• KSBB Journal
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• 제28권5호
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• pp.303-309
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• 2013

The extract from Lysimachia foenum-graecum (LFE) has been known to possess various instructive characters including anti-oxidant, anti-obesity, fungicidal activities. However, the accurate mechanism of those effects of LFE is not well known. In that respect, we evaluated the apoptotic effect and anti-cancer efficacy of extracts of LFE in MCF-7 breast cancer cells. In this study, we hypothesized that LFE may exert cancer cell apoptosis through regulating p53 and mitochondria-mediated apoptotic proteins. And this substance can generate ROS to cause free radical-induced apoptosis. Accordingly, the generation of ROS by LFE triggers the activation of p53 which are accompanied by pro-apoptotic protein activation and suppression of pro-survival proteins. We determined with MTT assay, flow cytometry for detection of intracellular ROS and Annexin V-PI staining, Western blotting. Consequently, our researches demonstrated that the treatment of LFE to breast cancer cells resulted in an activation of p53, Puma, Bax, cleaved-PARP and an inhibition of Bcl-2 expressions.

• 한국응용곤충학회지
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• 제10권1호
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• pp.7-12
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• 1971

1. 인삼묘포에 Captan, Difolatan, Zineb, Maneb와 PCNB를 일주일 간격으로 토양관주 하였다. 처리하기전과 2회 및 4회처리후에 각각 Rhizoctonia solani, Pythium debaryanum, Fusarium 그리고 Trichoderma 균총의 숫적 (수적)변화를 Boosalis의 특별잔사법에 의해서 조사하였다. 우리나라에서 인삼(Panax ginseng)의 모잘록병균으로서 Pythium debaryanum을 처음으로 기술하였다. 2. Rhizoctonia solani, Pythium debaryanum의 수는 토양관주에 관계없이 시일이 경과함에 따라서 점차로 감소하였고 Fusarium 및 Trichoderma는 오히려 증가하였다. Rhizoctonia solani의 수는 PCNB에 의해서 현저하게 감소하였고 다른 약제에 의해서도 대조구 보다 감소하였다. 3, Pythium debaryenum은 Zineb, Maneb, Captan, Difolatan의 순으로 고 수가 증가하는 반면 Fusarium은 일정한 경향을 볼 수 없었다. Tichroderma를 제외한 모든 균의 숫적 변화는 시일의 경과에 대하여 $1\%$ 수준에서 유의성이 있었다. 4. 토양관주에 의한 약해는 Maneb, Zineb, Captan 구에서 나타났고 묘삼 뿌리의 생체 무게는 Difolatan, PCNB, Maneb구에서 대조구 보다 증가하였다.

• 한국환경농학회지
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• 제29권3호
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• pp.300-303
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• 2010

본 연구에서는 갈색여치의 친환경적 방제를 위해 곤충병원성 진균인 백강균(B. bassiana)의 병원력에 대한 실험을 수행하였다. 실험에 이용된 모든 균주에서 60% 이상의 병원력을 나타냈으며, 살충시간 또한 10일 이내로 짧았다. 따라서 백강균은 갈색여치의 생물학적 방제원으로 이용 가능성이 높다고 판단할 수 있다.

• 식물병연구
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• 제10권1호
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• pp.69-72
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• 2004

Carbosulfan과 합제로 개발한 isoprothiolane과 tricyclazole, 그리고 probenazole을 벼 육묘상자에 처리하고, 처리 당일에 본답에 이앙한 후, 잎도열병과 목도열병에 대한 방제 효과를 조사하였다. 세 가지의 살균제는 모두 이앙하고 3개월 후까지도 잎도열병을 방제할 수 있었다. 특히 tricyclazole 합제의 경우에는 시간이 경과하면서 그 효과가 더 상승하였으며, 9월 6일 조사 때에 85.5％의 잎도열병 방제 효과를 보였다. 목도열병에 대한 효과는 probenazole이 가장 우수하였는데, 10월 14일에 조사한 probenazole 처리구의 목도열병 이병수율은 6.9％로 78.7％의 무처리구에 비하여 91.2％의 방제 효과를 보였다. Isoprothiolane과 tricyclazole 합제는 잎도열병보다 목도열병에 대한 방제 효과가 저조하였으며, 두 처리구는 각각 47.5％와 61.1％의 효과를 보였다. 이상의 결과처럼 침투이행효과가 우수한 살균제가 벼의 육모상 처리제로 개발이 가능해진다면 포장에서의 도열병 방제가 효과적이고 간편하게 수행될 수 있을 것이다.

• 농약과학회지
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• 제2권1호
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• pp.12-17
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• 1998

버드나무 껍질중의 배당체인 salicin으로부터 유래된 salicylic acid와 그 유사화합물인 3-hydroxy benzoic acid의 질산화된 중간체를 출발물질로 하여 몇몇의 알콜과 에스테르화반응을 거쳐 유기인계 및 카바메이트계 화합물의 전구체를 합성하였다. 합성된 전구체의 수산기에 diethylchlorophosphate와 methyl isocyanate를 반응시켜 유기인계 및 카바메이트계 화합물 11종을 합성하였다. 합성된 화합물은 살충, 살균활성실험을 실시하였다. 유기인계 화합물의 벼멸구에 대한 활성은 500 ppm의 농도에서 O-(2-carbomethoxy-4-nitro phenyl) O,O-diethylphosphate 화합물이 96%의 살충력을 보였다. 반면 carbamate 화합물의 경우는 500ppm농도에서 살충력이 전혀 나타나지 않았다. 살균효과는 유기인계 화합물인 경우 도열병에 대해서 ester에 관계없이 250 ppm농도에서 95% 이상의 방제가를 나타냈으며, 그 이외의 병원균에서는 낮은 방제가를 보였다.

• 한국식물병리학회지
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• 제11권1호
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• pp.9-16
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• 1995

Undiluted neoasozin solution (6.5% a.i.), an arsenic fungicide, was sprayed on 169 cankers of apple trees from early March to September in 1987 twice at intervals of one week without scraping off the affected barks. Among the treated cankers, 79.9% ceased to grow within 1∼7 weeks, 13.0% showed partial development, and 7.1% grew continuously to girdle the branches. The partially developed cankers, however, could also be cured by an additional spray after slightly piercing at the edge of cankers to facilitate the penetration of the chemical. When the canker growth was blocked, cankers were encircled by cracks developed at the marginal area of the cankers. If the cracks developed once, very few cankers grew beyond them. The above results suggest that the crack development may be the consequence of the host defense activity to wall off the pathogen. In addition to the curative efficacy, the neoasozin solution inhibited sporulation of the pathogenic fungus almost completely. However, the pathogen survived for more than three months in some cankers that externally appeared to be cured, suggesting that an indirect mode of action of the chemical against apple Valsa canker seems to be still more persuasive than the direct fungicidal effect. In the final examination conducted in the mid April of the next year, 72.7% of the cankers were completely cured by the two successive neoasozin treatments. Moreover the cure rate became 83.1% if that of partially developed cankers which were also completely cured by an additional treatment was also taken into account. Since 1989 when this method was widely applied in apple orchards in Korea, apple Valsa canker has been effectively controlled to reach a tolerable level.

• Mycobiology
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• 제34권4호
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• pp.219-229
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• 2006

Seventeen microbial species including 10 fungal taxa, two yeasts and five bacteria, were isolated from freshly prepared orange, guava and banana juices kept in open bottles at room temperature for 7 days. Eight different essential oils, from local herbs, were tested for their antimicrobial activity against these test organisms. The essential oils of Cymbopogon citratus, Ocimum basilicum and Origanum majorana were found to be highly effective against these microorganisms. Aspergillus niger, A. flavus and Saccharomyces cerevisiae, the most prevalent microorganisms in juice, showed the highest resistance against these essential oils. GC-MS analysis showed that while e-citral, a'-myrcene, and z-citral represent the major components (75.1 %) of the essential oil of Cymbopogon citratus; bezynen,l-methyl-4-(2-propenyl), 1,8-cineole and trans-a'-bisabolene were the main components (90.6%) of Ocimum basilicum; whereas 3-cyclohexen-l-0l,4-methyl-l(l-methylethyl)-(CAS), c-terpinene and trans-caryophyllene represent the major components (65.1%) of Origanum majorana. These three essential oils were introduced into juices by two techniques namely, fumigation and direct contact. The former technique showed more fungicidal effect than the latter one against A. flavus, A. niger, and S. cerevisiae. The essential oil of Cymbopogon citratus by comparison to other test oils showed the strongest effect against these fungi with a minimum inhibitory concentration of $1.5\;{\mu}l/ml$ medium and a sublethal concentration of $1.0\;{\mu}l/ml$. The antimicrobial activity of this oil is thermostable at $121^{\circ}C$ for 30 min.

• Journal of Microbiology and Biotechnology
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• 제4권4호
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• pp.296-304
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• 1994

Antibiotic substances were produced by Bacillus subtilis YB-70, a potential biocontrol agent found to suppress root-rot of eggplant (Solanum melonggena L) caused by Fusarium solani, in a dextrose glutamate medium and isolated by isoelectric precipitation. Partial purification was performed by column chromatography on silica gel with two solvent systems: chloroform-methanol and methanol-chloroform-water as eluting solvents, This active fraction YBS-1 s contained antifungal activity were soluble in ethanol, methanol, and water, but were not soluble in other solvents including acetone, butanol, ethyl ether, dimethylformamide, propanol, and etc. High performance liquid chromatography and thin layer chromatographic separation of YBS-1s showed that they have been composed of three biological active bands that were named YBS-1A, -1B, and -1C. The substances were stable to heat and resistant to protease. YBS-1s were active against a wide range of plant pathogenic fungi but did not inhibit the growth of bacteria and yeasts. They were not only fungicidal but also fungistatic against chlamydospores of F. solani. The $ED_{50}$ values for the chlamydospore germination and the germ-tube growth of F. solani were $O.725\mu\textrm{m}/ml\;and\;O.562\mu\textrm{m}/ml$, respectively. Microscopic observations proved the substances restricted the growth of phytopathogenic fungus F. solani by spore burst followed by dissolving of its germ-tube, and caused abnormal hyphal swelling after application to chlamydospores or growing hyphae. Cultural filtrate of B; subtilis YB-70 also suppressed the development of root-rot of eggplant in pot tests.

• 대한화학회지
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• 제54권4호
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• pp.429-436
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• 2010

Guanine (1)으로부터 생물활성이 있는 halogenopurines계 화합물을 합성하였다. Guanine을 acetic anhydride와 반응시켜서 2,9-diacetylguanine (2-1)을 합성하여 얻어진 화합물을 $POCl_3$와 반응시켜서 화합물 3a를 합성하고, 다음 단계에서 2-amino-6-halogenopurines (3b-d)를 합성하였다. 2-Halogenopurines (2-2a-d, 4-2a-d, 5a-d)을 2-amino-6-substituted purines (1, 3a, 4-1)로부터 효율적으로 합성한 후에, 새로운 화합물인 2-2a, 2-2c, 2-2d, 4-2c, 4-2d, 5b, 5c 및 5d를 합성하였다. 합성한 화합물의 구조를 원소분석, $^1H$ NMR, mass spectral data로 확인하였으며, 합성한 화합물에 대한 항균 활성을 시험하였다.

• 대한화학회지
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• 제55권1호
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• pp.19-27
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• 2011

2-(Benzothiazol-2-yl)-N'-(2,5-dihydroxybenzylidene)acetohydrazide 에대한새로운일련의 Mn(II), Ni(II), Co(II), Cu(II) 및 Zn(II) 착물을 합성하여 그 특성을 원소분석, IR, UV-vis, $^1H$-NMR, 질량분석, ESR, 자기수자율 및 몰 전기전도도 측정에 의하여 조사하였다. 이들 착물의 기하구조가 사각평면 또는 팔면체임을 분광학적 데이터 및 자기적 측정으로부터 알았다. 이 리간드와 해당착물의 세균에(Aspergillus nigar 및 Fusarium oxysporium) 대한생물학적활성을 조사하였다. 그 결과, 금속착물들은 리간드 및 금속이온 모두에 비해 더 큰 활성을 나타내었다.