• 제목/요약/키워드: Fusion strategy

검색결과 183건 처리시간 0.027초

Lumbar Interbody Fusion Outcomes in Degenerative Lumbar Disease : Comparison of Results between Patients Over and Under 65 Years of Age

  • Jo, Dae-Jean;Jun, Jae-Kyun;Kim, Ki-Tack;Kim, Sung-Min
    • Journal of Korean Neurosurgical Society
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    • 제48권5호
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    • pp.412-418
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    • 2010
  • Objective : To evaluate the clinical and radiological outcomes of lumbar interbody fusion and its correlation with various factors (e.g., age, comorbidities, fusion level, bone quality) in patients over and under 65 years of age who underwent lumbar fusion surgery for degenerative lumbar disease. Methods : One-hundred-thirty-three patients with lumbar degenerative disease underwent lumbar fusion surgery between June 2006 and June 2007 and were followed for more than one year. Forty-eight (361%) were older than 65 years of age (group A) and 85 (63.9%) were under 65 years of age (group B). Diagnosis, comorbidities, length of hospital stay, and perioperative complications were recorded. The analysis of clinical outcomes was based on the visual analogue scale (VAS). Radiological results were evaluated using plain radiographs. Clinical outcomes, radiological outcomes, length of hospital stay, and complication rates were analyzed in relation to lumbar fusion level, the number of comorbidities, bone mineral density (BMD), and age. Results : The mean age of the patients was 61.2 years (range, 33-86 years) and the mean BMD was -2.2 (range, -4.8 to -2.8). The mean length of hospital stay was 15.0 days (range, 5-60 days) and the mean follow-up was 23.0 months (range, 18-30 months). Eighty-five (64.0%) patients had more than one preoperative comorbidities. Perioperative complications occurred in 27 of 133 patients (20.3%). The incidence of overall complication was 22.9% in group A, and 18.8% in group B but there was no statistical difference between the two groups. The mean VAS scores for the back and leg were significantly decreased in both groups (p < 0.05), and bony fusion was achieved in 125 of 133 patients (94.0%). There was no significant difference in bony union rates between groups A and B (91.7% in group A vs. 95.3% in group B, p = 0.398). In group A. perioperative complications were more common with the increase in fusion level (p = 0.027). Perioperative complications in both groups A (p = 0.035) and B (p = 0.044) increased with an increasing number of comorbidities. Conclusion : Elderly patients with comorbidities are at a high risk for complications and adverse outcomes after lumbar spine surgery. In our study, clinical outcomes, fusion rates, and perioperative complication rates in older patients were comparable with those in younger populations. The number of comorbidities and the extent of fusion level were significant factors in predicting the occurrence of postoperative complications. However, proper perioperative general supportive care with a thorough fusion strategy during the operation could improve the overall postoperative outcomes in lumbar fusion surgery for elderly patients.

In Vivo Protein Transduction: Delivery of PEP-1-SOD1 Fusion Protein into Myocardium Efficiently Protects against Ischemic Insult

  • Zhang, You-en;Wang, Jia-ning;Tang, Jun-ming;Guo, Ling-yun;Yang, Jian-ye;Huang, Yong-zhang;Tan, Yan;Fu, Shou-zhi;Kong, Xia;Zheng, Fei
    • Molecules and Cells
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    • 제27권2호
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    • pp.159-166
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    • 2009
  • Myocardial ischemia-reperfusion injury is a medical problem occurring as damage to the myocardium following blood flow restoration after a critical period of coronary occlusion. Oxygen free radicals (OFR) are implicated in reperfusion injury after myocardial ischemia. The antioxidant enzyme, Cu, Zn-superoxide dismutase (Cu, Zn-SOD, also called SOD1) is one of the major means by which cells counteract the deleterious effects of OFR after ischemia. Recently, we reported that a PEP-1-SOD1 fusion protein was efficiently delivered into cultured cells and isolated rat hearts with ischemia-reperfusion injury. In the present study, we investigated the protective effects of the PEP-1-SOD1 fusion protein after ischemic insult. Immunofluorescecnce analysis revealed that the expressed and purified PEP-1-SOD1 fusion protein injected into rat tail veins was efficiently transduced into the myocardium with its native protein structure intact. When injected into Sprague-Dawley rat tail veins, the PEP-1-SOD1 fusion protein significantly attenuated myocardial ischemia-reperfusion damage; characterized by improving cardiac function of the left ventricle, decreasing infarct size, reducing the level of malondialdehyde (MDA), decreasing the release of creatine kinase (CK) and lactate dehydrogenase (LDH), and relieving cardiomyocyte apoptosis. These results suggest that the biologically active intact forms of PEP-1-SOD1 fusion protein will provide an efficient strategy for therapeutic delivery in various diseases related to SOD1 or to OFR.

In Vivo Expression Technology (IVET) and Its Application in Plant-Associated Bacteria

  • Lee, Seon-Woo
    • The Plant Pathology Journal
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    • 제18권2호
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    • pp.57-62
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    • 2002
  • In vivo expression technology (IVET) has been developed to study bacterial gene expression in Salmonella typhimurium during host infection. The expression of selected genes by IVET has been elevated in vivo but not in vitro. The selected genes turned out to be important for bacterial virulence and/or pathogenicity. IVET depends on a synthetic operon with a promoterless transcriptional fusion between a selection marker gene and a reporter gene. The IVET approach has been successfully adapted in other bacterial pathogens and plant-associated bacteria using different selection markers. Pseudomonas putida suppresses citrus root rot caused by Phytophthora parasitica and enhances citrus seedling growth. The WET strategy was adapted based on a transcriptional fusion, pyrBC'-lacZ, in P. putida to study the bacterial traits important far biocontrol activities. Several genes appeared to be induced on P. parasitica hyphae and were found to be related with metabolism and regulation of gene expression. It is likely that the biocontrol strain took a metabolic advantage from the plant pathogenic fungus and then suppressed citrus root rot effectively. The result was parallel with those from the adaptation of IVET in P. fluorescens, a plant growth promoting rhizobacteria (PGPR). Interestingly, genes encoding components for type III secretion system have been identified as rhizosphere-induced genes in the PGPR strain. The type III secretion system may play a certain role during interaction with its counterpart plants. Application of IVET has been demonstrated in a wide range of bacteria. It is an important strategy to genetically understand complicated bacterial traits in the environment.

Display of Proteins on the Surface of Escherichia coli by C-Terminal Deletion Fusion to the Salmonella typhimurium OmpC

  • CHOI JONG-HYUN;CHOI, JONG-IL;LEE, SANG-YUP
    • Journal of Microbiology and Biotechnology
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    • 제15권1호
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    • pp.141-146
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    • 2005
  • A new system for displaying proteins on the surface of Escherichia coli was developed using the Salmonella typhimurium outer membrane protein C (OmpC) as an anchoring motif. The C-terminal deletionfusion strategy was developed to fuse the polyhistidine peptides and green fluorescent protein (GFP) to the Cterminal of the truncated functional portion of OmpC. The polyhistidine peptides of up to 243 amino acids could besuccessfully displayed on the E. coli cell surface, which allowed recombinant E. coli to adsorb up to 34.2 μmol of Cd2+ per gram dry cell weight. The GFP could also be successfully displayed on the E. coli cell surface. These results suggest that the C-terminal deletion-fusion strategy employing the S. typhimurium OmpC as an anchoring motif provides a new efficient way for the display of large proteins on the surface of E. coli.

DTW와 퓨전기법을 이용한 비유사도 기반 분류법의 최적화 (On Optimizing Dissimilarity-Based Classifications Using a DTW and Fusion Strategies)

  • 김상운;김승환
    • 전자공학회논문지CI
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    • 제47권2호
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    • pp.21-28
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    • 2010
  • 본 논문에서는 동적시간교정법(dynamic time warping: DTW)과 다중퓨전기법(multiple fusion strategy: MFS)을 연속 적용하여 비유사도기반 분류법(dissimilarity-based classification: DBC)을 최적화시키는 방법의 실험결과를 보고한다. DBC란 샘플패턴을 분류하기 위하여 샘플의 특징 값을 이용하는 대신에 샘플들 사이의 비유사도를 측정하여 분류기를 설계하는 방법이다. DTW에서는 다음과 같이 두 단계로 나누어 비유사도를 측정한다. 먼저 상관계수를 이용하여 객체 샘플들을 대응시키기 위한 최적의 대응경로를 찾을 수 있도록 샘플들을 조정한다. 그리고 기존의 거리측정법으로 조정된 샘플들 사이의 비유사도를 측정한다. MFS에서는 분류기결합 뿐만 아니라 비유사도 행렬생성에서도 퓨전기법을 적용한다. 즉, DTW 기법으로 작성한 다수의 비유사도 행렬들을 결합하여 새로운 비유사도 행렬을 생성한 다음, 이 행렬공간에서 여러 개의 베이스 분류기를 학습하여 다시 결합한다. 본 논문에서 제안한 방법을 벤취마크 영상 데이터베이스를 대상으로 실험한 결과, 기존의 방법과 비교하여 분류성능을 향상시킬 수 있음을 확인하였다. 이와 같은 실험결과로 볼 때, 제안 방법을 멀티미디어 정보검색 등과 같은 다른 고차원 응용에도 활용할 수 있을 것으로 사료된다.

Crack location in beams by data fusion of fractal dimension features of laser-measured operating deflection shapes

  • Bai, R.B.;Song, X.G.;Radzienski, M.;Cao, M.S.;Ostachowicz, W.;Wang, S.S.
    • Smart Structures and Systems
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    • 제13권6호
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    • pp.975-991
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    • 2014
  • The objective of this study is to develop a reliable method for locating cracks in a beam using data fusion of fractal dimension features of operating deflection shapes. The Katz's fractal dimension curve of an operating deflection shape is used as a basic feature of damage. Like most available damage features, the Katz's fractal dimension curve has a notable limitation in characterizing damage: it is unresponsive to damage near the nodes of structural deformation responses, e.g., operating deflection shapes. To address this limitation, data fusion of Katz's fractal dimension curves of various operating deflection shapes is used to create a sophisticated fractal damage feature, the 'overall Katz's fractal dimension curve'. This overall Katz's fractal dimension curve has the distinctive capability of overcoming the nodal effect of operating deflection shapes so that it maximizes responsiveness to damage and reliability of damage localization. The method is applied to the detection of damage in numerical and experimental cases of cantilever beams with single/multiple cracks, with high-resolution operating deflection shapes acquired by a scanning laser vibrometer. Results show that the overall Katz's fractal dimension curve can locate single/multiple cracks in beams with significantly improved accuracy and reliability in comparison to the existing method. Data fusion of fractal dimension features of operating deflection shapes provides a viable strategy for identifying damage in beam-type structures, with robustness against node effects.

다단계 퓨전기법을 이용한 비유사도 기반 식별기의 최적화 (On Optimizing Dissimilarity-Based Classifier Using Multi-level Fusion Strategies)

  • 김상운;로버트 듀인
    • 전자공학회논문지CI
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    • 제45권5호
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    • pp.15-24
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    • 2008
  • 얼굴인식 등과 같은 고차원 식별문제에서는 샘플패턴의 수가 패턴의 차원보다 작아지게 된다. 이러한 상황에서 차원을 축소하기위해 선형판별분석법을 적용할 경우, 희소성(Small Sample Size: SSS)문제가 발생한다. 최근, SSS 문제를 해결하기 위하여 비유사도에 기반 한 식별법(Dissimilarity-Based Classification: DBC)을 이용하는 방법이 검토되었다. DBC에서는 특징 벡터 대신에 학습 샘플들로부터 추출한 프로토타입들과의 비유사도를 측정하여 입력 패턴을 식별하는 방법이다. 본 논문에서는 비유사도 표현단계와 DBC 학습단계에서 퓨전기법을 중복 적용하는 다단계 퓨전기법(Multi-level Fusion Strategies: MFS)으로 DBCs를 최적화시키는 방법을 제안한다. 제안 방법을 벤취마크 얼굴영상 데이터베이스를 대상으로 실험한 결과, 식별률을 향상시킬 수 있음을 확인하였다.

Characterization of KRC-108 as a TrkA Kinase Inhibitor with Anti-Tumor Effects

  • Lee, Hyo Jeong;Moon, Yeongyu;Choi, Jungil;Heo, Jeong Doo;Kim, Sekwang;Nallapaneni, Hari Krishna;Chin, Young-Won;Lee, Jongkook;Han, Sun-Young
    • Biomolecules & Therapeutics
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    • 제30권4호
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    • pp.360-367
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    • 2022
  • Tropomyosin receptor kinase A (TrkA) protein is a receptor tyrosine kinase encoded by the NTRK1 gene. TrkA signaling mediates the proliferation, differentiation, and survival of neurons and other cells following stimulation by its ligand, the nerve growth factor. Chromosomal rearrangements of the NTRK1 gene result in the generation of TrkA fusion protein, which is known to cause deregulation of TrkA signaling. Targeting TrkA activity represents a promising strategy for the treatment of cancers that harbor the TrkA fusion protein. In this study, we evaluated the TrkA-inhibitory activity of the benzoxazole compound KRC-108. KRC-108 inhibited TrkA activity in an in vitro kinase assay, and suppressed the growth of KM12C colon cancer cells harboring an NTRK1 gene fusion. KRC-108 treatment induced cell cycle arrest, apoptotic cell death, and autophagy. KRC-108 suppressed the phosphorylation of downstream signaling molecules of TrkA, including Akt, phospholipase Cγ, and ERK1/2. Furthermore, KRC-108 exhibited antitumor activity in vivo in a KM12C cell xenograft model. These results indicate that KRC-108 may be a promising therapeutic agent for Trk fusion-positive cancers.

직각정규화와 DEM 자료 융합을 이용한 광역 DEM 생성 (A Wide DEM Generation Based on Orthoretification and DEM Data Fusion)

  • 예철수;전병민;이쾌희
    • 대한원격탐사학회지
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    • 제16권1호
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    • pp.99-108
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    • 2000
  • 본 논문에서는 SPOT 위성 영상을 사용하여 얻어진 수치 표고 모형을 결합하는 기법을 제안한다. 추출된 수치 표고 모형의 결과를 사용하여 위도와 경도로 이루어지는 격자를 생성한다. 생성된 격자 위치에서의 고도 값은 이미 획득된 수치 표고 모형으로부터 세 개의 가장 근접한 화소의 고도 값을 이용하는 삼각형 영상 워핑 방법을 사용하여 보간한다. 각 격자의 최종 고도 값은 최대 가능성 규칙의 융합 방법을 이용하여 결정한다 본 논문에 사용된 영상은 두 쌍의 스테레오 SPOT 위성영상을 사용하였으며 실험결과 수치 표고 모형의 고도 값을 성공적으로 융합할 수 있었다.