• Journal of Pharmaceutical Investigation
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• 제38권6호
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• pp.357-363
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• 2008

Penetration rate of large molecule through skin is very low due to the barrier effect of stratum corneum. Novel microneedle treatment device with roll was designed for transdermal delivery of large molecular drugs such as vaccine and protein drugs. The permeation rates of FITC labelled bovine serum albumin (FITC-BSA) as a model protein were determined using modified Franz diffusion cell and hairless mouse skin which were treated by hydrogel or solution containing FITC-BSA. Fluorescent spectrophotometer was used to analyze the concentration of FITC-BSA. Microscope using fluorescent filter was used to capture the image and location of FITC-BSA in the skin. We confirmed that permeation rate of BSA was increased with the treatment by microneedle and was increased by the increasing frequency of treatment. Furthermore, the permeation rate observed from hydrogel treated skin was significantly higher than that from solution treated skin.

• Reproductive and Developmental Biology
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• 제35권1호
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• pp.17-22
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• 2011

This study was designed to determine the effect of electric field strength, duration and fusion buffer in fusion parameters on the rate of membrane fusion between the somatic cell and cytoplast for Korean cattle (HanWoo) somatic cell nuclear transfer (SCNT) procedure. Following electrofusion, effect of 5 or $10\;{\mu}M$ $Ca^{2+}$-ionophore of activation treatment on subsequent development was also evaluated. Cell fusion rates were significantly increased from 23.1% at 20 V/mm to 59.7% at 26 V/mm and 52.9% at 27 V/mm (p<0.05). Due to higher cytoplasmic membrane rupture or cellular lysis, overall efficiency was decreased when the strength was increased to 30 V/mm (18.5%) and 40 V/mm (6.3%) and the fusion rate was also decreased when the strength was at 25 V/mm or below. The optimal duration of electric stimulation was significantly higher in $25\;{\mu}s$ than 20 and $30\;{\mu}s$ (18.5% versus 9.3% and 6.3%, respectively, p<0.05). Two nonelectrolyte fusion buffers, Zimmermann's (0.28 M sucrose) and 0.28 M mannitol solution for cell fusion, were used for donor cell and ooplast fusion and the fusion rate was significantly higher in Zimmermann's cell fusion buffer than in 0.28 M mannitol (91.1% versus 48.4%, respectively, p<0.05). The cleavage and blastocyst formation rates of SCNT bovine embryos activated by $5\;{\mu}M$ $Ca^{2+}$-ionophore was significantly higher than the rates of the embryos activated with $10\;{\mu}M$ of $Ca^{2+}$-ionophore (70.0% versus 42.9% and 22.5% versus 14.3%, respectively; p<0.05). This result is the reverse to that of parthenotes which shows significantly higher cleavage and blastocyst rates in $10\;{\mu}M$ $Ca^{2+}$-ionophore than $5\;{\mu}M$ counterpart (65.6% versus 40.3% and 19.5% versus 9.7%, respectively; p<0.05). In conclusion, SCNT couplet fusion by single pulse of 26 V/mm for $25\;{\mu}s$ in Zimmermann's fusion buffer followed by artificial activation with $5\;{\mu}M$ $Ca^{2+}$-ionophore are suggested as optimal fusion and activation methods in Korean cattle SCNT protocol.

• International Journal of Costume and Fashion
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• 제11권2호
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• pp.13-24
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• 2011

This study explores the opportunities for new market of traditional Korean costumes, Hanbok, following not only the consumer's needs and wants, but marketing trends of fashion industry. This paper is the exploratory consumer research of fusion Hanbok, it aims at examining how consumers wear fusion Hanbok. Study participants were limited to mothers who wore Korean-fusion Hanbok to their baby's first birthday party once or more. A total of 221 respondents completed online questionnaires. The results of this study are as follows. First, regarding the reasons of wearing fusion Hanbok is a beautiful and traditional dress although traditional Hanbok is uncomfortable and inconvenient. Second, the most satisfactory aspect of fusion Hanbok was found to be its design. Satisfaction with the color, size and length was also high. Third, most female consumers in their 20s and 30s have stronger preferences for and intentions of purchasing fusion Hanbok. Some people criticize that as fusion Hanbok is westernized in its designs and materials, it has lost the classical beauty and impression of traditional Hanbok. However, if no one routinely wears Hanbok, Hanbok will never be popularized or globalized. This study suggest that traditional Hanbok should be improved by developing effective merchandising strategies based on consumer needs for traditional Hanbok such as various product assortment and promotion strategies using traditional Korean beauty. So, traditional Hanbok could be retained and succeeded in the future by improving consumers' dissatisfactions of traditional Hanbok and creating the new market of traditional Hanbok in which fusion Hanbok are produced focused on consumers' needs and market environments.

• Journal of Radiation Protection and Research
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• 제37권2호
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• pp.63-69
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• 2012

The second phase of the national program for fusion energy development in Korea starts from 2012 for design and construction of the fusion DEMO reactor. Radiological assessment for the fusion reactor is one of the key tasks to assure its licensability and the starting point of the assessment is determination of the source terms. As the first effort, the activities of the coolant due to activated corrosion product (ACP) were estimated. Data and experiences from fission reactors were used, in part, in the calculations of the ACP concentrations because of lack of operating experience for fusion reactors. The MCNPX code was used to determine neutron spectra and intensities at the coolant locations and the FISPACT code was used to estimate the ACP activities in the coolant of the fusion DEMO reactor. The calculated specific activities of the most nuclides in the fusion DEMO reactor coolant were 2-15 times lower than those in the PWR coolant, but the specific activities of $^{57}Co$ and $^{57}Ni$ were expected to be much higher than in the PWR coolant. The preliminary results of this study can be used to figure out the approximate radiological conditions and to establish a tentative set of radiological design criteria for the systems carrying coolant in the design phase of the fusion DEMO reactor.

• Proceedings of the Korean Vacuum Society Conference
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• 한국진공학회 2006년도 제31회 학술논문발표회 초록집
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• pp.214-214
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• 2006

• Journal of Navigation and Port Research
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• 제27권3호
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• pp.273-282
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• 2003

This paper proposes a sensor-fusion technique where rho data sets for the previous moments are properly transformed and fused into the current data sets to enable accurate measurement, such as, distance to an obstacle and location of the service robot itself. In the conventional fusion schemes, the measurement is dependent only on the current data sets. As the results, more of sensors are required to measure a certain physical promoter or to improve the accuracy of the measurement. However, in this approach, intend of adding more sensors to the system, the temporal sequence of the data sets are stored and utilized for the measurement improvement. Theoretical basis is illustrated by examples md the effectiveness is proved through the simulation. Finally, the new space and time sensor fusion (STSF) scheme is applied to the control of a mobile robot in the indoor environment and the performance was demonstrated by the real experiments.

• Biotechnology and Bioprocess Engineering:BBE
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• 제5권1호
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• pp.13-16
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• 2000

Simple procedures have been devised for purifying recombinant human interleukin-2 (hIL-2), which was expressed in Escberichia coli using sequences of glucagon molecules and enterokinase cleavage site as an N-terminus fusion partner. The insoluble aggregates of recombinant fusion protein produced in E. coli cytoplasm were easily dissolved by simple alkaline pH shift $(8\rightarrow12\rightarrow8)$. Following enterokinase cleavage, the recombinant hIL-2 was finally purified by one-step reversed-phase HPLC with high purity. The ease and high efficiency of this simple purification process seem to mainly result from the role of used glucagon fusion partner, which could be applied to the production of other therapeutically important proteins.

• Journal of Korean Institute of Industrial Engineers
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• 제39권3호
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• pp.198-203
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• 2013

Industry convergence is the inexorable trend, which has become a fundamental concept to understand the industrial dynamics and to develop business strategies. However, most of the previous studies on convergence have dealt with the issues at the macro-level (technology- or industry-level), little attention has been paid to the analysis of convergence at the micro level (firm-level). Recognizing that firms are the principal agents that develop fusion technologies, it is encouraged to help firms to work together for technology convergence. Therefore, this research proposes a collaboration model for SMEs, since SMEs tend to have novel ideas and are flexible enough to make fusion-technology. To do this, we conducted a survey for Korean SMEs and analyzed their successful cases of collaboration, which was used as a basis to develop the model. The research results will help develop strategy or policy to promote the collaboration between SMEs and ultimately to make a fusion-technology.

• Journal of Applied and Pure Mathematics
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• 제5권5_6호
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• pp.389-406
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• 2023

In the pursuit of enhancing image fusion techniques, this research presents a novel approach for fusing multimodal images, specifically infrared (IR) and visible (VIS) images, utilizing a combination of partial differential equations (PDE) and discrete cosine transformation (DCT). The proposed method seeks to leverage the thermal and structural information provided by IR imaging and the fine-grained details offered by VIS imaging create composite images that are superior in quality and informativeness. Through a meticulous fusion process, which involves PDE-guided fusion, DCT component selection, and weighted combination, the methodology aims to strike a balance that optimally preserves essential features and minimizes artifacts. Rigorous evaluations, both objective and subjective, are conducted to validate the effectiveness of the approach. This research contributes to the ongoing advancement of multimodal image fusion, addressing applications in fields like medical imaging, surveillance, and remote sensing, where the marriage of IR and VIS data is of paramount importance.

• Korean Chemical Engineering Research
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• 제46권2호
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• pp.211-216
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• 2008

Bufexamac which was used for treatment of atopic dermatitis is the drug which was made as the ointment. However, penetration rate of bufexamac was very low for the barrier effect of stratum corneum. Microneedle was used to increase transdermal delivery rate of the bufexamac. We tried to conjugate bufexamac and FITC for the detection of penetration rate of bufexamac. FITC-bufexamac was mixed in hydrogel for the treatment skin surface. Fluorescent spectrophotometer was used to analysis the concentration of FITC-bufexamac. Microscope using fluorescent filter was used to capture the image about location of FITC-bufexamac in the skin. We confirmed that permeation rate of bufexamac was increased with the treatment by microneedle and was increased by the increasing number treatment of microneedle.