• Mycobiology
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• 제34권3호
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• pp.154-157
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• 2006

A fungal isolate collected from infected paprika (Capsicum annuum var. grossum) was characterized as Sclerotinia sclerotiorum based on its ability of sclerotium formation, physiological and molecular properties. When the isolate was grown on potato dextrose agar, oatmeal agar, and malt extract agar, it grew most well on PDA. Optimal temperature and pH for its growth were $25^{\circ}C$ and pH 7, respectively. The fungal isolate produced sclerotia on PDA within 10 days, and the color and shape of the sclerotia were similar to those of S. sclerotiorum. The ITS rDNA regions including ITS1 and ITS2 and 5.8S sequences were amplified using ITS1F and ITS4 primers from the genomic DNAs of the paprika isolate and other known pathogenic S. sclerotiorum isolated from different crops in Korea, and their nucleotide sequences were determined. Sequence comparison analysis showed the ITS rDNA of the paprika isolate shares 100% sequence identity with those of S. sclerotiorum isolated from red pepper, lettuce and a S. sclerotiorum isolate registered in GenBank DNA database. Neighbor joining analysis based on the ITS rDNA sequence revealed the paprika isolate has very close phylogenetic relationships with known Sclerotinia sclerotiorum isolates. This is the first report that S. sclerotiorum has been found associated with paprika rot in paprika growing countries.

• 한국균학회지
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• 제47권3호
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• pp.275-280
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• 2019

청미래덩굴의 근권 토양을 온실에서 배양하여 Glomeromycota에 속하는 균류의 포자를 분리하였다. 분리된 포자는 18S partial rDNA 영역의 DNA 염기서열 분석과, 더 정확한 동정을 위해 추가적으로 ITS 영역과 28S rDNA 영역까지 포함하는 $Kr{\ddot{u}}ger$ fragment 지역의 염기서열을 분석하여 동정하였다. 동정 과정에서 2종의 국내 미기록종 Glomeromycota 균류의 포자를 확인하였으며, 확인된 종은 Diversispora eburnea, Paraglomus laccatum이다. 확인된 미기록종 포자의 형태적 특성 및 계통적 분석의 결과에 대해 기술하였다.

• Journal of Microbiology
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• 제40권4호
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• pp.289-294
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• 2002

The fungal strain SW-3 having antimicrobial activity was isolated from soil of crucified plants in Pocheon, Kyungki-Do, Korea. Strain SW-3 was identified as Alternaria brassicicola by its morphological characteristics, and confirmed by the analysis of the 18S gene and ITS regions of rDNA. The fungus showed a similarity of 99% with Alternaria brassicicola in the 18S rDNA sequence analysis. A. brassicicola has been reported to produce an antitumor compound, called depudecin. We found that strain SW-3 produced antimicrobial metabolites, in addition to depudecin, during sporulation under different growth conditions. The metabolite of the isolated fungus was found to have strong antifungal activity against Microsporium canis and Trichophyton rubrum, and antibacterial activity against Staphylococcus aureus and Pseudomonas aerogenes. The amount and kind of metabolites produced by the isolate were affected by growth conditions such as nutrients and growth periods.

• 한국균학회지
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• 제48권3호
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• pp.251-271
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• 2020

Diversity and community composition of bulb-associated fungi of Fritillaria Cirrhosae Bulbus source plants, which are used in traditional chinese medicine, in the eastern Himalaya-Hengduan Mountains, southwestern China, were estimated based on the internal transcribed spacer rDNA sequence analysis, using host plant species, geographic area, and plant phenology as variables. A total of 1,486 fungal sequences assigned to 251 operational taxonomical units (OTUs) were obtained from the bulbs. Fungal OTUs comprised 96.41% Ascomycotina, 3.52% Basidiomycotina, and 0.07% Zygomycotina. Sordariomycetes, Hypocreales, and Nectriaceae were the most frequent fungal lineages at each taxonomic rank. Fusarium, Ilyonectria, Tetracladium, Leptodontidium, and Tomentella were the top OTU-rich genera. Fusarium sp. 03, Ilyonectria rufa, Fusarium sp. 08, Ilyonectria sp. 03, and Leptodontidium orchidicola 03 represented the most frequent OTUs. Fusarium spp. were the most frequent general taxa. The distribution of fungal community exhibited preferences for host plant species, geographic area, and plant phenology. These findings are the foundation of our research on culturing and active metabolites of bulb-associated fungi of Fritillaria Cirrhosae Bulbus source plants.

• 한국균학회지
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• 제47권2호
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• pp.113-120
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• 2019

경북영주의 산림약용자원연구소에서 재배하고 있는 약용작물 3종(토천궁, 참당귀, 일천궁)의 뿌리에서 내생균을 분리하였다. 분리된 균주는 형태적 특성과 internal transcribed spacer, large subunit rDNA, beta-tubulin 영역의 분자적 분석을 토대로 동정하였다. 연구과정에서 3종의 국내 미기록종 내생균 균주를 확인하였고, 확인된 종은Dactylonectria pauciseptata, Rhizopycnis vagum, Sistotrema sernanderi이다. 미기록종 내생균 균주의 형태적 특성 확인 및 계통 분석 결과에 대해 기술하였다.

• 한국균학회지
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• 제48권2호
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• pp.117-124
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• 2020

In this study, we isolated endophytic fungi from the conifer leaves of Pinus densiflora inhabiting Mt. Gyehwasan in Korea. We identified the isolated fungal strains based on their morphological characteristics and phylogenetic analysis using DNA sequences of the internal transcribed spacer, small subunit rDNA, large subunit rDNA, and β-tubulin region. In the process of this study, we confirmed two endophytic fungi that have not been recorded previously in Korea, Lophiostoma macrostomum and Pestalotiopsis lespedezae. In this report, we described the morphological characteristics of these fungal strains and the results of their molecular analysis.

• The Plant Pathology Journal
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• 제20권4호
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• pp.264-270
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• 2004

Fusarium oxysporum f. sp. fragariae is a fungal pathogen causing strawberry wilt disease. The random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphisms (RFLPs) of intergenic spacer (IGS) region of rDNA were used to identify genetic variation among 22 F. oxysporum f. sp. fragariae isolates. All isolates could be distinguished from each other by RAPD analysis and RFLP of 2.6 kb amplified with primer CNS1 and CNL12 for IGS region of rDNA. Cluster analysis using UPGMA showed eight distinct clusters based on the banding patterns obtained from RAPD and rDNA RFLP. These results indicate that F. oxysporum f. sp. fragariae isolates are genetically distinct from each other, There was a high level genetic variation among F. oxysporum f. sp. fragariae.

• 한국균학회지
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• 제44권4호
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• pp.247-251
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• 2016

본 연구에서는 강원 삼척 및 경북 영주 지역의 하천에서 채집해 온 담수 침전식물체 및 담수 포말에서 균을 분리하여 수생균류 3종을 발굴하였다. 분리된 균을 형태학적 특징 관찰과 internal transcribed spacers rDNA 유전자 분석을 통해 동정한 결과, NNIBRFG329, NNIBRFG339, NNIBRFG19 균주는 각각 Articulospora tetracladia, Margaritispora aquatica, Aquanectria penicillioides이었다. 이들 3종의 균류는 불완전균강에 속하는 담수 서식 균류로 국내 미기록종으로 보고하는 바이다.

• Mycobiology
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• 제42권4호
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• pp.311-316
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• 2014

Lichen studies, including biodiversity, phylogenetic relationships, and conservation concerns require definitive species identification, however many lichens can be challenging to identify at the species level. Molecular techniques have shown efficacy in discriminating among lichen taxa, however, obtaining genomic DNA from herbarium and fresh lichen thalli by conventional methods has been difficult, because lichens contain high proteins, polysaccharides, and other complex compounds in their cell walls. Here we report a rapid, easy, and inexpensive protocol for extracting PCR-quality DNA from various lichen species. This method involves the following two steps: first, cell breakage using a beadbeater; and second, extraction, isolation, and precipitation of genomic DNA. The procedure requires approximately 10 mg of lichen thalli and can be completed within 20 min. The obtained DNAs were of sufficient quality and quantity to amplify the internal transcribed spacer region from the fungal and algal lichen components, as well as to sequence the amplified products. In addition, 26 different lichen taxa were tested, resulting in successful PCR products. The results of this study validated the experimental protocols, and clearly demonstrated the efficacy and value of our KCl extraction method applied in the fungal and algal samples.

• 한국균학회지
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• 제22권4호
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• pp.366-377
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• 1994

Cycloheximide와 nalidixic acid를 각각 처리한 배지에 Aspergillus phoenicis, Rhizopus acidus, Candida albicans를 배양하는 동안에 이들 세포에서 항생물질이 DNA 합성에 어떠한 영향을 미치는 가를 대조구와 비교 분석하였다. Cycloheximide 처리구에서의 DNA 염기 함량은 A. phoenicis에서 대조구에 비해 adenine 20.4%, thymine 43.1%, cytosine 40.9%, guanine 35.3%가 감소되어 purine기, pyrimidine기가 각각 32.2%, 42.7% 억제 현상을 나타내었다. R. acidus에서는 adenine이 34.2%, thymine이 42.1%, cytosine은 38.0%, guanine은 18.1%가 감소됨으로 purine기와 pyrimidine기가 24.1%와 40.0%로 저해되었다. 그리고 C. albicans의 염기 조성은 adenine이 58.3%, thymine이 58.5%로 대조구에 비해 억제되었고 cytosine은 58.1%, guanine은 42.4%가 감소되어 purine기 46.8%, pyrimidine기 58.8%의 억제를 보여주었다. Nalidixic acid 처리구에서는 A. phoenicis에서 adenine 41.6%, thymine 47.1%, cytosine 59.3%, guanine 46.3%가 저해되어 purine기 45.6%, pyrimidine기 57.2%가 감소되었다. R. acidus에서의 염기함량은 adenine 59.1%, thymine 54.7%, cytosine 35.3%, guanine 37.4% 감소로 purine기 45.9%, pyrimidine기 44.9%가 대조구에 비해 억제 효과를 보여주었다. C. albicans에서는 adenine이 60.1%, thymine이 68.6%, cytosine이 60.7%, guanine이 40.0% 저해되어 purine기는 45.8%, pyrimidine기는 63.5% 감소현상을 보였다. 이들 3 균주의 DNA 생합성에서 purine기 보다는 pyrimidine기가 cycloheximide와 nalidixic acid에 의해 뚜렷한 저해 효과를 나타내는 것으로 조사되었다. 본 실험에서 cycloheximide보다는 nalidixic acid가 DNA 생합성에 현저한 억제작용을 하는 것으로 분석되었다.