• Journal of environmental and Sanitary engineering
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• v.13 no.2
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• pp.115-120
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• 1998

This Study was performed to investigate the contents of aflatoxin $B_{1}$ in cereal, pulse, nuts and these products of domestic and import. These results were as follows. 1. Average concentration(in ${\mu}g/kg$) of aflatoxin $B_{1}$ in domestic foods were 2.6 in cereal, 3.9 in pulse, 4.2 in nuts and 1.4 grain products. The contents of aflatoxin B$_{1}$ in pulse and nuts were much higher than those of cereal and grain products. But their values were still within $10{\mu}g/kg$ the maximum residual level of aflatoxin $B_{1}$ for food of Korea. 2. Average contents of aflatoxin $B_{1}$ in import foods were 4.8, 5.4, 6.0, $3.8{\mu}g/kg$ for cereal pulse , nuts and popcorn & peanuts butter, respectively. And these values were found to be below the maximum residual level ($10{\mu}g/kg$) of aflatoxin $B_{1}$ for food of Korea. 3. The concentration of aflatoxin $B_{1}$ in 2 samples of domestic and 9 samples of import foods were over the tolerance limit for aflatoxin $B_{1}$ in food of Korea. Therefore, the hygienic managements of the foods should be required during storage and circulation at market.

• Transactions of the Korean hydrogen and new energy society
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• v.13 no.3
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• pp.181-189
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• 2002

A series of multicomponent $Zr_{0.8}Ti_{0.2}Mn_{0.4}V_{0.6}Ni_{1-x}Fe_{x}$ (x=0.00, 0.08, 0.15, 0.22, and 0.30) alloys are prepared and their oystal structure and P-C-T curves are examined. The electrochemical properties of these allqys such as activation conditions, discharge capacity, cycling performance are also investigated. $Zr_{0.8}Ti_{0.2}Mn_{0.4}V_{0.6}Ni_{1-x}Fe_{x}$ (x=0.00, 0.08, 0.15, 0.22 and 0.30) have the C14 Laves phase hexagonal structure. The electrode was activated by the hot-charging treatment. The best activation conditions were the current density 120 mA/g and the hot-charging time 12h at $80^{\circ}C$ in the case of the alloy with x=0.00. The discharge capacity increased rapidly until the fourth cycle and then decreased. The discharge capacity increased again from the 13th cycle, arriving at 234 mAh/g at the 50th cycle. The discharge capacily just after activation decreases with the increase in the amount of the substituted Fe but the cycling performance is improved. The discharge capacity after activation of the alloy with x=0.00 is 157 mAh/g at the current density 120 mA/g. $Zr_{0.8}Ti_{0.2}Mn_{0.4}V_{0.6}Ni_{0.85}Fe_{0.15}$ is a good composition with a medium quantity of discharge capacities and a good cycling performance. The ICP analysis of the electrolyte for these electrodes after 50 charge-discharge cycles shows that the concentrations of V and Zr are relatively high. Another series of multicomponent $Zr_{0.8}Ti_{0.2}Mn_{0.4}V_{0.6}Ni_{0.85}M_{0.15}$ (M = Fe, Co, Cu, Mo and Al) alloys are prepared. They also have the C14 Laves phase hexagonal structure. The alloys with M = Co and Fe have relatively larger hydrogen storage capacities. The discharge capacities just after activation are relatively large in the case of the alloys with M = Al and Cu. They are 212 and 170 mAh/g, respectivety, at the current density 120mA/g. The $Zr_{0.8}Ti_{0.2}Mn_{0.4}V_{0.6}Ni_{0.85}Co_{0.15}$ alloy is the best one with a relatively large discharge capacity and a good cycling performance.

• Journal of Korean Society of Forest Science
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• v.96 no.1
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• pp.48-53
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• 2007

This study was carried out to investigate the effect of $NO_3{^-}$ and $NH_4{^+}$ on the adventitious root growth and eleuthroside synthesis of Eleutherococcus senticosus during 3 L-bioreactor culture. The change of medium component ratio was also measured during culture. The fresh weignt of adventitious root reached to the greatest level of 24.4g FW/L in the presence of 50 mM $NO_3{^-}$ and 10 mM $NH_4{^+}$, representing 3.4-fold increase compared to the 60 mM $NH_4{^+}$. However, as the increase of the portion of $NH_4{^+}$, the root growth was decreased. Maximum eleutheroside B and E1 production were $249{\mu}g/g$ and $43{\mu}g/g$, respectively, with 30 mM total nitrogen source. The maximum production of eleutheroside E were $788{\mu}g/g$ with 120 mM total nitrogen source. The greatest weight of adventitious root increased up to 6.2 fold of inoculum size within 9 weeks. The change of pH was influenced from 4.81 to 6.35 and the amounts of $NH_4{^+}$ and $K^+$ were decreased during culture periods. From these results we suggest, need further study of various treatment to increase the growth of biomass and the accumulation of useful secondary metabilites.

• Journal of the Korean Wood Science and Technology
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• v.36 no.4
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• pp.37-43
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• 2008

The research attempted to develop an eco-friendly wood adhesive based on vegetable oil (soybean oil), the renewable and sustainable natural resources, using ozonification technology for the chemical structure modification. The soybean oils (SBO) were reacted with $O_3$ at the rate of 7.13 g/h for different times, 15 minutes, 30 minutes, 60 minutes, and 120 minutes. Modified chemical structure of the ozonized SBOs were examined by Fourier transform Infrared (FT-IR) spectrum. The FT-IR spectrum of SBO had an absorbance peak at $3010cm^{-1}$ that is the characteristic peak of the unsaturated double bonds. As ozone treatment time increased, the peak of the double bond was disappeared and aldehyde or carboxyl peak appeared at $1700cm^{-1}$. The dry, wet, and cyclic boiling bond strengths of the ozonized SBO mixed with polymeric diphenylmethane-4, 4-diisocyanate (pMDI) were also investigated. In the dry shear test, all strengths met constantly the standard requirement of $7.0kgf/cm^2$ (KS F3101 2006). The bond strengths gradually increased with increasing ozone treatment time. The highest strength showed at 60 minutes ozone treatment and decreased values at 120 minutes. In the cyclic boiling shear test, 30, 60 and 120 minutes exceeded the standard requirement.

• Journal of Dairy Science and Biotechnology
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• v.14 no.2
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• pp.229-245
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• 1996

1. CM-Sephadex C-50-120 column을 사용하여 HLF를 효과적으로 정제하였다. CM-Sepha-dex 50-120 column chromatography를 수행한 결과, HLF는 NaCl 350 mM-500 mM 사이에서 용출되었으며, 용출된 분획을 SDS-PAGE를 수행해서 단일 band를 확인하였다. Anti-HLF antibody를 이용한 Western blotting 결과 nitrocellulose paper 상에 단일 band 가 나타나므로 HLF 가 효과적으로 정제되었다는 것을 알 수 있었다. 2. Con A, PWM, PWA LPS 등의 자극원으로 단핵구와 대식세포를 자극한 다음 BLF를처리한 배 양액을 IL-1 bioassy한 결과는 Con A 33%, PMA 33%, PWM 15%, LPS 35% 이고, HLF로 처리하여 IL-1 bioassay를 한 결과는 Con A 15%, PMA 22%, PWM 10%, LPS 5%로 감소된 것으로 나타났다. 3. K-562 세포를 이용한 colony forming assay에서 BLF가 10 g/ml일 때는 30%, 30 g/ml일 때는 35%, HLF는 10 g/ml일 때는 5%,30 g/ml일 때는 30%의 저해를 나타냈다. 4. Lactoferrin의 면역증강효과를 알아보기 위하여 hapten인 VCR-BSA를 투여 한 후, 생성되는 항체생성능력을 ELISA로 비교하였다. 그 결과 HLF 및 BLF 투여군에서 대조군에 비하여 adjuvant 효과를 관찰할 수 있었다. 또한 이때 macrophage 수는 대조군에 비하여 HLF와 BLF를 투여한 군이 증가됨을 알 수 있었다. 이러한 결과로 미루어 볼 때 LF는 macrophage를 활성화 시켜서 항체 생성 능력을 증가시키는 효과가 있음을 관찰할 수 있었다. 5. Balb/c mouse의 thymus로부터 분리한 CD4- CD8- 세포를 BLF로 처리하여 24 시간 배양한 후 CB4$^-$ CD8$^-$ 세포의 분화를 측정한 결과, CD4$^-$를 CD4$^+$ 로 분화하였다. 그리고 HLF로 처리하여 24 시간 배양 후 CD4$^-$ CD8$^-$ 세포의 분화를 측정한 결과, CD4$^-$ CD8$^-$를 CD4$^+$ CD8$^+$ 로 분화하였다. 6. Lactoferrin이 T cell의 IL-2 production에 미치는 영향은 PMA 처리군, PMA+OKT3처리군, LF 단독 처리군 보다 PMA+OKT3+LF를 처리한 군이 IL-2 생성에 영향을 미쳤다. 7. Lfcin B의 단일크론항체에 의해 인식되어지는 Lfcin B의 항원결정기는 ‘QWR’로 밝혀졌다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.7
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• pp.986-990
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• 2003

The living Glass puffers, Takifugu niphobles (Bogseom as Korean name), 10 specimens, were purchased at Tongyeong traditional fisheries market every month from November, 2001 to October, 2002, and tested anatomical distribution of the toxicity such as muscle, skin, intestine, liver and ovary by mouse bioassay method. Significant seasonal variations of the toxicity were not shown in muscle, skin, intestine and liver except having lower toxicity in spawning season (May and June) than non-spawning seasons. 92 specimens (76.7%) out of 120 ones were non toxic (below 10 MU/g) for the edible and 28 ones (23.3%) were weakly toxic (10 MU/g∼l00 MU/g) for the non-edible in muscle ranged between 0 MU/g and 46.4 MU/g. All of those toxicity were shown over 10 MU/g in skin for the non-edible in skin ranged from 10.0 MU/g to 674.4 MU/g. Toxicity range in intestine and liver was varied between non-toxic and strongly toxic (over 1,000 MU/g) for individual specimens. Toxicity range of the ovary found only in May (7 specimens) and June (1 specimens) was 84.2 MU/g to 2,191.3 MU/g.

• The Korean Journal of Food And Nutrition
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• v.27 no.5
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• pp.897-905
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• 2014

The foliage of sweet potato [Impomoea batatas (L.) Lam] is an excellent source of food material due to the functional components of polyphenol and carotenoid. In this study, the lightness (L-value), polyphenol contents, and DPPH radical scavenging activity of the foliage of Shinwhangmi, Hayanmi and Suioh cultivars harvested after different cultivation periods (60, 90 and 120 days after planting) and according to tip locations (1st~5th, 6th~10th, 11th~15th tips) were investigated. As a result, Shinwhangmi showed a lower lightness value (60 days after planting and 1st~5th tips). The longer the cultivation periods and the closer the tips, the higher the contents of polyphenol and DPPH radical scavenging activity were in lyophilized foliage of Shinwhangmi, Hayanmi and Suioh. In the 1st~10th tip of Shinwhangmi, Hayanmi and Suioh cultivated for 90 days and dried by 2 step hot-air (1st: $70^{\circ}C$ for 8 hr, 2nd: $80^{\circ}C$ for 4 hr), the polyphenol and ${\beta}$-carotenoid contents were 5.0, 3.9 and 4.0 mg/g and 75.6, 71.6 and 63.1 mg/g, respectively. The DPPH radical scavenging activities and ABTS radical scavenging activities ($EC_{50}$) were 0.29, 0.36 and 0.33 mg and 0.12, 0.15 and 0.11 mg, respectively. These antioxidative activities were over two times stronger than spinach.

• The Korean Journal of Community Living Science
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• v.16 no.2
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• pp.93-97
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• 2005

This study was conducted to clarify the morphological characteristics of Goheung native garlic variety compared with those of Spain and Taiwan varieties in Goheung region. Bulb fresh weights of Goheung, Spain and Taiwan varieties were 59.1g, 120.3g and 78.1g. Those bulb diameters were as followed that Spain 8.1cm, Taiwan 7.2cm and Goheung 5.3cm. And, bulb heights were Spain 5.4cm, Taiwan 4.4cm and Goheung 4.0cm. Bulb shape index of garlics by different varieties were increased in the order of Taiwan, Spain and Goheung products. Number of cloves produced in garlics were in the order of Goheung (12.1), Taiwan (11.4) and Spain (8.2) products. And clove fresh weight of garlics were Spain (7.7g), Taiwan (6.8g) and Goheung (4.6g) products. Clove height of garlics were in the order of Spain (2.8cm), Taiwan (2.4cm) and Goheung (2.1cm) products. And, clove diameter of garlics were Spain (2.1cm), Taiwan (1.7cm) and Goheung (1.3cm) products. Clove shape index of garlics by different varieties were increased in the order of Goheung (1.62), Taiwan (1.45) and Spain (1.32) products. Skin color differences in terms of a value were increased in the order of Goheung, Taiwan and Spain varieties. Surface color differences of flesh texture and peeled clove for garlics by the different garlic varieties were not significant.

• Journal of Plant Biotechnology
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• v.46 no.2
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• pp.119-126
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• 2019

This study was conducted to investigate the optimal culture method for gametophyte and sporophyte propagation in Coniogramme japonica (Thunb.) Diels, which can be used in various fields. The propagation of prothallus were cultured in 1/4 - 1 Murashige and Skoog medium and Knop medium for 10 weeks. The results indicated that the fresh weight of prothallus was the highest (14.5 g) in 1MS medium. Subsequently, various concentrations of sucrose, activated charcoal and nitrogen source were also added to 1MS medium and cultured for 8 weeks. The results provided that the sucrose concentration was 3% and the fresh weight of prothallus was the highest 10.8 g. According to the concentration in the range of 8.8 ~ 10.8 g, in the case of activated charcoal, the four treatments showed no significant difference. The nitrogen source was added at a concentration of 30, 60 and 120 mM with the ratio of ${NH_4}^+:{NO_3}^-$ being 1 : 2. As a result, the fresh weight of all treatments increased to similar level but there was no significant difference. We investigated sporophyte formation according to soil type and the highest number of sporophytes at 228.0 was formed in soils mixed with horticultural substrate and decomposed granite at 2 : 1 (v : v). On the other hand, sporophyte was not formed in soils containing peatmoss except for the one with peatmoss and decomposed granite at 2 : 1 (v : v).

• Korean Journal of Veterinary Research
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• v.36 no.2
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• pp.471-482
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• 1996

These experiments were undertaken in order to find out the useful clinicopathological diagnostic methods of cadmium poisoning in dogs. Twenty-one dogs were divided into a control group and 6 experimental groups. The experimental groups were adminstered orally 5, 10, 15, 30, 60 and 120mg of cadmium per kg of body weight for 56 days. All dogs were examined for clinical signs, and weekly changes in hematological and blood chemical values. All dogs were necropsied on 57th days of experiment. Tissue samples including hair, skin, muscle, lung, liver, kidney, spleen, pancreas, testis, ovary, uterus, and bone were collected and analyzed for cadmium, zinc, iron and copper contents using atomic absorption spectrophotometer. From these experiments following results were obtained : 1. All experimental dogs showed vomitting, salivation, anorexia, decreased water-intake, dehydration, and marked weight loss. The dogs received 30mg/kg or more of cadmium died during the period from 2nd to 7th week after administration. 2. Hematologically, all experimental dogs showed decrease in erythrocyte count, hemoglobin concentration, and packed cell volume. The anemia was identified as normocytic and regenerative morphologically. 3. No significant differences in serum glutamic oxaloacetic transminase, glutamic pyruvic transaminase, blood urea nitrogen, and cholosterol value were obseved between the control and experimental dogs. 4. The cadmium contents in various tissues of experimental dogs were estimated as $37.8{\sim}201.8{\mu}g/g$ in bone, $14.1{\sim}49.5{\mu}g/g$ in liver, $13.2{\sim}53.1{\mu}g/g$ in kidney, $0.4{\sim}35.2{\mu}g/g$ in pancreas, $0.8{\sim}35.4{\mu}g/g$ in spleen, $0.9{\sim}30.1{\mu}g/g$ in hair, $0{\sim}7.1{\mu}g/g$ in lung, $0{\sim}5.1{\mu}g/g$ in skin, and $0{\sim}3.6{\mu}g/g$ in muscle, respectively. However, the serum, testis, ovary and uterus showed no cadmium accumulation. Two contol dogs showed cadmium accumulation only in bone. 5. Significant differances in zinc, iron, and copper contents in tissue samples were observed between the control and experimental groups.