• Journal of Ecology and Environment
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• 제41권9호
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• pp.271-280
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• 2017

Background: Soil microorganisms play key roles in nutrient cycling and are distributed throughout the soil profile. Currently, there is little information about the characteristics of the microbial communities along the soil depth because most studies focus on microorganisms inhabiting the soil surface. To better understand the functions and composition of microbial communities and the biogeochemical factors that shape them at different soil depths, we analyzed microbial activities and bacterial and fungal community composition in soils up to a 120 cm depth at a fallow field located in central Korea. To examine the vertical difference of microbial activities and community composition, ${\beta}$-1,4-glucosidase, cellobiohydrolase, ${\beta}$-1,4-xylosidase, ${\beta}$-1,4-N-acetylglucosaminidase, and acid phosphatase activities were analyzed and barcoded pyrosequencing of 16S rRNA genes (bacteria) and internal transcribed spacer region (fungi) was conducted. Results: The activity of all the soil enzymes analyzed, along with soil C concentration, declined with soil depth. For example, acid phosphatase activity was $125.9({\pm}5.7({\pm}1SE))$, $30.9({\pm}0.9)$, $15.7({\pm}0.6)$, $6.7({\pm}0.9)$, and $3.3({\pm}0.3)nmol\;g^{-1}\;h^{-1}$ at 0-15, 15-30, 30-60, 60-90, and 90-120 cm soil depths, respectively. Among the bacterial groups, the abundance of Proteobacteria (38.5, 23.2, 23.3, 26.1, and 17.5% at 0-15, 15-30, 30-60, 60-90, and 90-120 cm soil depths, respectively) and Firmicutes (12.8, 11.3, 8.6, 4.3, and 0.4% at 0-15, 15-30, 30-60, 60-90, and 90-120 cm soil depths, respectively) decreased with soil depth. On the other hand, the abundance of Ascomycota (51.2, 48.6, 65.7, 46.1, and 45.7% at 15, 30, 60, 90, and 120 cm depths, respectively), a dominant fungal group at this site, showed no clear trend along the soil profile. Conclusions: Our results show that soil C availability can determine soil enzyme activity at different soil depths and that bacterial communities have a clear trend along the soil depth at this study site. These metagenomics studies, along with other studies on microbial functions, are expected to enhance our understanding on the complexity of soil microbial communities and their relationship with biogeochemical factors.

• 한국환경복원기술학회지
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• 제3권1호
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• pp.70-79
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• 2000

Arbuscular mycorrhizal(AM) fungi have significant role for ecosystem structure and function. They are the major component of forest soil ecosystems and critically important for water and nutrient cycling in the system. To understand the ecology of AM fungi the fungal spores were collected, identified and counted in forest soils under various climatic and edaphic conditions. In relation to soil depth 90% of AM fungi spores and mycorrhizas distributed within 15cm soil depth. Number of spores per $100m{\ell}$ forest soil volume was 5 to 36 spores from 1 to 3 fungal species. AM fungal species diversity was higher in warmer climates, and more moist and fertile soils. The most frequently found species were Gigaspora decipiens irrespective of soil moisture and Gi. gigantea irrespective of soil fertility. In the Jeju island the soils of Cryptomeria japonica plantations and Miscanthus sinensis var. purpurascens meadow had more AM spores than the other soils. We suggest AM fungi be considered as keystones species when restoring a disturbed forest ecosystem.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 추계학술대회 및 정기총회
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• pp.48-48
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• 2014

Arbuscular Mycorrhizal Fungi(AMF) is widespread symbiont forming mutualistic relationship with plant root in terrestrial forest in ecosystem. They provide improved absorption of nutrient and water, and enhance the resistance against plant pathogen or polluted soil, therefore AM fungi are important for survival and maintaining of individual or community of plant. For last decade, many studies about the functional variation of AM fungi on host plant growth response were showed that different geographic isolates, even same species, have different effect on host plant. However, little was known about functional variation of AM fungal isolates originated single population, which provide important insight about intraspecific diversity of AMF and their role in forest ecosystem. In this study, four AM fungal isolates of Rhizophagus clarus were cultured in vitro using transformed carrot (Daucus carota) root and they showed the difference between isolates in ontogenic characteristics such as spore density and hyphal length. The plant growth response by mycorrhizas were measured also. After 20 weeks from inoculation of these isolates to host plants, dry weight, Root:Shoot ratio, colonization rates and N, P concentration of host plant showed host plant was affected differently by AM fungal isolates. This results suggest that AM fungi have high diversity in their functionality in intraspecific level, even in same population.

• 한국응용과학기술학회지
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• 제37권6호
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• pp.1489-1495
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• 2020

Gotjawal, Jeju Island, Korea is a lava-formed forest with low soil content that is unique in the world. Around 6 percent of the Jeju Iand is classified as a lava-based specific forest. The forest has been gradually disappearing during the last several decades, with approximately one half having been destroyed. Our study undertakes a detailed analysis of the landscape of the lava subsidence, and describes the fungi, vegetation, and soils of Cheongsu Gotjawal. Soil samples from the Gotjawal were collected, and soil analyses as well as pyrosequencing of the internal transcribed spacer gene for fungal communities were performed. Soil fungal communities are represented by Discisedars, Fusarium, Pleochaeta, and Fuscoporia genera. Endemic vegetation of the Gotjawal includes the plants Pleris critical, Machilus japonica, Quercus glauca, Arachniodes aristata, and Neocheiropteris ensata. Results of soil analysis indicate sandy loam with 31.70% organic matter, and 1.36 mg/kg of total nitrogen. This fundamental information can help understand the invaluable and unique nature of Cheongsu Gotjawal, and the necessity for more studies on Gotjawal.

• 한국균학회지
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• 제43권4호
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• pp.281-285
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• 2015

석회유황합제의 배나무 동계방제 수단으로써 효과를 과학적으로 증명하기 위해 배 과수원에서 1차 전염원의 역할을 하는 낙엽에 석회유황합제를 처리한 후 낙엽에 존재하는 진균의 다양성 변화를 확인하였다. 물이 처리된 대조구와 석회유황합제 처리구의 낙엽에서 진균 genomic DNA를 추출한 다음 rDNA internal transcribed spacer (ITS) 영역의 염기서열 분석을 통해 진균들을 동정한 결과, 대조구와 석회유황합제 처리구에서 자낭균문(Ascomycota)과 담자균문(Basidiomycota)에 속하는 다양한 종류의 진균들이 동정되었으나 동정되는 진균의 수와 비율은 서로 다르게 나타났다. 특히 배 과피얼룩병을 일으키는 Alternaria속과 Cladosporium속의 진균은 대조구에 비해 석회유황합제 처리구에서 동정되는 비율이 대폭 감소되었으며, Phomopsis속의 진균은 대조구에서는 동정되었으나 석회유황합제 처리구에서는 동정되지 않았다. 이러한 결과는 석회유황합제 처리가 배 낙엽에 존재하는 진균 다양성의 변화에 영향을 미쳤으며 동계방제로서 역할을 수행할 수 있음을 시사한다.

• Mycobiology
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• 제30권1호
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• pp.18-21
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• 2002

Four arbuscular mycorrhizal fungal(AMF) inocula collected from three arable sites in Korea were used to determine plant growth, mycorrhizal root colonization rate and spore production in three different host plant species; Sorghum bicolor, Allium fistulosum, Tagetes patula. Growth of plant treated with AMF differed from those without AMF. Different AMF inocula showed significantly different root colonization rates and spore production of AMF on the wild plants, A. fistulosum and T. patula, but did not on the cultivated plant, S. bicolor. Results suggested that indigenous mycorrhizal fungal community would be important factors in mycorrhizal symbiosis, and play important roles in the plant succession.

• Mycobiology
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• 제42권4호
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• pp.401-404
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• 2014

A new recorded species of Mortierella was recovered during the investigation of fungal communities in soil samples collected from different locations of Gangwon-do, Korea. The species was identified and described as Mortierella alpina on the basis of phylogenetic analysis of internal transcribed spacer sequences and morphological characteristics. This species has not been officially reported from Korea thus far.

• 한국균학회지
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• 제37권2호
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• pp.134-138
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• 2009

This study was conducted to investigate colonization of ectomycorrhizal fungi(ECM) in roots of Abies koreana which is an endemic and endangered species in Korea. Roots of A. koreana were collected at Mt. Halla. ECM root tips were classified using morphotyping and identified using sequences of internal transcribed spacer (ITS) region of the fungal rDNA. Total 8 species of ECM fungi were identified from roots of 11 seedlings of A. koreana : Cenococum geophilum, Russula brevipes, 2 species of Russula, 2 species of Thelephora, Cortinarius camphorates and 2 species of Helotiales. These species were known to be typical ectomycorrhizal fungi found in coniferous mature forests.

• 한국균학회지
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• 제44권3호
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• pp.132-137
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• 2016

A unrecorded hyphomycete species of Phialocephala was isolated for the first time during the investigation of fungal community in the soil samples collected from different regions of Korea. The fungal isolate was identified as Phialocephala lagerbergii, based on the morphological characteristics and phylogenetic analysis of the ribosomal DNA sequence. In addition, cultural and micro-morphological features were described in detail.

• The Plant Pathology Journal
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• 제36권3호
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• pp.193-203
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• 2020

Volatile compounds (VOCs) are not only media for communication within a species but also effective tools for sender to manipulate behavior and physiology of receiver species. Although the influence of VOCs on the interactions among organisms is evident, types of VOCs and specific mechanisms through which VOCs work during such interactions are only beginning to become clear. Here, we review the fungal volatile compounds (FVOCs) and their impacts on different recipient organisms from perspective of distinct lifestyles of the filamentous fungi. Particularly, we discuss the possibility that different lifestyles are intimately associated with an ability to produce a repertoire of FVOCs in fungi. The FVOCs discussed here have been identified and analyzed as relevant signals under a range of experimental settings. However, mechanistic insight into how specific interactions are mediated by such FVOCs at the molecular levels, amidst complex community of microbes and plants, requires further testing. Experimental designs and advanced technologies that attempt to address this question will facilitate our understanding and applications of FVOCs to agriculture and ecosystem management.