Journal of Pharmaceutical Investigation

The Korean Society of Pharmaceutical Sciences and Technology (한국약제학회)
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Transdermal Permeation Behavior of FITC-BSA using Microneedle

마이크로니들을 이용한 FITC-BSA의 경피투과 거동

  • Kim, Yun-Tae (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University) ;
  • Young, Oh-A (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University) ;
  • Lee, Jun-Hee (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University) ;
  • Ahn, Sik-Il (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University) ;
  • Park, Jong-Hak (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University) ;
  • Lee, Han-Koo (Research Center, Daehwa Pharm. Co. Ltd.) ;
  • Khang, Gil-Son (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University)
  • 김윤태 (전북대학교 BK-21 고분자 BIN 융합연구팀) ;
  • 오아영 (전북대학교 BK-21 고분자 BIN 융합연구팀) ;
  • 이준희 (전북대학교 BK-21 고분자 BIN 융합연구팀) ;
  • 안식일 (전북대학교 BK-21 고분자 BIN 융합연구팀) ;
  • 박종학 (전북대학교 BK-21 고분자 BIN 융합연구팀) ;
  • 이한구 (한국화학연구원 융합바이오 연구센터) ;
  • 강길선 (전북대학교 BK-21 고분자 BIN 융합연구팀)
  • Published : 2008.12.20
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Abstract

Penetration rate of large molecule through skin is very low due to the barrier effect of stratum corneum. Novel microneedle treatment device with roll was designed for transdermal delivery of large molecular drugs such as vaccine and protein drugs. The permeation rates of FITC labelled bovine serum albumin (FITC-BSA) as a model protein were determined using modified Franz diffusion cell and hairless mouse skin which were treated by hydrogel or solution containing FITC-BSA. Fluorescent spectrophotometer was used to analyze the concentration of FITC-BSA. Microscope using fluorescent filter was used to capture the image and location of FITC-BSA in the skin. We confirmed that permeation rate of BSA was increased with the treatment by microneedle and was increased by the increasing frequency of treatment. Furthermore, the permeation rate observed from hydrogel treated skin was significantly higher than that from solution treated skin.

Keywords

References

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