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Neutrophil oxidative burst as a diagnostic indicator of IgG-mediated anaphylaxis

  • Won, Dong Il;Kim, Sujeong;Lee, Eun Hee
    • BLOOD RESEARCH
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    • v.53 no.4
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    • pp.299-306
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    • 2018
  • Background IgG-mediated anaphylaxis occurs after infusion of certain monoclonal antibody-based therapeutics. New in vitro tests are urgently needed to diagnose such reactions. We investigated whether allergens trigger neutrophil oxidative burst (OB) and if neutrophil OB occurs due to allergen-specific IgG (sIgG). Methods Neutrophil OB was measured by dihydrorhodamine 123 flow cytometry using a leukocyte suspension spiked with a very small patch of the allergen crude extract, Dermatophagoides farinae (Der f). The mean fluorescence intensity ratio of stimulated to unstimulated samples was calculated as the neutrophil oxidative index (NOI). Results The Der f-specific NOI (Der f-sNOI) showed a time-dependent increase after Der f extract addition. At 15 min activation, higher Der f-sIgG levels were associated with lower Der f-sNOI values in 31 subjects (P<0.05). This inverse relationship occurs due to the initial blocking effect of free Der f-sIgG. Additionally, neutrophil OB was nearly absent (Der f-sNOI of -1) in two cases: a subject with undetectable Der f-sIgG levels and washed leukocyte suspensions deprived of Der f-sIgG. Conclusion Allergens can trigger neutrophil OB via preexisting allergen-sIgG. Neutrophil OB can be easily measured in a leukocyte suspension spiked with the allergen. This assay can be used to diagnose IgG-mediated anaphylaxis.

Effect of Maleic Ahydride Grafted PP on the Physical Properties of PP/Pulp Composites (PP/펄프 복합체의 물성에 미치는 말레인산무수물 그래프트 PP의 영향)

  • Lee, Jong Won;Kim, Won Gil;Kim, Youn Cheol
    • Polymer(Korea)
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    • v.38 no.5
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    • pp.566-572
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    • 2014
  • Maleic anhydride (MAH) grafted polypropylenes (PP) (MAH-g-PP) were prepared by changing MAH content and styrene monomer (SM)/MAH mole ratio with different type PP, using a twin screw extruder. The types of PP were isotatic PP (iPP), block PP (bPP), and random PP (rPP) and dicumyl peroxide (DCP) was used as an initiator. The graft degree of MAH was confirmed by the existence of carbonyl group (C=O) stretching peak at $3100cm^{-1}$ of FTIR spectrum. Thermal properties of MAH-g-PP and PP/MAH-g-PP/pulp composites were investigated by DSC and TGA. There was no district change in thermal properties of PP/MAH-g-PP/pulp composites. Based on tensile properties and SEM pictures for fractured surface of PP/MAH-g-PP/pulp composites, MAH-g-rPP was the best as the compatibilizer and optimum formulation was MAH content of 1.0 wt%, SM/MAH mole ratio of 1.0, and melt index (MI) of 25 g/10 min. The rheological properties of the composites were investigated by a dynamic rheometer. The complex viscosity, shear thinning effect, and water uptake incresed with pulp content.

Ratio of Torsion (ROT): An index for assessing the global induced torsion in plan irregular buildings

  • Stathi, Chrysanthi G.;Bakas, Nikolaos P.;Lagaros, Nikos D.;Papadrakakis, Manolis
    • Earthquakes and Structures
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    • v.9 no.1
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    • pp.145-171
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    • 2015
  • Due to earthquakes, many structures suffered extensive damages that were attributed to the torsional effect caused by mass, stiffness or strength eccentricity. Due to this type of asymmetry torsional moments are generated that are imposed by means of additional shear forces developed at the vertical resisting structural elements of the buildings. Although the torsional effect on the response of reinforced concrete buildings was the subject of extensive research over the last decades, a quantitative index measuring the amplification of the shear forces developed at the vertical resisting elements due to lateral-torsional coupling valid for both elastic and elastoplastic response states is still missing. In this study a reliable index capable of assessing the torsional effect is proposed. The performance of the proposed index is evaluated and its correlation with structural response quantities like displacements, interstorey drift, base torque, shear forces and upper diaphragm's rotation is presented. Torsionally stiff, mass eccentric single-story and multistory structures, subjected to bidirectional excitation, are considered and nonlinear dynamic analyses are performed using natural records selected for three hazard levels. It was found that the proposed index provides reliable prediction of the magnitude of torsional effect for all test examples considered.

Effects on the Laughter Score, Cortisol and Immunoglobulin of Laughter Therapy in Middle Aged Women (중년기 여성의 웃음치료가 웃음지수, Cortisol 및 면역글로불린에 미치는 효과)

  • Cha, Mi Youn;Hong, Hae-Sook
    • Journal of Korean Biological Nursing Science
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    • v.15 no.4
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    • pp.230-236
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    • 2013
  • Purpose: In this study, the effects of laughter therapy on a laughter index, cortisol and immunoglobulin of middle aged women were examined. Methods: A quasi-experimental nonequivalent control group pre/post-test design were used. The participants (n=54) included 27 in the experimental group and 27 in the control group. The data were collected from February through March of 2013. The experimental group participated in laughter therapy for 40 minutes per session, five times a week for a period of 2 weeks. The tool was used to measure pre/post-test laughter index, cortisol and immunoglobulin of middle aged women. Results: The results showed that laughter therapy was effective in increasing the laughter index (p<.001), and immunoglobulin IgA (p<.001), IgG (p<.001), and IgM (p=.010). Also, the stress hormone, cortisol of middle aged women decreased but non-significantly (p=.158). Conclusion: The result of the study indicates that laughter therapy may have an influence on nursing intervention and treatment effects to improve the laughter index and immunoglobulin of middle aged women.

Development of Hydroclimate Drought Index (HCDI) and Evaluation of Drought Prediction in South Korea (수문기상가뭄지수 (HCDI) 개발 및 가뭄 예측 효율성 평가)

  • Ryu, JaeHyun;Kim, JungJin;Lee, KyungDo
    • Journal of The Korean Society of Agricultural Engineers
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    • v.61 no.1
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    • pp.31-44
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    • 2019
  • The main objective of this research is to develop a hydroclimate drought index (HCDI) using the gridded climate data inputs in a Variable Infiltration Capacity (VIC) modeling platform. Typical drought indices, including, Standardized Precipitation Index (SPI), Standardized Precipitation Evapotranspiration Index (SPEI), and Self-calibrated Palmer Drought Severity Index (SC-PDSI) in South Korea are also used and compared. Inverse Distance Weighting (IDW) method is applied to create the gridded climate data from 56 ground weather stations using topographic information between weather stations and the respective grid cell ($12km{\times}12km$). R statistical software packages are used to visualize HCDI in Google Earth. Skill score (SS) are computed to evaluate the drought predictability based on water information derived from the observed reservoir storage and the ground weather stations. The study indicates that the proposed HCDI with the gridded climate data input is promising in the sense that it can help us to predict potential drought extents and to mitigate its impacts in a changing climate. The longer term drought prediction (e.g., 9 and 12 month) capability, in particular, shows higher SS so that it can be used for climate-driven future droughts.

A Study on the Planning of Nationwide Indexing Services for Korea (전국색인지간행협동체제 편성방안에 관한 연구)

  • Choi Sung Jin
    • Journal of the Korean Society for Library and Information Science
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    • v.12
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    • pp.39-86
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    • 1985
  • The main purpose of the present study is to survey the major iudexing bulletins of national nature in Korea, to define such problem areas as lacunae, duplicates and limitation in coverage in the indexing services currently available in Korea, and to make some suggestions for action for improving the existing indexing services in the light of general principles and the tradition and constraints unique to Korea. The major findings and conclusions reached at this study are summarised as follows: (A) A new indexing bulletin of general nature covering the entire field needs to be created in each of the following fields without an established indexing service available for the outcome of research and development activities in Korea. (1) Philosophy (2) Religion (3) Pure sciences (4) Art (5) Language (6) Literature (7) History (B) A new specialised indexing bulletin needs to be created in each of the following fields where indexing services are heavily utilised but no, or only partial, indexing service is available. (1) Social sciences (a) Statistics (b) Sociology (c) Folklore (d) Military science (2) Pure sciences (a) Mathematics (b) Physics (c) Chemistry (d) Astronomy (e) Geology (f) Mineralogy (g) Life sciences (h) Botany (i) Zoology (3) Applied sciences (a) Medicine (b) Agriculture (c) Civil engineering (d) Architectural engineering (e) Mechanical engineering (f) Electrical engineering (g) Chemical engineering (h) Domestic science (C) Publication of the indexing bulletins suggested in A and B above may be ideally carried on by a qualified and dependable learned society established in the respective fields and designated by the Minister of Education, and should be financially supported from the public fund under the provisions of Art. 27 of the Scientific Research Promotion Act of 1979. (D) The coverage and contents of the four indexing bulletins in the field of banking and financing published by the Library of the Bank of Korea are similar and considerably duplicated. It is, therefore, suggested that the four indexing bulletins are combined in one to form a more comprehensive and efficient bibliographical tool in the field and it is further developed into a general guide to the literature produced in the entire field of economics in Korea by gradually expanding its subject coverage. (E) For the similar reasons stated in D, the Index to the Articles on North Korea and the Catalogue of Theses on North Korea, both publisheds by the Ministry of Unification Library, are suggested to make into one. The Index to the Articles of the Selected North Korean Journals and the Index to the Articles of the North Korean Journals in Microfilm Housed in the Ministry of Unification Library, both published by the same Library, are also suggested to be combined in one. (F) The contents of the Catalogue of the Reports Submitted by Government Officials Who Have Travelled Abroad, published by the National Archives are included in the Index to the Information Materials Related to Government Administration, published by the National Archives. The publication of the former is hardly justified. (G) The contents of the Index to Legal Literature published by the Seoul National University Libraries and those of the Law Section of the Index to Scholastic Works published by the National Central Library are nearly identical. One of the two indexes should cease to be published. (H) Though five indexes are being published in the field of political science and four in the field of public administration, their subject coverage is limited. Naturally, these indexes are little usable to many other researchers in the two fields. A comprehensive index covering all the specialised areas in each field needs to be developed on one or all the existing indexes. (I) It is suggested that the Catalogue of the Scholastic Works on Curricula published by the National Central Library expands its subject coverage to become a more usable and effective index to all the researchers in the field of education. (J) The bimonthly Index to Periodical Articles and the specialised index by subject series published by the National Assembly Library, and the Index to Scholastic Works published by the National Central Library are expected to increase their coverage and frequency of publication to be used more effectively and more efficiently by all users in all fields till the indexing bulletins suggested in this study will fully be available in Korea.

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Fish Fauna and Guild Compositions in Geum River Watershed

  • Lee, Eui-Haeng;Kim, Hyun-Mac;Lee, Jae-Kwan;Byeon, Myeong-Seop;An, Kwang-Guk
    • Korean Journal of Ecology and Environment
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    • v.41 no.4
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    • pp.490-498
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    • 2008
  • This study was to analyze fish composition and ecological indicator characteristics from eight sampling sites of Geum River, October 2007. Total number of family and species sampled were 9 and 40. The most dominant family was Cyprinidae (27 species, 85%), and then followed by Cobitidae, Odomtobutidae, and Gobiidae. Constancy values of Zacco platypus and Zacco temminckii were 1.00 and 0.30, respectively, and the relative abundance of Acheilognathus koreensis and Pseudogobio esocinus were greater than 5% of the total. The number of Korean endemic species sampled in this Geum River study was 7 family 19 species, which is 47.5% of total 40 species, and endangered species of Pseudopungtungia nigra and Gobiobotia brevibarba were only 0.5% of the total and these species were only distributed within the upstream regions. Exotic species, Micropterus salmoides, which is known as large-mouth bass, were observed in two sites of G3 and G7. Analytical results of fish community showed that community dominance index was 0.19, which is low, and the species evenness index (0.74), diversity index (2.03), and richness index (3.00) appeared high. These results indicate that structure of fish community is stable in the Geum River. According to various guilds analysis, the relative abundance of tolerant and omnivores at all sites were 40% and 47% of the total, respectively. This monitoring data may contribute changes of fish fauna and compositions in relation to habitat modifications and chemical water quality degradations in the future.

Species Composition and Diversity of Fisheries Resources, Nekton, off the Coast of Hwasun, Southern part of Cheju Island (제주도 남부 화순연안 수산자원 유영생물의 종 조성과 다양도)

  • Go, You-Bong;Shin, Heau-Sub
    • Korean Journal of Ichthyology
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    • v.2 no.1
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    • pp.36-46
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    • 1990
  • Samples were obtained with a set net during 7 months between March and October in 1987 off the coast of Hwasun, southern part of Cheju Island. Most of organisms, representing 36 species, were less than 20cm(96%) in length, and 30g(74%) in weight. The four most abundant species were jack mackerel, Trachurus japonicus ; squid, Todarodes pacificus ; mackerel, Scomber japonicus ; and Apogon semilineatus, which comprised about 89% of the total individuals and about 70% of the total weight. Diversity index, the number of species and information index for individual were high in early spring, even though biomass was not abundant. On the other hand, diversity index and information index for individual were relatively high in August in spite of low occurrence of the species number. A cluster analysis of 24 species which appeared more than twice during the 7 samplings were illustrated from the similarity matrix. All of the 24species were grouped at the 0.14 similarity level. They were divided into four groups at 0.50 level.

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Effect of Cold Brine Immersion Time on Sliced Raw Fish Texture (생선회의 육질향상에 미치는 저온 브라인 침지시간의 영향)

  • SHIM, Kil-Bo;LEE, Gi-Bong;KIM, Tae-Jin;LEE, Keun-Woo;KIM, Geon-Bae;LEE, In-Soo;CHO, Young-Je
    • Journal of Fisheries and Marine Sciences Education
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    • v.15 no.1
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    • pp.57-66
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    • 2003
  • The purpose of the present study was to investigate physicochemical changes in olive flounder and korean rockfish muscle subjected to the cold brine, and to find the optimal immersion condition. Effects of various immersion conditions in a sliced raw fish quality were investigated in the rigor index, breaking strength, ATP related compounds, and lactate accumulation in the muscle. The onset of rigor-mortis was accelerated by the increase of immersion time. Also, the time reaching full rigor was shortened remarkably too. However, the rigor index of samples immersed in the cold brine decreased more than that of samples killed instantly. Rigor index was the highest in samples killed instantly, followed by 2.5 min, 5min, 7.5 min, 10 min and 15 min in order. Breaking strength of all samples immersed in the cold brine decreased significantly after reaching the maximum values. The content of ATP related compounds in olive flounder, korean rockfish were $12.0{\mu}mole/g$ and $8.3{\mu}mole/g$, respectively. However, ATP decreased in the samples immersed in the cold brine. The content of ATP was lower, at the optimal condition and then it was increased. Also, lactates increased, at the optimal condition and then it decreased, in contrast with the ATP. The results suggested that the optimal immersion condition of olive flounder and korean rockfish was at -${12.5}^{\circ}C$ for 5min.

Seasonal Variation in Macroalgal Community Structure around the Byeonsan Peninsula, Korea (변산반도 해조류 군집구조의 계절적 변동)

  • Han, Su Jin;Lee, Ji Hee;Jeon, Da Vine;Oh, Ji Chul;Kim, Bo Yeon;Park, Seo Kyoung;Choi, Han Gil;Nam, Ki Wan
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.47 no.3
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    • pp.274-282
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    • 2014
  • We examined seasonal variation in the structure of marine macroalgal communities at five sites around the Byeonsan Peninsula, Korea, from January to October 2011. Sixty eight species were identified, including 11 green, 17 brown and 40 red algae. Species richness was highest at Gyeokpo (51 species) followed by Sambal (47), Gosapo (34), Byeonsan (33), and Habgoo (18). Seaweed biomass ranged from 12.98 to 145.33 g dry $wt/m^2$, with maximum and minimum biomasses at Gyeokpo and Habgoo, respectively. Sargassum thunbergii was the representative alga species occupying 47.89% of the total biomass. The dominant seaweeds were S. thunbergii, Corallina pilulifera, Ulva spp, Gracilaria vermiculophylla, and Carpopeltis affinis. No distinctive vertical distribution of seaweeds was found because S. thunbergii, Ulva spp, and G. vermiculophylla were all distributed from high to low shore. Community indices were as follows: dominance index (DI), 0.44-0.61; richness index (R), 6.27-10.35; evenness index (J'), 0.38-0.59; and diversity index (H'), 1.48-1.71. Gyeokpo had the most biomass and highest species richness, and the lowest percentage of Ulva species. However, species richness declined at Gyeokpo, from 78 to 51 species within 10 years, implying that eutrophication and sedimentation from Mankyeong and Dongjin rivers have reduced the stability of seaweed communities.