• 생약학회지
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• 제48권4호
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• pp.268-272
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• 2017

Three tricyclic pyridone alkaloids, (-)-4,6'-anhydrooxysporidinone (1), (-)-6-deoxyoxysporidinoCne (2), and (-)-sambutoxin (3) were isolated from cultures of Fusarium lateritium SSF2 derived from the fruits of Cornus officinalis. Chemical structures of the isolated compounds were determined by spectroscopic method and comparisons with reported data values. The compounds 1-3 were isolated for the first time from this fungal strain.

• 식물병연구
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• 제23권3호
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• pp.283-287
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• 2017

Fusarium lateritium에 의해 발생하는 뽕나무 눈마름병이 전북지역 뽕나무 주산지인 부안지역을 중심으로 발생하였다. 눈마름병이 걸린 가지는 병반 중심부가 약간 오목한 상태의 둥근타원형의 병반을 형성하며 병반 위에 주황색 작은 입자들이 밀생하였다. 뽕나무 가지 위에 병반이 형성된 위쪽으로는 눈이 검게 변하고 말라서 눈이 틔지 않고 말라 죽거나 생육을 약화시키고 심한 경우 결과지가 검게 변하고 말라서 수량 감소를 초래하기도 한다. 뽕나무 눈마름병은 연차 간 발생정도 차이가 심하여 2013년도 전라북도에서 평균적으로 3.2% 발생을 하였으나 2014년도에는 발생한 포장을 찾기가 어려울 정도로 발생이 없었다. 발생차이의 원인을 찾기 위해 재배 중 기상조건을 조사분석한 결과 봄철의 온도가 낮거나 겨울철 영하이하로 경과하는 일수가 증가하는 조건에서 병 발생이 증가하고, 수확하고 난 결과지를 제거한 후에 강우량이 많고 일조시수가 낮은 조건이 경과할 경우 이듬해 발생이 증가하는 것으로 분석되었다. 그러므로 눈마름병이 다발생 할 수 있는 조건이 경과되었을 때는 적용 약제를 예방적으로 살포하여 피해를 최소화하여야 할 것이다.

• The Plant Pathology Journal
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• 제26권1호
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• pp.32-36
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• 2010

Twelve fungal isolates out of 123 isolates obtained from cysts and soils of potato cyst nematode (PCN)-infested fields in Central Java, Indonesia had parasitic abilities of over 50% on PCN eggs or females (cysts) in vitro pathogenicity tests. Cultural and morphological characters and DNA sequences of ribosomal genes in ITS region revealed that they were four isolates of Gliocladium (Trichoderma) virens, three isolates of Fusarium oxysporum, one of F. lateritium, one of Penicillium tritinum and two of Taralomyces spp. A hundred percent infections occurred in eggs or cysts by three fungal isolates G. virens, F. oxysporum and P. oxalicum, suggesting that these fungi may have a good potential for the PCN biocontrol. Especially, G. virens isolates, which occurred most frequently in the PCN-infested potato fields and are known to be highly adaptable to varying habitats, may be developed as reliable agents for controlling PCN with both egg- and cyst-parasitic capabilities and with high ecological adaptabilities.

• Journal of Microbiology and Biotechnology
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• 제19권12호
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• pp.1688-1694
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• 2009

A novel strain of fluorescent pseudomonad (PB-St2) was isolated from surface-sterilized stems of sugarcane grown in Pakistan. The bacterium was identified as Pseudomonas aurantiaca on the basis of 16S rRNA gene sequence analysis and results from physiological and biochemical characteristics carried out with API50 CH and QTS 24 bacterial identification kits. Assays using substrate-specific media for enzymes revealed lipase and protease activities but cellulase, chitinase, or pectinase were not detected. The bacterium was unable to solubilize phosphate or produce indole acetic acid. However, it did produce HCN, siderophores, and homoserine lactones. In dual culture assays on agar, the bacterium showed antifungal activity against an important pathogen of sugarcane in Pakistan, namely Colletotrichum falcatum, as well as for pathogenic isolates of Fusarium oxysporium and F. lateritium but not against F. solani. The antifungal metabolites were identified using thin-layer chromatography, UV spectra, and MALDI-TOFF spectra and shown to be phenazine-1-carboxylic acid (PCA), 2-hydroxyphenazine (2-OH-PHZ), and N-hexanoyl homoserine lactone (HHL) (assessed using only TLC data). The capacity of this bacterium to produce HCN and 2-OH-PHZ, as well as to inhibit the growth of C. falcatum, has not been previously reported.