• Korean Journal of Medicinal Crop Science
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• v.11 no.5
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• pp.358-363
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• 2003

ScopoIamine and hyoscyamine which belong to tropane alkaloids are the pharmaceutically valuable anticholinergic drugs. In order to increase the productivities, the effects of elicitation were investigated during hairy root cultures of Scopolia. parviflora. Biotic elicitors originated from 3 fungi and 1 yeast were prepared as homogenate and supernatant and added to 3-week-old cultures. Both of homogenate and supernatant of Candida albicans elicitors increased the scopolamine production. The production of hyoscyamine was enhanced by homogenate of Fusarium solani and supernatant of C. albicans. Most of the other fungal elicitors were also improved on the tropane alkaloid production compared to non-treatment. Among the elicitors tested, C. albicans was proved the optimal biotic elicitor on tropane alkaloids production. These results will be served mass production of tropane alkaloids by large-scale production.

• Korean Journal Plant Pathology
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• v.12 no.3
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• pp.285-290
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• 1996

3-hydroxy-3-methyglutaryl coenzyme A reductase (HMGR)는 phytoalexin을 포함하는 수 많은 isoprenoid화합물의 생합성을 조절하는 효소이다. 토마토의 경우 sesquiterpenoid phytoalexin류가 식물방어를 위한 반응산물로서 축적되는 것이 알려져 있다. Verticil-lium albo-atrum이나 Fusarium oxysporum으로부터 추출한 elicitor를 토마토의 배양세포에 처리하는 경우 처리량의 증가에 따른 2.7kb 크기의 HMGR mRNA의 상당한 유도증가가 토마토의 HMG2 DNA를 이용한 northern hybridization에 의해 관찰되었다. 토마토의 잎, 뿌리, 줄기 등에 기계적 상해를 가하는 경우에서도 HMGR mRNA는 2단계를 걸쳐 증가함이 관찰되었다. HMGR mRNA는 양 실험의 경우 모두 9시간에서 12시간 사이에서 최대발현됨이 관찰되었다.

• The Plant Pathology Journal
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• v.21 no.3
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• pp.283-287
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• 2005

Since the discovery of generation of $O_2^-$ in plant, many evidence for the oxidative burst (OXB) has been accumulated in various combinations of plant and pathogen or elicitor systems. $O_2^-$ generating system responsible for the OXB was coupled with oxidation of reduced nicotinamide adenine dinucleotide phosphate (NADPH) in microsomal fraction isolated from sliced aged potato tuber slices which were treated by hyphal wall components elicitor from Phytophthora infestans (HWC). We developed new assay method for quantitative measurement of oxygen radical $O_2^-$ by using electron spin resonance (ESR) analysis during elicitor­induced OXB on the surface of plant tissues. The ESR analysis using an $O_2^-$ trapper, Tiron (1,2-dihydroxy-3,5­benzenedisulfonic acid), provided a convenient assay for detecting only $O_2^-$ during elicitor-induced OXB producing various active oxygen species (AOS) on plant tissue surface. Tiron was oxidized to Tiron semiquinon radical by $O_2^-$. Quantity of the radical signal was measured by specific spectra on ESR spectroscopy. The level of $O_2^-$ was high in from surface of potato tuber tissue treated with hyphal cell wall elicitor (HWC) from Phytophthora infestans. There was no secondary OXB induction by $H_2O_2$ treatment in plant.

• Proceedings of the Zoological Society Korea Conference
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• 1999.10b
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• pp.213.1-213
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• 1999

No Abstract, See Full Text

• KSBB Journal
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• v.21 no.5
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• pp.331-335
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• 2006

Suspension cells of Angelica gigas Nakai were cultivated to produce extracellular polysaccharide(ECP) as immunostimulating agents. Effects of environmental conditions such as sucrose and 2,4-dichlorophenoxyacetic acid(2,4-D) concentrations on the growth and production of ECP were studied using suspension cultures of A. gigas Nakai. Final dry cell weight was increased with an increase of initial sucrose concentration from 30 to 60 g/L. The maximum production of ECP(1.2 g/L) was achieved at an initial sucrose concentration of 50 g/L on day 8. High 2,4-D concentration was effective for ECP production but not for cell growth. In addition, various fungal elicitors were investigated for the enhanced production of ECP in A. gigas suspension cultures. Among the tested fungal elicitors, Verticillium dahliae was the most effective for the production of ECP in A. gigas suspension culture.

• Journal of Life Science
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• v.8 no.5
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• pp.604-613
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• 1998

The last enzyme of the sesquiterpen phytoalexin capsidiol synthesis in tobacco cell, 5-epi-aristolochene hydro-xylase which convert 5-epi-aristolochene (EAS) to capsidiol, was cloned by a reverse transcription polymerase chain reaction strategy and cDNA library screening. Cloned CYP-B3 contained high probability amino acid matches to known plant cytochrome P450 sequences and open reading frame with the conserved FxxGxRxCxG heme-binding region. Transcripts of CYP-B3 were not detected in control cells, but induced in elicitor-treated cells. Furthermore, CYP-B3 transcripts were induced by fungal extracts and cellulase but not by other stimuli(chilling, heat shock and 2,4-D). Induction of CYP-B3 transcripts by elicitor treatment was not affected by ancymidol and ketoconazole treat-ments suggesting that an inhibition of hydroxylase activity by Cyt P450 inhibitors resulting from post translational processing event.

• Journal of Microbiology and Biotechnology
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• v.20 no.8
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• pp.1226-1229
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• 2010

Glyceollins, one of the inducible phytoalexins produced by plants, were induced in a number of varieties of Korean soybean through fungal infection. Of the tested soybean varieties, Tae-Kwang, though not the most productive, was found to be currently the most suitable for the induction of glyceollins. Amongst the fungal species, Rhizopus microsporus var. oligosporus was seen to be the most effective elicitor. Halved soybean seeds produced glyceollins upon fungal infection; however, chopped soybeans and homogenized soybeans did not produce significant quantities of glyceollins.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2002.04b
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• pp.186-186
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• 2002

• Proceedings of the Korean Society of Life Science Conference
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• 2003.05a
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• pp.67-67
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• 2003

• Journal of Life Science
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• v.14 no.1
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• pp.38-44
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• 2004

Two genes, SinIFS1 and SinIFS2 from Korean soybean cultivar, Sinpaldalkong known as one of isoflavonerich cultivars, were cloned with PCR and degenerate primers. The sequences of two genes were analyzed with previously reported IFS genes of leguminous plants and their expression pattern in various environmental conditions was surveyed. The genomic clone of SinIFS1 contained 1,828bp nucleotides and encoded a polypeptide of 521 amino acids, and 1912bp nucleotides and a polypeptide of 521 amino acids for SinIFS2. Both genes included several conserved motifs, oxygen binding and activation (A/G-G-X-E/D-T-T/S), ERR triad (E...R....R), and heme binding (F-X-X-G-X-R-X-C-X-G) domain, which are typical in any member of cytochrome P45O superfamily. Very high sequence homology (>98%) was observed in the comparison with other IFSs of legumes. In the northern blot analysis to check the expression and increase of SinIFS1 to various environmental renditions (low temperature, light, dark, UV, and fungal elicitor), the most significant induction, more than 6 times of transcript level compared to the dark treatment as a control, was observed from the fungal elicitor treatment. The next up-regulated expression was from UV treatment (4${\times}$), low temperature and light conditions.