• Title/Summary/Keyword: G-민감도

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Immunological Characterization of Antigen from Cysticercus and Sparganum, and their Application to the Development of Immunodiagnostic System 2. Immunological Characteriaation of Crude Antigenic Components from Sparganum (Cysticercus와 Sparganum에서 추출한 조항원의 면역학적 특성과 그의 면연진단에 응용 2. Sparganum의 조항원성 분의 면역학적 특성)

  • ;James Yang
    • The Korean Journal of Zoology
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    • v.32 no.4
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    • pp.429-440
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    • 1989
  • Spirometru erinocei의 유충인 sparganum에서 추출한 조항원 단백질을 항원으로 하여 sparganosis, cysticercosis, hydatidosis환자의 IgG 항체와 정상인의 IgG 항체를 혈청반응시켜 ELISA와 EITB에 의해 교차반응을 일으키는 비 특이항원 성분과 종특이항원 성분을 추구하였다. 1. sparganum에서 0.01 M PBS (PH 7.4)로 추출한 조항원 단백질을 SDS-PAGE로 전개하여 290 Kd에서 23 Kd범위의 분자량을 가진 25개의 단백질이 분획되었다. 2. sparganum의 조항원을 항원으로하여 ELISA로 sparganosis, cysticercosis, hydatidosis 환자의 190 항체와의 혈청 반응치는 sparganosis환자에게서는 0.44 $\pm$ 0.07에서 1.90 $\pm$ 0.03으로 negative control normal sera의 0.D (0.15 $\pm$ 0.03)를 기준으로 하였을 때 모두 양성이며 민감도(sensitivity)가 100 %이었으며 cysticercosis, hydatidosis환자혈청에서 양성반응이 나타났으며 교차반응도 있었다. 3. EITB에서는 spargancsis환자의 IgG항체에 의해 16개의 항원성분이 인지되었으며 이 중 6개의 항원성분이 정상인의 혈청에서도 인지되어 교차반응을 일으키는 항원성분이었으며 cysticercosis환자혈청에서 인지된 4개의 항원성분 중 2개의 항원성분이 sparganosis 환자혈청에서 인지된 것과 같았으며 hydatidosis환자의 190항체에 의해 인지된 19개의 항원성분 중 12개의 항원성분이 sparganosis환자혈청에서 인지된 항원 성분과 같았다. 4. 290 Kd, 200 Kd, 28 Kd의 항원성분은 sparganosis환자의 196항체에서만 인지되었고 228 Kd, 152 Kd, 66 Kd항원성분은 hydatidosis환자의 19G항체에서만 인지되었으며 66 Kd항원성분은 sparganosis, cysticercosis, hydatidosis, 정상인의 혈청에서 모두 인지되었다. We studied the serological reaction between the specific and nonspecific antigenic components from metacestode (plerocercoid) of spiromeko erimacei and IgG antibodies in sparganosis, cysticercosis. hydatidosis patients and normal human sera by ELISA and EITB. We prepared the crude extracts of sparganlim from snake, Matrix tigrina laterolis and used as antigenic components. By SDS-PAGE, we detected a total 25 peptide bands (fractions) with 290 Kd to 23 Kd molecular weight, and 8 bands of these detected bands developed strongly by silver stain. In serological test, ELISA, we recognized the cross-reaction of antigenic components reacting with IgG antibodies in heterogenous sera, cysticercosis and hydatidosis patients sera. The crude antigenic components of sparganum showed the high sensitivity in sparganosis, hydatidosis patients sera, but showed lower sensitivity in cysticercosis patients sera than the sparasanosis, hydatidosis patients sera. Sixteen antigenic components of these 25 separated bands were recognized by antibodies In sparsanosis patients sera,8 antigenic components in normal human sera, 4 antigenic components in cysticercosis patients sera and 19 antigenic compoenents in hydatidosis patients sera. The crude antigenic compnenets with 290 Kd, 200 Kd, 125 Kd and 28 Kd molecular weight was only recognized in sparganosis patients sera, but 64 Kd antigenic component was nonspecific antigenic components which were also cross-reacted with sparganosis, hydatidosis, cysticercosis patients sera and normal human sera.

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Quantitative Risk Assessment of Listeria monocytogenes Foodborne Illness Caused by Consumption of Cheese (위해평가를 통한 치즈에서의 Listeria monocytogenes 식중독 발생 가능성 분석)

  • Ha, Jimyeong;Lee, Jeeyeon
    • Journal of Food Hygiene and Safety
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    • v.35 no.6
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    • pp.552-560
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    • 2020
  • Listeria monocytogenes is a highly pathogenic gram-positive bacterium that is easily isolated from cheese, meat, processed meat products, and smoked salmon. A zero-tolerance (n=5, c=0, m=0/25 g) criteria has been applied for L. monocytogenes in cheese meaning that L. monocytogenes must not be detected in any 25 g of samples. However, there was a lack of scientific information behind this criteria. Therefore, in this study, we conducted a risk assessment based on literature reviews to provide scientific information supporting the baseline and to raise public awareness of L. monocytogenes foodborne illness. Quantitative risk assessment of L. monocytogenes for cheese was conducted using the following steps: exposure assessment, hazard characterization, and risk characterization. As a result, the initial contamination level of L. monocytogenes was -4.0 Log CFU/g in cheese. The consumption frequency of cheese was 11.8%, and the appropriate probability distribution for amount of cheese consumed was a Lognormal distribution with an average of 32.5 g. In conclusion, the mean of probabilities of foodborne illness caused by the consumption of cheese was 5.09×10-7 in the healthy population and 4.32×10-6 in the susceptible population. Consumption frequency has the biggest effect on the probability of foodborne illness, but storage and transportation times have also been found to affect the probability of foodborne illness; thus, management of the distribution environment should be considered important. Through this risk assessment, scientific data to support the criteria for L. monocytogenes in cheese could be obtained. In addition, we recommend that further risk assessment studies of L. monocytogenes in various foods be conducted in the future.

Evaluation of Maximum Absorption and Removal Efficiency of Commercial Active Charcoal to the Typical Odorous Gases (시판 활성탄의 악취 가스에 대한 최대 흡수량과 제거효율 측정)

  • 곽민규;문영준;양성봉
    • Proceedings of the Korea Air Pollution Research Association Conference
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    • 1999.10a
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    • pp.397-398
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    • 1999
  • 활성탄은 1,000m$^2$/g 이상의 큰 표면적을 가지며, 이것을 활용하여 공업적인 용도로 예부터 탈취제와 같은 흡착제로서 이용되고 있다. 최근에는 생활 수준의 향상으로 인해 악취에 대해 민감해져서 악취 제거제로서 활성탄의 사용량은 갈수록 늘어나고 있는 추세이다. 특히 우리나라의 경우 공업단지 주변에서의 악취에 대한 민원이 급증함으로써 여러 악취발생업체에서 악취 제거장치로서 활성탄을 이용하는 곳이 많아지고 있다.(중략)

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Business Opportunity Analysis of the power amplifier module (전력 증폭기 모듈의 사업기회 분석)

  • Kwon, young-il
    • Proceedings of the Korea Contents Association Conference
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    • 2016.05a
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    • pp.327-328
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    • 2016
  • 전력 증폭기 모듈 패키지 산업은 전방산업의 경기에 매우 큰 영향을 받고 종속성이 높은 산업이므로 수요처인 중계기, 기지국, 무선네트워크 구축 장비 업체의 수급 동향, 기술변화, 경영환경변화, 판매실적 등에 민감하게 반응하는 수요구조로 되어 있다. SWOT 분석결과 전력 증폭기 모듈설계 노하우와 하이브리드 전력 증폭기를 개발할 수 있는 강점과 군수용으로 응용 분야 확대 및 5G 상용화의 기회를 활용하여 사업 기회를 선점하는 사업전략 수립이 필요하다. 산업구조 분석결과 구매자의 교섭력이 높으므로 전방산업에 배치된 수요기업과의 전략적이고 긴밀한 협력관계를 유지하기 위한 전략을 추진해야 한다.

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Determination of Brassinolide by HPLC equipped with Fluoresence Detector in Rice(Oriza sativa L.) (HPLC 형광분석법을 통한 벼에서 Brassinolide의 검정)

  • Kim, In-Seon;Lee, Kang-Bong;Suh, Yong-Tack;Morgan, E.D.;Shim, Jae-Han
    • Applied Biological Chemistry
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    • v.39 no.1
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    • pp.84-88
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    • 1996
  • To determine brassinolide in rice(Oriza sativa L.) using HPLC equipped with fluoresence detector, a highly sensitive fluorescence reagent. 1-cyanoisoindole-2-m-phenylboronic acid, was synthesized from the reaction of o-phthaldehyde, m-phenylboronic acid and KCN, then was reacted with brassinolide. The formation ratio of brassinolide boronate exhibited 90% up at the ratio of $20\;:\;1({\mu}g/{\mu}g)$ of 1-cyanoisoindole-2-m-phenylboronic acid and brassinolide respectively. The detection limit of brassinolide boronate with fluoresence detector was 0.16 ng. Brassinolide was detected in heading stage(biomass : 10 g) and panicle formation stage(biomass : 100 g) of the rice(Oryza sativa L.) with quantity of $0.8\;{\mu}g\;and\;0.2\;{\mu}g$respectively. However, brassinolide was not detected in blooming and elongation stage.

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pH-Dependent Dye Adsorption and Release Behaviors of Poly(ethylene-alt-maleic anhydride)/poly(4-vinyl pyridine) Multiplayer Films (pH 의존 특성을 갖는 Poly(ethylene-alt-maleic anhydride)/Poly(4-vinyl pyridine) 다층막의 염료 흡착 및 방출 거동 연구)

  • Hong, Sook-Young;Lee, Joon-Youl
    • Polymer(Korea)
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    • v.29 no.6
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    • pp.593-598
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    • 2005
  • This work studied the loading capabilities and release behaviors of poly(ethylene-alt-maleic anhydride) (PEMAh)/poly(4-vinyl pyridine) (P4VP) multilayer films formed by the layer-by-layer(LbL) sequential self-assembly method, using Rodamine 6G(R6G) as an indicator. Thickness of the multilayer, and loading and subsequent release behavior of R6G from the multilayer were studied using UV-visible spectroscopy. The amount of R6G loaded in multilayer film increased linearly with increasing film thickness. pH-Sensitive permeability was observed, where lower pH environments increased both release rate and release amount. By additional assembling of PEMAh/poly(ethyleneimine) (PEI) capping layers on top of (PEMAh/P4VP)n multilayers, the release of R6G was better controlled.

MAC Scheduling Scheme for VoIP Traffic Service in 3G LTE (3G LTE VoIP 트래픽 서비스를 위한 MAC 스케줄링 기법)

  • Jun, Kyung-Koo
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.32 no.6A
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    • pp.558-564
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    • 2007
  • 3G Long Term Evolution, which aims for various mobile multimedia service provision by enhanced wireless interface, proposes VoIP-based voice service through a Packet Switching (PS) domain. As delay and loss-sensitive VoIP traffic flows through the PS domain, more challenging technical difficulties are expected than in Circuit Switching (CS) domain based VoIP services. Moreover, since 3G LTE, which adopts the OFDM as its physical layer, introduces Physical Resource Block (PRB) as a unit for transmission resources, new types of resource management schemes are needed. This paper proposes a PRB scheduling algorithm of MAC layer for VoIP service in 3G LTE and shows the simulation results. The proposed algorithm has two key parts; dynamic activation of VoIP priority mode to satisfy VoIP QoS requirements and adaptive adjustment of the priority mode duration in order to minimize the degradation of resource utilization.

Ethics-Literacy Curriculum Modeling for Ethical Practice of 5G Information Professionals (5G 정보환경 정보전문가를 위한 윤리 리터러시 교육과정 모형연구)

  • Yoo, Sarah
    • Journal of the Korean BIBLIA Society for library and Information Science
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    • v.33 no.1
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    • pp.139-166
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    • 2022
  • Ethical Issues increase when people engage in smart technological systems such as 5G, IoT, Cloud computing services and AI applications. Range of this research is comparison of various literacy concepts and its ethical issues in considering of 5G features and UX. 86 research papers and reports which have been published within the recent 5 years (2017-2022), relating the research subject, are investigated and analyzed. Two results show that various literacies can be grouped into four areas and that some of common issues among those areas as well as unique issues of each area are identified. Based on the literature analysis, an Operational Definition of Ethics-Literacy is presented and the model of ethics-literacy curriculum supporting ethical behavior of 5G information professionals is developed and suggested.

Shock Simulation and Experimental Verification of HDD (하드디스크 드라이브의 충격해석 및 실험적 검증)

  • Kim, Jin-Gon;Lee, Jae-Kon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.10 no.10
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    • pp.2583-2588
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    • 2009
  • This study deals with the shock response analysis of HDD subjected to a half-sine shock pulse and its experimental verification. Comparatively, accurate computer simulation allows designers to determine complete mechanical information during the product impact time period, compared with only segmental messages by sensors in a test, to predict potential failures. But, impact/shock simulation technology is rather sensitive to various factors to predict the shock behavior without validation. In our shock simulation, the methodology of analysis with LS-DYNA3D and test validation is adopted to predict the shock behavior of HDD. We can confirm the soundness of the present shock simulation through the comparison with electromagnetic shock test(200G/1ms) and linear drop test(300G/2ms).

Extended 3-Party Mutual Authentication Protocols for the Virtual Home Environment in Next Generation Mobile Networks (차세대 이동통신 네트워크의 Virtual Home Environment 구조에 적용 가능한 3자간 상호 인증 프로토콜)

  • Jeong, Jong-Min;Lee, Goo-Yeon;Lee, Yong
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.40 no.4
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    • pp.22-29
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    • 2003
  • In the virtual home environment (VHE), which was proposed to offer global roaming and personal service environment portability, user's profiles and service logics are conveyed from home network to visited network to provide services at the visited network. Because user's profiles and service logics may contain confidential information, some procedures for mutual authentication among entities for offering confidence are needed. For these issues, we propose and analyze three 3-Party mutual authentication Protocols adaptable to the VHE in 3G ; password based mutual authentication protocol, mutual authentication protocol with CHAP and key exchange and mutual authentication protocol with trusted third party.