• Journal of the Korean Chemical Society
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• v.10 no.2
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• pp.98-102
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• 1966

A spectrochemical method for the determination of the major impurities, such as aluminum, iron, magnesium and silicon, in rutile single crystals and variously doped rutile is presented. By applying higher current (12 amp) and a 1:2 sample-to-graphite dilution by weight, the elaborate sample preparation needed for conventional fusion technique was avoided, and relatively higher detection limits were established. Average deviations are approximately ${\pm}8%$ for iron and magnesium in the concentration ranges of 0.007 to 0.7% and 0.006 to 0.6% respectively, and ${\pm}5%$ for aluminum and silicon in the range of 0.005 to 0.5%.

• BMB Reports
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• v.43 no.5
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• pp.319-324
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• 2010

Experimental bioinformatics data obtained from an E. coli cell-based eukaryotic protein purification experiment were analyzed in order to identify any bottleneck as well as the factors affecting the target purification. All targets were expressed as His-tagged maltose-binding protein (MBP) fusion constructs and were initially purified by immobilized metal affinity chromatography (IMAC). The targets were subsequently separated from the His-tagged MBP through TEV protease cleavage followed by a second IMAC isolation. Of the 743 total purification trials, 342 yielded more than 3 mg of target proteins for structural studies. The major reason for failure of target purification was poor TEV proteolysis. The overall success rate for target purification decreased linearly as cysteine content or isoelectric point (pI) of the target increased. This pattern of pI versus overall success rate strongly suggests that pI should be incorporated into target scoring criteria with a threshold value.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.480-483
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• 2003

Fusion ferritin$(F_H+F_L)$, an iron-binding protein, was purified from recombinant E. coli by two-step sonications with urea. Unfolded ferritin was refolded by gel filtration chromatography with various concentration of urea. 50 mM Tris-HCl(pH 8.0) buffers with 1 M to 4 M urea were used in GFC. Objective was to characterize the structure change with urea concentration. Molecular weight was determined using GF-HPLC and RP-HPLC was used to quantify the unfolded and refolded proteins.

• BMB Reports
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• v.29 no.3
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• pp.183-191
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• 1996

DNA fragments, homologous to the dTDP-D-glucose 4,6-dehydratase gene, obtained from the genomic DNA of Streptomyces antibioticus $T\ddot{u}99$, a producer of the unusual macrolide antibiotic chlorothricin, were cloned and sequenced. This dehydratase gene was designated as oxil. The coding region of the oxil gene is composed of 987 bp, and analysis of the DNA sequence data reveals sequences for the gene products of 329 amino acids (molecular weight of 36,037). The deduced amino acids are 59% identical to the StrE, dTDP-D-glucose 4,6-dehydratase from the streptomycin pathway. The oxil's function was examined by expressing it in E. coli using the T7 RNA polymerase/promoter system (pRSET) to produce an active fusion protein including a his tag. This enzyme shows specificity of substrate, specific only to dTDP-D-glucose.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.15 no.1
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• pp.113-118
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• 2006

In this work, we observed the changes in mass difference according to Al-foam's amount of Ca contents which depends on the viscosity control of fusion, quality of foamed addition, mixing, temperature tests. These are crucial influencing factors in determining foam-metal's size in the manufacturing process. In order to obtain the specimen, we changed the specific gravity from 0.2 to 0.3 for the study of the light weight, and obtained the optimal values of specific gravity, and then showed the mechanical characteristics of ultra-lightweight metal according to the changing mass. The optimal conditions for aluminum foam is when the addition of Ca content in $1.5wt\%~2.0wt\%$

• BMB Reports
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• v.28 no.3
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• pp.275-280
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• 1995

The respiratory burst oxidase of neutrophils is a multicomponent enzyme, domant in resting cells, that catalyzes the reduction of oxygen to $O_{2}^{-}$ at the expense of NADPH. In the resting neutrophil, some of the components of the oxidase, including proteins p47 and p67, are in the cytosol, while the rest are in the plasma membrane. Recent evidence has suggested that at least some of the cytosolic oxidase components exist as a complex. The cytosolic complex with a molecular weight of ~240 kDa was found to bind to blue-agarose and 2',5'-ADP-agarose, which recognize nucleotide requiring enzymes. In order to identify the nucleotide binding component of the cytosolic complex we purified recombinant p47 and p67 fusion proteins using the pGEX system. Pure recombinant p47 was retained completely on 2',5'-ADP-agarose, whereas pure recombinant p67 did not bind to these affinity beads. On the basis of these results, we infer that p47 may contain the nucleotide binding site.

• Microbiology and Biotechnology Letters
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• v.25 no.2
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• pp.137-143
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• 1997

Porcine transforming growth factor-$\beta$1 (TGF-$\beta$1) was expressed in Escherichia coli using cDNA of TGF-$\beta$1 and glutathione S-transferase (GST) fusion vector pGEX-1$\lambda$T. An ApoI-Tth111I fragment of cDNA which correspond to the amino acid residues from 123 to 390 of the precursor TGF-$\beta$1 was inserted into EcoRI-Tth111I digested pGEM#-l$\lambda$T and the recombined plasmid was named pGET-12. Gene products from the cloned regions of the recombinant plasmids pGET-12 was not detected in soluble fraction of cell free extract but detected in insoluble fraction. The solubilization of insoluble gene product was achieved by the treatment of N-laurylsarcosine. Molecular weight of partially purified proteins determined by electrophoresis was same as expected from cloned fragment. The ELISA test results of the purified proteins showed that immunologically detectable epitope was preserved in recombinant protein.

• Journal of Microbiology and Biotechnology
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• v.21 no.10
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• pp.1081-1087
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• 2011

An internally radiating photobioreactor was applied for the production of astaxanthin using the unicellular green alga Haematococcus pluvialis. The cellular morphology of H. pluvialis was significantly affected by the intensity of irradiance of the photobioreactor. Small green cells were widespread under lower light intensity, whereas big reddish cells were predominant under high light intensity. For these reasons, growth reflected by cell number or dry weight varied markedly with light conditions. Even under internal illumination of the photobioreactor, light penetration was significantly decreased as algal cells grew. Therefore, we employed a multistage process by gradually increasing the internal illuminations for astaxanthin production. Our results revealed that a multistage process might be essential to the successful operation of a photobioreactor for astaxnthin production using H. pluvialis.

• Journal of Welding and Joining
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• v.33 no.6
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• pp.36-41
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• 2015

In the automotive industry, TWB hot stamping process is broadly adapted to reduce weight of the car and improve fuel efficiency. However, the process faces a problem of weakened strength of the welded zone after hot stamping process, due to the fact that Al-Si elements of the coating layer penetrating the welded zone. In this study, filler wires with high percentages of carbon and manganese is adapted during laser welding process to secure the strength of the fusion zone. In addition, wire feeding speed and laser welding speed are optimized by sample test.

• The Transactions of the Korean Institute of Electrical Engineers D
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• v.51 no.8
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• pp.335-343
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• 2002

A segment matching algorithm in stereo vision via the fusion of multiple features on long edge segments is proposed. One problem of the previous segment matching algorithm is the similarity among the segments caused from its short length. In the proposed algorithm, edges are composed of longer segments which are obtained by breaking the edges only at the locations with distinguished changes of the shape. Such long segments can contain extra features such as curvature ratio and length of segments which could not be included in shorter ones. Use of such additional features enhances the matching accuracy significantly To fuse multiple features for matching, weighting value determination algorithm which is computed according to the degree of the contribution of each factor is proposed. The stereo matching simulations with the proposed algorithm are done about various images and their results are included.