DBLP dataset, ValueRank facilitates the estimation of importance for any databases, e.g. trading databases, etc. A relational keyword search paradigm Object Summary (denote as OS) is proposed recently, given a set of keywords, a group of Object Summaries as its query result. An OS is a multilevel-tree data structure, in which node (namely the tuple with keywords) is OS's root node, and the surrounding nodes are the summary of all data on the graph. But, some of these trees have a very large in total number of tuples, size-l OSs are the OS snippets, have also been investigated using ValueRank.We evaluated the real bibliographical dataset and Microsoft business databases to verify of our proposed approach.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.29 no.12B
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• pp.1037-1041
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• 2004

In this paper we propose a new conceptual slave clock system in which remotely located clock is synchronized to the reference clock by intermediation of the satellite time, show a probability of adoption to real network by experiments. This new proposed method has lots of structural advantages over the existing methods because all of the node clocks can be maintained with the same hierarchical quality. The measurement results show that the accuracy of the experimental slave clock system can be kept within a few parts in 1012 and that the MTIE (Maximum Time Interval Error) meets the ITU-T Recommendation G.811 for the primary reference clock A prototype system having fully automatic operational functions has been realized, and it is expected to be commercially used as a node clock for synchronization in the digital communication network in the near future.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.13 no.6
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• pp.1-16
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• 2013

Smartphones and tablets including phone, calendar are the necessities of modern man. They are one of the MN(Mobile Node), each with wireless network capabilities. Necessities of modern human MNs are almost included cellular module available in LTE/3G and Wi-Fi module for high-speed Internet. Until now, MN mobility management is handled, but using network-based mobility management in this paper. Then, carriers can manage and maintain the network for low-cost. In addition, it was considered that use a lot of modern people with Wi-Fi and LTE/3G, and using Cross-Layer-Based handoff.

• Korean Journal of Veterinary Pathology
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• v.1 no.1
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• pp.7-12
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• 1997

Lethally irradited C3H/HeN mice were transplanted with syngeneic bone marrow. The B cell regeneration levels of spontaneous serum Ig, fecal igA and specific ig to diphtheria toxoid were determined at various time points. The number of B220+ cells reached normal range at 4 weeks after bone marrow transplantation(BMT) in spleen and lymph node. The B cell number of spleen returned to normal relatively soon than in the lymph node. Within 5 to 7 weeks after BMT, the transplanted mice contained nearly normal levels of spontaneous serum IgA, IgG2b and fecal IgA, but 2 fold lower levels of serum IgG2a, IgM and IgG3. Especially IgG3 levels were within low-normal range throughout the study. One to two weeks after immunization the predominant anti-diphtheria toxoid subtype was IgM. The levels of specific serum Ig were very low and after booster immunization at week 6, the short-lasting increase of Ig production was notd.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.7
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• pp.3187-3190
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• 2012

This study evaluated the relationship between pretreatment hemoglobin (Hb) and prognostic factors in Thai patients with endometrial cancer. Medical records of 228 patients who had undergone surgery between January 2005 and December 2007 were retrospectively reviewed. Associations between clinicopathological variables and pretreatment Hb levels were described using Pearson's chi square test or two-tailed Fisher's exact test. Survival analysis was performed with Kaplan-Meier estimates. Univariate and Cox-regression models were used to evaluate the prognostic impact of various factors, including Hb levels, in term of disease-free survival. The median duration of follow-up was 38.2 months. Eighty-nine patients (39%) had a preoperative Hb level of <12 g/dL, these having significantly higher rates of non-endometrioid histology, advanced FIGO stage, lymphovascular space invasion, cervical involvement, adnexal involvement, positive peritoneal cytology, and lymph node involvement than patients with Hb ${\geq}12$ g/dL. The 5-year disease-free and overall survival were significantly lower in patients with pretreatment Hb levels <12 g/dL compared with those with Hb ${\geq}12$ g/dL (79.3% vs. 89.2%, p=0.044 and 87.6% vs. 99.3%, p<0.001, respectively). In the multivariate analysis only histology, myometrial invasion, and lymphovascular invasion proved to be independent prognostic factors, whereas tumor grading, stage, cervical involvement, adnexal involvement, positive peritoneal cytology, lymph node involvement, and low Hb were not. In conclusion, presence of anemia before treatment may reflect poor prognostic factors in patients with endometrial cancer and low pretreatment hemoglobin level may have a prognostic impact on clinical outcome.

• Proceedings of the Korean Information Science Society Conference
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• 2006.06d
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• pp.97-99
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• 2006

무선 센서노드 사용한 데이터 계측 및 제어 기술은 특히 홈 네트워크 분야에 널리 적용되고 있다. 본 논문에서는 Ad-Hoc네트워크의 말단인 Base node와 인접한 노드들 사이에 조도 값을 실시간으로 전송하는 시스템을 구현하였다. 이는 OscilloscopeRF라고 하는 TinyOS에서 제공되는 프로그램이 사용하는 메시지 구조를 분석하여, 각 노드의 ID및 계측 데이터를 추출하였다. 이렇게 추출된 계측 데이터를 센서 네트워크의 Base node에서 데이터 통제 센터(DOC)로 효율적으로 전송하기 위해 TCP기반 네트워크 프로그래밍을 구현하였다. 실험 결과 센서 노드 수 및 샘플링 주기에 상관없이 안정적으로 계측 데이터 수신이 이루어짐을 확인하였다. 또한, IEEE802.11a/g 기반 무선 네트워크를 통해 계측 상황을 휴대용 단말기인 PDA에서 확인할 수 있도록 이를 구현하였다.