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http://dx.doi.org/10.7841/ksbbj.2013.28.5.303

The Extract from Lysimachia foenum-graecum Induces Apoptosis in MCF-7 Breast Cancer Cells  

Lee, Se Hee (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University)
Kim, Guen Tae (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University)
Kim, Jong Il (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University)
Lim, Eun Gyeong (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University)
Kim, In Seop (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University)
Kim, Young Min (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University)
Publication Information
KSBB Journal / v.28, no.5, 2013 , pp. 303-309 More about this Journal
Abstract
The extract from Lysimachia foenum-graecum (LFE) has been known to possess various instructive characters including anti-oxidant, anti-obesity, fungicidal activities. However, the accurate mechanism of those effects of LFE is not well known. In that respect, we evaluated the apoptotic effect and anti-cancer efficacy of extracts of LFE in MCF-7 breast cancer cells. In this study, we hypothesized that LFE may exert cancer cell apoptosis through regulating p53 and mitochondria-mediated apoptotic proteins. And this substance can generate ROS to cause free radical-induced apoptosis. Accordingly, the generation of ROS by LFE triggers the activation of p53 which are accompanied by pro-apoptotic protein activation and suppression of pro-survival proteins. We determined with MTT assay, flow cytometry for detection of intracellular ROS and Annexin V-PI staining, Western blotting. Consequently, our researches demonstrated that the treatment of LFE to breast cancer cells resulted in an activation of p53, Puma, Bax, cleaved-PARP and an inhibition of Bcl-2 expressions.
Keywords
MCF-7 breast cancer cells; Reactive oxygen species (ROS); p53; Apoptosis;
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