This study was designed to evaluate the characteristics, land use and genesis of well adapted paddy soils. They were mostly classified as the Haplaquepts in the U.S.D.A soil taxonomy and as the Grey soils in the Japanese soil classification system. The proportion of these soils in the total acreage of paddy lands was thirty three percents. The fifty four percents in average of these soils were distributed on the local valley and fans on gentle slopes developed from granite, granite gneiss and shale parent materials. The rests were on the fluvio-marine deposits and alluvial deposits. The soils were characterized with prominant development of gleized horizons and clayey or fine loamy textured category. The available soil depth and the ground water level were relatively deep. The base saturation percent were high with weak acidic pH. The potential productivity of these soils was high.
In this study, we investigated antioxidant capacity and determined the phenolic and flavonoid contents using each of various solvent conditions from fermented Rhus verniciflua stem bark (F-RVS). Each extracts displayed markedly similar content of extraction yield. However, M80 extract showed a significantly higher antioxidant activity in comparison to other extract investigated. M80 exhibited strong DPPH radical scavenging activity with $RC_{50}$ value of $10.5{\pm}1.4{\mu}g/m{\ell}$, reducing power value 1.04 Abs (concentration of 1 mg/$m{\ell}$), and anti-lipid peroxidation activity value of 94.6% (concentration of 10 mg/$m{\ell}$). M80 extract showed the high content of total phenolic (319.7 mg GAE/$m{\ell}$ extract) and total flavonoid (111.6 mg QE/$m{\ell}$ extract). Phenolic and flavonoid compounds showed significant relationship in DPPH radical scavenging ($R^2$=0.911 and 0.912, each extract) and reducing power ($R^2$=0.903 and 0.837, each extract) from fermented R. verniciflua stem bark. However, antilipid peroxidation activity ($R^2$=0.589 and 0.441, each extract) was not significant like DPPH radical scavenging and reducing power. Therefor the result indicated that the potential antioxidant activities and functional values were ovserved significantly at M80 solvent condition from the fermented R. verniciflua stem bark.
The experiment was conducted at Gyeongbuk Provincial Rural Development Administration in 1996 to obtain basic information on weed ecology and effective weed control in direct-seeded rice fields under different seeding types and seeding times. A large number of upland weeds such as Echinochloa crus-galli, Digitaria sanguinalis, Capsella bursa-pastoris and Stetaria viridis etc occurred at early growth stage, while Eleocharis kuroguwai, Aneilema keisak and Cyperus serotinus dominated at late stage in dry seeded rice field. In wet seeded rice field, the dominance of E. crus-galli was lower and the occurrence of M. vaginalis and A. keisak were higher than in dry seeded field. Amount of weed occurrence in dry seeded field was 1.8 to 2.4 times greater than in wet seeded field and it increased with delaying seeding time. Increasing rate of weed occurrence through whole life cycle was the highest at 20 days after seeding (DAS) to 40 DAS, regardless of seeding types and seeding times. Simpson index in wet seeded field was higher than in dry seeded field and it gradually increased as growing of dominant species. For F-value test, it was recognized as statistical significant in seeding types, seeding times and interaction of two factors.
This study was carried out to evaluate the effects of dietary supplementation of different sources of chromium on growth performance, blood profile and carcass trait in growing-finishing pigs. A total of 200 growing pigs (Landrace${\times}$Yorkshire)${\times}$Duroc, average initial weight 8.5 kg) were allotted to 5 treatments with 4 replicates per treatment and 10 pigs per replicate. Five treatments were designated as follows according to the source of chromium. i) Control (No chromium): corn-soybean meal based basal diet, ii) $CrCl_{3}$: control diet+200 ppb Cr as $CrCl_{3}$, iii) CrPic: control diet+200 ppb Cr as Cr picolinate, iv) CrMet-1: control diet+100 ppb Cr as Cr methionine, and v) CrMet-2: control diet+200 ppb Cr as Cr methionine. After the feeding trial, three pigs per replicate (12 pigs per treatment) were slaughtered for the evaluation of carcass traits. Average daily gain (ADG), average daily feed intake (ADFI), and feed: gain ratio (F/G) were not different (p>0.05) among dietary Cr sources. However, whole-period ADG of pigs fed CrPic, CrMet-1 and CrMet-2 diets was higher (p<0.05) than for the control diet. Nutrient digestibility was not different (p>0.05) among dietary Cr sources, but the nutrient digestibility of pigs fed CrPic, CrMet-1 and CrMet-2 diets was higher (p<0.05) than for the control diet. BUN level decreased with more magnitude (p<0.05) in pigs fed Cr during the 20 to 50 kg period. Although both serum cholesterol and triglyceride were different (p<0.05) among treatments, there was no consistent response that could be related to the dietary Cr sources regardless of growth phase. However, the overall data suggested that serum cholesterol level increased as BW of pigs increased. Blood total protein (TP) increased (p<0.05) in pigs fed Cr only during the 90-110 kg phase, and blood creatinine (Creat) level was higher in $CrCl_{3}$ and CrPic treatments than in the control only during the 90-110 kg phase. Backfat thickness was thinner (p<0.05) in pigs fed CrMet-2 than in the control treatment. Therefore, lean percentage was higher (p<0.05) in CrMet-2 than in control pigs. However, dressing percentage and Longissimus muscle area (LMA) were not different (p>0.05) among treatments. In conclusion, dietary supplementation of 200 ppb Cr, via either CrPic or CrMet, improved pig growth performance and nutrient digestibility. Moreover, dietary CrMet supplementation for the growing-finishing pig is evidently remarkable for improving both lean percentage of the carcass and backfat thickness.
Objective: An experiment was conducted to evaluate dietary supplemental trace mineral source and deletion on mineral content in tissues. Methods: Weanling crossbred pigs (n = 144; 72 barrows and 72 gilts; body weight [BW] = $7.4{\pm}1.05kg$) were used. A basal diet was prepared, and trace mineral premix containing either inorganic (ITM) or organic (OTM) trace minerals (Cu, Fe, Mn, and Zn) was added to the basal diet. Pigs were blocked by sex and BW and randomly allotted to 24 pens for a total of 6 pigs per pen, and fed a diet containing either ITM or OTM supplemented at the 1998 NRC requirement estimates for each of 5 BW phases (Phase I to V) from 7 to 120 kg. The trace mineral supplementation was deleted for 6, 4, 2, and 0 wk of Phase V; regarding nutrient adequacy during this phase, the indigenous dietary Fe and Mn was sufficient, Cu was marginal and Zn was deficient. Results: At the end of Phase IV, Mn content (mg/kg on the dry matter basis) was greater (p<0.05) in heart (0.77 vs 0.68), kidney (6.32 vs 5.87), liver (9.46 vs 8.30), and longissimus dorsi (LD; 0.30 vs 0.23) of pigs fed OTM. The pigs fed OTM were greater (p<0.05) in LD Cu (2.12 vs 1.89) and Fe (21.75 vs 19.40) and metacarpal bone Zn (141.86 vs 130.05). At the end of Phase V, increased length of deletion period (from 0 to 6 wk) resulted in a decrease (linear, p<0.01) in liver Zn (196.5 to 121.8), metacarpal bone Zn (146.6 to 86.2) and an increase (linear, p<0.01) in heart Mn (0.70 to 1.08), liver Mn (7.74 to 12.96), and kidney Mn (5.58 to 7.56). The only mineral source by deletion period interaction (p<0.05) was observed in LD Zn. Conclusion: The results demonstrated differential effects of mineral deletion on tissue mineral content depending on both mineral assessed and source of the mineral.
Koreanishche Zeitschrift fur Deutsche Sprachwissenschaft
/
v.4
/
pp.129-153
/
2001
In der vorliegenden Arbeit wird das $Verh\"{a}ltnis$ zwischen den syntaktischen und semantischen Typen von $Konditionals\"{a}tzen$ und deren kommunikativen Funktionen untersucht. Nach der $g\"{a}ngigen$ Auffassung haben Konditionalsatze folgende syntaktische und semantische Eingenschaften: 1. Der Konditionalsatz besetzt das Vorfeld des Hauptsatzes. 2. Im Hauptsatz kann ein Korrelat wie dann eingefugt werden. 3. Mit dem Konditionalsatz wird eine Bedingung oder eine Voraussetzung $f\"{u}r$ den im Hauptsatz $ausgedr\"{u}ckten$ Sachverhalt genannt. Aber im Korpus finden sich auch $Konditionals\"{a}tze$ wie folgende, die keine dieser Eigenschaften haben: (1) Wenn du es noch nicht $wu{\ss}test$, Karl ist wieder im Lande. (2) Wenn ich mal kurz einwerfen darf, er muss sich operieren lassen. Ausgehend van der Annahme, dass die syntaktische und semantische Struktur des $Konditionalsatzgef\"{u}ges$ mit der kommunikativen Funktion des Konditionalsatzes $zusammenh\"{a}ngt,\;wird\;zun\"{a}cht$ versucht, die Typen der $Konditionals\"{a}tze$ aus syntaktischer und semantischer Hinsicht herauszustellen. Danach wird jeder Typ auf die kommunikative Funktion hin $\"{u}berpr\"{u}ft$. Daraus haben folgende Ergebnisse resultiert: 1. Hinsichtlich der syntaktischen Relation zwischen dem Konditionalsatz und dessen Hauptsatz wird zwischen zwei Typen von $Konditionalsatzgef\"{u}gen$ unterschieden: $n\"{a}mlich$, die verbundene und die nicht-verbundene Konstruktion. 2. Hinsichtlich der semantischen Relation zwischen dem Konditionalsatz und dessen Hauptsatz wird zwischen zwei Typen von $Konditionalsatzgef\"{u}gen$ unterschieden: $n\"{a}mlich$, die integrierte und die nicht-integrierte Konstruktion. 3. In der Regel weisen die $Konditionalsatzgef\"{u}ge$ mit der integrierten Konstruktion syntaktisch eine verbundene Konstruktion auf, wahrend die $Konditionalsatzgef\"{u}ge$ mit der nicht-integrierten Konstruktion eine nicht-verbundene Konstruktion haben. 4. In Bezug auf die $illokution\"{a}re\;Selbst\"{a}ndigkeit$ wird zwischen zwei Typen van $Konditionals\"{a}tzen$ unterschieden: $n\"{a{mlich,\;Konditionals\"{a}tze$ mit einer $selbst\"{a}ndigen\;illokution\"{a}ren$ Rolle und $Konditionals\"{a}tze$ ohne eine solche. 5. Die $Konditionals\"{a}tze$ in der verbundenen und integrierten Konstruktion haben keine $selbst\"{a}ndige\;illokution\"{a}re\;Rolle,\;w\"{a}hrend\;die\;Konditionals\"{a}tze$ in der nicht-verbundenen und nicht-integrierten Konstruktion eine $selbst\"{a}ndige\;illokution\"{a}re$ Rolle haben. 6. In Bezug auf die kommunikative Funktion der $Konditionals\"{a}tze$ mit einer $selbst\"{a}ndigen\;illokution\"{a}ren$ Roll wird zwischen drei Typen von $Konditional\"{a}tzen$ unterschieden: Relevanz-Konditionale, metakommunikative Konditionale und diskursstrukturierende Konditionale.
Lee, C.K.;Moore, K.;Scales, N.;Westhusin, M.;Newton, G.;Im, K.S.;Piedrahita, J.A.
Asian-Australasian Journal of Animal Sciences
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v.13
no.5
/
pp.587-594
/
2000
At present embryonic stem (ES) cells with confirmed pluripotential properties are only available in the mouse. Recently, we were able to isolate, culture and genetically transform primordial germ cell (PGC)-derived cells from pig embryos and demonstrate their ability to contribute to chimera development in the pig. In order to determine whether the system we developed could be used to isolate embryonic germ (EG) cells from other mammalian species, we placed isolated PGCs from cattle, goats, rabbits and rats in culture. Briefly, PGCs were isolated from fetuses of cow (day 30-50), goat (day 25), rabbit (day 15-18) and rat (day 11-12), and plated on STO feeder cells in Dulbecco's modified Eagle's medium (DMEM): Ham's F10 medium (1:1) supplemented with 0.01 mM nonessential amino acids, 2 mM L-glutamine, 0.1 mM $\beta$ - mercaptoethnol, soluble recombinant human stem cell factor (SCF; 40ng/ml), human basic fibroblast growth factor (bFGF; 20ng/ml) and human leukemia inhibitory factor (LIF; 20ng/ml). For maintenance of the cells, colonies were passed to fresh feeders every 7-10 days. In all species tested, we were able to obtain and maintain colonies with ES-like morphology. Their developmental potential was tested by alkaline phosphatase (AP) staining and in vitro differentiation assay. For genetic transformation, cells were electroporated with a construct containing the green fluorescent protein (GFP) under the control of the cytomegalovirus (CMV) promoter. GFP-expressing colonies were detected in cattle, rabbits and rats. These results suggest that PGC-derived cells from cattle, goats, rabbits and rats can be isolated, cultured, and genetically transformed, and provide the basis for analyzing their developmental potential and their possible use for the precise genetic modification of these species.
Pang, X.S.;Wang, Z.Y.;Zhu, T.G.;Yin, D.Z.;Zhang, Y.L.;Meng, L.;Wang, F.
Asian-Australasian Journal of Animal Sciences
/
v.23
no.2
/
pp.188-196
/
2010
The objective of this study was to characterize the litter sizes in Huanghuai goats with high prolificacy (HP, five or more kids born per litter on at least one occasion), and to compare their peripheral blood concentrations of progesterone and estradiol with those of goats with poor prolificacy (PP, up to three kids born per litter on any occasion). The circulating concentrations of progesterone and estradiol were measured by radioimmunoassay from daily blood samples taken during natural estrus cycles and at 1-5 days after ovariectomy. Estrus was synchronized using two doses of a prostaglandin analog. Litter size for the HP goats increased up to a parity of five and decreased thereafter. The percentage of goats with litter sizes of $\geq$4 from parities 3 to 6 ranged from 44.5% to 58.3%. Although small differences in litter size were obtained for goats of parities three, four and six relative to five, parity five does had the highest mean litter size. Progesterone concentrations began to rise earlier and were higher in the HP than in the PP goats on most days of the luteal phase, but not during the follicular phase of the cycle or after ovariectomy. There was a significant difference between the two groups (p<0.05) in the magnitude of the progesterone plateau. Mean estradiol concentrations in the HP group remained significantly higher than in the PP group (p<0.05) during the estrus cycle. There were two estradiol peaks in the HP goats during the early luteal phase, but only one in the PP group. Measurements of individual corpora lutea (CL) in vitro showed that there was a greater prevalence of small CL (<6 mm in diameter) in the HP group than in the PP group (p<0.05). After ovariectomy, the estradiol level on day 1 was significantly higher than at the nadir during the estrus cycle in both the HP (p<0.01) and PP (p<0.05) goats, while levels decreased by 12.3% and 26.2% respectively compared with the mid-luteal period in HP and PP goats (p<0.05). The overall mean estradiol concentrations in HP goats were lower than in the PP group, but no significant differences were found between groups at 1-5 days after ovariectomy.
Kim, E.T.;Min, K.S.;Kim, C.H.;Moon, Y.H.;Kim, S.C.;Lee, Sung-Sill
Asian-Australasian Journal of Animal Sciences
/
v.26
no.4
/
pp.517-522
/
2013
The effect on methanogens attached to the surface of rumen ciliate protozoa by the addition of plant extracts (pine needles and ginkgo leaves) was studied with particular reference to their effectiveness for decreasing methane emission. The plant extracts (pine needles and ginkgo leaves) were added to an in vitro fermentation incubated with rumen fluid. The microbial population including bacteria, ciliated-associated methanogen, four different groups of methanogens and Fibrobacter succinogenes were quantified by using the real-time PCR. Gas profiles including methane, carbon dioxide and hydrogen, and runinal fermentation characteristics were observed in vitro. The methane emission from samples with an addition of individual juices from pine needles, ginkgo leaves and 70% ethanol extract from ginko leaves was significantly lower (p<0.05, 27.1, 28.1 and 28.1 vs 34.0 ml/g DM) than that of the control, respectively. Total VFAs in samples with an addition of any of the plant extracts were significantly lower than that of the control (p<0.05) as well. The order Methanococcales and the order Methanosarcinales were not detected by using PCR in any incubated mixtures. The ciliate-associated methanogens population decreased from 25% to 49% in the plant extacts as compared to control. We speculate that the supplementation of juice from pine needles and ginkgo leaves extract (70% ethanol extract) decreased the protozoa population resulting in a reduction of methane emission in the rumen and thus inhibiting methanogenesis. The order Methanobacteriales community was affected by addition of all plant extracts and decreased to less than the control, while the order Methanomicrobiales population showed an increase to more than that of the control. The F. succinogenes, the major fibrolytic microorganism, population in all added plant extracts was increased to greater than that of the control. In conclusion, pine needles and ginkgo leaves extracts appear to have properties that decrease methanogenesis by inhibiting protozoa species and may have a potential for use as additives for ruminants.
Two feeding trials were conducted to study the effects of different chelated copper and zinc compounds on the performance and fecal excretions of weanling pigs. In Exp. 1, 150 weanling pigs ($L{\times}Y{\times}D$, $12.30{\pm}2.07kg$) were randomly assigned to five dietary treatments: 170 ppm Cu from $CuSO_4$, 85 ppm Cu from Cu amino-chelate (CAC), 170 ppm Cu from CAC, 85 ppm Cu from Cu-Lysine (CL), and 170 ppm Cu from CL. In Exp. 2, 150 weanling pigs ($L{\times}Y{\times}D$, $12.52{\pm}1.80kg$) were randomly assigned to five dietary treatments: 120 ppm Zn from $ZnSO_4$, 60 ppm Zn from Zn-amino-chelate (ZAC), 120 ppm Zn from ZAC, 60 ppm Zn from Zn-Methionine (ZM), and 120 ppm Zn from ZM. In both experiments, pigs were randomly distributed to the treatments following a randomized complete block design on the basis of body weight as the blocking variable. Each experiment was conducted for 28 days. Blood and fecal samples were collected to determine mineral contents as affected by the dietary treatments. There was no difference (p>0.05) in ADG and ADFI among treatments, but F/G was improved (p<0.05) in pigs fed diet with 170 ppm CAC than 85 ppm CL but not different (p>0.05) to the control (170 ppm $CuSO_4$). Regardless of copper source, concentration of Cu in serum and feces were higher in pigs fed diet with 170 ppm Cu than pigs fed diet with 85 ppm Cu (Exp 1). In Exp 2 the ADG was higher (p<0.05) in pigs fed diet with 120 ppm ZM than in pigs fed diets with 120 ppm $ZnSO_4$ and 60 ppm ZAC and ZM. The serum zinc concentration was generally higher (p<0.05) in pigs fed diet with organic source than the control group ($ZnSO_4$). Also, there was a trend towards a decrease in fecal excretions of zinc when dietary zinc level was low. The efficacy of the two chelated copper and zinc sources is similar in terms of growth performance. The fecal excretions for Cu and Zn could be reduced in pigs fed low level of these minerals using organic sources.
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